{"id":18495,"date":"2018-04-04T07:12:06","date_gmt":"2018-04-03T23:12:06","guid":{"rendered":"\/\/www.gsbet888.com\/?p=18495"},"modified":"2019-08-02T18:51:46","modified_gmt":"2019-08-02T10:51:46","slug":"%e6%b4%bb%e4%bd%93%e7%bb%84%e7%bb%87%e7%9a%84%e4%ba%ba%e5%b7%a5%e6%a0%87%e8%ae%b0%e3%80%8cagox-chemistry%e3%80%8d","status":"publish","type":"post","link":"\/\/www.gsbet888.com\/%e5%8c%96%e5%ad%a6%e6%9d%82%e8%ae%b0\/2018\/04\/%e6%b4%bb%e4%bd%93%e7%bb%84%e7%bb%87%e7%9a%84%e4%ba%ba%e5%b7%a5%e6%a0%87%e8%ae%b0%e3%80%8cagox-chemistry%e3%80%8d.html","title":{"rendered":"\u6d3b\u4f53\u7ec4\u7ec7\u7684\u4eba\u5de5\u6807\u8bb0\u300cAGOX Chemistry\u300d"},"content":{"rendered":"<p><strong>\u4eac\u90fd\u5927\u5b66\u30fb<a href=\"\/\/www.gsbet888.com\/chemists\/2016\/08\/post-10883.html\">\u6d5c\u5730\u683c<\/a>\u8bfe\u9898\u7ec4\u5229\u7528\u5421\u5576\u809f\uff08PyOx\uff09\u4f5c\u4e3a\u6709\u673a\u50ac\u5316\u5242\u548c\u9170\u57fa\u4f9b\u4f53<em>N<\/em>-\u9170\u57fa-<em>N<\/em>-\u70f7\u57fa\u78fa\u9170\u80fa\uff08NASA\uff09\u6210\u529f\u5730\u5f00\u53d1\u4e86\u9170\u5316\u86cb\u767d\u8d28\u4fee\u9970\u65b9\u6cd5\u3002\u5e76\u4e14\u8fdb\u4e00\u6b65\u7814\u7a76\u53d1\u73b0\uff0c\u8be5\u65b9\u6cd5\u8fd8\u80fd\u5e94\u7528\u4e8e\u5728\u8bd5\u7ba1\u5185\u5bf9\u6d3b\u4f53\u7ec6\u80de\u8fdb\u884c\u4fee\u9970\uff0c\u800c\u4e14\u9009\u62e9\u6027\u30fb\u7a33\u5b9a\u6027\u30fb\u6548\u7387\u90fd\u975e\u5e38\u597d\u3002<\/strong><\/p>\n<blockquote><p>\u201dAffinity-Guided Oxime Chemistry for Selective Protein Acylation in Live Tissue Systems\u201d<br \/>\nTamura, T.; Song, Z.; Amaike, K.; Lee, S.; Yin, S.; Kiyonaka, S.; Hamachi, I.* <em>J. Am. Chem. Soc.<\/em> <strong>2017<\/strong>, <em>139<\/em>, 14181\u201314191. DOI: <a href=\"http:\/\/dx.doi.org\/10.1021\/jacs.7b07339\">10.1021\/jacs.7b07339<\/a><\/p><\/blockquote>\n<h4>\u8bfe\u9898\u8bbe\u5b9a<\/h4>\n<p>\u5728\u4f53\u5185\u73af\u5883\u4e0b\uff0c\u5bf9\u86cb\u767d\u8d28\u7684\u9009\u62e9\u6027\u6807\u8bb0\u53ef\u4ee5\u6210\u4e3a\u7814\u7a76\u5b83\u4eec\u7684\u7ed3\u6784\uff0c\u529f\u80fd\uff0c\u884c\u4e3a\u7b49\u7684\u6709\u6548\u5730\u4e00\u79cd\u624b\u6bb5\u3002\u9009\u62e9\u6027\u7684\u6807\u8bb0\uff0c\u4ee5\u94dc\u50ac\u5316\u7684\u53e0\u6c2e-\u7094\u70c3\u7684\u73af\u5316\u53cd\u5e94\u6709\u8d77\u70b9\uff0c\u5f00\u53d1\u51fa\u4e86\u5f88\u591a\u5f88\u591a\u7c7b\u4f3c\u7684\u8fd0\u7528\u4e0d\u540c\u91d1\u5c5e\u50ac\u5316\u7684\u751f\u4f53\u6b63\u4ea4\u53cd\u5e94\u3002<br \/>\n\u7136\u800c\uff0c\u8fd9\u4e9b\u624b\u6cd5\u7ecf\u5e38\u5b58\u5728\u4ee5\u4e0b\u95ee\u9898\uff0c<\/p>\n<ul>\n<li>\u4f7f\u7528\u6d3b\u6027\u5f88\u9ad8\u7684\u8fc7\u6e21\u91d1\u5c5e\uff0c\u6bd2\u6027\u6bd4\u8f83\u9ad8\uff0c\u4e0d\u9002\u7528\u4e8e\u6d3b\u4f53\u7ec6\u80de\u3002<\/li>\n<li>\u9700\u8981\u8fd0\u7528\u57fa\u56e0\u5de5\u7a0b\u7684\u4e00\u4e9b\u65b9\u6cd5\u5bfc\u5165\u4e00\u4e9b\u975e\u5929\u7136\u7684\u7ed3\u6784\u4f5c\u4e3a\u53cd\u5e94\u7684\u57fa\u70b9\uff0c\u591a\u5c11\u56de\u5f71\u54cd\u5728\u751f\u7269\u4f53\u4e2d\u7684\u529f\u80fd\u3002<\/li>\n<\/ul>\n<h4>\u89e3\u51b3\u624b\u6cd5<\/h4>\n<p>\u4e3a\u4e86\u89e3\u51b3\u4e0a\u8ff0\u95ee\u9898\uff0c\u4f5c\u8005\u5f00\u53d1\u4e86\u4e00\u79cd\u53ef\u4ee5\u4e0e\u86cb\u767d\u8d28\u7279\u5f02\u6027\u914d\u4f4d\u7684\u5e26\u6709\u6709\u673a\u50ac\u5316\u5242\uff08DMAP\uff09\u57fa\u56e2\u7684\u914d\u4f53\u7ec4\u5408\uff0c\u7136\u540e\u5982\u4e0b\u56fe\u6240\u793a\uff0c\u9009\u62e9\u6027\u7684\u5728DMAP\u8fd1\u65c1\u7684\u86cb\u767d\u8d28\u4f4d\u70b9\u8fdb\u884c\u9170\u57fa\u5316\u4fee\u9970\u3001\u4f5c\u8005\u547d\u540d\u8fd9\u79cd\u65b9\u6cd5\u4e3a<strong>\u300caffinity-guided DMAP(AGD) chemistry\u300d<\/strong>[1]\u3002\u8be5case\u4e2d\uff0c\u4f5c\u8005\u4f7f\u7528\u786b\u916f\u4f5c\u4e3a\u9170\u57fa\u5316\u4f9b\u4f53\uff0c\u901a\u8fc7\u5bf9\u4e0e\u86cb\u767d\u8d28\u914d\u4f4d\u7684\u914d\u4f53\u4e0a\u7684DMAP\u57fa\u56e2\u8fdb\u884c\u4eb2\u6838\u8fdb\u653b\uff0c\u5f62\u6210\u6d3b\u6027\u9170\u57fa\u5421\u5576\u9393\u4e2d\u95f4\u4f53\u3002\u901a\u8fc7\u8fd9\u79cd\u624b\u6cd5\uff0c\u53cd\u5e94\u9009\u62e9\u6027\u7684\u53d1\u751f\u5728\u914d\u4f53\u8fd1\u65c1\u7684Lys\u4e0a\uff0c\u5b9e\u73b0\u4e86\u9009\u62e9\u6027\u7684\u86cb\u767d\u8d28\u6807\u8bb0\u3002<br \/>\n\u7136\u800c\uff0c\u6b21AGD\u6cd5\u8fd8\u5b58\u5728\u4ee5\u4e0b\u7f3a\u70b9\u3001\u4e3a\u4e86\u6539\u5584\u8fd9\u4e9b\u7f3a\u70b9\uff0c\u6709\u5fc5\u8981\u5f00\u53d1\u522b\u7684\u6709\u673a\u50ac\u5316\u5242\/\u9170\u57fa\u5316\u4f9b\u4f53\u7ec4\u5408\u3002<\/p>\n<ul>\n<li>\u4e3a\u4e86\u4fdd\u8bc1DMAP\u7684\u4eb2\u6838\u80fd\u529b\uff0c\u8be5\u53cd\u5e94\u9700\u8981\u518d\u78b1\u6027\u6761\u4ef6\u4e0b\u8fdb\u884c(pH &gt; 8)<\/li>\n<li>\u786b\u916f\u7684\u53cd\u5e94\u6027\u9ad8\u3001\u9700\u8981\u4f4e\u6e29\u8fdb\u884c\u53cd\u5e94\uff0c\u4e0d\u7136\u7684\u8bdd\u4f1a\u5f15\u8d77\u975e\u9009\u62e9\u6027\u7684\u9170\u57fa\u5316background\u526f\u53cd\u5e94\u3002<\/li>\n<li>\u786b\u916f\u5728\u4f53\u5185\u73af\u5883\u6761\u4ef6\u4e0b\u4f1a\u5206\u89e3\u3002<\/li>\n<\/ul>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-18497 size-full\" src=\"https:\/\/assets.www.gsbet888.com\/uploads\/2018\/03\/AGOX_1-e1521817504707.png\" alt=\"\" width=\"638\" height=\"431\" srcset=\"https:\/\/assets.www.gsbet888.com\/uploads\/2018\/03\/AGOX_1-e1521817504707.png 638w, https:\/\/assets.www.gsbet888.com\/uploads\/2018\/03\/AGOX_1-e1521817504707-300x203.png 300w\" sizes=\"(max-width: 638px) 100vw, 638px\" \/><\/p>\n<p>\u8fd9\u4e00\u6b21\u4ecb\u7ecd\u7684\u8bba\u6587\u4e2d\uff0c\u4f5c\u8005\u7b49\u4eba\u62a5\u9053\u4e86\u4f7f\u7528\u5421\u5576\u809f\uff08PyOx\uff09\u4f5c\u4e3a\u6709\u673a\u50ac\u5316\u5242\u3001<em>N<\/em>-\u9170\u57fa-<em>N<\/em>-\u70f7\u57fa\u78fa\u9170\u80fa\uff08NASA\uff09\u4f5c\u4e3a\u9170\u57fa\u4f9b\u4f53\u7684<strong>\u300caffinity-guided oxime (AGOX) chemistry\u300d<\/strong>\u3002<br \/>\nPyOx\u5177\u6709\u8f83\u5f3a\u7684\u78b1\u6027\u30fb\u4eb2\u6838\u6027\uff0c\u5e76\u4e14\u5728\u4e2d\u6027\u6761\u4ef6\u4e0b\u5177\u6709\u6c34\u89e3\u6d3b\u6027\uff08pH7.2\u300137\u2103\uff0c\u4ee5p-nitrophenylacetate\u4e3a\u5bf9\u8c61\uff0cPyOx\u6d3b\u6027\u4e3aDMAP\u76849\u500d\uff09\u3002<br \/>\n\u53e6\u4e00\u65b9\u9762NASA\u662f\u5df2\u77e5\u7684\u9170\u57fa\u5316\u4f9b\u4f53\uff0c\u5bb9\u6613\u53d7\u5230\u4eb2\u6838\u8fdb\u653b\uff0c\u8fdb\u573a\u7528\u5728\u591a\u80bd\u6545\u4e61\u5408\u6210\u7684C-\u672b\u7aef\u7684linker\u7684\u7ed3\u5408\u90e8[2]\u3002\u7531\u4e8e\u5176\u975e\u5929\u7136\u7684\u7ed3\u6784\uff0c\u56e0\u6b64\u4e0d\u60e7\u9176\u964d\u89e3\uff0c\u540c\u65f6\u78fa\u9170\u57fa\u90e8\u4f4d(R<sup>1<\/sup>)\u4e0e\u9170\u80fa\u90e8\u4f4d(R<sup>2<\/sup>)\u7684\u7ed3\u6784\u53ef\u4ee5tunning\u3002\u53e6\u5916\uff0c\u4e0e\u786b\u916f\u76f8\u6bd4\u4eb2\u7535\u5b50\u6027\u8981\u4f4e\uff0c\u53ef\u4ee5\u6709\u6548\u6291\u5236\u6d3b\u6027\u56fd\u5f3a\u5f15\u8d77\u7684background\u53cd\u5e94\u3002<\/p>\n<p>\u57fa\u672c\u4e0aconcept\u4e0eAGD\u4e00\u6837\u3002<\/p>\n<h4>\u8bbe\u60f3\u7684\u6709\u6548\u6027\u9a8c\u8bc1<\/h4>\n<p>\u4f5c\u8005\u901a\u8fc7\u4f7f\u7528PyOx\u548cNASA\u9170\u57fa\u4f9b\u4f53\u7684\u7ec4\u5408\uff0c\u4f7f\u5f97\u5404\u79cd\u86cb\u767d\u8d28\u4fee\u9970\u6210\u4e3a\u53ef\u80fd\u3002\u672c\u8bba\u6587\u4e2d\u7740\u91cd\u9700\u8981\u9a8c\u8bc1\u7684\u6709\u4ee5\u4e0b\u4e09\u70b9<\/p>\n<ol>\n<li><strong>\u53ea\u53d1\u751f\u901a\u8fc7\u914d\u4f53-PyOx\u50ac\u5316\u5242\u4ecb\u5bfc\u7684\u4fee\u9970\u53cd\u5e94\u3001\u4e0d\u53d1\u751fBackground\u53cd\u5e94<\/strong><\/li>\n<li><strong>\u53cd\u5e94\u9002\u7528\u6761\u4ef6\u4e2d\u6027\u30fb37\u2103<\/strong><\/li>\n<li><strong>\u5e94\u7528\u4e8e\u6d3b\u4f53\u7ec6\u80de\u7684\u53ef\u80fd\u6027\uff08\u7ec6\u80de\u819c\u4e0a\u7684\u86cb\u767d\u8d28\u4f5c\u4e3a\u53cd\u5e94\u9776\u70b9\uff09<\/strong><\/li>\n<\/ol>\n<p>\u800c\u4ee5\u4e0b\u662f\u5177\u4f53\u7684\u5b9e\u9a8c\u7ed3\u679c\u3002<\/p>\n<h5>\u2460FKBP12\u7684\u751f\u7269\u7d20\u4fee\u9970<\/h5>\n<p>\u4f5c\u8005\u8bbe\u8ba1\u4e86\u4e0eFKBP12\u914d\u4f4d\u7684\u914d\u4f53\u7531(SLF)\u4e0ePyOx\u8fde\u63a5\u7684\uff08SLF-PyOx\uff09\u5206\u5b50\u7ec4\u6210\uff0c\u4ee5\u6b64\u914d\u4f53\u4f5c\u4e3a\u50ac\u5316\u5242\uff0c\u52a0\u4e0aBt-NASA\u4f5c\u4e3a\u751f\u7269\u7d20\u4f9b\u4f53\uff0c\u5c1d\u8bd5\u5bf9\u86cb\u767d\u8d28\u7684Lys\u6b8b\u57fa\u8fdb\u884c\u751f\u7269\u7d20\u4fee\u9970\u3002\u5728\u5bf9NASA\u7ed3\u6784\u8fdb\u884c\u4e00\u5b9a\u7b5b\u9009\u540e\uff0c\u53d1\u73b0\u542b\u6709p-\u785d\u57fa\u53d6\u4ee3\u7684\u7ed3\u6784\u663e\u793a\u51fa\u5f88\u597d\u7684\u53cd\u5e94\u6d3b\u6027\u3002\u914d\u4f53\u8fd1\u65c1\u7684\u6b8b\u57fa\u6709\u4e24\u4e2a\u5206\u522b\u662fLys44, Lys52\u3001\u65e0\u8bba\u54ea\u4e2a\u6b8b\u57fa\u90fd\u53ef\u4ee5\u8fdb\u884c\u5b9a\u91cf\u7684\u4fee\u9970\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-18498 size-full\" src=\"https:\/\/assets.www.gsbet888.com\/uploads\/2018\/03\/AGOX_2-e1521817601769.png\" alt=\"\" width=\"633\" height=\"256\" srcset=\"https:\/\/assets.www.gsbet888.com\/uploads\/2018\/03\/AGOX_2-e1521817601769.png 633w, https:\/\/assets.www.gsbet888.com\/uploads\/2018\/03\/AGOX_2-e1521817601769-300x121.png 300w\" sizes=\"(max-width: 633px) 100vw, 633px\" \/><\/p>\n<p>\u540c\u65f6\uff0c\u4f5c\u8005\u8fd8\u53d1\u73b0<\/p>\n<ul>\n<li>\u914d\u4f53\u4e2d\u65e0PyOx\u7684\u8bdd\u4fee\u9970\u53cd\u5e94\u65e0\u6cd5\u8fdb\u884c<\/li>\n<li>\u5f53\u6dfb\u52a0\u7684SLF\u7ade\u4e89\u53cd\u5e94\u5242FK506\u540e\uff0c\u4e0d\u4f1a\u53d1\u751f\u5bf9SLF\u7684\u4fee\u9970\u53cd\u5e94<\/li>\n<li>Bt-NASA\u7684\u534a\u8870\u671f\u6bd4\u5176\u786b\u916f\u7248\u7684\u8981\u957f\u3002<\/li>\n<li>\u786b\u916f\u7248\u7684Bt-NASA\u4f1a\u53d1\u751fbackground\u53cd\u5e94<\/li>\n<\/ul>\n<p>\u4ee5\u4e0a\u56db\u70b9\u53ef\u4ee5\u8868\u660eAGOX Chemistry\u663e\u793a\u51fa\u4e86\u5176\u76f8\u5bf9\u4e8eAGD\u7684\u7279\u5f02\u6027\u3002<\/p>\n<h5>\u2461\u5bf9\u6d3b\u7ec6\u80de\u8868\u9762\u7684\u78b3\u9178\u9150\u9176(CA\u216b)\u8fdb\u884c\u8367\u5149\u6807\u8bb0<\/h5>\n<p>\u4f5c\u8005\u5c1d\u8bd5\u4e86\u5bf9\u751f\u7269\u819c\u8868\u9762\u86cb\u767d\u8d28CA\u216b\u8fdb\u884c\u8367\u5149\u6807\u8bb0\u3002\u5728\u8fd9\u4e2a\u53cd\u5e94\u4e2d\uff0c\u4e3a\u4e86\u8fdb\u4e00\u6b65\u63d0\u9ad8\u50ac\u5316\u5242\u6d3b\u6027\uff0c\u4f5c\u8005\u5728\u914d\u4f53(SA)\u4e0a\u5f15\u5165\u4e86\u4e24\u4e2aPyOx\u5206\u5b50\u5f62\u6210SA-diPyOx\u4f5c\u4e3a\u50ac\u5316\u5242\u4f7f\u7528\u3002<\/p>\n<p>SA-diPyOx\u4e0e\u8367\u5149\u6807\u8bb0\u8bd5\u5242\uff08FL-NASA\uff09\u5728pH7.2\u300137\u2103\u4e0b\u96e8\u7ec6\u80de\u8fdb\u884c\u53cd\u5e94\u3001SDS-PAGE\u76d1\u6d4b\u663e\u793a48\uff05\u7684CA\u57283\u5c0f\u65f6\u5185\u88ab\u8367\u5149\u6807\u8bb0\u3002\u4f5c\u4e3a\u5bf9\u7167\u5b9e\u9a8c\uff0c\u5229\u7528SA-triDMAP\uff0cAGD chemistry\u624b\u6cd5\uff0c\u540c\u6837\u8fdb\u884c\u6807\u8bb0\uff0c\u53d1\u73b0\u53d1\u751f\u4e86\u5927\u91cf\u7684\u975e\u7279\u5f02\u6027\u7684\u6807\u8bb0\u3002\u53e6\u5916\uff0c\u5f53\u52a0\u5165\u914d\u4f53-CAII\u7ed3\u5408\u963b\u65ad\u5242\uff08EZA\uff09\u540e\uff0c\u4fee\u9970\u53cd\u5e94\u5c31\u65e0\u6cd5\u8fdb\u884c\u4e86\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-18499 size-full\" src=\"https:\/\/assets.www.gsbet888.com\/uploads\/2018\/03\/AGOX_3-e1521817655459.png\" alt=\"\" width=\"650\" height=\"421\" srcset=\"https:\/\/assets.www.gsbet888.com\/uploads\/2018\/03\/AGOX_3-e1521817655459.png 650w, https:\/\/assets.www.gsbet888.com\/uploads\/2018\/03\/AGOX_3-e1521817655459-300x194.png 300w\" sizes=\"(max-width: 650px) 100vw, 650px\" \/>\u3000\u5229\u7528\u819c\u8868\u9762\u86cb\u767d\u8d28\u7684\u8367\u5149\u6807\u8bb0\u624b\u6cd5\uff0c\u4f5c\u8005\u8fd8\u901a\u8fc7\u6fc0\u5149\u892a\u8272\u5b9e\u9a8c\u7814\u7a76\u4e86\u5b83\u7684\u6269\u6563\u901f\u7387[3]\u3002 \u53d1\u73b0\u7ed3\u679c\u4e0e\u7528GFP\u878d\u5408\u86cb\u767d\u68c0\u6d4b\u7684\u503c\u4e00\u81f4\u3002<\/p>\n<h5>\u2462\u5bf9\u5c0f\u9f20\u8111\u7ec4\u7ec7\u4e2d\u7684AMPAR\u8fdb\u884c\u8367\u5149\u6807\u8bb0<\/h5>\n<p>\u505a\u7740\u51c6\u5907\u4e86\u5c0f\u9f20\u7684\u8111\u5207\u7247\u7ec4\u7ec7\u3001\u5bf9\u795e\u7ecf\u7ec6\u80de\u819c\u4e0a\u542b\u6709\u7684\u03b1-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)\u578b\u8c37\u6c28\u9178\u53d7\u4f53(AMPAR)\u8fdb\u884c\u4e86\u4fee\u9970\u53cd\u5e94\u3002\u8be5\u53cd\u5e94\u4e2d\u7684\u914d\u4f53\u7528\u4e86PFQX\u3002<br \/>\n\u5bf9\u4e8e\u8be5\u53cd\u5e94\uff0c\u4f5c\u8005\u4e5f\u5bf9\u6bd4\u4e86AGD\u7cfb(PFQX-triDMAP)\u4e0eAGOX\u7cfb(PFQX-diPyOx)\u3001\u901a\u8fc7SDS-PAGE\u5206\u6790\u8868\u660e\u53ea\u6709AGOX\u7cfb\u5b9e\u73b0\u4e86\u6709\u6548\u6807\u8bb0\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-18500 size-full\" src=\"https:\/\/assets.www.gsbet888.com\/uploads\/2018\/03\/AGOX_4-e1521817698973.png\" alt=\"\" width=\"650\" height=\"539\" srcset=\"https:\/\/assets.www.gsbet888.com\/uploads\/2018\/03\/AGOX_4-e1521817698973.png 650w, https:\/\/assets.www.gsbet888.com\/uploads\/2018\/03\/AGOX_4-e1521817698973-300x249.png 300w\" sizes=\"(max-width: 650px) 100vw, 650px\" \/><\/p>\n<h4>Comment<\/h4>\n<ul>\n<li>\u3000\u4e0e\u4ee5\u524d\u7684\u62a5\u9053\u76f8\u6bd4\uff0c\u8fd9\u6b21\u7684\u65b0\u65b9\u6cd5\u7684\u9009\u62e9\u6027\u5927\u5927\u7684\u63d0\u9ad8\u4e86\uff0c\u5e76\u4e14\u53ef\u4ee5\u9002\u7528\u4e8e\u6d3b\u7ec6\u80de\u4f53\u7cfb\u3002\u53e6\u5916\u53ef\u4ee5\u9009\u62e9\u7684\u914d\u4f53\u00b7\u9170\u57fa\u5316\u4f9b\u4f53\u7684\u79cd\u7c7b\u4e5f\u589e\u591a\u3002<\/li>\n<li>\u3000\u4e0b\u4e00\u4e2a\u8bfe\u9898\u5f88\u6709\u53ef\u80fd\u662f\u5e94\u7528\u4e8e<strong>\u6d3b\u7d30\u80de\u300c\u5185\u300d\u7684\u5316\u5b66\u53cd\u5e94<\/strong>\u3002\u8fd9\u4e00\u70b9\u4f5c\u8005\u4e5f\u5728\u6587\u4e2d\u8bf4\u4e86\uff0c\u5c1a\u672a\u8fbe\u6210\uff0c\u800c\u5f88\u6709\u53ef\u80fd\u662f\u56e0\u4e3aPyOx\u7684\u5421\u5576\u90e8\u7684\u9633\u79bb\u5b50\u6027\u4e0d\u5229\u4e8e\u900f\u8fc7\u7ec6\u80de\u819c\uff0c\u800c\u8fd9\u4e00\u96be\u70b9\u4e5f\u4f1a\u662f\u4e0b\u4e00\u4e2a\u9700\u8981\u6539\u5584\u7a81\u7834\u7684\u70b9\u3002<\/li>\n<\/ul>\n<h4>\u53c2\u8003\u6587\u732e<\/h4>\n<ol>\n<li>(a) Koshi, Y.; Nakata, E.; Miyagawa, M.; Tsukiji, S.; Ogawa, T.; Hamachi, I. <em>J. Am. Chem. Soc.<\/em> <strong>2008<\/strong>, <em>130<\/em>, 245. DOI: <a href=\"http:\/\/dx.doi.org\/10.1021\/ja075684q\">10.1021\/ja075684q<\/a> (b) Wang, H-x.; Koshi, Y.; Minato, D.; Nonaka, H.; Kiyonaka, S.; Mori, Y.; Tsukiji, S.; Hamachi, I. <em>J. Am. Chem. Soc.<\/em>\u00a0<strong>2011<\/strong>, <em>133<\/em>, 12220. DOI: <a href=\"http:\/\/dx.doi.org\/10.1021\/ja204422r\">10.1021\/ja204422r<\/a> (c) Hayashi, T.; Sun, Y.; Tamura, T.; Kuwata, K.; Song, Z.; Takaoka, Y.; Hamachi, I. <em>J. Am. Chem. Soc.\u00a0<\/em><strong>2013<\/strong>, <em>135<\/em>, 12252. DOI: <a href=\"http:\/\/dx.doi.org\/10.1021\/ja4043214\">10.1021\/ja4043214<\/a> (d) Hayashi, T.; Yasueda, Y.; Tamura, T.; Takaoka, Y.; Hamachi, I. <em>J. Am. Chem. Soc.<\/em> <strong>2015<\/strong>, <em>137<\/em>, 5372. DOI: <a href=\"http:\/\/dx.doi.org\/10.1021\/jacs.5b02867\">10.1021\/jacs.5b02867<\/a> (d) Song, Z.; Takaoka, Y.; Kioi, Y.; Komatsu, K.; Tamura, T.; Miki, T.; Hamachi, I. <em>Chem. Lett.<\/em> <strong>2015<\/strong>, <em>44<\/em>, 333. doi:<a href=\"http:\/\/dx.doi.org\/10.1246\/cl.141065\">10.1246\/cl.141065<\/a><\/li>\n<li>Heidler, P.; Link, A. <em>Bioorg. Med. Chem.<\/em> <strong>2005<\/strong>, <em>13<\/em>, 585. doi:<a href=\"http:\/\/dx.doi.org\/10.1016\/j.bmc.2004.10.045\">10.1016\/j.bmc.2004.10.045<\/a><\/li>\n<li>(a) Soumpasis, D. M. <em>Biophys. J.<\/em> <strong>1983<\/strong>, <em>41<\/em>, 95. (b) Sprague, B. L.; McNally, J. G. <em>Trends Cell Biol.<\/em> <strong>2005<\/strong>, <em>15<\/em>, 84. doi:<a href=\"http:\/\/dx.doi.org\/10.1016\/j.tcb.2004.12.001\">10.1016\/j.tcb.2004.12.001<\/a><\/li>\n<\/ol>\n<p>\u672c\u6587\u7248\u6743\u5c5e\u4e8e\u00a0<a href=\"\/\/www.gsbet888.com\/\">Chem-Station\u5316\u5b66\u7a7a\u95f4<\/a>\uff0c \u6b22\u8fce\u70b9\u51fb\u6309\u94ae\u5206\u4eab\uff0c\u672a\u7ecf\u8bb8\u53ef\uff0c\u8c22\u7edd\u8f6c\u8f7d\uff01 <\/p>\n","protected":false},"excerpt":{"rendered":"\u4eac\u90fd\u5927\u5b66\u30fb\u6d5c\u5730\u683c\u8bfe\u9898\u7ec4\u5229\u7528\u5421\u5576\u809f\uff08PyOx\uff09\u4f5c\u4e3a\u6709\u673a\u50ac\u5316\u5242\u548c\u9170\u57fa\u4f9b\u4f53N-\u9170\u57fa-N-\u70f7\u57fa\u78fa\u9170\u80fa\uff08NASA\uff09\u6210\u529f\u5730\u5f00\u53d1\u4e86\u9170\u5316\u86cb\u767d\u8d28\u4fee\u9970\u65b9\u6cd5\u3002\u5e76\u4e14\u8fdb\u4e00\u6b65\u7814\u7a76\u53d1\u73b0\uff0c\u8be5\u65b9\u6cd5\u8fd8\u80fd\u5e94\u7528\u4e8e\u5728\u8bd5\u7ba1\u5185\u5bf9\u6d3b\u4f53\u7ec6\u80de\u8fdb\u884c\u4fee\u9970\uff0c\u800c\u4e14\u9009\u62e9\u6027\u30fb\u7a33\u5b9a\u6027\u30fb\u6548\u7387\u90fd\u975e\u5e38\u597d\u3002 \u201dAffinity-Guided Oxime Chemistry for Selective Protein Acylation in Live Tissue Systems\u201d Tamura, T.; Song, Z.; Amaike, K.; Lee, S.; Yin, S.; Kiyonaka, S.; Hamachi, I.* J. Am. Chem. Soc. 2017, 139, 14181\u201314191. DOI: 10.1021\/jacs.7b07339 \u8bfe\u9898\u8bbe\u5b9a \u5728\u4f53\u5185\u73af\u5883\u4e0b\uff0c\u5bf9\u86cb\u767d\u8d28\u7684\u9009\u62e9\u6027\u6807\u8bb0\u53ef\u4ee5\u6210\u4e3a\u7814\u7a76\u5b83\u4eec\u7684\u7ed3\u6784\uff0c\u529f\u80fd\uff0c\u884c\u4e3a\u7b49\u7684\u6709\u6548\u5730\u4e00\u79cd\u624b\u6bb5\u3002\u9009\u62e9\u6027\u7684\u6807\u8bb0\uff0c\u4ee5\u94dc\u50ac\u5316\u7684\u53e0\u6c2e-\u7094\u70c3\u7684\u73af\u5316\u53cd\u5e94\u6709\u8d77\u70b9\uff0c\u5f00\u53d1\u51fa\u4e86\u5f88\u591a\u5f88\u591a\u7c7b\u4f3c\u7684\u8fd0\u7528\u4e0d\u540c\u91d1\u5c5e\u50ac\u5316\u7684\u751f\u4f53\u6b63\u4ea4\u53cd\u5e94\u3002 \u7136\u800c\uff0c\u8fd9\u4e9b\u624b\u6cd5\u7ecf\u5e38\u5b58\u5728\u4ee5\u4e0b\u95ee\u9898\uff0c \u4f7f\u7528\u6d3b\u6027\u5f88\u9ad8\u7684\u8fc7\u6e21\u91d1\u5c5e\uff0c\u6bd2\u6027\u6bd4\u8f83\u9ad8\uff0c\u4e0d\u9002\u7528\u4e8e\u6d3b\u4f53\u7ec6\u80de\u3002 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chemistry\u300d[1]\u3002\u8be5case\u4e2d\uff0c\u4f5c\u8005\u4f7f\u7528\u786b\u916f\u4f5c\u4e3a\u9170\u57fa\u5316\u4f9b\u4f53\uff0c\u901a\u8fc7\u5bf9\u4e0e\u86cb\u767d\u8d28\u914d\u4f4d\u7684\u914d\u4f53\u4e0a\u7684DMAP\u57fa\u56e2\u8fdb\u884c\u4eb2\u6838\u8fdb\u653b\uff0c\u5f62\u6210\u6d3b\u6027\u9170\u57fa\u5421\u5576\u9393\u4e2d\u95f4\u4f53\u3002\u901a\u8fc7\u8fd9\u79cd\u624b\u6cd5\uff0c\u53cd\u5e94\u9009\u62e9\u6027\u7684\u53d1\u751f\u5728\u914d\u4f53\u8fd1\u65c1\u7684Lys\u4e0a\uff0c\u5b9e\u73b0\u4e86\u9009\u62e9\u6027\u7684\u86cb\u767d\u8d28\u6807\u8bb0\u3002 \u7136\u800c\uff0c\u6b21AGD\u6cd5\u8fd8\u5b58\u5728\u4ee5\u4e0b\u7f3a\u70b9\u3001\u4e3a\u4e86\u6539\u5584\u8fd9\u4e9b\u7f3a\u70b9\uff0c\u6709\u5fc5\u8981\u5f00\u53d1\u522b\u7684\u6709\u673a\u50ac\u5316\u5242\/\u9170\u57fa\u5316\u4f9b\u4f53\u7ec4\u5408\u3002 \u4e3a\u4e86\u4fdd\u8bc1DMAP\u7684\u4eb2\u6838\u80fd\u529b\uff0c\u8be5\u53cd\u5e94\u9700\u8981\u518d\u78b1\u6027\u6761\u4ef6\u4e0b\u8fdb\u884c(pH &gt; 8) 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T.; Hamachi, I. J. Am. Chem. Soc. 2008, 130, 245. 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