{"id":18020,"date":"2018-04-24T09:35:35","date_gmt":"2018-04-24T01:35:35","guid":{"rendered":"\/\/www.gsbet888.com\/?p=18020"},"modified":"2019-08-02T18:51:57","modified_gmt":"2019-08-02T10:51:57","slug":"%e4%bb%85%e7%94%a8%e5%94%be%e6%b6%b2%e5%8d%b3%e5%8f%af%e6%a3%80%e6%b5%8b%ef%bc%81%ef%bc%9f-1-atto-mol%e4%bb%a5%e4%b8%8b%e7%9a%84%e8%b6%85%e9%ab%98%e7%81%b5%e6%95%8f%e5%ba%a6%e6%8a%97%e4%bd%93%e6%a3%80","status":"publish","type":"post","link":"\/\/www.gsbet888.com\/%e5%8c%96%e5%ad%a6%e6%9d%82%e8%ae%b0\/2018\/04\/%e4%bb%85%e7%94%a8%e5%94%be%e6%b6%b2%e5%8d%b3%e5%8f%af%e6%a3%80%e6%b5%8b%ef%bc%81%ef%bc%9f-1-atto-mol%e4%bb%a5%e4%b8%8b%e7%9a%84%e8%b6%85%e9%ab%98%e7%81%b5%e6%95%8f%e5%ba%a6%e6%8a%97%e4%bd%93%e6%a3%80.html","title":{"rendered":"\u4ec5\u7528\u553e\u6db2\u5373\u53ef\u68c0\u6d4b\uff01\uff1f 1 atto mol\u4ee5\u4e0b\u7684\u8d85\u9ad8\u7075\u654f\u5ea6\u6297\u4f53\u68c0\u6d4b\u65b9\u6cd5"},"content":{"rendered":"

HIV\u65af\u5766\u798f\u5927\u5b66\u7684\u00a0Carolyn R. Bertozzi<\/a>\u6559\u6388\u8bfe\u9898\u7ec4\uff0c\u5f00\u53d1\u4e86\u7528\u4e8e\u9ad8\u7075\u654f\u5ea6\u68c0\u6d4b\u6297\u4f53\u7684\u6280\u672f\uff08Agglutination-PCR\uff1a\u51dd\u96c6PCR\u6cd5\uff09\u3001\u53ef\u4ee5\u6210\u529f\u5730\u68c0\u6d4b\u5230\u553e\u6db2\u4e2d\u75d5\u91cf\u5b58\u5728\u7684\u9ad8\u5ea6\u654f\u611f\u7684HIV\u6297\u4f53\u3002<\/strong><\/p>\n

\u201cAntibody detection by agglutination\u2013PCR (ADAP) enables early diagnosis of HIV infection by oral fluid analysis\u201d<\/p>\n

Tsai, C. T.; Robinson, P. V.; Cortez, F. J.; Elma, M. L. B.; Seftel, D.; Pourmandi, N.; Pandori, M. W.; Bertozzi, C. R. Proc. Natl. Acad. Sci. U. S. A. <\/em>2018<\/strong>. DOI: 10.1073\/pnas.1711004115<\/a><\/p><\/blockquote>\n

\uff11. HIV\u662f\uff1f<\/h4>\n

\"\"<\/p>\n

\uff08\u5f15\u7528\u81ea\uff1a\u7f8e\u56fd\u536b\u751f\u548c\u4eba\u7c7b\u670d\u52a1\u90e8 AIDSinfo<\/em><\/a>\u3002\uff09<\/p>\n

HIV<\/strong>\uff08human immunodeficiency virus<\/strong>\uff09<\/strong>\u662f\u4e00<\/strong>\u79cd\u611f\u67d3\u4eba\u4f53\u514d\u75ab\u7ec6\u80de\u7684\u75c5\u6bd2\u3002\u968f\u7740\u827e\u6ecb\u75c5\u6bd2\u5bf9\u514d\u75ab\u7cfb\u7edf\u7684\u7834\u574f\uff0c\u9010\u6e10\u4ece\u75c5\u6bd2\u611f\u67d3\u5230\u8f6c\u53d8\u6210\u4e3a\u827e\u6ecb\u75c5\u7684\u72b6\u6001\uff0c\u827e\u6ecb\u75c5\u901a\u5e38\u4f1a\u4f7f\u4eba\u4f53\u4e27\u5931\u5bf9\u611f\u67d3\u6027\u75c5\u539f\u4f53\u7684\u62b5\u6297\u529b\u5e76\u5bfc\u81f4\u5404\u79cd\u75be\u75c5\u3002\u73b0\u5728\uff0c\u5bf9\u4e8e\u827e\u6ecb\u75c5\u7684\u6cbb\u7597\u836f\u7269\u7684\u7814\u53d1\u6b63\u5728\u4e0d\u65ad\u53d6\u5f97\u8fdb\u5c55\uff0c\u5e76\u4e0d\u50cf\u8fc7\u53bb\u90a3\u6837\u201c\u827e\u6ecb\u75c5=\u81f4\u547d\u75be\u75c5\u201d\uff0c\u4f46\u662f\u7531\u4e8e\u827e\u6ecb\u75c5\u6bd2\u7684\u6f5c\u4f0f\u671f\u957f\u8fbe5\u81f310\u5e74\uff0c\u5373\u4f7f\u6709\u65f6\u5019\u611f\u67d3\u4e86\u800c\u4e0d\u77e5\uff0c\u56e0\u6b64\u4f20\u67d3\u7ed9\u5176\u4ed6\u4eba\u7684\u98ce\u9669\u8fd8\u662f\u5f88\u9ad8\u3002\u56e0\u6b64\uff0c\u5bf9\u827e\u6ecb\u75c5\u6bd2\u7684\u65e9\u671f\u68c0\u6d4b\u5c31\u663e\u5f97\u5c24\u4e3a\u91cd\u8981\u3002<\/p>\n

\u90a3\u4e48\uff0c\u5982\u4f55\u68c0\u67e5HIV\u611f\u67d3\u7684\u5b58\u5728\u4e0e\u5426\uff0c\u76ee\u524d\u7684\u4e3b\u6d41\u68c0\u6d4b\u65b9\u6cd5\u662f\u9a8c\u8840<\/strong>\u3002\u5f53\u611f\u67d3\u827e\u6ecb\u75c5\u75c5\u6bd2\u65f6\uff0c\u4f53\u5185\u4f1a\u4ea7\u751f\u6297\u4f53\uff0c\u56e0\u6b64\u53ef\u4ee5\u901a\u8fc7\u68c0\u67e5\u8840\u6db2\u6837\u672c\u4e2d\u6240\u542b\u7684\u827e\u6ecb\u75c5\u6bd2\u6297\u4f53\u7684\u6570\u91cf<\/span>\u6765\u67e5\u770b\u662f\u5426\u611f\u67d3\u4e86\u827e\u6ecb\u75c5\u6bd2\u3002\u7136\u800c\uff0c\u5bf9\u4e8e\u5927\u4f17\u6765\u8bf4\uff0c\u8840\u6db2\u68c0\u67e5\u6bd4\u76ae\u80a4\u8d34\u7247\u68c0\u6d4b\u548c\u553e\u6db2\u68c0\u6d4b\u66f4\u654f\u611f\uff0c\u4e0d\u7ba1\u4ece\u64cd\u4f5c\u4e0a\u8fd8\u662f\u4e2a\u4eba\u5fc3\u7406\u4e0a\u90fd\u76f8\u5bf9\u7684\u96be\u63a5\u53d7\u4e00\u70b9\u3002\u5bf9\u4e8e\u975e\u6d32\u8fd9\u79cd\u533b\u7597\u73af\u5883\u6bd4\u8f83\u843d\u540e\u7684\u5730\u533a\uff0c\u827e\u6ecb\u75c5\u867d\u7136\u4ecd\u7136\u662f\u5bfc\u81f4\u6b7b\u4ea1\u7684\u4e3b\u8981\u539f\u56e0\u4e4b\u4e00\uff0c\u4f46\u662f\u5728\u536b\u751f\u63a7\u5236\u4e0d\u8db3\u7684\u73af\u5883\u4e2d\u4f7f\u7528\u6ce8\u5c04\u5668\u8fdb\u884c\u8840\u6db2\u68c0\u6d4b\u662f\u975e\u5e38\u6709\u98ce\u9669\u7684\u3002<\/p>\n

\u5982\u679c\u80fd\u591f\u5728\u553e\u6db2\u4e2d\u68c0\u6d4bHIV\u6297\u4f53\uff0c\u90a3\u4e48HIV\u7684\u68c0\u6d4b\u5c31\u53ef\u4ee5\u53d8\u5f97\u66f4\u52a0\u5b89\u5168\uff0c\u65b9\u4fbf\u3002\u4f46\u662f\uff0c\u7531\u4e8e\u553e\u6db2\u4e2d\u4ec5\u5b58\u5728\u75d5\u91cf\u7684HIV\u6297\u4f53\uff0c\u56e0\u6b64\u96be\u4ee5\u901a\u8fc7\u9176\u514d\u75ab\u6d4b\u5b9a\uff08EIA\uff09\u7b49\u5e38\u89c4\u65b9\u6cd5\u8fdb\u884c\u68c0\u6d4b\u3002<\/p>\n

\u56e0\u6b64\uff0cBertozzi\u6559\u6388\u53ca\u5176\u540c\u4e8b\u5f00\u53d1\u4e86\u4e00\u79cd\u79f0\u4e3a\u51dd\u96c6 – \u805a\u5408\u9176\u94fe\u53cd\u5e94\uff08agglutination PCR\uff09\u7684\u6280\u672f[1]\uff0c\u8be5\u6280\u672f\u7ed3\u5408\u4e86\u6297\u4f53\u51dd\u96c6\u53cd\u5e94\u548c\u901a\u8fc7PCR\u6269\u589eDNA\u4ee5\u68c0\u6d4b\u5fae\u5f31\u5b58\u5728\u4e8e\u553e\u6db2\u4e2d\u7684HIV\u6297\u4f53\u3002<\/p>\n

\uff12.\u00a0Agglutination\u2013PCR\u6cd5\u7684\u673a\u5236<\/h4>\n

\u6d4b\u5b9a\u624b\u6cd5\u5982\u4e0b\u6240\u793a\uff08\u56fe1b, c\uff09<\/p>\n

    \n
  1. \u5236\u5907\u4e0e\u6297\u539fHIV\u86cb\u767d\u8d28\u7684\u7ed3\u5408\u7684\u4e00\u6761DNA\u94fe\uff0c\u5f62\u6210\u6297\u539f-DNA\u7ed3\u5408\u4f53\uff08\u56fe1b\uff09\u3002<\/li>\n
  2. \u4e0e\u60f3\u8981\u6d4b\u5b9a\u6297\u4f53\u91cf\u7684\u6837\u54c1\u6df7\u5408\u3002\u2192\u00a0\u6297\u539f\u4e0e\u6297\u4f53\u53cd\u5e94\u3002<\/strong><\/li>\n
  3. \u52a0\u5165\u4ea4\u8054DNA\u548cDNA\u8fde\u63a5\u9176\u3002\u2192 \u5f62\u6210\u4e00\u6761DNA<\/strong>\u3002<\/strong><\/li>\n
  4. \u52a0\u5165\u5f15\u7269\u548cDNA\u805a\u5408\u9176\u3002\u2192DNA\u7684\u6269\u589e\u3002<\/strong><\/li>\n
  5. \u901a\u8fc7\u5b9a\u91cfPCR\u6cd5\u6d4b\u91cf\u6269\u589e\u7684DNA\u7684\u91cf\u3002<\/li>\n<\/ol>\n

    \"\"<\/p>\n

    \u56fe1. Agglutination\u2013PCR\u6cd5\u7684\u673a\u5236\u3002<\/p>\n

    \u5728\u8be5\u65b9\u6cd5\u4e2d\uff0c\u901a\u8fc7\u4ea4\u8054DNA\u548cDNA\u8fde\u63a5\u9176\u7684\u4f5c\u7528\u4fee\u590d\u4e86\u57285’\u4fa7\u548c3’\u4fa7\u5206\u6210\u4e00\u534a\u7684DNA\uff08\u56fe1b\uff09\uff0c\u4ece\u800c\u5f62\u6210\u53ef\u6269\u589e\u7684\u5168\u957fDNA\u3002\u534a\u6bb5DNA\uff0c\u56e0\u4e3a\u53ea\u6709\u4e00\u4e2a\u5f15\u7269\u7ed3\u5408\u4f4d\u70b9\uff0cDNA\u4e0d\u4f1a\u88ab\u6269\u589e\u3002\u501f\u52a9\u5b58\u5728\u4e8e\u6d4b\u91cf\u6837\u54c1\u4e2d\u7684\u6297\u4f53\uff0c\u4f7f\u5f97\u6297\u539f-DNA\u5f7c\u6b64\u53d1\u751f\u4ea4\u8054\u3001\u7136\u540e\u4ec5\u4ec5\u5bf9\u4e8e\u5f62\u6210\u7684full-size\u7684DNA\u901a\u8fc7PCR\u8fdb\u884cDNA\u6269\u589e\u3002<\/p>\n

    3. \u4e34\u5e8a\u6837\u672c\u7684HIV\u6297\u4f53\u6d4b\u5b9a<\/h4>\n

    \u9996\u5148\uff0c\u5c06\u7eaf\u5316\u7684HIV\u6297\u4f53\u7a00\u91ca\u81f3\u5404\u79cd\u6d53\u5ea6\u5e76\u8fdb\u884c\u6d4b\u91cf\uff0c\u5bf9\u4e8e\u5404\u79cdHIV\u86cb\u767d\uff08p24\uff0cgp41\uff0cgp160\uff09\u4f5c\u4e3a\u6297\u539f\u8fdb\u884c\u6297\u539f-\u6297\u4f53\u53cd\u5e94\uff0c\u53d1\u73b0\u53ea\u9700\u8981110, 880, 550 zeptomol \uff0810-21<\/sup>mol\uff09\u7684\u7075\u654f\u5ea6\u5c31\u80fd\u68c0\u6d4b\u786e\u8ba4\u3002<\/p>\n

    \u7ee7\u800c\uff0c\u4f7f\u7528\u771f\u5b9e\u7684\u553e\u6db2\u6837\u672c\u8fdb\u884c\u4e86HIV\u6297\u4f53\u6d4b\u91cf\u3002\u56fe2\u3001\u662f\u4f7f\u7528\u5404\u79cdHIV\u86cb\u767d\uff08p24, gp41, gp120, gp160\uff09\u4f5c\u4e3a\u6297\u539f\u8fdb\u884c\u5b9a\u91cfPCR\uff08qPCR\uff09\u7684\u7ed3\u679c\u3002\u5728qPCR\u4e2d\uff0cCt\u503c\u610f\u5473\u7740DNA\u8fbe\u5230\u4e00\u5b9a\u91cf\u7684\u6269\u589e\u9700\u8981\u7684\u5faa\u73af\u6b21\u6570\u3002\u8fd9\u91cc\uff0cy\u8f74\u8868\u793a\u7a7a\u767dCt\u4e0e\u6d4b\u5b9a\u6837\u54c1\u7684\u5dee\u503c\uff08Ct\uff09\uff0c\u503c\u8d8a\u5927\u8868\u793aHIV\u6297\u4f53\u68c0\u6d4b\u91cf\u8d8a\u9ad8\u3002\u4ece\u56fe\u4e2d\u53ef\u4ee5\u770b\u51fa\uff0c\u6bcf\u4e2aHIV\u86cb\u767d\u4e2dHIV\u9633\u6027\u548c\u9634\u6027\u6837\u672c\u4e4b\u95f4\u5b58\u5728\u660e\u663e\u7684\u5dee\u5f02\uff08P <0.05\uff09\u3002<\/p>\n

    \"\"<\/p>\n

    \u56fe2. \u901a\u8fc7Agglutination\u2013PCR \u8fdb\u884c\u4e34\u5e8a\u6837\u672c\u7684HIV\u6297\u4f53\u6d4b\u5b9a\u3002\uff08\u5f15\u7528\u81ea\u8bba\u6587\uff09<\/p>\n

    \u53e6\u5916\uff0c\u5bf9\u4e8e\u4f7f\u7528\u73b0\u6709\u7684\u624b\u6cd5\uff08EIA\u6cd5\uff09\u65e0\u6cd5\u6d4b\u51fa\u662f\u5426\u542b\u6709HIV\u6297\u4f53\u7684\u553e\u6db2\u6837\u672c\uff0c\u901a\u8fc7\u4f7f\u7528Agglutination\u2013PCR\u6cd5\u8fdb\u884c\u4e86\u6d4b\u5b9a\u30028\u4e2a\u6837\u672c\u8fdb\u884c\u6d4b\u91cf\u540e\u53d1\u73b06\u4e2a\u663e\u793a\u51fa\u4e86\u9633\u6027\u3002\u5176\u4e2d\u5bf9\u5bf9\u5e94\u7684\u4e00\u4e2a\u8840\u6db2\u6837\u672c\u8fdb\u884c\u8840\u6db2\u6297\u4f53\u6d4b\u5b9a\u9a8c\u8bc1\u540e\u53d1\u73b0\uff0c\u4e0eAgglutination\u2013PCR\u6cd5\u7ed3\u679c\u4e00\u6837\u663e\u793a\u51fa\u4e86\u9633\u6027\u3002\u56e0\u6b64\uff0c\u5229\u7528\u8be5\u65b0\u624b\u6cd5\u5f88\u6709\u53ef\u80fd\u5b9e\u73b0\u553e\u6db2\u4e2dHIV\u611f\u67d3\u7684\u65e9\u671f\u8bca\u65ad\u3002<\/p>\n

     <\/p>\n

    4. \u6587\u672b<\/h4>\n

    \u8fd9\u6b21\u4ecb\u7ecd\u7684Agglutination\u2013PCR\u6cd5\u3001\u53ea\u9700\u89811 \u00b5L\u7684\u6837\u672c\uff0c\u5c31\u53ef\u4ee5\u68c0\u6d4b\u5230zepto\uff0810-21<\/sup>\uff09\u301catto\uff0810-18<\/sup>\uff09mol\u7684\u6297\u4f53<\/strong>\u3002\u6297\u4f53\u68c0\u6d4b\u4e0d\u4ec5\u4ec5\u5bf9\u4e8eHIV\u611f\u67d3\uff0c\u5bf9\u4e8eI\u578b\u7cd6\u5c3f\u75c5\uff0c\u7532\u72b6\u817a\u764c\u7b49\u7684\u8bca\u65ad\u4e5f\u662f\u81f3\u5173\u91cd\u8981\u7684\uff0c\u56e0\u6b64\u5f88\u671f\u5f85\u8fdb\u4e00\u6b65\u7684\u6539\u8fdb\u4e0e\u5b9e\u73b0\u4e34\u5e8a\u5e94\u7528\u3002<\/p>\n

    \u53c2\u8003\u6587\u732e<\/h4>\n
      \n
    1. Tsai, C.-t.; Robinson, P. V.; Spencer, C. A.; Bertozzi, C. R. ACS Cent. Sci. <\/em>2016,<\/strong> 2<\/em>, 139.\u00a0 DOI: 10.1021\/acscentsci.5b00340<\/a><\/li>\n<\/ol>\n

      \u672c\u6587\u7248\u6743\u5c5e\u4e8e\u00a0Chem-Station\u5316\u5b66\u7a7a\u95f4<\/a>\uff0c<\/strong> \u6b22\u8fce\u70b9\u51fb\u6309\u94ae\u5206\u4eab\uff0c\u672a\u7ecf\u8bb8\u53ef\uff0c\u8c22\u7edd\u8f6c\u8f7d\uff01 <\/p>\n","protected":false},"excerpt":{"rendered":"HIV\u65af\u5766\u798f\u5927\u5b66\u7684\u00a0Carolyn R. Bertozzi\u6559\u6388\u8bfe\u9898\u7ec4\uff0c\u5f00\u53d1\u4e86\u7528\u4e8e\u9ad8\u7075\u654f\u5ea6\u68c0\u6d4b\u6297\u4f53\u7684\u6280\u672f\uff08Agglutination-PCR\uff1a\u51dd\u96c6PCR\u6cd5\uff09\u3001\u53ef\u4ee5\u6210\u529f\u5730\u68c0\u6d4b\u5230\u553e\u6db2\u4e2d\u75d5\u91cf\u5b58\u5728\u7684\u9ad8\u5ea6\u654f\u611f\u7684HIV\u6297\u4f53\u3002 \u201cAntibody detection by agglutination\u2013PCR (ADAP) enables early diagnosis of HIV infection by oral fluid analysis\u201d Tsai, C. T.; Robinson, P. V.; Cortez, F. J.; Elma, M. L. B.; Seftel, D.; Pourmandi, N.; Pandori, M. W.; Bertozzi, C. R. Proc. Natl. Acad. Sci. U. S. A. 2018. DOI: 10.1073\/pnas.1711004115 \uff11. HIV\u662f\uff1f \uff08\u5f15\u7528\u81ea\uff1a\u7f8e\u56fd\u536b\u751f\u548c\u4eba\u7c7b\u670d\u52a1\u90e8 AIDSinfo\u3002\uff09 HIV\uff08human immunodeficiency virus\uff09\u662f\u4e00\u79cd\u611f\u67d3\u4eba\u4f53\u514d\u75ab\u7ec6\u80de\u7684\u75c5\u6bd2\u3002\u968f\u7740\u827e\u6ecb\u75c5\u6bd2\u5bf9\u514d\u75ab\u7cfb\u7edf\u7684\u7834\u574f\uff0c\u9010\u6e10\u4ece\u75c5\u6bd2\u611f\u67d3\u5230\u8f6c\u53d8\u6210\u4e3a\u827e\u6ecb\u75c5\u7684\u72b6\u6001\uff0c\u827e\u6ecb\u75c5\u901a\u5e38\u4f1a\u4f7f\u4eba\u4f53\u4e27\u5931\u5bf9\u611f\u67d3\u6027\u75c5\u539f\u4f53\u7684\u62b5\u6297\u529b\u5e76\u5bfc\u81f4\u5404\u79cd\u75be\u75c5\u3002\u73b0\u5728\uff0c\u5bf9\u4e8e\u827e\u6ecb\u75c5\u7684\u6cbb\u7597\u836f\u7269\u7684\u7814\u53d1\u6b63\u5728\u4e0d\u65ad\u53d6\u5f97\u8fdb\u5c55\uff0c\u5e76\u4e0d\u50cf\u8fc7\u53bb\u90a3\u6837\u201c\u827e\u6ecb\u75c5=\u81f4\u547d\u75be\u75c5\u201d\uff0c\u4f46\u662f\u7531\u4e8e\u827e\u6ecb\u75c5\u6bd2\u7684\u6f5c\u4f0f\u671f\u957f\u8fbe5\u81f310\u5e74\uff0c\u5373\u4f7f\u6709\u65f6\u5019\u611f\u67d3\u4e86\u800c\u4e0d\u77e5\uff0c\u56e0\u6b64\u4f20\u67d3\u7ed9\u5176\u4ed6\u4eba\u7684\u98ce\u9669\u8fd8\u662f\u5f88\u9ad8\u3002\u56e0\u6b64\uff0c\u5bf9\u827e\u6ecb\u75c5\u6bd2\u7684\u65e9\u671f\u68c0\u6d4b\u5c31\u663e\u5f97\u5c24\u4e3a\u91cd\u8981\u3002 \u90a3\u4e48\uff0c\u5982\u4f55\u68c0\u67e5HIV\u611f\u67d3\u7684\u5b58\u5728\u4e0e\u5426\uff0c\u76ee\u524d\u7684\u4e3b\u6d41\u68c0\u6d4b\u65b9\u6cd5\u662f\u9a8c\u8840\u3002\u5f53\u611f\u67d3\u827e\u6ecb\u75c5\u75c5\u6bd2\u65f6\uff0c\u4f53\u5185\u4f1a\u4ea7\u751f\u6297\u4f53\uff0c\u56e0\u6b64\u53ef\u4ee5\u901a\u8fc7\u68c0\u67e5\u8840\u6db2\u6837\u672c\u4e2d\u6240\u542b\u7684\u827e\u6ecb\u75c5\u6bd2\u6297\u4f53\u7684\u6570\u91cf\u6765\u67e5\u770b\u662f\u5426\u611f\u67d3\u4e86\u827e\u6ecb\u75c5\u6bd2\u3002\u7136\u800c\uff0c\u5bf9\u4e8e\u5927\u4f17\u6765\u8bf4\uff0c\u8840\u6db2\u68c0\u67e5\u6bd4\u76ae\u80a4\u8d34\u7247\u68c0\u6d4b\u548c\u553e\u6db2\u68c0\u6d4b\u66f4\u654f\u611f\uff0c\u4e0d\u7ba1\u4ece\u64cd\u4f5c\u4e0a\u8fd8\u662f\u4e2a\u4eba\u5fc3\u7406\u4e0a\u90fd\u76f8\u5bf9\u7684\u96be\u63a5\u53d7\u4e00\u70b9\u3002\u5bf9\u4e8e\u975e\u6d32\u8fd9\u79cd\u533b\u7597\u73af\u5883\u6bd4\u8f83\u843d\u540e\u7684\u5730\u533a\uff0c\u827e\u6ecb\u75c5\u867d\u7136\u4ecd\u7136\u662f\u5bfc\u81f4\u6b7b\u4ea1\u7684\u4e3b\u8981\u539f\u56e0\u4e4b\u4e00\uff0c\u4f46\u662f\u5728\u536b\u751f\u63a7\u5236\u4e0d\u8db3\u7684\u73af\u5883\u4e2d\u4f7f\u7528\u6ce8\u5c04\u5668\u8fdb\u884c\u8840\u6db2\u68c0\u6d4b\u662f\u975e\u5e38\u6709\u98ce\u9669\u7684\u3002 \u5982\u679c\u80fd\u591f\u5728\u553e\u6db2\u4e2d\u68c0\u6d4bHIV\u6297\u4f53\uff0c\u90a3\u4e48HIV\u7684\u68c0\u6d4b\u5c31\u53ef\u4ee5\u53d8\u5f97\u66f4\u52a0\u5b89\u5168\uff0c\u65b9\u4fbf\u3002\u4f46\u662f\uff0c\u7531\u4e8e\u553e\u6db2\u4e2d\u4ec5\u5b58\u5728\u75d5\u91cf\u7684HIV\u6297\u4f53\uff0c\u56e0\u6b64\u96be\u4ee5\u901a\u8fc7\u9176\u514d\u75ab\u6d4b\u5b9a\uff08EIA\uff09\u7b49\u5e38\u89c4\u65b9\u6cd5\u8fdb\u884c\u68c0\u6d4b\u3002 \u56e0\u6b64\uff0cBertozzi\u6559\u6388\u53ca\u5176\u540c\u4e8b\u5f00\u53d1\u4e86\u4e00\u79cd\u79f0\u4e3a\u51dd\u96c6 – \u805a\u5408\u9176\u94fe\u53cd\u5e94\uff08agglutination PCR\uff09\u7684\u6280\u672f[1]\uff0c\u8be5\u6280\u672f\u7ed3\u5408\u4e86\u6297\u4f53\u51dd\u96c6\u53cd\u5e94\u548c\u901a\u8fc7PCR\u6269\u589eDNA\u4ee5\u68c0\u6d4b\u5fae\u5f31\u5b58\u5728\u4e8e\u553e\u6db2\u4e2d\u7684HIV\u6297\u4f53\u3002 \uff12.\u00a0Agglutination\u2013PCR\u6cd5\u7684\u673a\u5236 \u6d4b\u5b9a\u624b\u6cd5\u5982\u4e0b\u6240\u793a\uff08\u56fe1b, c\uff09 \u5236\u5907\u4e0e\u6297\u539fHIV\u86cb\u767d\u8d28\u7684\u7ed3\u5408\u7684\u4e00\u6761DNA\u94fe\uff0c\u5f62\u6210\u6297\u539f-DNA\u7ed3\u5408\u4f53\uff08\u56fe1b\uff09\u3002 \u4e0e\u60f3\u8981\u6d4b\u5b9a\u6297\u4f53\u91cf\u7684\u6837\u54c1\u6df7\u5408\u3002\u2192\u00a0\u6297\u539f\u4e0e\u6297\u4f53\u53cd\u5e94\u3002 \u52a0\u5165\u4ea4\u8054DNA\u548cDNA\u8fde\u63a5\u9176\u3002\u2192 \u5f62\u6210\u4e00\u6761DNA\u3002 \u52a0\u5165\u5f15\u7269\u548cDNA\u805a\u5408\u9176\u3002\u2192DNA\u7684\u6269\u589e\u3002 \u901a\u8fc7\u5b9a\u91cfPCR\u6cd5\u6d4b\u91cf\u6269\u589e\u7684DNA\u7684\u91cf\u3002 \u56fe1. 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V.; Spencer, C. A.; Bertozzi, C. R. 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