{"id":16958,"date":"2017-12-14T06:24:57","date_gmt":"2017-12-13T22:24:57","guid":{"rendered":"\/\/www.gsbet888.com\/?p=16958"},"modified":"2019-08-02T18:52:18","modified_gmt":"2019-08-02T10:52:18","slug":"%e8%84%b1%e6%b0%a2%e4%b8%99%e6%b0%a8%e9%85%b8-%e9%80%89%e6%8b%a9%e6%80%a7%e8%9b%8b%e7%99%bd%e8%b4%a8%e4%bf%ae%e9%a5%b0-dha-selective-protein-modification","status":"publish","type":"post","link":"\/\/www.gsbet888.com\/reactions\/2017\/12\/%e8%84%b1%e6%b0%a2%e4%b8%99%e6%b0%a8%e9%85%b8-%e9%80%89%e6%8b%a9%e6%80%a7%e8%9b%8b%e7%99%bd%e8%b4%a8%e4%bf%ae%e9%a5%b0-dha-selective-protein-modification.html","title":{"rendered":"\u8131\u6c22\u4e19\u6c28\u9178-\u9009\u62e9\u6027\u86cb\u767d\u8d28\u4fee\u9970 Dha-Selective Protein Modification"},"content":{"rendered":"<p><strong>\u8131\u6c22\u4e19\u6c28\u9178(dehydroalanine, Dha)<\/strong>\u662f\u7531\u4e1d\u6c28\u9178\u6216\u8005\u534a\u80f1\u9170\u80fa\u8f6c\u5316\u6765\u7684\u7279\u6b8a\u6c28\u57fa\u9178\u3001\u7531\u4e8e\u5176\u5177\u6709Michael\u52a0\u6210acceptor\u7ed3\u6784\uff0c\u6240\u4ee5\u4f5c\u4e3a\u86cb\u767d\u8d28\u7684\u4fee\u9970\u8d77\u70b9\u4e5f\u53d7\u5230\u4e00\u5b9a\u7684\u5173\u6ce8\u3002\u4e5f\u5c31\u662f\uff0c\u5927\u90e8\u5206\u7684\u6c28\u57fa\u9178\u6b8b\u57fa\u5bf9\u4eb2\u6838\u8bd5\u5242\u6216\u8005\u81ea\u7531\u57fa\u663e\u60f0\u6027\u3001\u5229\u7528\u8fd9\u4e00\u7279\u6027\uff0c\u53ef\u4ee5\u9009\u62e9\u6027\u7684\u5bf9\u7ffb\u8bd1\u540e\u7684\u542b\u6709\u8131\u6c22\u4e19\u6c28\u9178\u7684\u86cb\u767d\u8d28\u5176\u8fdb\u884c\u4fee\u9970\u3002<\/p>\n<h4>\u57fa\u672c\u6587\u732e<\/h4>\n<ul>\n<li>Zhu,Y.; van der Donk, W. A. <em>Org. Lett.<\/em> <strong>2001<\/strong>, <em>3<\/em>, 1189. DOI: <a href=\"http:\/\/dx.doi.org\/10.1021\/ol015648a\">10.1021\/ol015648a<\/a><\/li>\n<li>Galonic, D. P.; van der Donk, W. A.; Gin, D. Y. <em>Chem. Eur. J.<\/em> <strong>2003<\/strong>, <em>9<\/em>, 5997. DOI: <a href=\"http:\/\/dx.doi.org\/10.1002\/chem.200305290\">10.1002\/chem.200305290<\/a><\/li>\n<li>Levengood, M. R.; van der Donk, W. A. <em>Nat. Protoc.<\/em> <strong>2007<\/strong>, <em>1<\/em>, 3001. doi:<a href=\"http:\/\/dx.doi.org\/10.1038\/nprot.2006.470\">10.1038\/nprot.2006.470<\/a><\/li>\n<li>Wright, T. H.; Davis, B. G. <em>Nat. Protoc.<\/em> <strong>2017<\/strong>, <em>12<\/em>, 2243. doi:<a href=\"http:\/\/dx.doi.org\/10.1038\/nprot.2017.087\">10.1038\/nprot.2017.087<\/a><\/li>\n<\/ul>\n<h5>&lt;Installation of Dha&gt;<\/h5>\n<ul>\n<li>Chalker, J. M.; Gunnoo, S. B.; Boutureira, O.; Gerstberger, S. C.; Fern\u00e1ndez-Gonz\u00e1lez, M.; Bernardes, G. J. L.; Griffin, L.; Hailu, H.; Schofield. C. J.; Davis, B. G. <em>Chem. Sci.<\/em> <strong>2011<\/strong>, <em>2<\/em>, 1666. DOI: <a href=\"http:\/\/dx.doi.org\/10.1039\/c1sc00185j\">10.1039\/c1sc00185j<\/a><\/li>\n<\/ul>\n<h5>&lt;Review&gt;<\/h5>\n<ul>\n<li>Chalker, J. M.; Bernardes, G. J. L.; Lin, Y. A.; Davis, B. G. <em>Chem. Asian J.<\/em> <strong>2009<\/strong>, <em>4<\/em>, 630. DOI: <a href=\"http:\/\/dx.doi.org\/10.1002\/asia.200800427\">10.1002\/asia.200800427<\/a><\/li>\n<li>Gunno, S. B.; Madder, A. <em>ChemBioChem<\/em> <strong>2016<\/strong>, <em>17<\/em>, 529. DOI: <a href=\"http:\/\/dx.doi.org\/10.1002\/cbic.201500667\">10.1002\/cbic.201500667<\/a><\/li>\n<\/ul>\n<h4>\u8131\u6c22\u4e19\u6c28\u9178\u7684\u751f\u6210\u6cd5<\/h4>\n<p>\u81ea\u7136\u754c\u4e2d\u662f\u901a\u8fc7\u9176\u50ac\u5316\u4e1d\u6c28\u9178\u8fdb\u884c\u8131\u6c34\u751f\u6210\u7684[1]\u3001\u800c\u5728\u5316\u5b66\u5408\u6210\u4e0a\u662f\u7528\u53cd\u5e94\u6027\u66f4\u9ad8\u7684\u534a\u80f1\u9170\u80fa\u4f5c\u4e3a\u5e95\u7269\u8fdb\u884c\u8131\u786b\u5236\u5907\u7684\u3002\u5230\u76ee\u524d\u4e3a\u6b62\uff0c\u5f00\u53d1\u51fa\u4e86\u591a\u79cd\u8bd5\u5242\u4ee5\u8fbe\u5230\u6b64\u76ee\u7684\uff0c\u6700\u7b80\u4fbf\u4e4b\u4e00\u662fDavis\u7b49\u4eba\u5f00\u53d1\u7684\u4e0b\u56fe\u6240\u793a\u7684\u624b\u6cd5\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-16960 size-full\" src=\"https:\/\/assets.www.gsbet888.com\/uploads\/2017\/12\/Dha_conjugation_2-e1509693840608.png\" alt=\"\" width=\"503\" height=\"125\" srcset=\"https:\/\/assets.www.gsbet888.com\/uploads\/2017\/12\/Dha_conjugation_2-e1509693840608.png 503w, https:\/\/assets.www.gsbet888.com\/uploads\/2017\/12\/Dha_conjugation_2-e1509693840608-300x75.png 300w\" sizes=\"(max-width: 503px) 100vw, 503px\" \/><\/p>\n<p>\u4ee5\u524dchem-station\u62a5\u9053\u8fc7\u7684\u5229\u7528<a href=\"\/\/www.gsbet888.com\/reactions\/2016\/10\/msh%e8%af%95%e5%89%82-msh-reagent.html\">MSH\u8bd5\u5242<\/a>\u7684\u624b\u6cd5[3]\u3001\u7531\u4e8e\u4f1a\u5f15\u8d77\u80fa\u57fa\u7684\u8131\u80fa\u57fa\u5316\u53cd\u5e94\uff0c\u6240\u4ee5\u5b58\u5728\u4ea4\u53c9\u53cd\u5e94\u6027\u7684\u95ee\u9898[2]\u3002<\/p>\n<h4>\u5bf9\u8131\u6c22\u4e19\u6c28\u9178\u7684\u5e94\u7528\u5b9e\u4f8b<\/h4>\n<p>\u5927\u90e8\u5206\u7684case\u90fd\u662f\u4e0e\u53cd\u5e94\u6027\u9ad8\u7684\u786b\u70f7\u8fdb\u884c\u8fc8\u514b\u5c14\u52a0\u6210\u53cd\u5e94\uff0c\u4e0b\u9762\u7ed9\u51fa\u4e86\u56db\u4e2a\u5178\u578b\u4f8b\u5b50[4]\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-16961 size-full\" src=\"https:\/\/assets.www.gsbet888.com\/uploads\/2017\/12\/Dha_conjugation_3-e1509693892987.png\" alt=\"\" width=\"542\" height=\"260\" srcset=\"https:\/\/assets.www.gsbet888.com\/uploads\/2017\/12\/Dha_conjugation_3-e1509693892987.png 542w, https:\/\/assets.www.gsbet888.com\/uploads\/2017\/12\/Dha_conjugation_3-e1509693892987-300x144.png 300w\" sizes=\"(max-width: 542px) 100vw, 542px\" \/><\/p>\n<p>\u4f46\u662f\u8fd0\u7528\u4e0a\u8ff0\u6761\u4ef6\u5728\u53cd\u5e94\u540e\uff0c\u80af\u5b9a\u4f1a\u7559\u6709\u786b\u919a\u90e8\u4f4d\uff0c\u800c\u8fd9\u4f1a\u5bfc\u81f4\u7ffb\u8bd1\u540e\u4fee\u9970\u7684\u518d\u73b0\u6027\u95ee\u9898\u3002\u4e3a\u4e86\u89e3\u51b3\u8fd9\u4e2a\u95ee\u9898\uff0c\u78b3\u81ea\u7531\u57fa\u76841,4-\u52a0\u6210\u5f62\u6210C-C\u952e\u7684\u4fee\u9970\u65b9\u6cd5\u88ab\u5f00\u53d1\u4f7f\u7528\uff08\u5177\u4f53\u8bf7\u9605\u8bfb\u6587\u732e5\uff09[5]\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-16962 size-full\" src=\"https:\/\/assets.www.gsbet888.com\/uploads\/2017\/12\/Dha_conjugation_5-e1509693919826.png\" alt=\"\" width=\"607\" height=\"300\" srcset=\"https:\/\/assets.www.gsbet888.com\/uploads\/2017\/12\/Dha_conjugation_5-e1509693919826.png 607w, https:\/\/assets.www.gsbet888.com\/uploads\/2017\/12\/Dha_conjugation_5-e1509693919826-300x148.png 300w\" sizes=\"(max-width: 607px) 100vw, 607px\" \/><\/p>\n<p>\u4f7f\u7528\u91d1\u5c5e\u50ac\u5316\u5242\u8fdb\u884c\u8f6c\u6362\u7684\u4f8b\u5b50\u4e5f\u6709\u5f88\u591a\uff0c\u800c\u5bf9\u4e8e\u5b98\u80fd\u56e2\u517c\u5bb9\u6027\u7684\u95ee\u9898\u4e5f\u662f\u7ecf\u5e38\u53d1\u751f\uff0c\u73b0\u5728\u57fa\u672c\u505c\u7559\u5728\u6c28\u57fa\u9178\u30fb\u591a\u80bd\u7684\u79c0\u65f6\u5c1a\u3002\u800c\u5176\u4e2d\u4e00\u4e2a\u5178\u578b\u7684\u4f8b\u5b50\uff0c\u5982\u4e0b\u56fe\u6240\u793a\u7684\u8fd0\u7528Rh\u50ac\u5316\u5242\u8fdb\u884c\u7684\u82b3\u57fa\u787c\u9178\u76841,4-\u52a0\u6210\u53cd\u5e94[6]\u3002\u6b64\u5916\u4e5f\u80fd\u8f6c\u5316\u6210\u787c\u5316\/\u7532\u7845\u70f7\u57fa\u5316[7]\u3002\u91d1\u5c5e\u9176\uff08p450\uff09\u50ac\u5316\u7684\u73af\u4e19\u70f7\u5316\u4e5f\u6709\u62a5\u9053[8]\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-16963 size-full\" src=\"https:\/\/assets.www.gsbet888.com\/uploads\/2017\/12\/Dha_conjugation_6-e1509693951397.png\" alt=\"\" width=\"660\" height=\"200\" srcset=\"https:\/\/assets.www.gsbet888.com\/uploads\/2017\/12\/Dha_conjugation_6-e1509693951397.png 660w, https:\/\/assets.www.gsbet888.com\/uploads\/2017\/12\/Dha_conjugation_6-e1509693951397-300x91.png 300w\" sizes=\"(max-width: 660px) 100vw, 660px\" \/><\/p>\n<h4>\u53c2\u8003\u6587\u732e<\/h4>\n<ol>\n<li>Repka, L. M.; Chekan, J. R.; Nair, S. K.; van der Donk, W. A. <em>Chem. Rev.<\/em> <strong>2017<\/strong>, <em>117<\/em>, 5457. DOI: <a href=\"http:\/\/dx.doi.org\/10.1021\/acs.chemrev.6b00591\">10.1021\/acs.chemrev.6b00591<\/a><\/li>\n<li>(a) Chalker, J. M.; Gunnoo, S. B.; Boutureira, O.; Gerstberger, S. C.; Fern\u00e1ndez-Gonz\u00e1lez, M.; Bernardes, G. J. L.; Griffin, L.; Hailu, H.; Schofield. C. J.; Davis, B. G. <em>Chem. Sci.<\/em> <strong>2011<\/strong>, <em>2<\/em>, 1666. DOI: <a href=\"http:\/\/dx.doi.org\/10.1039\/c1sc00185j\">10.1039\/c1sc00185j<\/a> (b) Okamoto, R.; Souma, S.; Kajihara, Y. <em>J. Org. Chem.<\/em> <strong>2009<\/strong>, <em>74<\/em>, 2494. DOI: <a href=\"http:\/\/dx.doi.org\/10.1021\/jo8026164\">10.1021\/jo8026164<\/a><\/li>\n<li>Bernardes, G. J. L.; Chalker, J. M.; Errey, J. C.; Davis, B. G. <em>J. Am. Chem. Soc.<\/em><strong>2008<\/strong>, <em>130<\/em>, 5052. DOI: <a href=\"http:\/\/dx.doi.org\/10.1021\/ja800800p\">10.1021\/ja800800p<\/a><\/li>\n<li>Chalker, J. M.; Lercher, L.; Rose, N. R.; Schofield, C. J.; Davis, B. G. <em>Angew. Chem. Int. Ed.<\/em> <strong>2012<\/strong>, <em>51<\/em>, 1835. DOI: <a href=\"http:\/\/dx.doi.org\/10.1002\/anie.201106432\">10.1002\/anie.201106432<\/a><\/li>\n<li>(a) Yang, A.; Ha, S.; Ahn, J.; Kim, R.; Kim, S.; Lee, Y.; Kim, J.; S\u00f6ll, D.; Lee, H.-Y.; Park, H.-S. <em>Science<\/em> <strong>2016<\/strong>, <em>354<\/em>, 623. DOI: <a href=\"http:\/\/dx.doi.org\/10.1126\/science.aah4428\">10.1126\/science.aah4428<\/a> (b) Wright, T. H.; Bower, B. J.; Chalker, J. M.; Bernardes, G. J. J.; Wiewiora, R.; Ng, W.-L. L.; Raj, R.; Faulkner, S.; Vall\u00e9e, M. R.; Phanumartwiwath, A.; Coleman, O. D.; Th\u00e9z\u00e9nas, M.-L. L.; Khan, M.; Galan, S. R. R.; Lercher, L.; Schombs, M. W.; Gerstberger, S.; Palm-Espling, M. E.; Baldwin, A. J.; Kessler, B. M.; Claridge, T. D.; Mohammed, S.; Davis, B. G. <em>Science<\/em> <strong>2016<\/strong>, <em>354<\/em>, aag1465. DOI: <a href=\"http:\/\/dx.doi.org\/10.1126\/science.aag1465\">10.1126\/science.aag1465<\/a><\/li>\n<li>Key,H. M.; Miller, S. J. <em>J. Am. Chem. Soc.<\/em> <strong>2017<\/strong>, DOI: <a href=\"http:\/\/dx.doi.org\/10.1021\/jacs.7b08775\">10.1021\/jacs.7b08775<\/a><\/li>\n<li>Bartoccini, F.; Bartolucci, S.; Lucarini, S.; Piersanti, G. <em>Eur. J. Org. Chem.<\/em> <strong>2015<\/strong>, <em>15<\/em>, 3352. DOI: <a href=\"http:\/\/dx.doi.org\/10.1002\/ejoc.201500362\">10.1002\/ejoc.201500362<\/a><\/li>\n<li>Gober, J. G.; Ghodge, S. V.; Bogart, J. W.; Wever, W. J.; Watkins, R. R.; Brustad, E. M.; Bowers, A. A. <em>ACS Chem. Biol.<\/em> <strong>2017<\/strong>, <em>12<\/em>, 1726. DOI: <a href=\"http:\/\/dx.doi.org\/10.1021\/acschembio.7b00358\">10.1021\/acschembio.7b00358<\/a><\/li>\n<\/ol>\n<h4>\u95a2\u9023\u66f8\u7c4d<\/h4>\n<div class=\"amazonjs_item amazonjs_book\">\n<div class=\"amazonjs_image\"><a class=\"amazonjs_link\" title=\"Bioconjugate Techniques, Third Edition\" href=\"https:\/\/www.amazon.co.jp\/Bioconjugate-Techniques-Third-Greg-Hermanson\/dp\/0123822394?SubscriptionId=AKIAI6Q3RDQBU2J6P3JQ&amp;tag=chemstation-22&amp;linkCode=xm2&amp;camp=2025&amp;creative=165953&amp;creativeASIN=0123822394\" target=\"_blank\" rel=\"noopener\" data-role=\"amazonjs_product\" data-asin=\"0123822394\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/images-fe.ssl-images-amazon.com\/images\/I\/51k5VmvFVlL._SL160_.jpg\" alt=\"Bioconjugate Techniques, Third Edition\" width=\"125\" height=\"160\" \/><\/a><\/div>\n<div class=\"amazonjs_info\">\n<h4><a class=\"amazonjs_link\" title=\"Bioconjugate Techniques, Third Edition\" href=\"https:\/\/www.amazon.co.jp\/Bioconjugate-Techniques-Third-Greg-Hermanson\/dp\/0123822394?SubscriptionId=AKIAI6Q3RDQBU2J6P3JQ&amp;tag=chemstation-22&amp;linkCode=xm2&amp;camp=2025&amp;creative=165953&amp;creativeASIN=0123822394\" target=\"_blank\" rel=\"noopener\" data-role=\"amazonjs_product\" data-asin=\"0123822394\">Bioconjugate Techniques, Third Edition<\/a><\/h4>\n<ul>\n<li><b>\u8457\u8005<\/b>Greg T. Hermanson<\/li>\n<li class=\"amazonjs_price\" title=\"\u4fa1\u683c\u304a\u3088\u3073\u767a\u9001\u53ef\u80fd\u6642\u671f\u306f\u8868\u793a\u3055\u308c\u305f\u65e5\u4ed8\/\u6642\u523b\u306e\u6642\u70b9\u306e\u3082\u306e\u3067\u3042\u308a\u3001\u5909\u66f4\u3055\u308c\u308b\u5834\u5408\u304c\u3042\u308a\u307e\u3059\u3002\u5546\u54c1\u306e\u8ca9\u58f2\u306b\u304a\u3044\u3066\u306f\u3001\u8cfc\u5165\u306e\u6642\u70b9\u3067 [Amazon.co.jp \u307e\u305f\u306f Javari.jp] \u306b\u8868\u793a\u3055\u308c\u3066\u3044\u308b\u4fa1\u683c\u304a\u3088\u3073\u767a\u9001\u53ef\u80fd\u6642\u671f\u306e\u60c5\u5831\u304c\u9069\u7528\u3055\u308c\u307e\u3059\u3002\"><b>\u53c2\u8003\u4fa1\u683c<\/b><span class=\"amazonjs_listprice\">\uffe5 12,249<\/span><br \/>\n<b>\u4fa1\u683c<\/b>\uffe5 12,136(2017\/12\/01 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amazonjs_book\">\n<div class=\"amazonjs_image\"><a class=\"amazonjs_link\" title=\"Bioconjugation Protocols: Strategies and Methods (Methods in Molecular Biology)\" href=\"https:\/\/www.amazon.co.jp\/Bioconjugation-Protocols-Strategies-Methods-Molecular\/dp\/1493960962?SubscriptionId=AKIAI6Q3RDQBU2J6P3JQ&amp;tag=chemstation-22&amp;linkCode=xm2&amp;camp=2025&amp;creative=165953&amp;creativeASIN=1493960962\" target=\"_blank\" rel=\"noopener\" data-role=\"amazonjs_product\" data-asin=\"1493960962\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/images-fe.ssl-images-amazon.com\/images\/I\/41uhsjSN1VL._SL160_.jpg\" alt=\"Bioconjugation Protocols: Strategies and Methods (Methods in Molecular Biology)\" width=\"112\" height=\"160\" \/><\/a><\/div>\n<div class=\"amazonjs_info\">\n<h4><a class=\"amazonjs_link\" title=\"Bioconjugation Protocols: Strategies and Methods (Methods in Molecular Biology)\" href=\"https:\/\/www.amazon.co.jp\/Bioconjugation-Protocols-Strategies-Methods-Molecular\/dp\/1493960962?SubscriptionId=AKIAI6Q3RDQBU2J6P3JQ&amp;tag=chemstation-22&amp;linkCode=xm2&amp;camp=2025&amp;creative=165953&amp;creativeASIN=1493960962\" target=\"_blank\" rel=\"noopener\" data-role=\"amazonjs_product\" data-asin=\"1493960962\">Bioconjugation Protocols: Strategies and Methods (Methods in Molecular Biology)<\/a><\/h4>\n<ul>\n<li class=\"amazonjs_price\" title=\"\u4fa1\u683c\u304a\u3088\u3073\u767a\u9001\u53ef\u80fd\u6642\u671f\u306f\u8868\u793a\u3055\u308c\u305f\u65e5\u4ed8\/\u6642\u523b\u306e\u6642\u70b9\u306e\u3082\u306e\u3067\u3042\u308a\u3001\u5909\u66f4\u3055\u308c\u308b\u5834\u5408\u304c\u3042\u308a\u307e\u3059\u3002\u5546\u54c1\u306e\u8ca9\u58f2\u306b\u304a\u3044\u3066\u306f\u3001\u8cfc\u5165\u306e\u6642\u70b9\u3067 [Amazon.co.jp \u307e\u305f\u306f Javari.jp] \u306b\u8868\u793a\u3055\u308c\u3066\u3044\u308b\u4fa1\u683c\u304a\u3088\u3073\u767a\u9001\u53ef\u80fd\u6642\u671f\u306e\u60c5\u5831\u304c\u9069\u7528\u3055\u308c\u307e\u3059\u3002\"><b>\u53c2\u8003\u4fa1\u683c<\/b><span class=\"amazonjs_listprice\">\uffe5 24,479<\/span><br \/>\n<b>\u4fa1\u683c<\/b>\uffe5 24,204(2017\/12\/02 06:52\u6642\u70b9)<\/li>\n<li><b>\u51fa\u7248\u65e5<\/b>2016\/08\/23<\/li>\n<li><b>\u30da\u30fc\u30d1\u30fc\u30d0\u30c3\u30af<\/b>624\u30da\u30fc\u30b8<\/li>\n<li><b>ISBN-10<\/b>1493960962<\/li>\n<li><b>ISBN-13<\/b>9781493960965<\/li>\n<li><b>\u51fa\u7248\u793e<\/b>Humana Press<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<h4>\u5916\u90e8\u30ea\u30f3\u30af<\/h4>\n<ul>\n<li><a href=\"https:\/\/en.wikipedia.org\/wiki\/Bioconjugation\">Bioconjugation \u2013 Wikipedia<\/a><\/li>\n<li><a href=\"https:\/\/en.wikipedia.org\/wiki\/Dehydroalanine\">Dehydroalanine \u2013 Wikipedia<\/a><\/li>\n<li><a href=\"http:\/\/users.ox.ac.uk\/~dplb0149\/\">The Davis Group<\/a><\/li>\n<\/ul>\n<p>\u672c\u6587\u7248\u6743\u5c5e\u4e8e<strong>\u00a0<a href=\"\/\/www.gsbet888.com\/\">Chem-Station\u5316\u5b66\u7a7a\u95f4<\/a>\uff0c<\/strong> \u6b22\u8fce\u70b9\u51fb\u6309\u94ae\u5206\u4eab\uff0c\u672a\u7ecf\u8bb8\u53ef\uff0c\u8c22\u7edd\u8f6c\u8f7d\uff01 <\/p>\n","protected":false},"excerpt":{"rendered":"\u8131\u6c22\u4e19\u6c28\u9178(dehydroalanine, Dha)\u662f\u7531\u4e1d\u6c28\u9178\u6216\u8005\u534a\u80f1\u9170\u80fa\u8f6c\u5316\u6765\u7684\u7279\u6b8a\u6c28\u57fa\u9178\u3001\u7531\u4e8e\u5176\u5177\u6709Michael\u52a0\u6210acceptor\u7ed3\u6784\uff0c\u6240\u4ee5\u4f5c\u4e3a\u86cb\u767d\u8d28\u7684\u4fee\u9970\u8d77\u70b9\u4e5f\u53d7\u5230\u4e00\u5b9a\u7684\u5173\u6ce8\u3002\u4e5f\u5c31\u662f\uff0c\u5927\u90e8\u5206\u7684\u6c28\u57fa\u9178\u6b8b\u57fa\u5bf9\u4eb2\u6838\u8bd5\u5242\u6216\u8005\u81ea\u7531\u57fa\u663e\u60f0\u6027\u3001\u5229\u7528\u8fd9\u4e00\u7279\u6027\uff0c\u53ef\u4ee5\u9009\u62e9\u6027\u7684\u5bf9\u7ffb\u8bd1\u540e\u7684\u542b\u6709\u8131\u6c22\u4e19\u6c28\u9178\u7684\u86cb\u767d\u8d28\u5176\u8fdb\u884c\u4fee\u9970\u3002 \u57fa\u672c\u6587\u732e Zhu,Y.; van der Donk, W. A. Org. Lett. 2001, 3, 1189. DOI: 10.1021\/ol015648a Galonic, D. P.; van der Donk, W. A.; Gin, D. Y. Chem. Eur. J. 2003, 9, 5997. DOI: 10.1002\/chem.200305290 Levengood, M. R.; van der Donk, W. A. Nat. Protoc. 2007, 1, 3001. doi:10.1038\/nprot.2006.470 Wright, T. H.; Davis, B. G. Nat. Protoc. 2017, 12, 2243. doi:10.1038\/nprot.2017.087 &lt;Installation of Dha&gt; Chalker, J. M.; Gunnoo, S. B.; Boutureira, O.; Gerstberger, S. C.; Fern\u00e1ndez-Gonz\u00e1lez, M.; Bernardes, G. J. L.; Griffin, L.; Hailu, H.; Schofield. C. J.; Davis, B. G. Chem. Sci. 2011, 2, 1666. DOI: 10.1039\/c1sc00185j &lt;Review&gt; Chalker, J. M.; Bernardes, G. J. L.; Lin, Y. A.; Davis, [&hellip;]","protected":false},"author":5,"featured_media":16959,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","enabled":false},"version":2}},"categories":[2],"tags":[1604,2132,59],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"https:\/\/assets.www.gsbet888.com\/uploads\/2017\/12\/Dha_conjugation_0.png","jetpack_shortlink":"https:\/\/wp.me\/p96op1-4pw","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/posts\/16958"}],"collection":[{"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/users\/5"}],"replies":[{"embeddable":true,"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/comments?post=16958"}],"version-history":[{"count":8,"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/posts\/16958\/revisions"}],"predecessor-version":[{"id":27238,"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/posts\/16958\/revisions\/27238"}],"wp:featuredmedia":[{"embeddable":true,"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/media\/16959"}],"wp:attachment":[{"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/media?parent=16958"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/categories?post=16958"},{"taxonomy":"post_tag","embeddable":true,"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/tags?post=16958"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}