{"id":17002,"date":"2017-12-05T06:18:39","date_gmt":"2017-12-04T22:18:39","guid":{"rendered":"\/\/www.gsbet888.com\/?p=17002"},"modified":"2019-08-02T18:52:17","modified_gmt":"2019-08-02T10:52:17","slug":"%e7%a3%81%e6%80%a7%e7%ba%b3%e7%b1%b3%e7%b2%92%e5%ad%90%e5%af%b9%e7%99%8c%e7%bb%86%e8%83%9e%e7%9a%84%e7%ad%9b%e9%80%89","status":"publish","type":"post","link":"\/\/www.gsbet888.com\/%e5%8c%96%e5%ad%a6%e6%9d%82%e8%ae%b0\/recentpaper\/2017\/12\/%e7%a3%81%e6%80%a7%e7%ba%b3%e7%b1%b3%e7%b2%92%e5%ad%90%e5%af%b9%e7%99%8c%e7%bb%86%e8%83%9e%e7%9a%84%e7%ad%9b%e9%80%89.html","title":{"rendered":"\u78c1\u6027\u7eb3\u7c73\u7c92\u5b50\u5bf9\u764c\u7ec6\u80de\u7684\u7b5b\u9009"},"content":{"rendered":"

\u672c\u6587\u7ffb\u8bd1\u6295\u7a3f\u5f20\u5bfb<\/strong><\/p>\n

\u6700\u8fd1\uff0c\u6211\u5728\u5b66\u6821\u542c\u4e86\u4e00\u6b21\u8ba9\u6211\u5370\u8c61\u9887\u6df1\u7684\u6f14\u8bb2\uff0c\u4e8e\u662f\u60f3\u5728\u8fd9\u91cc\u5411\u5927\u5bb6\u5206\u4eab\u3002<\/p>\n

\u672c\u6b21\u7684\u6f14\u8bb2\u8005\u662f\u591a\u4f26\u591a\u5927\u5b66\u7684Shana O. Kelley<\/a>\u6559\u6388\u3002\u5979\u81f4\u529b\u4e8e\u4ee5DNA\u4e3a\u6a21\u677f\u7684\u7eb3\u7c73\u6750\u6599\u548c\u68c0\u6d4b\u7279\u5b9a\u7ec6\u80de\u3001\u751f\u7269\u5206\u5b50\u7684\u751f\u7269\u88c5\u7f6e\u4e3a\u4e2d\u5fc3\u7684\u7814\u7a76\u3002\u540c\u65f6\u5979\u7684\u540d\u5b57\u4e5f\u66fe\u88ab\u5217\u5165MIT\u2019s Technology Review<\/a>\u7684top100\u521b\u65b0\u7814\u7a76\u8005\uff082004\uff09\u4e4b\u4e2d\u3002<\/p>\n

\"\"<\/a><\/p>\n

Shana O. Kelley\u6559\u6388\uff08\u7167\u7247\u6765\u6e90\uff1aKelly\u7814\u7a76\u5ba4\u7f51\u7ad9\u4e3b\u9875<\/a>\uff09<\/p>\n

\u4ee5\u4e0b\u662f\u672c\u6587\u6240\u5f15\u7528\u7684\u8bba\u6587\u3002<\/p>\n

Tracking the dynamics of circulating tumour cell phenotypes using nanoparticle-mediated magnetic ranking\u201d<\/p>\n

Poudineh, M.; Aldridge, P. M.; Ahmed, S.; Green, B. J.; Kermanshah, L.; Nguyen, V.; Tu, C.; Mohamadi, R. M.; Nam, R. K.; Hansen, A.; Sridhar, S. S.; Finelli, A.; Fleshner, N. E.; Joshua, A. M.; Sargent, E. H.; Kelley, S. O.<\/p>\n

Nat. Nanotechnol.<\/em>2017,<\/strong>12<\/em>, 274. DOI<\/p><\/blockquote>\n

\uff11<\/strong>. <\/strong>\u7814\u7a76\u80cc\u666f<\/strong><\/h4>\n

\"\"<\/a><\/p>\n

\u672c\u6b21\u7814\u7a76\u7684\u5bf9\u8c61\u4e3a\u4ece\u764c\u53d8\u7684\u7ec4\u7ec7\u4e2d\u8131\u79bb\uff0c\u5728\u8840\u6db2\u4e2d\u5faa\u73af\u7684\u80bf\u7624\uff08\u764c\uff09\u7ec6\u80de\u3002<\/p>\n

\u764c\u7ec6\u80de\u4ece\u7ec4\u7ec7\u8131\u79bb\u8fdb\u5165\u8840\u7ba1\u3002\u867d\u7136\u5927\u591a\u6570\u764c\u7ec6\u80de\u4f1a\u56e0\u4eba\u4f53\u81ea\u8eab\u514d\u75ab\u7cfb\u7edf\u653b\u51fb\u800c\u88ab\u6740\u6b7b\uff0c\u4f46\u5c11\u6570\u7684\u764c\u7ec6\u80de\u4f1a\u5b58\u6d3b\uff0c\u5728\u8840\u6db2\u4e2d\u7684\u6d53\u5ea6\u4e3a1ppb(\u5341\u4ebf\u5206\u4e4b\u4e00)\u5de6\u53f3\u3002\u76ee\u524d\u666e\u904d\u8ba4\u4e3a\uff0c\u764c\u7ec6\u80de\u8f6c\u79fb\u4e0eCTC(\u5faa\u73af\u80bf\u7624\u7ec6\u80de)\u6709\u5173\u3002\u5bf9CTC\u4e0a\u53d1\u73b0\u7684\u751f\u7269\u6807\u5fd7\u7269\u53ca\u5176\u9057\u4f20\u57fa\u56e0\u53d8\u5f02\u7b49\u65b9\u9762\u7684\u7814\u7a76\u76ee\u524d\u5341\u5206\u70ed\u95e8<\/p>\n

\u4f46\u56e0\u4e3aCTC\u5728\u6bcf10ml\u8840\u6db2\u4e2d\u53ea\u5b58\u5728\u51e0\u4e2a\u6216\u51e0\u5341\u4e2a\uff0c\u5176\u4ed6\u7684\u8840\u6db2\u7ec6\u80de\u542b\u91cf\u76f8\u5bf9\u8981\u591a\u5f88\u591a\u3002\u6240\u4ee5\u4ece\u8840\u6db2\u6837\u672c\u4e2d\u6709\u9009\u62e9\u6027\u5730\u63d0\u53d6CTC\u662f\u5341\u5206\u56f0\u96be\u7684\u3002\u5728\u751f\u7269\u5b66\u9886\u57df\u4e2d\uff0c\u4f7f\u7528\u8367\u5149\u6807\u8bc6\u6765\u7b5b\u9009\u7ec6\u80de\u7684FAC\u6cd5(fluorescence activated cell sorting)\u88ab\u5e7f\u6cdb\u4f7f\u7528\u3002\u4f46\u76ee\u524d\u7684\u7075\u654f\u5ea6\u548c\u5206\u8fa8\u7387\u8fd8\u96be\u4ee5\u7b5b\u9009\u8840\u6db2\u4e2d\u7684CTC\u3002<\/p>\n

2<\/strong>\u3001\u901a\u8fc7\u78c1\u6027\u7eb3\u7c73\u7c92\u5b50\u6765\u6355\u6349\u7ec6\u80de<\/strong><\/h4>\n

\"\"<\/a><\/p>\n

\u56fe<\/strong>2\u00a0\u00a0\u00a0\u00a0 <\/strong>\u901a\u8fc7\u78c1\u6027\u7b5b\u9009\u7ec6\u80de\u7684\u8bbe\u5907<\/strong><\/p>\n

 <\/p>\n

\u8be5\u65b9\u6cd5\u662f\u901a\u8fc7\u4ee5\u4e0b\u9014\u5f84\u6765\u5b9e\u73b0\u7684\uff1a<\/strong><\/p>\n

1\u3001\u4f7f\u7528\u5e26\u6297\u4f53\u7684\u78c1\u6027\u7eb3\u7c73\u7c92\u5b50\u6765\u6807\u8bb0\u8840\u6db2\u4e2d\u7684\u5e72\u7ec6\u80de<\/p>\n

2\u3001\u4f7f\u8840\u6db2\u6837\u672c\u6d41\u8fc7\u5fae\u89c2\u6d41\u4f53\u8bbe\u5907\uff08\u56fe2\uff09<\/p>\n

3\u3001\u901a\u8fc7\u6d41\u8def\u4e2d\u7684X\u578b\u7ed3\u6784\uff08\u56fe2\u4e2d\u9ec4\u8272\u90e8\u5206\uff09\uff0c\u7b5b\u9009\u5e76\u6355\u6349\u76ee\u6807\u7ec6\u80de\u3002<\/p>\n

\u672c\u65b9\u6cd5\u7684\u5173\u952e\u5728\u4e8e\u5fae\u89c2\u6d41\u4f53\u8bbe\u5907\u5185\u8bbe\u7f6e\u7684X\u7ed3\u6784\u3002X\u7ed3\u6784\u7684\u4e2d\u5fc3\u653e\u5165\u4e86\u954d\u5fae\u78c1\u4f53\uff0c\u4ece\u800c\u53ef\u4ee5\u4e0e\u7ec6\u80de\u4e0a\u7684\u78c1\u6027\u7eb3\u7c73\u7c92\u5b50\u76f8\u4e92\u5438\u5f15\u3002\u5fae\u78c1\u4f53\u7684\u5927\u5c0f\uff0c\u4ece\u6d41\u8def\u5165\u53e3\u5230\u51fa\u53e3\u6e10\u6e10\u53d8\u5927\uff0c\u8fdb\u800c\u80fd\u591f\u63a7\u5236\u7ec6\u80de\u548c\u6d41\u8def\u4e4b\u95f4\u7684\u76f8\u4e92\u4f5c\u7528\u3002<\/p>\n

\"\"<\/a><\/p>\n

\u56fe<\/strong>3\u00a0\u00a0 <\/strong>\u7b5b\u9009\u7ec6\u80de\u7684\u5fae\u6d41\u4f53\u8bbe\u5907\u7684\u5185\u90e8\u6784\u9020<\/strong><\/p>\n

\u5728\u7ec6\u80de\u8868\u9762\u4e0a\u6807\u5fd7\u7684\u78c1\u6027\u7eb3\u7c73\u7c92\u5b50\u6570\u91cf\u591a\u7684\u65f6\u5019\uff0c\u4e3a\u4f7f\u954d\u5fae\u78c1\u4f53\u548c\u7ec6\u80de\u95f4\u7684\u76f8\u4e92\u4f5c\u7528\u589e\u5f3a\uff0c\u5982\u56fe3\u4e2d\u7684\uff08i\uff09\uff0c\u4e00\u822c\u4f1a\u6269\u5927\u5176\u6355\u6349\u8303\u56f4\u3002\u76f8\u53cd\uff0c\u5982\u679c\u7ec6\u80de\u4e0a\u6807\u5fd7\u7684\u78c1\u6027\u7eb3\u7c73\u7c92\u5b50\u6570\u91cf\u5c11\u7684\u65f6\u5019\uff0c\u5c31\u4f1a\u50cf\u56fe3\u4e2d\u7684\uff08ii\uff09\u4e00\u6837\u5c06\u6355\u6349\u9886\u57df\u7f29\u5c0f\u3002\u901a\u8fc7\u8fd9\u6837\u7684\u539f\u7406\uff0c\u78c1\u6027\u7c92\u5b50\u88c5\u8f7d\u91cf\u4e0d\u540c\u7684\u7ec6\u80de\u5c31\u53ef\u4ee5\u901a\u8fc7\u5fae\u6d41\u4f53\u8bbe\u5907\u8fdb\u884c\u5206\u79bb\u3002<\/p>\n

\u5173\u4e8e\u78c1\u6027\u7eb3\u7c73\u7c92\u5b50\u7684\u88c5\u8f7d\u91cf\uff0c\u56e0\u4e3a\u5728\u7ec6\u80de\u4e0a\u53d1\u73b0\u7684\u751f\u7269\u6807\u5fd7\u7269\u6570\u91cf\u4e0d\u540c\uff0c\u5c31\u53ef\u4ee5\u901a\u8fc7\u7ec6\u80de\u8868\u9762\u7279\u6027\u7684\u4e0d\u540c\u800c\u5bf9\u7ec6\u80de\u8fdb\u884c\u7b5b\u9009\u3002<\/p>\n

3<\/strong>\u3001\u6839\u636e\u4e0a\u76ae\u7ec6\u80de\u7c98\u9644\u5206\u5b50\uff08<\/strong>EpCAM<\/strong>\uff09\u7684\u6570\u91cf\u6765\u7b5b\u9009\u764c\u7ec6\u80de<\/strong><\/h4>\n

Kelley\u7b49\u9996\u5148\u5c1d\u8bd5\u4e86\u8fd9\u79cd\u65b9\u5f0f\uff0c\u5e76\u4e14\u901a\u8fc7\u76f8\u5e94\u7684\u5b9e\u9a8c\u6765\u9a8c\u8bc1\u662f\u5426\u5176\u5177\u6709\u7b5b\u9009\u4e0d\u540c\u764c\u7ec6\u80de\u7684\u80fd\u529b\u3002\u5b9e\u9a8c\u4e2d\u4f7f\u7528MCF-7\u3001SKBR3\u3001PC-3\u3001MDA-MB-231\u56db\u79cd\u7ec6\u80de\u4e3a\u5bf9\u8c61\u3002\u4ed4\u7ec6\u8c03\u67e5\u8fd9\u4e9b\u7ec6\u80de\u7684\u4e0a\u76ae\u7ec6\u80de\u7c98\u9644\u5206\u5b50\u6570\u91cf\uff0c\u5e76\u4ee5\u6b64\u4e3a\u6807\u5fd7\u6765\u8fdb\u884c\u7ec6\u80de\u7b5b\u9009\uff08\u56fe4\uff09<\/p>\n

\"\"<\/a><\/p>\n

\u56fe<\/strong>4<\/strong>\u6839\u636e<\/strong>EpCAM<\/strong>\u7684\u68c0\u51fa\u91cf\u4e0d\u540c\u5bf9\u4e8e\u764c\u7ec6\u80de\u8fdb\u884c\u7b5b\u9009\u3002\u56fe\u4e2d\u6240\u793a\u4e3a\u901a\u8fc7\u6d41\u5f0f\u7ec6\u80de\u4eea\u7b5b\u9009\u7684\u6570\u636e\u3002<\/strong><\/p>\n

\u8ba9\u7ec6\u80de\u5206\u522b\u6d41\u7ecfMagRC\uff0c\u56db\u79cd\u7ec6\u80de\u5c06\u4f1a\u5206\u5e03\u5728\u8bbe\u5907\u4e2d\u7684\u4e0d\u540c\u533a\u57df\u3002\u56e0\u4e3aMCF-7\u5728\u79bb\u6d41\u8def\u5165\u53e3\u6700\u8fd1\u7684\u4f4d\u7f6e\uff08Zone number\u6bd4\u8f83\u5c0f\uff0c\u4e14\u954d\u78c1\u4f53\u6bd4\u8f83\u5c0f\u7684\u5730\u65b9\uff09\u88ab\u6355\u6349\u5230\uff0c\u6240\u4ee5\u4e5f\u5c31\u8868\u660eEpCAM\u91cf\u8f83\u5927\u3002\u53e6\u4e00\u65b9\u9762\uff0c\u6211\u4eec\u5728\u51fa\u53e3\u9644\u8fd1\u6355\u6349\u5230\u4e86PC-3\u548cMDA-MB-231\uff0c\u8fd9\u8bf4\u660e\u5b83\u4eec\u7684EpCAM\u91cf\u5f88\u5c11\u3002\u5b9e\u9645\u4e0a\uff0c\u5982\u679c\u901a\u8fc7\u6d41\u5f0f\u7ec6\u80de\u6cd5\u7b5b\u9009\u8fd9\u56db\u79cd\u7ec6\u80de\u7684\u8bdd\uff0c\u4e0d\u96be\u7406\u89e3\u5176\u68c0\u51faEpCAM\u7684\u91cf\u662f\u548cMagRC\u7684\u68c0\u6d4b\u7ed3\u679c\u4e00\u81f4\u7684\u3002\u7279\u522b\u9700\u8981\u6ce8\u610f\u7684\u662f\uff0c\u548c\u6d41\u5f0f\u7ec6\u80de\u6cd5\u76f8\u6bd4\uff0c\u4f7f\u7528MagRC<\/u><\/strong>\u6cd5\uff0c\u89e3\u6790\u80fd\u529b\u4f1a\u66f4\u52a0\u51fa\u8272\u3002<\/u><\/strong><\/p>\n

4. \u8840\u6db2\u6837\u54c1\u4e2d\u764c\u7ec6\u80de\u7684\u6355\u6349<\/h4>\n

\u7531\u4e0a\u8ff0\u53ef\u77e5\uff0c\u5b9e\u9a8c\u6240\u7528\u7684\u6837\u672c\u53ea\u542b\u6709\u5355\u4e00\u79cd\u7c7b\u7684\u7ec6\u80de\uff0c\u5982\u679c\u8981\u8fd0\u7528\u4e8e\u5b9e\u9645\u4e2d\uff0c\u5219\u5c31\u5fc5\u987b\u8981\u80fd\u5728\u6df7\u6742\u7740\u7ea2\u7ec6\u80de\u548c\u767d\u7ec6\u80de\u7684\u8840\u6db2\u4e2d\u5206\u79bb\u51fa\u764c\u7ec6\u80de\u3002\u56e0\u6b64\uff0cKelly\u7b49\u4eba\u4f7f\u7528\u4eba\u4f53\u5168\u8840\uff0c\u4ee5EpCAM\u4e3a\u6807\u5fd7\u7269\u5bf9\u4e8e\u7ec6\u80de\u8fdb\u884c\u7b5b\u9009\u3002<\/p>\n

\"\"<\/a><\/p>\n

\u56fe<\/strong>5 PBS<\/strong>\uff08\u4e0a\u65b9\u4e24\u56fe\uff09\u4ee5\u53ca\u5168\u8840\uff08\u4e0b\u65b9\u4e24\u56fe\uff09\u4e2d\u7684\u764c\u7ec6\u80de\u5206\u79bb\u3002\u5de6\u4fa7\u56fe\u4e3a<\/strong>MagRC<\/strong>\u4e2d\u6570\u636e\uff0c\u53f3\u4fa7\u56fe\u4e3a\u6d41\u5f0f\u7ec6\u80de\u4eea\u4e2d\u6570\u636e\u3002<\/strong><\/p>\n

\u56fe5\u5c55\u793a\u4e86\u57281ml\u5168\u8840\uff08\u6216\u8005PBS\uff09\u4e2d\u6df7\u5165100\u4e2a\u621610000\u4e2a\u764c\u7ec6\u80de\uff0c\u4f7f\u7528MagRC\u548c\u6d41\u5f0f\u7ec6\u80de\u4eea\u8fdb\u884c\u7ec6\u80de\u5206\u79bb\u65f6\u7684\u60c5\u51b5\u3002\u5728\u4f7f\u7528\u6d41\u5f0f\u7ec6\u80de\u4eea\u7684PBS\u6837\u672c\u4e2d\uff0c\u764c\u7ec6\u80de\u6570\u91cf\u5c11\u7684\u65f6\u5019\uff08100\u4e2a\uff0c\u5982\u53f3\u4e0a\u56fe\u6240\u793a\uff09\uff0c\u65e0\u6cd5\u5f97\u5230\u76f8\u5e94\u7684\u4fe1\u53f7\u3002\u5728\u5168\u8840\u6837\u672c\u4e2d\u4e5f\u4ec5\u80fd\u5f97\u5230\u672c\u5e95\u4fe1\u53f7\uff0c\u65e0\u6cd5\u68c0\u6d4b\u51fa\u764c\u7ec6\u80de\u3002<\/p>\n

\u53e6\u4e00\u65b9\u9762\uff0c\u5728\u4f7f\u7528MagRC\u65f6\uff0c\u5373\u4f7f\u662f1ml\u4e2d\u53ea\u6df7\u5165100\u4e2a\u764c\u7ec6\u80de\u7684\u6837\u54c1\uff0c\u4e5f\u53ef\u4ee5\u505a\u5230\u6709\u6548\u5730\u5206\u79bb\u3002\u5728\u56fe5\u7684\u5de6\u4fa7\u4e0a\u4e0b\u4e24\u5e45\u56fe\u4e2d\uff0cPBS\u548c\u5168\u8840\u4e2d\u5747\u68c0\u51fa\u7ec6\u80de\uff0c\u7ecf\u786e\u8ba4\uff0c\u68c0\u51fa\u7684\u7ec6\u80de\u4e2d\u5e76\u6ca1\u6709\u9664\u764c\u7ec6\u80de\u4ee5\u5916\u7684\u7ec6\u80de\u3002<\/p>\n

5\u3001\u764c\u75c7\u60a3\u8005\u7684\u8840\u6db2\u5206\u6790<\/strong><\/h4>\n

\u540c\u65f6\uff0cKelley\u7b49\u4e5f\u4f7f\u7528MagRC\u7684\u65b9\u6cd5\u5bf9\u4e8e\u764c\u75c7\u60a3\u8005\u7684\u8840\u6db2\u6837\u672c\u8fdb\u884c\u5206\u6790\u3002\u4ece\u8f6c\u79fb\u6027\u53bb\u52bf\u62b5\u6297\u6027\u524d\u5217\u817a\u764c\uff08mCRPC\uff09\u4ee5\u53ca\u5c40\u9650\u6027\u524d\u5217\u817a\u764c\uff08localized prostate cancer\uff09\u60a3\u8005\u4e2d\u91c7\u96c6\u8840\u6db2\u6837\u672c\uff0c\u4f7f\u7528\u6307\u793a\u764c\u7ec6\u80de\u6076\u5316\u7a0b\u5ea6Gleason\u5206\u7ea7\uff08P1\u5230P14\uff09\u65b9\u6cd5\uff0c\u7ed8\u5236\u4e0b\u56fe\uff08\u56fe6\uff09<\/p>\n

\"\"<\/a><\/p>\n

\u56fe6 \u5bf9\u4e0d\u540c\u79cd\u7c7b\u7684\u764c\u7ec6\u80deCTC\u5206\u5e03\u8fdb\u884c\u6bd4\u8f83\uff08\u6765\u81ea\u4e8e\u8bba\u6587\uff09\uff0c\u524d\u5217\u817a\u764c\uff08\u53f3\u56fe\uff09\u4e2d\uff0cGleason\u5206\u7ea7\u4e3aP1-3: 6\u3001P4-9: 7\u3001P10-14: 8-9\u3002<\/p>\n

\u901a\u8fc7\u6bd4\u8f83\u5de6\u53f3\u4e24\u56fe\u53ef\u4ee5\u770b\u51fa\uff0c\u6bcf\u4e2amCRPC\u60a3\u8005\uff08\u5de6\uff09\u7684EpCAM\u68c0\u51fa\u91cf\u90fd\u5f88\u4f4e\uff0c\u800c\u5c40\u9650\u6027\u524d\u5217\u817a\u764c\u60a3\u8005\uff08\u53f3\uff09\u5219\u56e0\u4e2a\u4f53\u7684\u4e0d\u540c\uff0c\u68c0\u51fa\u91cf\u4e5f\u4e0d\u5c3d\u76f8\u540c\u3002\u968f\u7740Gleason\u5206\u7ea7\u7684\u589e\u5927\uff0cCTC\u7684EpCAM\u53d1\u73b0\u91cf\u9010\u6e10\u51cf\u5c11\u3002\u5982\u679c\u662f\u5177\u6709\u8f6c\u79fb\u6027\u7684\u764c\u75c7\uff0c\u7ec6\u80de\u4e0a\u76ae\u5931\u53bb\u6d3b\u6027\uff0c\u53d1\u751f\u4e0a\u76ae-\u95f4\u53f6\u8f6c\u5316\u65f6\uff0cEpCAM\u7684\u68c0\u51fa\u91cf\u4e5f\u4f1a\u51cf\u5c11\u3002<\/p>\n

6\u3001\u603b\u7ed3<\/strong><\/h4>\n

\u672c\u8bba\u6587\u6240\u4ecb\u7ecd\u7684\u78c1\u6027\u7eb3\u7c73\u79bb\u5b50\u7ec6\u80de\u7b5b\u9009\u6cd5\uff08MagRC\uff09\uff0c\u901a\u8fc7\u7ec6\u80de\u7684\u8868\u9762\u7279\u6027\uff0c\u6210\u529f\u5206\u79bb\u4e86\u8840\u6db2\u4e2d\u6781\u5c11\u91cf\u7684\u764c\u7ec6\u80de\u3002\u6b64\u65b9\u6cd5\u4e0d\u4ec5\u4ec5\u5c40\u9650\u4e8e\u4f7f\u7528EpCAM\u4f5c\u4e3a\u6807\u5fd7\u7269\uff0c\u5176\u4ed6\u5404\u79cd\u6807\u5fd7\u5206\u5b50\u5747\u53ef\u4ee5\u4f7f\u7528\u3002\u5728\u8fd9\u91cc\uff0c\u7b14\u8005\u4e5f\u671f\u5f85\u6b64\u65b9\u6cd5\u4eca\u540e\u80fd\u5bf9\u764c\u7ec6\u80de\u8f6c\u79fb\u7684\u7814\u7a76\u505a\u51fa\u7a81\u51fa\u7684\u8d21\u732e\u3002<\/p>\n

 <\/p>\n

\u672c\u6587\u7248\u6743\u5c5e\u4e8e\u00a0Chem-Station\u5316\u5b66\u7a7a\u95f4<\/a>\uff0c<\/strong> \u6b22\u8fce\u70b9\u51fb\u6309\u94ae\u5206\u4eab\uff0c\u672a\u7ecf\u8bb8\u53ef\uff0c\u8c22\u7edd\u8f6c\u8f7d\uff01 <\/p>\n","protected":false},"excerpt":{"rendered":"\u672c\u6587\u7ffb\u8bd1\u6295\u7a3f\u5f20\u5bfb \u6700\u8fd1\uff0c\u6211\u5728\u5b66\u6821\u542c\u4e86\u4e00\u6b21\u8ba9\u6211\u5370\u8c61\u9887\u6df1\u7684\u6f14\u8bb2\uff0c\u4e8e\u662f\u60f3\u5728\u8fd9\u91cc\u5411\u5927\u5bb6\u5206\u4eab\u3002 \u672c\u6b21\u7684\u6f14\u8bb2\u8005\u662f\u591a\u4f26\u591a\u5927\u5b66\u7684Shana O. Kelley\u6559\u6388\u3002\u5979\u81f4\u529b\u4e8e\u4ee5DNA\u4e3a\u6a21\u677f\u7684\u7eb3\u7c73\u6750\u6599\u548c\u68c0\u6d4b\u7279\u5b9a\u7ec6\u80de\u3001\u751f\u7269\u5206\u5b50\u7684\u751f\u7269\u88c5\u7f6e\u4e3a\u4e2d\u5fc3\u7684\u7814\u7a76\u3002\u540c\u65f6\u5979\u7684\u540d\u5b57\u4e5f\u66fe\u88ab\u5217\u5165MIT\u2019s Technology Review\u7684top100\u521b\u65b0\u7814\u7a76\u8005\uff082004\uff09\u4e4b\u4e2d\u3002 Shana O. Kelley\u6559\u6388\uff08\u7167\u7247\u6765\u6e90\uff1aKelly\u7814\u7a76\u5ba4\u7f51\u7ad9\u4e3b\u9875\uff09 \u4ee5\u4e0b\u662f\u672c\u6587\u6240\u5f15\u7528\u7684\u8bba\u6587\u3002 Tracking the dynamics of circulating tumour cell phenotypes using nanoparticle-mediated magnetic ranking\u201d Poudineh, M.; Aldridge, P. M.; Ahmed, S.; Green, B. J.; Kermanshah, L.; Nguyen, V.; Tu, C.; Mohamadi, R. M.; Nam, R. K.; Hansen, A.; Sridhar, S. S.; Finelli, A.; Fleshner, N. E.; Joshua, A. M.; Sargent, E. H.; Kelley, S. O. Nat. Nanotechnol.2017,12, 274. DOI \uff11. \u7814\u7a76\u80cc\u666f \u672c\u6b21\u7814\u7a76\u7684\u5bf9\u8c61\u4e3a\u4ece\u764c\u53d8\u7684\u7ec4\u7ec7\u4e2d\u8131\u79bb\uff0c\u5728\u8840\u6db2\u4e2d\u5faa\u73af\u7684\u80bf\u7624\uff08\u764c\uff09\u7ec6\u80de\u3002 \u764c\u7ec6\u80de\u4ece\u7ec4\u7ec7\u8131\u79bb\u8fdb\u5165\u8840\u7ba1\u3002\u867d\u7136\u5927\u591a\u6570\u764c\u7ec6\u80de\u4f1a\u56e0\u4eba\u4f53\u81ea\u8eab\u514d\u75ab\u7cfb\u7edf\u653b\u51fb\u800c\u88ab\u6740\u6b7b\uff0c\u4f46\u5c11\u6570\u7684\u764c\u7ec6\u80de\u4f1a\u5b58\u6d3b\uff0c\u5728\u8840\u6db2\u4e2d\u7684\u6d53\u5ea6\u4e3a1ppb(\u5341\u4ebf\u5206\u4e4b\u4e00)\u5de6\u53f3\u3002\u76ee\u524d\u666e\u904d\u8ba4\u4e3a\uff0c\u764c\u7ec6\u80de\u8f6c\u79fb\u4e0eCTC(\u5faa\u73af\u80bf\u7624\u7ec6\u80de)\u6709\u5173\u3002\u5bf9CTC\u4e0a\u53d1\u73b0\u7684\u751f\u7269\u6807\u5fd7\u7269\u53ca\u5176\u9057\u4f20\u57fa\u56e0\u53d8\u5f02\u7b49\u65b9\u9762\u7684\u7814\u7a76\u76ee\u524d\u5341\u5206\u70ed\u95e8 \u4f46\u56e0\u4e3aCTC\u5728\u6bcf10ml\u8840\u6db2\u4e2d\u53ea\u5b58\u5728\u51e0\u4e2a\u6216\u51e0\u5341\u4e2a\uff0c\u5176\u4ed6\u7684\u8840\u6db2\u7ec6\u80de\u542b\u91cf\u76f8\u5bf9\u8981\u591a\u5f88\u591a\u3002\u6240\u4ee5\u4ece\u8840\u6db2\u6837\u672c\u4e2d\u6709\u9009\u62e9\u6027\u5730\u63d0\u53d6CTC\u662f\u5341\u5206\u56f0\u96be\u7684\u3002\u5728\u751f\u7269\u5b66\u9886\u57df\u4e2d\uff0c\u4f7f\u7528\u8367\u5149\u6807\u8bc6\u6765\u7b5b\u9009\u7ec6\u80de\u7684FAC\u6cd5(fluorescence activated cell sorting)\u88ab\u5e7f\u6cdb\u4f7f\u7528\u3002\u4f46\u76ee\u524d\u7684\u7075\u654f\u5ea6\u548c\u5206\u8fa8\u7387\u8fd8\u96be\u4ee5\u7b5b\u9009\u8840\u6db2\u4e2d\u7684CTC\u3002 2\u3001\u901a\u8fc7\u78c1\u6027\u7eb3\u7c73\u7c92\u5b50\u6765\u6355\u6349\u7ec6\u80de \u56fe2\u00a0\u00a0\u00a0\u00a0 \u901a\u8fc7\u78c1\u6027\u7b5b\u9009\u7ec6\u80de\u7684\u8bbe\u5907   \u8be5\u65b9\u6cd5\u662f\u901a\u8fc7\u4ee5\u4e0b\u9014\u5f84\u6765\u5b9e\u73b0\u7684\uff1a 1\u3001\u4f7f\u7528\u5e26\u6297\u4f53\u7684\u78c1\u6027\u7eb3\u7c73\u7c92\u5b50\u6765\u6807\u8bb0\u8840\u6db2\u4e2d\u7684\u5e72\u7ec6\u80de 2\u3001\u4f7f\u8840\u6db2\u6837\u672c\u6d41\u8fc7\u5fae\u89c2\u6d41\u4f53\u8bbe\u5907\uff08\u56fe2\uff09 3\u3001\u901a\u8fc7\u6d41\u8def\u4e2d\u7684X\u578b\u7ed3\u6784\uff08\u56fe2\u4e2d\u9ec4\u8272\u90e8\u5206\uff09\uff0c\u7b5b\u9009\u5e76\u6355\u6349\u76ee\u6807\u7ec6\u80de\u3002 \u672c\u65b9\u6cd5\u7684\u5173\u952e\u5728\u4e8e\u5fae\u89c2\u6d41\u4f53\u8bbe\u5907\u5185\u8bbe\u7f6e\u7684X\u7ed3\u6784\u3002X\u7ed3\u6784\u7684\u4e2d\u5fc3\u653e\u5165\u4e86\u954d\u5fae\u78c1\u4f53\uff0c\u4ece\u800c\u53ef\u4ee5\u4e0e\u7ec6\u80de\u4e0a\u7684\u78c1\u6027\u7eb3\u7c73\u7c92\u5b50\u76f8\u4e92\u5438\u5f15\u3002\u5fae\u78c1\u4f53\u7684\u5927\u5c0f\uff0c\u4ece\u6d41\u8def\u5165\u53e3\u5230\u51fa\u53e3\u6e10\u6e10\u53d8\u5927\uff0c\u8fdb\u800c\u80fd\u591f\u63a7\u5236\u7ec6\u80de\u548c\u6d41\u8def\u4e4b\u95f4\u7684\u76f8\u4e92\u4f5c\u7528\u3002 \u56fe3\u00a0\u00a0 \u7b5b\u9009\u7ec6\u80de\u7684\u5fae\u6d41\u4f53\u8bbe\u5907\u7684\u5185\u90e8\u6784\u9020 \u5728\u7ec6\u80de\u8868\u9762\u4e0a\u6807\u5fd7\u7684\u78c1\u6027\u7eb3\u7c73\u7c92\u5b50\u6570\u91cf\u591a\u7684\u65f6\u5019\uff0c\u4e3a\u4f7f\u954d\u5fae\u78c1\u4f53\u548c\u7ec6\u80de\u95f4\u7684\u76f8\u4e92\u4f5c\u7528\u589e\u5f3a\uff0c\u5982\u56fe3\u4e2d\u7684\uff08i\uff09\uff0c\u4e00\u822c\u4f1a\u6269\u5927\u5176\u6355\u6349\u8303\u56f4\u3002\u76f8\u53cd\uff0c\u5982\u679c\u7ec6\u80de\u4e0a\u6807\u5fd7\u7684\u78c1\u6027\u7eb3\u7c73\u7c92\u5b50\u6570\u91cf\u5c11\u7684\u65f6\u5019\uff0c\u5c31\u4f1a\u50cf\u56fe3\u4e2d\u7684\uff08ii\uff09\u4e00\u6837\u5c06\u6355\u6349\u9886\u57df\u7f29\u5c0f\u3002\u901a\u8fc7\u8fd9\u6837\u7684\u539f\u7406\uff0c\u78c1\u6027\u7c92\u5b50\u88c5\u8f7d\u91cf\u4e0d\u540c\u7684\u7ec6\u80de\u5c31\u53ef\u4ee5\u901a\u8fc7\u5fae\u6d41\u4f53\u8bbe\u5907\u8fdb\u884c\u5206\u79bb\u3002 \u5173\u4e8e\u78c1\u6027\u7eb3\u7c73\u7c92\u5b50\u7684\u88c5\u8f7d\u91cf\uff0c\u56e0\u4e3a\u5728\u7ec6\u80de\u4e0a\u53d1\u73b0\u7684\u751f\u7269\u6807\u5fd7\u7269\u6570\u91cf\u4e0d\u540c\uff0c\u5c31\u53ef\u4ee5\u901a\u8fc7\u7ec6\u80de\u8868\u9762\u7279\u6027\u7684\u4e0d\u540c\u800c\u5bf9\u7ec6\u80de\u8fdb\u884c\u7b5b\u9009\u3002 3\u3001\u6839\u636e\u4e0a\u76ae\u7ec6\u80de\u7c98\u9644\u5206\u5b50\uff08EpCAM\uff09\u7684\u6570\u91cf\u6765\u7b5b\u9009\u764c\u7ec6\u80de Kelley\u7b49\u9996\u5148\u5c1d\u8bd5\u4e86\u8fd9\u79cd\u65b9\u5f0f\uff0c\u5e76\u4e14\u901a\u8fc7\u76f8\u5e94\u7684\u5b9e\u9a8c\u6765\u9a8c\u8bc1\u662f\u5426\u5176\u5177\u6709\u7b5b\u9009\u4e0d\u540c\u764c\u7ec6\u80de\u7684\u80fd\u529b\u3002\u5b9e\u9a8c\u4e2d\u4f7f\u7528MCF-7\u3001SKBR3\u3001PC-3\u3001MDA-MB-231\u56db\u79cd\u7ec6\u80de\u4e3a\u5bf9\u8c61\u3002\u4ed4\u7ec6\u8c03\u67e5\u8fd9\u4e9b\u7ec6\u80de\u7684\u4e0a\u76ae\u7ec6\u80de\u7c98\u9644\u5206\u5b50\u6570\u91cf\uff0c\u5e76\u4ee5\u6b64\u4e3a\u6807\u5fd7\u6765\u8fdb\u884c\u7ec6\u80de\u7b5b\u9009\uff08\u56fe4\uff09 \u56fe4\u6839\u636eEpCAM\u7684\u68c0\u51fa\u91cf\u4e0d\u540c\u5bf9\u4e8e\u764c\u7ec6\u80de\u8fdb\u884c\u7b5b\u9009\u3002\u56fe\u4e2d\u6240\u793a\u4e3a\u901a\u8fc7\u6d41\u5f0f\u7ec6\u80de\u4eea\u7b5b\u9009\u7684\u6570\u636e\u3002 \u8ba9\u7ec6\u80de\u5206\u522b\u6d41\u7ecfMagRC\uff0c\u56db\u79cd\u7ec6\u80de\u5c06\u4f1a\u5206\u5e03\u5728\u8bbe\u5907\u4e2d\u7684\u4e0d\u540c\u533a\u57df\u3002\u56e0\u4e3aMCF-7\u5728\u79bb\u6d41\u8def\u5165\u53e3\u6700\u8fd1\u7684\u4f4d\u7f6e\uff08Zone number\u6bd4\u8f83\u5c0f\uff0c\u4e14\u954d\u78c1\u4f53\u6bd4\u8f83\u5c0f\u7684\u5730\u65b9\uff09\u88ab\u6355\u6349\u5230\uff0c\u6240\u4ee5\u4e5f\u5c31\u8868\u660eEpCAM\u91cf\u8f83\u5927\u3002\u53e6\u4e00\u65b9\u9762\uff0c\u6211\u4eec\u5728\u51fa\u53e3\u9644\u8fd1\u6355\u6349\u5230\u4e86PC-3\u548cMDA-MB-231\uff0c\u8fd9\u8bf4\u660e\u5b83\u4eec\u7684EpCAM\u91cf\u5f88\u5c11\u3002\u5b9e\u9645\u4e0a\uff0c\u5982\u679c\u901a\u8fc7\u6d41\u5f0f\u7ec6\u80de\u6cd5\u7b5b\u9009\u8fd9\u56db\u79cd\u7ec6\u80de\u7684\u8bdd\uff0c\u4e0d\u96be\u7406\u89e3\u5176\u68c0\u51faEpCAM\u7684\u91cf\u662f\u548cMagRC\u7684\u68c0\u6d4b\u7ed3\u679c\u4e00\u81f4\u7684\u3002\u7279\u522b\u9700\u8981\u6ce8\u610f\u7684\u662f\uff0c\u548c\u6d41\u5f0f\u7ec6\u80de\u6cd5\u76f8\u6bd4\uff0c\u4f7f\u7528MagRC\u6cd5\uff0c\u89e3\u6790\u80fd\u529b\u4f1a\u66f4\u52a0\u51fa\u8272\u3002 4. \u8840\u6db2\u6837\u54c1\u4e2d\u764c\u7ec6\u80de\u7684\u6355\u6349 \u7531\u4e0a\u8ff0\u53ef\u77e5\uff0c\u5b9e\u9a8c\u6240\u7528\u7684\u6837\u672c\u53ea\u542b\u6709\u5355\u4e00\u79cd\u7c7b\u7684\u7ec6\u80de\uff0c\u5982\u679c\u8981\u8fd0\u7528\u4e8e\u5b9e\u9645\u4e2d\uff0c\u5219\u5c31\u5fc5\u987b\u8981\u80fd\u5728\u6df7\u6742\u7740\u7ea2\u7ec6\u80de\u548c\u767d\u7ec6\u80de\u7684\u8840\u6db2\u4e2d\u5206\u79bb\u51fa\u764c\u7ec6\u80de\u3002\u56e0\u6b64\uff0cKelly\u7b49\u4eba\u4f7f\u7528\u4eba\u4f53\u5168\u8840\uff0c\u4ee5EpCAM\u4e3a\u6807\u5fd7\u7269\u5bf9\u4e8e\u7ec6\u80de\u8fdb\u884c\u7b5b\u9009\u3002 \u56fe5 PBS\uff08\u4e0a\u65b9\u4e24\u56fe\uff09\u4ee5\u53ca\u5168\u8840\uff08\u4e0b\u65b9\u4e24\u56fe\uff09\u4e2d\u7684\u764c\u7ec6\u80de\u5206\u79bb\u3002\u5de6\u4fa7\u56fe\u4e3aMagRC\u4e2d\u6570\u636e\uff0c\u53f3\u4fa7\u56fe\u4e3a\u6d41\u5f0f\u7ec6\u80de\u4eea\u4e2d\u6570\u636e\u3002 \u56fe5\u5c55\u793a\u4e86\u57281ml\u5168\u8840\uff08\u6216\u8005PBS\uff09\u4e2d\u6df7\u5165100\u4e2a\u621610000\u4e2a\u764c\u7ec6\u80de\uff0c\u4f7f\u7528MagRC\u548c\u6d41\u5f0f\u7ec6\u80de\u4eea\u8fdb\u884c\u7ec6\u80de\u5206\u79bb\u65f6\u7684\u60c5\u51b5\u3002\u5728\u4f7f\u7528\u6d41\u5f0f\u7ec6\u80de\u4eea\u7684PBS\u6837\u672c\u4e2d\uff0c\u764c\u7ec6\u80de\u6570\u91cf\u5c11\u7684\u65f6\u5019\uff08100\u4e2a\uff0c\u5982\u53f3\u4e0a\u56fe\u6240\u793a\uff09\uff0c\u65e0\u6cd5\u5f97\u5230\u76f8\u5e94\u7684\u4fe1\u53f7\u3002\u5728\u5168\u8840\u6837\u672c\u4e2d\u4e5f\u4ec5\u80fd\u5f97\u5230\u672c\u5e95\u4fe1\u53f7\uff0c\u65e0\u6cd5\u68c0\u6d4b\u51fa\u764c\u7ec6\u80de\u3002 \u53e6\u4e00\u65b9\u9762\uff0c\u5728\u4f7f\u7528MagRC\u65f6\uff0c\u5373\u4f7f\u662f1ml\u4e2d\u53ea\u6df7\u5165100\u4e2a\u764c\u7ec6\u80de\u7684\u6837\u54c1\uff0c\u4e5f\u53ef\u4ee5\u505a\u5230\u6709\u6548\u5730\u5206\u79bb\u3002\u5728\u56fe5\u7684\u5de6\u4fa7\u4e0a\u4e0b\u4e24\u5e45\u56fe\u4e2d\uff0cPBS\u548c\u5168\u8840\u4e2d\u5747\u68c0\u51fa\u7ec6\u80de\uff0c\u7ecf\u786e\u8ba4\uff0c\u68c0\u51fa\u7684\u7ec6\u80de\u4e2d\u5e76\u6ca1\u6709\u9664\u764c\u7ec6\u80de\u4ee5\u5916\u7684\u7ec6\u80de\u3002 5\u3001\u764c\u75c7\u60a3\u8005\u7684\u8840\u6db2\u5206\u6790 \u540c\u65f6\uff0cKelley\u7b49\u4e5f\u4f7f\u7528MagRC\u7684\u65b9\u6cd5\u5bf9\u4e8e\u764c\u75c7\u60a3\u8005\u7684\u8840\u6db2\u6837\u672c\u8fdb\u884c\u5206\u6790\u3002\u4ece\u8f6c\u79fb\u6027\u53bb\u52bf\u62b5\u6297\u6027\u524d\u5217\u817a\u764c\uff08mCRPC\uff09\u4ee5\u53ca\u5c40\u9650\u6027\u524d\u5217\u817a\u764c\uff08localized prostate cancer\uff09\u60a3\u8005\u4e2d\u91c7\u96c6\u8840\u6db2\u6837\u672c\uff0c\u4f7f\u7528\u6307\u793a\u764c\u7ec6\u80de\u6076\u5316\u7a0b\u5ea6Gleason\u5206\u7ea7\uff08P1\u5230P14\uff09\u65b9\u6cd5\uff0c\u7ed8\u5236\u4e0b\u56fe\uff08\u56fe6\uff09 \u56fe6 \u5bf9\u4e0d\u540c\u79cd\u7c7b\u7684\u764c\u7ec6\u80deCTC\u5206\u5e03\u8fdb\u884c\u6bd4\u8f83\uff08\u6765\u81ea\u4e8e\u8bba\u6587\uff09\uff0c\u524d\u5217\u817a\u764c\uff08\u53f3\u56fe\uff09\u4e2d\uff0cGleason\u5206\u7ea7\u4e3aP1-3: 6\u3001P4-9: 7\u3001P10-14: 8-9\u3002 […]","protected":false},"author":8,"featured_media":17003,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","enabled":false},"version":2}},"categories":[1271],"tags":[2136,2134,2133,2135],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"https:\/\/assets.www.gsbet888.com\/uploads\/2017\/12\/1.png","jetpack_shortlink":"https:\/\/wp.me\/p96op1-4qe","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/posts\/17002"}],"collection":[{"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/comments?post=17002"}],"version-history":[{"count":7,"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/posts\/17002\/revisions"}],"predecessor-version":[{"id":27234,"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/posts\/17002\/revisions\/27234"}],"wp:featuredmedia":[{"embeddable":true,"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/media\/17003"}],"wp:attachment":[{"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/media?parent=17002"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/categories?post=17002"},{"taxonomy":"post_tag","embeddable":true,"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/tags?post=17002"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}