{"id":17632,"date":"2018-01-12T06:02:26","date_gmt":"2018-01-11T22:02:26","guid":{"rendered":"\/\/www.gsbet888.com\/?p=17632"},"modified":"2019-08-02T18:52:04","modified_gmt":"2019-08-02T10:52:04","slug":"nat-chem-%e4%bd%bf%e7%94%a8%e5%85%89%e5%82%ac%e5%8c%96%e5%89%82%e7%9a%84c-%e6%9c%ab%e7%ab%af%e9%80%89%e6%8b%a9%e6%80%a7%e8%84%b1%e7%be%a7%e5%9e%8bbioconjugation","status":"publish","type":"post","link":"\/\/www.gsbet888.com\/%e5%8c%96%e5%ad%a6%e6%9d%82%e8%ae%b0\/2018\/01\/nat-chem-%e4%bd%bf%e7%94%a8%e5%85%89%e5%82%ac%e5%8c%96%e5%89%82%e7%9a%84c-%e6%9c%ab%e7%ab%af%e9%80%89%e6%8b%a9%e6%80%a7%e8%84%b1%e7%be%a7%e5%9e%8bbioconjugation.html","title":{"rendered":"Nat. Chem. \u5149\u50ac\u5316\u4f53\u7cfb\u7684C-\u672b\u7aef\u9009\u62e9\u6027\u8131\u7fa7\u578bbioconjugation"},"content":{"rendered":"<p><strong>\u5149\u50ac\u5316\u9886\u57df\u7684&#8221;\u5927\u54e5\u5927&#8221;<a href=\"\/\/www.gsbet888.com\/chemists\/2014\/11\/post-3283.html\">MacMillan\u6559\u6388<\/a>\u8fd9\u56de\u662f\u8981\u8fdb\u5165\u5316\u5b66\u751f\u7269\u5b66\u9886\u57df\u7684\u8282\u594f\uff1f<\/strong><\/p>\n<blockquote>\n<div class=\"page\" title=\"Page 1\">\n<div class=\"layoutArea\">\n<div class=\"column\">\n<p>Decarboxylative alkylation for site-selective\u00a0bioconjugation of native proteins via oxidation\u00a0potentials<\/p>\n<p><span class=\"Apple-style-span\">Bloom, S.; Liu, C.; K\u00f6lmel, D.; Qiao, J.; Zhang, Y.; Poss, M.; Ewing, W.; MacMillan, D.<\/span><\/p>\n<p><span class=\"Apple-style-span\"><i>Nat. Chem. <\/i>DOI:\u00a0<\/span><a href=\"http:\/\/dx.doi.org\/10.1038\/NCHEM.2888\">10.1038\/NCHEM.2888<\/a><\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/blockquote>\n<p>\u8fd9\u4e00\u6b21\u4ed6\u4eec\u628a\u5149\u50ac\u5316\u5e94\u7528\u5728\u4e86\u5c0f\u5206\u5b50\u53cd\u5e94\u65b9\u6cd5\u5b66\u5f00\u53d1\u7684\u4eba\u6765\u8bf4\u6bd4\u8f83\u5bb9\u6613\u4e0a\u624b\u7684bioconjugation\u4e0a\uff08\u7eaf\u5c5e\u4e2a\u4eba\u81c6\u65ad\uff09\u3002<\/p>\n<p>bioconjugation\u662f\u4ec0\u4e48\uff1f\u653e\u5fc3\uff0c<a href=\"\/\/www.gsbet888.com\/reactions\/2017\/12\/%e7%94%9f%e7%89%a9%e5%85%b1%e8%bd%ad%ef%bc%88%e5%81%b6%e8%81%94%ef%bc%89-bioconjugation.html\"><strong>\u8bf7\u6233\u6211<\/strong><\/a>\uff0cchem-station\u65e9\u5c31\u6709\u8be5\u8bcd\u6761\u7684\u8be6\u7ec6\u6587\u7ae0\u4ecb\u7ecd\u3002<span id=\"more-54603\"><\/span><\/p>\n<h4>Bioconjugation\u7684\u5fc5\u8981\u6761\u4ef6\u6709<\/h4>\n<p>bioconjugation\u662f\u4ee5\u591a\u80bd\/\u86cb\u767d\u8d28\u4e3a\u5e95\u7269\uff0c<\/p>\n<ol>\n<li>\u53cd\u5e94\u5fc5\u987b\u5728\u542b\u6c34\u4f53\u7cfb\u4e2d\u8fdb\u884c\u3002<\/li>\n<li>\u4e00\u822c\u6765\u8bf4\u86cb\u767d\u8d28\u6eb6\u6db2\u65e0\u6cd5\u914d\u7f6e\u6210\u9ad8\u6d53\u5ea6\u7684\uff08\u6eb6\u89e3\u6027\u4f4e\uff09\uff0c\u6240\u4ee5\u53cd\u5e94\u5fc5\u987b\u80fd\u5728\u4f4e\u6d53\u5ea6\u6761\u4ef6\u4e0b\u4e5f\u80fd\u8fdb\u884c\u3002<\/li>\n<li>\u6761\u4ef6\u5fc5\u987b\u6e29\u548c \uff08\u907f\u514d\u7834\u574f\u86cb\u767d\u8d28\u7684\u4e09\u7ea7\u7ed3\u6784\uff09<\/li>\n<li>\u5fc5\u987b\u5177\u6709\u9ad8\u9009\u62e9\u6027\u3002(\u591a\u79cd\u5b98\u80fd\u56e2\u5171\u5b58)<\/li>\n<\/ol>\n<p>\u8fd9\u662f\u76f8\u5f53\u56f0\u96be\u7684\u6761\u4ef6\uff0c\u800cMacmillan\u7684\u65b9\u6cd5\u663e\u7136\u6ee1\u8db3\u4e86\u8fd9\u4e9b\u5fc5\u8981\u6761\u4ef6\u3002<\/p>\n<p>&nbsp;<\/p>\n<h4>\u6c34\u4e2d\u7684\u53cd\u5e94\u6027\u4e0e\u5b98\u80fd\u56e2\u9009\u62e9\u6027<\/h4>\n<p>\u5728\u8fd9\u6b21\u7684\u8bba\u6587\u4e2d\uff0c\u4ed6\u4eec\u4f7f\u7528\u5149\u50ac\u5316\u5242\u4ea7\u751f\u81ea\u7531\u57fa\u8131\u7fa7\u5e76\u8fdb\u800c\u8fdb\u884c\u5b98\u80fd\u56e2\u5316\u53cd\u5e94\u3002\u8fc4\u4eca\u4e3a\u6b62\uff0c\u5c31\u53cd\u5e94\u6027\u800c\u8a00\uff0c\u5df2\u7ecf\u6709\u62a5\u9053\u8868\u660e\u5177\u6709\u9ad8\u53cd\u5e94\u6027\u7684\u81ea\u7531\u57fa\u53cd\u5e94\u5728\u6c34\u4e2d\u7684\u5bf9\u86cb\u767d\u8d28\u4fee\u9970\u4e5f\u662f\u53ef\u884c\u7684<sup>[<\/sup><sup>1]<\/sup>\u3001\u56e0\u6b64\uff0c\u672c\u53cd\u5e94\u4f53\u7cfb\u7528\u4e8ebioconjugation\u4e5f\u662f\u6bd4\u8f83\u201c\u987a\u5176\u81ea\u7136\u201d\u3002<\/p>\n<div id=\"attachment_54805\" class=\"wp-caption aligncenter\">\n<p><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-54805 size-full\" src=\"https:\/\/i2.wp.com\/assets.chem-station.com\/uploads\/2017\/12\/scheme.jpg?resize=4625%2C816&amp;ssl=1\" alt=\"\" width=\"648\" height=\"114\" \/><\/p>\n<p class=\"wp-caption-text\">\u81ea\u7531\u57fa\u53cd\u5e94\u5e94\u7528\u4e8eBioconjugation<\/p>\n<\/div>\n<p>&nbsp;<\/p>\n<p>\u63a5\u4e0b\u6765\u662f\u9009\u62e9\u6027\u7684\u95ee\u9898\u3002\u8fd9\u6b21\u62a5\u9053\u7684\u8bba\u6587\u662fC\u672b\u7aef\u9009\u62e9\u6027\u7684\u8131\u7fa7\u578b\u5b98\u80fd\u56e2\u5316\u53cd\u5e94\u3002\u5728\u86cb\u767d\u8d28\u4e2d\u9664\u4e86\u78b3\u672b\u7aef\u4ee5\u5916\uff0c\u5929\u51ac\u6c28\u9178\u548c\u8c37\u6c28\u9178\u7684\u4fa7\u94fe\u4e0a\u4e5f\u5e26\u6709\u7fa7\u9178\u57fa\u56e2\uff0c\u7136\u800c\uff0c\u5728\u82ef\u53cd\u5e94\u4e2d\uff0c\u76f8\u5bf9\u4e0e\u5929\u51ac\u6c28\u9178\u4e0e\u8c37\u6c28\u9178\u7684\u4fa7\u94fe\u7fa7\u9178\u57fa\u56e2\u6765\u8bf4\uff0c\u53cd\u5e94\u9009\u62e9\u6027\u7684\u53d1\u751f\u5728\u5fae\u91cf\u7684\u7684C\u672b\u7aef\u7684\u7fa7\u9178\uff08<em>C-terminalcarboxylate<\/em>\uff09\u4e0a\u3002\u78b3\u672b\u7aef\u7684\u7fa7\u9178\u8131\u7fa7\u540e\u5f62\u6210\u7684\u81ea\u7531\u57fa\u7531\u4e8e\u90bb\u4f4d\u6709\u6742\u539f\u5b50\uff08\u6c2e\u539f\u5b50\uff09\u7684\u5b58\u5728\uff0c\u56e0\u6b64\u5b83\u6bd4\u7531\u5929\u51ac\u6c28\u9178\u6216\u8c37\u6c28\u9178\u4fa7\u94fe\u4ea7\u751f\u7684\u81ea\u7531\u57fa\u66f4\u7a33\u5b9a\u3002\u8fd9\u5c31\u662f\u4e3a\u4ec0\u4e48C\u672b\u7aef\u9009\u62e9\u6027\u7684\u53d1\u751f\u8131\u7fa7\u53cd\u5e94\u7684\u7f18\u7531\u3002<\/p>\n<div id=\"attachment_54802\" class=\"wp-caption aligncenter\">\n<p><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-54802 size-full\" src=\"https:\/\/i1.wp.com\/assets.chem-station.com\/uploads\/2017\/12\/b47ec129aee217ee482a91de4e8db199.jpg?resize=5816%2C1958&amp;ssl=1\" alt=\"\" width=\"648\" height=\"218\" \/><\/p>\n<p class=\"wp-caption-text\">\u53cd\u5e94\u7684\u9009\u62e9\u6027\uff1a\u4fa7\u94fe\u7684\u7fa7\u9178 vs C\u672b\u7aef\u7684\u7fa7\u9178<\/p>\n<\/div>\n<h4>\u5149\u50ac\u5316\u5242\u7684\u63a2\u8ba8<\/h4>\n<p>\u5728\u5927\u81f4\u4e86\u89e3\u4e3a\u4f55\u5728\u78b3\u672b\u7aef\u7684\u7fa7\u9178\u4e0a\u53d1\u751f\u9009\u62e9\u6027\u53cd\u5e94\u540e\u3002\u4e5f\u5c31\u662f\u8bf4MacMillan\u4ed6\u4eec\u628a\u4ee5\u524d\u7528\u5728\u5c0f\u5206\u5b50\u4e2d\u7684\u65b9\u6cd5\u5b66\u7528\u5230\u86cb\u767d\u8d28\u4e0a\u5c31\u641e\u5b9a\u4e86\uff0c\u7136\u800c\uff0c\u5b9e\u9645\u4e0a\u5e76\u975e\u8fd9\u4e48\u7b80\u5355\u3002<\/p>\n<p>\u4f5c\u8005\u53d1\u73b0\uff0c\u672c\u53cd\u5e94\u5728\u5e38\u7528\u7684\u53cd\u5e94\u4f53\u7cfb-\u6c34\u6eb6\u6027\u954d\u50ac\u5316\u5242\u4e0e\u6709\u673a\u5149\u654f\u8bd5\u5242\u7684\u6761\u4ef6\u4e0b\uff0c\u53cd\u5e94\u5e76\u4e0d\u80fd\u8fdb\u884c\u3002\u56e0\u6b64\uff0c\u4ed6\u4eec\u7b5b\u9009\u540e\u9009\u62e9flavin\uff08\u9ec4\u7d20\u884d\u751f\u7269\uff09\u4f5c\u4e3aSET\u50ac\u5316\u5242\uff0c\u56e0\u4e3a\u6709\u62a5\u9053\u8868\u660eflavin\u53ef\u4ee5\u50ac\u5316\u03b1-\u53d6\u4ee3\u7684\u4e59\u9178\u916f\u7684\u8131\u7fa7\u53cd\u5e94\uff0c\u4f46\u662f\u5b83\u662f\u4e8c\u7535\u5b50\u8f6c\u79fb\u53cd\u5e94\u3002\u770b\u4f3cflavin\u5e76\u4e0d\u9002\u5408\u7528\u5728\u4f46\u7535\u5b50\u79fb\u52a8\u53cd\u5e94\uff08SET\uff09\u4e0a\uff0c\u7136\u800c\uff0c\u771f\u6b63\u8bd5\u4e86\u540e\u53d1\u73b0\u6548\u679c\u975e\u5e38\u597d\u3002<\/p>\n<div id=\"attachment_54801\" class=\"wp-caption aligncenter\">\n<p><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-54801 size-full\" src=\"https:\/\/i0.wp.com\/assets.chem-station.com\/uploads\/2017\/12\/8c8bc0dd9c7fc98ef8f56b5535a62e55.jpg?resize=5566%2C2258&amp;ssl=1\" alt=\"\" width=\"648\" height=\"263\" \/><\/p>\n<p class=\"wp-caption-text\">\u53cd\u5e94\u673a\u7406: <b>flavin<\/b>\u4f5c\u4e3aSET\u50ac\u5316\u5242\u4f5c\u7528<\/p>\n<\/div>\n<h4>Limitation\u662f\uff1f<\/h4>\n<p>\u4e00\u822c\u5bf9\u4e8e\u81ea\u7531\u57fa\u53cd\u5e94\u6765\u8bf4\u6bd4\u8f83\u5728\u610f\u7684\u7b2c\u4e00\u611f\u89c9\u5c31\u662f\u5b98\u80fd\u56e2\u7684\u517c\u5bb9\u6027\u3002\u5728\u62e5\u6709\u4ee5\u4e0b\u6c28\u57fa\u9178\u7684\u86cb\u767d\u8d28\u4e2d\uff0c<\/p>\n<p>Lys\u4e0eHis\u2192\u9178\u6027\u6761\u4ef6\u4e0b\uff0c\u53cd\u5e94\u80fd\u591f\u987a\u5229\u8fdb\u884c\u3002<\/p>\n<p>Tyr\u2192\u4f4e\u6d3b\u6027\u7684flavin\u50ac\u5316\u5242\u6761\u4ef6\u4e0b\u53cd\u5e94\u53ef\u4ee5\u987a\u5229\u8fdb\u884c\uff0c\u4f46\u662f\u4ea7\u7387\u6bd4\u8f83\u4f4e\u3002Tyr\u5177\u6709\u6f5c\u5728\u7684\u8fd8\u539f\u6027\uff0c\u6240\u4ee5\u6bd4\u8f83\u5bb9\u6613\u88ab\u6c27\u5316\u3002<\/p>\n<p>Cys\u3001Met\u3001Trp\u2192\u5177\u6709\u8fd9\u4e9b\u6c28\u57fa\u9178\u7684\u591a\u80bd\/\u86cb\u767d\u8d28\u7684\u5e95\u7269\u6ca1\u6709\u5728\u8bba\u6587\u4e2d\u51fa\u73b0\u3002\u5bf9\u4e8eCys\uff0c\u7531\u4e8e\u8fd9\u6b21\u53cd\u5e94\u4e2d\u7528\u5230\u4e86\u8fc8\u514b\u5c14\u53d7\u4f53\uff0c\u56e0\u6b64\u4f30\u8ba1\u8be5\u6761\u4ef6\u5f88\u96be\u9002\u7528\u4e8eCys\u3002\u5bf9\u4e8eMet\u4e0eTrp\uff0c\u786b\u919a\u548c\u5432\u54da\u7684\u6c27\u5316\u53cd\u5e94\u5e94\u8be5\u4f1a\u4f18\u5148\u8fdb\u884c\u3002<\/p>\n<p>\u4e0a\u8ff0\u4ee5\u5916\u7684\u6c28\u57fa\u9178\u90fd\u9002\u7528\u4e8e\u672c\u6761\u4ef6\u3002<\/p>\n<h4>\u80f0\u5c9b\u7d20\u7684\u5b98\u80fd\u56e2\u5316<\/h4>\n<p>\u4f5c\u4e3a\u672c\u624b\u6cd5\u7684\u5e94\u7528\uff0c\u4f5c\u8005\u5c1d\u8bd5\u7740\u5e94\u7528\u4e8e\u80f0\u5c9b\u7d20\u7684\u5b98\u80fd\u5316\u3002\u4e5f\u5c31\u662f\u8bf4\uff0c\u8be5\u86cb\u767d\u8d28\u6709\u4e24\u5904\u5177\u6709\u78b3\u672b\u7aef\u7fa7\u9178\u7ed3\u6784\uff0c\u53e6\u5916\uff0c\u8fd9\u4e24\u6761\u80bd\u94fe\u542b\u6709\u56db\u4e2a\u8c37\u6c28\u9178\u6b8b\u57fa\u3002\u6b64\u5916\uff0c\u5728\u8fd9\u4e2a\u53cd\u5e94\u4f53\u7cfb\u4e2d\u6709\u672c\u53cd\u5e94\u4f53\u7cfb\u6bd4\u8f83\u96be\u641e\u5b9a\u7684\u56db\u4e2a\u916a\u6c28\u9178\u6b8b\u57fa\uff0c\u4e5f\u5c31\u662f\u8bf4\u80f0\u5c9b\u7d20\u5176\u5b9e\u662f\u4e00\u4e2a\u5177\u6709\u6311\u6218\u6027\u7684\u76ee\u6807\u5e95\u7269\u3002<\/p>\n<p>\u4f46\u662f\uff0c\u5b9e\u9645\u5728\u5c1d\u8bd5\u540e\u53d1\u73b0\uff0cA-chain\u4f18\u5148\u53d1\u751f\u4e86C\u672b\u7aef\u7fa7\u9178\u7684\u5b98\u80fd\u56e2\u5316\u53cd\u5e94\u3002\u5f53\u7136\uff0c\u4e8c\u786b\u952e\uff0c\u8c37\u6c28\u9178\u7684\u7fa7\u9178\u548c\u5176\u4ed6\u6742\u539f\u5b50\u90fd\u88ab\u4fdd\u7559\u4e0b\u6765\uff0c\u5e76\u6ca1\u6709\u53d7\u5230\u5f71\u54cd\u3002<\/p>\n<div id=\"attachment_54803\" class=\"wp-caption aligncenter\">\n<p><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-54803 size-full\" src=\"https:\/\/i1.wp.com\/assets.chem-station.com\/uploads\/2017\/12\/cd237eb8e49e8495b95c8f08e4f650fe.jpg?resize=1648%2C1290&amp;ssl=1\" alt=\"\" width=\"648\" height=\"507\" \/><\/p>\n<p class=\"wp-caption-text\">\u5e94\u7528\u4e8e\u80f0\u5c9b\u7d20<\/p>\n<\/div>\n<p>&nbsp;<\/p>\n<p>\u5bf9\u4e8eA-chain\u4f18\u5148\u4e8eB-chain\u53d1\u751f\u53cd\u5e94\uff0c\u4f5c\u8005\u7ed9\u51fa\u4e86\u4ee5\u4e0b\u4e24\u70b9\u53ef\u80fd\u7684\u7406\u7531\u3002<\/p>\n<p>\u5176\u4e2d\u4e00\u70b9\u662f\u5355\u7eaf\u7684A-chain\u7684\u78b3\u672b\u7aef\u6bd4B-chain\u7684\u66f4\u5bb9\u6613\u88ab\u6c27\u5316\u3002<\/p>\n<p>\u53e6\u4e00\u70b9\u4ed6\u4eec\u8ba4\u4e3a\u6709\u53ef\u80fd\u662f\uff0cA-chain\u7684\u78b3\u672b\u7aef\u8fd1\u65c1\u7684\u758f\u6c34\u8868\u9762\u66f4\u5bb9\u6613&#8221;\u805a\u96c6&#8221;\u5149\u50ac\u5316\u5242\uff0c\u901a\u8fc7\u8be5\u8fd1\u65c1\u6548\u679c\uff0cA-chain\u7684C\u672b\u7aef\u4f18\u5148\u53d1\u751f\u53cd\u5e94\u3002\u867d\u7136\u8fd9\u4e24\u4e2a\u53ef\u80fd\u6027\u5047\u8bf4\u5e76\u6ca1\u6709\u76f4\u63a5\u7684\u8bc1\u636e\uff0c\u4f46\u662fA-chain\u4e0eB-chain\u7684\u9009\u62e9\u6027\u5dee\u5f02\u8fd9\u4e00\u53d1\u73b0\u8fd8\u662f\u975e\u5e38\u6709\u8da3\u7684\u3002<\/p>\n<p>&nbsp;<\/p>\n<h4>\u7ed3\u5c3e<\/h4>\n<p>\u5b8c\u7f8e\u7684bioconjugation\u7684\u65b9\u6cd5\u5b66\u8fd8\u5e76\u6ca1\u6709\u88ab\u5f00\u53d1\u51fa\u6765\uff0c\u9884\u8ba1\u8fd8\u6709\u5f88\u957f\u7684\u8def\u8981\u8d70\uff0c\u4f46\u4e0d\u53ef\u5426\u8ba4\uff0c\u8fd9\u4f1a\u662f\u4e00\u4e2a\u975e\u5e38\u70ed\u70b9\u7684\u8bfe\u9898\u3002<\/p>\n<p>\u8fd9\u6b21\uff0cMacmillan\u7ec4\u4ec5\u4ec5\u662f\u5c1d\u8bd5\u5e94\u7528\u4e8ebioconjugation\uff0c\u800c\u672a\u6765\u6216\u8005\u662f\u5426\u76ee\u524d\u5df2\u7ecf\u5728\u5c1d\u8bd5\u5e94\u7528\u4e8ebioorthogonal\u53cd\u5e94\uff0c\u751a\u81f3\u662f\u6d3b\u7ec6\u80de\u4e2d\u5929\u7136\u86cb\u767d\u8d28\u7684\u6807\u8bb0\u7b49\u7b49\uff1f\u8ba9\u6211\u4eec\u62ed\u76ee\u4ee5\u5f85\u3002<\/p>\n<h4>\u53c2\u8003\u6587\u732e<\/h4>\n[1]<span class=\"Apple-style-span\">Sato, S.; Nakamura, H. <\/span><em>Angew. Chem., Int. Ed.<\/em> <span class=\"Apple-style-span\">2013, <i>52<\/i>, 8681<\/span>. DOI:<a href=\"http:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/anie.201303831\/abstract\">10.1002\/anie.201303831<\/a><\/p>\n<p>&nbsp;<\/p>\n<h4>\u5173\u8054\u4e66\u7c4d\uff08\u65e5\u4e9a\u4ef7\u683c\uff09<\/h4>\n<div class=\"amazonjs_item amazonjs_book\">\n<div class=\"amazonjs_image\"><a class=\"amazonjs_link\" title=\"Bioconjugation: Protein Coupling Techniques for the Biomedical Sciences\" href=\"https:\/\/www.amazon.co.jp\/Bioconjugation-Coupling-Techniques-Biomedical-Sciences\/dp\/1561591610?SubscriptionId=AKIAI6Q3RDQBU2J6P3JQ&amp;tag=chemstation-22&amp;linkCode=xm2&amp;camp=2025&amp;creative=165953&amp;creativeASIN=1561591610\" target=\"_blank\" rel=\"noopener\" data-role=\"amazonjs_product\" data-asin=\"1561591610\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/images-fe.ssl-images-amazon.com\/images\/I\/411bpmzqwcL._SL160_.jpg\" alt=\"Bioconjugation: Protein Coupling Techniques for the Biomedical Sciences\" width=\"107\" height=\"160\" \/><\/a><\/div>\n<div class=\"amazonjs_info\">\n<h4><a class=\"amazonjs_link\" title=\"Bioconjugation: Protein Coupling Techniques for the Biomedical Sciences\" href=\"https:\/\/www.amazon.co.jp\/Bioconjugation-Coupling-Techniques-Biomedical-Sciences\/dp\/1561591610?SubscriptionId=AKIAI6Q3RDQBU2J6P3JQ&amp;tag=chemstation-22&amp;linkCode=xm2&amp;camp=2025&amp;creative=165953&amp;creativeASIN=1561591610\" target=\"_blank\" rel=\"noopener\" data-role=\"amazonjs_product\" data-asin=\"1561591610\">Bioconjugation: Protein Coupling Techniques for the Biomedical Sciences<\/a><\/h4>\n<ul>\n<li><b>\u8457\u8005<\/b>Mohammed Aslam,Alisdair Dent<\/li>\n<li class=\"amazonjs_price\" title=\"\u4fa1\u683c\u304a\u3088\u3073\u767a\u9001\u53ef\u80fd\u6642\u671f\u306f\u8868\u793a\u3055\u308c\u305f\u65e5\u4ed8\/\u6642\u523b\u306e\u6642\u70b9\u306e\u3082\u306e\u3067\u3042\u308a\u3001\u5909\u66f4\u3055\u308c\u308b\u5834\u5408\u304c\u3042\u308a\u307e\u3059\u3002\u5546\u54c1\u306e\u8ca9\u58f2\u306b\u304a\u3044\u3066\u306f\u3001\u8cfc\u5165\u306e\u6642\u70b9\u3067 [Amazon.co.jp \u307e\u305f\u306f Javari.jp] \u306b\u8868\u793a\u3055\u308c\u3066\u3044\u308b\u4fa1\u683c\u304a\u3088\u3073\u767a\u9001\u53ef\u80fd\u6642\u671f\u306e\u60c5\u5831\u304c\u9069\u7528\u3055\u308c\u307e\u3059\u3002\"><b>\u53c2\u8003\u4fa1\u683c<\/b><span class=\"amazonjs_listprice\">\uffe5 20,236<\/span><br \/>\n<b>\u4fa1\u683c<\/b>\uffe5 9,133(2018\/01\/09 19:38\u6642\u70b9)<\/li>\n<li><b>\u51fa\u7248\u65e5<\/b>1998\/10\/01<\/li>\n<li><b>\u30cf\u30fc\u30c9\u30ab\u30d0\u30fc<\/b>833\u30da\u30fc\u30b8<\/li>\n<li><b>ISBN-10<\/b>1561591610<\/li>\n<li><b>ISBN-13<\/b>9781561591619<\/li>\n<li><b>\u51fa\u7248\u793e<\/b>Nature Pub Group<\/li>\n<\/ul>\n<\/div>\n<p><a class=\"amazonjs_review\" title=\"Bioconjugation: Protein Coupling Techniques for the Biomedical Sciences \u30ab\u30b9\u30bf\u30de\u30fc\u30ec\u30d3\u30e5\u30fc\" href=\"https:\/\/www.amazon.jp\/reviews\/iframe?akid=AKIAI6Q3RDQBU2J6P3JQ&amp;alinkCode=xm2&amp;asin=1561591610&amp;atag=chemstation-22&amp;exp=2018-01-10T10%3A38%3A30Z&amp;v=2&amp;sig=SgmeB%252BDkA5T9YYodx5bKJ17lOusf3VOq7FF0sFXB%252BmQ%253D&amp;TB_iframe=true&amp;height=500&amp;width=600\" target=\"_blank\" rel=\"noopener\">\u30ab\u30b9\u30bf\u30de\u30fc\u30ec\u30d3\u30e5\u30fc\u3092\u898b\u308b<\/a><\/p>\n<div class=\"amazonjs_footer\"><\/div>\n<\/div>\n<div class=\"amazonjs_item amazonjs_book\">\n<div class=\"amazonjs_image\"><a class=\"amazonjs_link\" title=\"Bioconjugation Protocols: Strategies and Methods (Methods in Molecular Biology)\" href=\"https:\/\/www.amazon.co.jp\/Bioconjugation-Protocols-Strategies-Methods-Molecular\/dp\/1617373540?SubscriptionId=AKIAI6Q3RDQBU2J6P3JQ&amp;tag=chemstation-22&amp;linkCode=xm2&amp;camp=2025&amp;creative=165953&amp;creativeASIN=1617373540\" target=\"_blank\" rel=\"noopener\" data-role=\"amazonjs_product\" data-asin=\"1617373540\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/images-fe.ssl-images-amazon.com\/images\/I\/51Bj7-QerkL._SL160_.jpg\" alt=\"Bioconjugation Protocols: Strategies and Methods (Methods in Molecular Biology)\" width=\"100\" height=\"160\" \/><\/a><\/div>\n<div class=\"amazonjs_info\">\n<h4><a class=\"amazonjs_link\" title=\"Bioconjugation Protocols: Strategies and Methods (Methods in Molecular Biology)\" href=\"https:\/\/www.amazon.co.jp\/Bioconjugation-Protocols-Strategies-Methods-Molecular\/dp\/1617373540?SubscriptionId=AKIAI6Q3RDQBU2J6P3JQ&amp;tag=chemstation-22&amp;linkCode=xm2&amp;camp=2025&amp;creative=165953&amp;creativeASIN=1617373540\" target=\"_blank\" rel=\"noopener\" data-role=\"amazonjs_product\" data-asin=\"1617373540\">Bioconjugation Protocols: Strategies and Methods (Methods in Molecular Biology)<\/a><\/h4>\n<ul>\n<li class=\"amazonjs_price\" title=\"\u4fa1\u683c\u304a\u3088\u3073\u767a\u9001\u53ef\u80fd\u6642\u671f\u306f\u8868\u793a\u3055\u308c\u305f\u65e5\u4ed8\/\u6642\u523b\u306e\u6642\u70b9\u306e\u3082\u306e\u3067\u3042\u308a\u3001\u5909\u66f4\u3055\u308c\u308b\u5834\u5408\u304c\u3042\u308a\u307e\u3059\u3002\u5546\u54c1\u306e\u8ca9\u58f2\u306b\u304a\u3044\u3066\u306f\u3001\u8cfc\u5165\u306e\u6642\u70b9\u3067 [Amazon.co.jp \u307e\u305f\u306f Javari.jp] \u306b\u8868\u793a\u3055\u308c\u3066\u3044\u308b\u4fa1\u683c\u304a\u3088\u3073\u767a\u9001\u53ef\u80fd\u6642\u671f\u306e\u60c5\u5831\u304c\u9069\u7528\u3055\u308c\u307e\u3059\u3002\"><b>\u4fa1\u683c<\/b>\uffe5 15,821(2018\/01\/09 19:38\u6642\u70b9)<\/li>\n<li><b>\u51fa\u7248\u65e5<\/b>2010\/11\/10<\/li>\n<li><b>\u30da\u30fc\u30d1\u30fc\u30d0\u30c3\u30af<\/b>344\u30da\u30fc\u30b8<\/li>\n<li><b>ISBN-10<\/b>1617373540<\/li>\n<li><b>ISBN-13<\/b>9781617373541<\/li>\n<li><b>\u51fa\u7248\u793e<\/b>Humana Press<\/li>\n<\/ul>\n<\/div>\n<p><a class=\"amazonjs_review\" title=\"Bioconjugation Protocols: Strategies and Methods (Methods in Molecular Biology) \u30ab\u30b9\u30bf\u30de\u30fc\u30ec\u30d3\u30e5\u30fc\" href=\"https:\/\/www.amazon.jp\/reviews\/iframe?akid=AKIAI6Q3RDQBU2J6P3JQ&amp;alinkCode=xm2&amp;asin=1617373540&amp;atag=chemstation-22&amp;exp=2018-01-10T10%3A38%3A30Z&amp;v=2&amp;sig=HP%252FRd2aUahTm3LF4dB9o0TmdZ1FK6ywZSzPKjySwkI0%253D&amp;TB_iframe=true&amp;height=500&amp;width=600\" target=\"_blank\" rel=\"noopener\">\u30ab\u30b9\u30bf\u30de\u30fc\u30ec\u30d3\u30e5\u30fc\u3092\u898b\u308b<\/a><\/p>\n<div class=\"amazonjs_footer\"><\/div>\n<\/div>\n<div class=\"amazonjs_item amazonjs_book\">\n<div class=\"amazonjs_image\"><a class=\"amazonjs_link\" title=\"Bioconjugation Protocols: Strategies and Methods (Methods in Molecular Biology)\" href=\"https:\/\/www.amazon.co.jp\/Bioconjugation-Protocols-Strategies-Methods-Molecular\/dp\/1617791504?SubscriptionId=AKIAI6Q3RDQBU2J6P3JQ&amp;tag=chemstation-22&amp;linkCode=xm2&amp;camp=2025&amp;creative=165953&amp;creativeASIN=1617791504\" target=\"_blank\" rel=\"noopener\" data-role=\"amazonjs_product\" data-asin=\"1617791504\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/images-fe.ssl-images-amazon.com\/images\/I\/416ODY0N-oL._SL160_.jpg\" alt=\"Bioconjugation Protocols: Strategies and Methods (Methods in Molecular Biology)\" width=\"116\" height=\"160\" \/><\/a><\/div>\n<div class=\"amazonjs_info\">\n<h4><a class=\"amazonjs_link\" title=\"Bioconjugation Protocols: Strategies and Methods (Methods in Molecular Biology)\" href=\"https:\/\/www.amazon.co.jp\/Bioconjugation-Protocols-Strategies-Methods-Molecular\/dp\/1617791504?SubscriptionId=AKIAI6Q3RDQBU2J6P3JQ&amp;tag=chemstation-22&amp;linkCode=xm2&amp;camp=2025&amp;creative=165953&amp;creativeASIN=1617791504\" target=\"_blank\" rel=\"noopener\" data-role=\"amazonjs_product\" data-asin=\"1617791504\">Bioconjugation Protocols: Strategies and Methods (Methods in Molecular Biology)<\/a><\/h4>\n<ul>\n<li class=\"amazonjs_price\" title=\"\u4fa1\u683c\u304a\u3088\u3073\u767a\u9001\u53ef\u80fd\u6642\u671f\u306f\u8868\u793a\u3055\u308c\u305f\u65e5\u4ed8\/\u6642\u523b\u306e\u6642\u70b9\u306e\u3082\u306e\u3067\u3042\u308a\u3001\u5909\u66f4\u3055\u308c\u308b\u5834\u5408\u304c\u3042\u308a\u307e\u3059\u3002\u5546\u54c1\u306e\u8ca9\u58f2\u306b\u304a\u3044\u3066\u306f\u3001\u8cfc\u5165\u306e\u6642\u70b9\u3067 [Amazon.co.jp \u307e\u305f\u306f Javari.jp] \u306b\u8868\u793a\u3055\u308c\u3066\u3044\u308b\u4fa1\u683c\u304a\u3088\u3073\u767a\u9001\u53ef\u80fd\u6642\u671f\u306e\u60c5\u5831\u304c\u9069\u7528\u3055\u308c\u307e\u3059\u3002\"><b>\u53c2\u8003\u4fa1\u683c<\/b><span class=\"amazonjs_listprice\">\uffe5 23,766<\/span><br \/>\n<b>\u4fa1\u683c<\/b>\uffe5 14,974(2018\/01\/09 21:33\u6642\u70b9)<\/li>\n<li><b>\u51fa\u7248\u65e5<\/b>2011\/06\/20<\/li>\n<li><b>\u5546\u54c1\u30e9\u30f3\u30ad\u30f3\u30b0<\/b>547,289\u4f4d<\/li>\n<li><b>\u30cf\u30fc\u30c9\u30ab\u30d0\u30fc<\/b>605\u30da\u30fc\u30b8<\/li>\n<li><b>ISBN-10<\/b>1617791504<\/li>\n<li><b>ISBN-13<\/b>9781617791505<\/li>\n<li><b>\u51fa\u7248\u793e<\/b>Humana Press<\/li>\n<\/ul>\n<\/div>\n<p><a class=\"amazonjs_review\" title=\"Bioconjugation Protocols: Strategies and Methods (Methods in Molecular Biology) \u30ab\u30b9\u30bf\u30de\u30fc\u30ec\u30d3\u30e5\u30fc\" href=\"https:\/\/www.amazon.jp\/reviews\/iframe?akid=AKIAI6Q3RDQBU2J6P3JQ&amp;alinkCode=xm2&amp;asin=1617791504&amp;atag=chemstation-22&amp;exp=2018-01-10T12%3A33%3A17Z&amp;v=2&amp;sig=Ph%252FkWkohNniejw3VrnK2CaPio5NfqsFT0C3B3%252BArk8M%253D&amp;TB_iframe=true&amp;height=500&amp;width=600\" target=\"_blank\" rel=\"noopener\">\u30ab\u30b9\u30bf\u30de\u30fc\u30ec\u30d3\u30e5\u30fc\u3092\u898b\u308b<\/a><\/p>\n<\/div>\n<p>&nbsp;<\/p>\n<p><strong>\u672c\u6587\u7248\u6743\u5c5e\u4e8e\u00a0<a href=\"\/\/www.gsbet888.com\/\">Chem-Station\u5316\u5b66\u7a7a\u95f4<\/a>\uff0c \u6b22\u8fce\u70b9\u51fb\u6309\u94ae\u5206\u4eab\uff0c\u672a\u7ecf\u8bb8\u53ef\uff0c\u8c22\u7edd\u8f6c\u8f7d\uff01<\/strong> <\/p>\n","protected":false},"excerpt":{"rendered":"\u5149\u50ac\u5316\u9886\u57df\u7684&#8221;\u5927\u54e5\u5927&#8221;MacMillan\u6559\u6388\u8fd9\u56de\u662f\u8981\u8fdb\u5165\u5316\u5b66\u751f\u7269\u5b66\u9886\u57df\u7684\u8282\u594f\uff1f Decarboxylative alkylation for site-selective\u00a0bioconjugation of native proteins via oxidation\u00a0potentials Bloom, S.; Liu, C.; K\u00f6lmel, D.; Qiao, J.; Zhang, Y.; Poss, M.; Ewing, W.; MacMillan, D. Nat. Chem. DOI:\u00a010.1038\/NCHEM.2888 \u8fd9\u4e00\u6b21\u4ed6\u4eec\u628a\u5149\u50ac\u5316\u5e94\u7528\u5728\u4e86\u5c0f\u5206\u5b50\u53cd\u5e94\u65b9\u6cd5\u5b66\u5f00\u53d1\u7684\u4eba\u6765\u8bf4\u6bd4\u8f83\u5bb9\u6613\u4e0a\u624b\u7684bioconjugation\u4e0a\uff08\u7eaf\u5c5e\u4e2a\u4eba\u81c6\u65ad\uff09\u3002 bioconjugation\u662f\u4ec0\u4e48\uff1f\u653e\u5fc3\uff0c\u8bf7\u6233\u6211\uff0cchem-station\u65e9\u5c31\u6709\u8be5\u8bcd\u6761\u7684\u8be6\u7ec6\u6587\u7ae0\u4ecb\u7ecd\u3002 Bioconjugation\u7684\u5fc5\u8981\u6761\u4ef6\u6709 bioconjugation\u662f\u4ee5\u591a\u80bd\/\u86cb\u767d\u8d28\u4e3a\u5e95\u7269\uff0c \u53cd\u5e94\u5fc5\u987b\u5728\u542b\u6c34\u4f53\u7cfb\u4e2d\u8fdb\u884c\u3002 \u4e00\u822c\u6765\u8bf4\u86cb\u767d\u8d28\u6eb6\u6db2\u65e0\u6cd5\u914d\u7f6e\u6210\u9ad8\u6d53\u5ea6\u7684\uff08\u6eb6\u89e3\u6027\u4f4e\uff09\uff0c\u6240\u4ee5\u53cd\u5e94\u5fc5\u987b\u80fd\u5728\u4f4e\u6d53\u5ea6\u6761\u4ef6\u4e0b\u4e5f\u80fd\u8fdb\u884c\u3002 \u6761\u4ef6\u5fc5\u987b\u6e29\u548c \uff08\u907f\u514d\u7834\u574f\u86cb\u767d\u8d28\u7684\u4e09\u7ea7\u7ed3\u6784\uff09 \u5fc5\u987b\u5177\u6709\u9ad8\u9009\u62e9\u6027\u3002(\u591a\u79cd\u5b98\u80fd\u56e2\u5171\u5b58) \u8fd9\u662f\u76f8\u5f53\u56f0\u96be\u7684\u6761\u4ef6\uff0c\u800cMacmillan\u7684\u65b9\u6cd5\u663e\u7136\u6ee1\u8db3\u4e86\u8fd9\u4e9b\u5fc5\u8981\u6761\u4ef6\u3002 &nbsp; \u6c34\u4e2d\u7684\u53cd\u5e94\u6027\u4e0e\u5b98\u80fd\u56e2\u9009\u62e9\u6027 \u5728\u8fd9\u6b21\u7684\u8bba\u6587\u4e2d\uff0c\u4ed6\u4eec\u4f7f\u7528\u5149\u50ac\u5316\u5242\u4ea7\u751f\u81ea\u7531\u57fa\u8131\u7fa7\u5e76\u8fdb\u800c\u8fdb\u884c\u5b98\u80fd\u56e2\u5316\u53cd\u5e94\u3002\u8fc4\u4eca\u4e3a\u6b62\uff0c\u5c31\u53cd\u5e94\u6027\u800c\u8a00\uff0c\u5df2\u7ecf\u6709\u62a5\u9053\u8868\u660e\u5177\u6709\u9ad8\u53cd\u5e94\u6027\u7684\u81ea\u7531\u57fa\u53cd\u5e94\u5728\u6c34\u4e2d\u7684\u5bf9\u86cb\u767d\u8d28\u4fee\u9970\u4e5f\u662f\u53ef\u884c\u7684[1]\u3001\u56e0\u6b64\uff0c\u672c\u53cd\u5e94\u4f53\u7cfb\u7528\u4e8ebioconjugation\u4e5f\u662f\u6bd4\u8f83\u201c\u987a\u5176\u81ea\u7136\u201d\u3002 \u81ea\u7531\u57fa\u53cd\u5e94\u5e94\u7528\u4e8eBioconjugation &nbsp; \u63a5\u4e0b\u6765\u662f\u9009\u62e9\u6027\u7684\u95ee\u9898\u3002\u8fd9\u6b21\u62a5\u9053\u7684\u8bba\u6587\u662fC\u672b\u7aef\u9009\u62e9\u6027\u7684\u8131\u7fa7\u578b\u5b98\u80fd\u56e2\u5316\u53cd\u5e94\u3002\u5728\u86cb\u767d\u8d28\u4e2d\u9664\u4e86\u78b3\u672b\u7aef\u4ee5\u5916\uff0c\u5929\u51ac\u6c28\u9178\u548c\u8c37\u6c28\u9178\u7684\u4fa7\u94fe\u4e0a\u4e5f\u5e26\u6709\u7fa7\u9178\u57fa\u56e2\uff0c\u7136\u800c\uff0c\u5728\u82ef\u53cd\u5e94\u4e2d\uff0c\u76f8\u5bf9\u4e0e\u5929\u51ac\u6c28\u9178\u4e0e\u8c37\u6c28\u9178\u7684\u4fa7\u94fe\u7fa7\u9178\u57fa\u56e2\u6765\u8bf4\uff0c\u53cd\u5e94\u9009\u62e9\u6027\u7684\u53d1\u751f\u5728\u5fae\u91cf\u7684\u7684C\u672b\u7aef\u7684\u7fa7\u9178\uff08C-terminalcarboxylate\uff09\u4e0a\u3002\u78b3\u672b\u7aef\u7684\u7fa7\u9178\u8131\u7fa7\u540e\u5f62\u6210\u7684\u81ea\u7531\u57fa\u7531\u4e8e\u90bb\u4f4d\u6709\u6742\u539f\u5b50\uff08\u6c2e\u539f\u5b50\uff09\u7684\u5b58\u5728\uff0c\u56e0\u6b64\u5b83\u6bd4\u7531\u5929\u51ac\u6c28\u9178\u6216\u8c37\u6c28\u9178\u4fa7\u94fe\u4ea7\u751f\u7684\u81ea\u7531\u57fa\u66f4\u7a33\u5b9a\u3002\u8fd9\u5c31\u662f\u4e3a\u4ec0\u4e48C\u672b\u7aef\u9009\u62e9\u6027\u7684\u53d1\u751f\u8131\u7fa7\u53cd\u5e94\u7684\u7f18\u7531\u3002 \u53cd\u5e94\u7684\u9009\u62e9\u6027\uff1a\u4fa7\u94fe\u7684\u7fa7\u9178 vs C\u672b\u7aef\u7684\u7fa7\u9178 \u5149\u50ac\u5316\u5242\u7684\u63a2\u8ba8 \u5728\u5927\u81f4\u4e86\u89e3\u4e3a\u4f55\u5728\u78b3\u672b\u7aef\u7684\u7fa7\u9178\u4e0a\u53d1\u751f\u9009\u62e9\u6027\u53cd\u5e94\u540e\u3002\u4e5f\u5c31\u662f\u8bf4MacMillan\u4ed6\u4eec\u628a\u4ee5\u524d\u7528\u5728\u5c0f\u5206\u5b50\u4e2d\u7684\u65b9\u6cd5\u5b66\u7528\u5230\u86cb\u767d\u8d28\u4e0a\u5c31\u641e\u5b9a\u4e86\uff0c\u7136\u800c\uff0c\u5b9e\u9645\u4e0a\u5e76\u975e\u8fd9\u4e48\u7b80\u5355\u3002 \u4f5c\u8005\u53d1\u73b0\uff0c\u672c\u53cd\u5e94\u5728\u5e38\u7528\u7684\u53cd\u5e94\u4f53\u7cfb-\u6c34\u6eb6\u6027\u954d\u50ac\u5316\u5242\u4e0e\u6709\u673a\u5149\u654f\u8bd5\u5242\u7684\u6761\u4ef6\u4e0b\uff0c\u53cd\u5e94\u5e76\u4e0d\u80fd\u8fdb\u884c\u3002\u56e0\u6b64\uff0c\u4ed6\u4eec\u7b5b\u9009\u540e\u9009\u62e9flavin\uff08\u9ec4\u7d20\u884d\u751f\u7269\uff09\u4f5c\u4e3aSET\u50ac\u5316\u5242\uff0c\u56e0\u4e3a\u6709\u62a5\u9053\u8868\u660eflavin\u53ef\u4ee5\u50ac\u5316\u03b1-\u53d6\u4ee3\u7684\u4e59\u9178\u916f\u7684\u8131\u7fa7\u53cd\u5e94\uff0c\u4f46\u662f\u5b83\u662f\u4e8c\u7535\u5b50\u8f6c\u79fb\u53cd\u5e94\u3002\u770b\u4f3cflavin\u5e76\u4e0d\u9002\u5408\u7528\u5728\u4f46\u7535\u5b50\u79fb\u52a8\u53cd\u5e94\uff08SET\uff09\u4e0a\uff0c\u7136\u800c\uff0c\u771f\u6b63\u8bd5\u4e86\u540e\u53d1\u73b0\u6548\u679c\u975e\u5e38\u597d\u3002 \u53cd\u5e94\u673a\u7406: flavin\u4f5c\u4e3aSET\u50ac\u5316\u5242\u4f5c\u7528 Limitation\u662f\uff1f \u4e00\u822c\u5bf9\u4e8e\u81ea\u7531\u57fa\u53cd\u5e94\u6765\u8bf4\u6bd4\u8f83\u5728\u610f\u7684\u7b2c\u4e00\u611f\u89c9\u5c31\u662f\u5b98\u80fd\u56e2\u7684\u517c\u5bb9\u6027\u3002\u5728\u62e5\u6709\u4ee5\u4e0b\u6c28\u57fa\u9178\u7684\u86cb\u767d\u8d28\u4e2d\uff0c Lys\u4e0eHis\u2192\u9178\u6027\u6761\u4ef6\u4e0b\uff0c\u53cd\u5e94\u80fd\u591f\u987a\u5229\u8fdb\u884c\u3002 Tyr\u2192\u4f4e\u6d3b\u6027\u7684flavin\u50ac\u5316\u5242\u6761\u4ef6\u4e0b\u53cd\u5e94\u53ef\u4ee5\u987a\u5229\u8fdb\u884c\uff0c\u4f46\u662f\u4ea7\u7387\u6bd4\u8f83\u4f4e\u3002Tyr\u5177\u6709\u6f5c\u5728\u7684\u8fd8\u539f\u6027\uff0c\u6240\u4ee5\u6bd4\u8f83\u5bb9\u6613\u88ab\u6c27\u5316\u3002 Cys\u3001Met\u3001Trp\u2192\u5177\u6709\u8fd9\u4e9b\u6c28\u57fa\u9178\u7684\u591a\u80bd\/\u86cb\u767d\u8d28\u7684\u5e95\u7269\u6ca1\u6709\u5728\u8bba\u6587\u4e2d\u51fa\u73b0\u3002\u5bf9\u4e8eCys\uff0c\u7531\u4e8e\u8fd9\u6b21\u53cd\u5e94\u4e2d\u7528\u5230\u4e86\u8fc8\u514b\u5c14\u53d7\u4f53\uff0c\u56e0\u6b64\u4f30\u8ba1\u8be5\u6761\u4ef6\u5f88\u96be\u9002\u7528\u4e8eCys\u3002\u5bf9\u4e8eMet\u4e0eTrp\uff0c\u786b\u919a\u548c\u5432\u54da\u7684\u6c27\u5316\u53cd\u5e94\u5e94\u8be5\u4f1a\u4f18\u5148\u8fdb\u884c\u3002 \u4e0a\u8ff0\u4ee5\u5916\u7684\u6c28\u57fa\u9178\u90fd\u9002\u7528\u4e8e\u672c\u6761\u4ef6\u3002 \u80f0\u5c9b\u7d20\u7684\u5b98\u80fd\u56e2\u5316 \u4f5c\u4e3a\u672c\u624b\u6cd5\u7684\u5e94\u7528\uff0c\u4f5c\u8005\u5c1d\u8bd5\u7740\u5e94\u7528\u4e8e\u80f0\u5c9b\u7d20\u7684\u5b98\u80fd\u5316\u3002\u4e5f\u5c31\u662f\u8bf4\uff0c\u8be5\u86cb\u767d\u8d28\u6709\u4e24\u5904\u5177\u6709\u78b3\u672b\u7aef\u7fa7\u9178\u7ed3\u6784\uff0c\u53e6\u5916\uff0c\u8fd9\u4e24\u6761\u80bd\u94fe\u542b\u6709\u56db\u4e2a\u8c37\u6c28\u9178\u6b8b\u57fa\u3002\u6b64\u5916\uff0c\u5728\u8fd9\u4e2a\u53cd\u5e94\u4f53\u7cfb\u4e2d\u6709\u672c\u53cd\u5e94\u4f53\u7cfb\u6bd4\u8f83\u96be\u641e\u5b9a\u7684\u56db\u4e2a\u916a\u6c28\u9178\u6b8b\u57fa\uff0c\u4e5f\u5c31\u662f\u8bf4\u80f0\u5c9b\u7d20\u5176\u5b9e\u662f\u4e00\u4e2a\u5177\u6709\u6311\u6218\u6027\u7684\u76ee\u6807\u5e95\u7269\u3002 \u4f46\u662f\uff0c\u5b9e\u9645\u5728\u5c1d\u8bd5\u540e\u53d1\u73b0\uff0cA-chain\u4f18\u5148\u53d1\u751f\u4e86C\u672b\u7aef\u7fa7\u9178\u7684\u5b98\u80fd\u56e2\u5316\u53cd\u5e94\u3002\u5f53\u7136\uff0c\u4e8c\u786b\u952e\uff0c\u8c37\u6c28\u9178\u7684\u7fa7\u9178\u548c\u5176\u4ed6\u6742\u539f\u5b50\u90fd\u88ab\u4fdd\u7559\u4e0b\u6765\uff0c\u5e76\u6ca1\u6709\u53d7\u5230\u5f71\u54cd\u3002 \u5e94\u7528\u4e8e\u80f0\u5c9b\u7d20 &nbsp; \u5bf9\u4e8eA-chain\u4f18\u5148\u4e8eB-chain\u53d1\u751f\u53cd\u5e94\uff0c\u4f5c\u8005\u7ed9\u51fa\u4e86\u4ee5\u4e0b\u4e24\u70b9\u53ef\u80fd\u7684\u7406\u7531\u3002 \u5176\u4e2d\u4e00\u70b9\u662f\u5355\u7eaf\u7684A-chain\u7684\u78b3\u672b\u7aef\u6bd4B-chain\u7684\u66f4\u5bb9\u6613\u88ab\u6c27\u5316\u3002 \u53e6\u4e00\u70b9\u4ed6\u4eec\u8ba4\u4e3a\u6709\u53ef\u80fd\u662f\uff0cA-chain\u7684\u78b3\u672b\u7aef\u8fd1\u65c1\u7684\u758f\u6c34\u8868\u9762\u66f4\u5bb9\u6613&#8221;\u805a\u96c6&#8221;\u5149\u50ac\u5316\u5242\uff0c\u901a\u8fc7\u8be5\u8fd1\u65c1\u6548\u679c\uff0cA-chain\u7684C\u672b\u7aef\u4f18\u5148\u53d1\u751f\u53cd\u5e94\u3002\u867d\u7136\u8fd9\u4e24\u4e2a\u53ef\u80fd\u6027\u5047\u8bf4\u5e76\u6ca1\u6709\u76f4\u63a5\u7684\u8bc1\u636e\uff0c\u4f46\u662fA-chain\u4e0eB-chain\u7684\u9009\u62e9\u6027\u5dee\u5f02\u8fd9\u4e00\u53d1\u73b0\u8fd8\u662f\u975e\u5e38\u6709\u8da3\u7684\u3002 &nbsp; \u7ed3\u5c3e \u5b8c\u7f8e\u7684bioconjugation\u7684\u65b9\u6cd5\u5b66\u8fd8\u5e76\u6ca1\u6709\u88ab\u5f00\u53d1\u51fa\u6765\uff0c\u9884\u8ba1\u8fd8\u6709\u5f88\u957f\u7684\u8def\u8981\u8d70\uff0c\u4f46\u4e0d\u53ef\u5426\u8ba4\uff0c\u8fd9\u4f1a\u662f\u4e00\u4e2a\u975e\u5e38\u70ed\u70b9\u7684\u8bfe\u9898\u3002 \u8fd9\u6b21\uff0cMacmillan\u7ec4\u4ec5\u4ec5\u662f\u5c1d\u8bd5\u5e94\u7528\u4e8ebioconjugation\uff0c\u800c\u672a\u6765\u6216\u8005\u662f\u5426\u76ee\u524d\u5df2\u7ecf\u5728\u5c1d\u8bd5\u5e94\u7528\u4e8ebioorthogonal\u53cd\u5e94\uff0c\u751a\u81f3\u662f\u6d3b\u7ec6\u80de\u4e2d\u5929\u7136\u86cb\u767d\u8d28\u7684\u6807\u8bb0\u7b49\u7b49\uff1f\u8ba9\u6211\u4eec\u62ed\u76ee\u4ee5\u5f85\u3002 \u53c2\u8003\u6587\u732e [1]Sato, S.; Nakamura, H. Angew. Chem., Int. Ed. 2013, 52, 8681. 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