{"id":19432,"date":"2018-06-18T06:10:44","date_gmt":"2018-06-17T22:10:44","guid":{"rendered":"\/\/www.gsbet888.com\/?p=19432"},"modified":"2019-08-02T18:51:30","modified_gmt":"2019-08-02T10:51:30","slug":"%e7%bb%bf%e8%89%b2%e8%8d%a7%e5%85%89%e8%9b%8b%e7%99%bd%e8%b4%a8-green-fluorescent-protein-gfp","status":"publish","type":"post","link":"\/\/www.gsbet888.com\/molecule\/2018\/06\/%e7%bb%bf%e8%89%b2%e8%8d%a7%e5%85%89%e8%9b%8b%e7%99%bd%e8%b4%a8-green-fluorescent-protein-gfp.html","title":{"rendered":"\u7eff\u8272\u8367\u5149\u86cb\u767d\u8d28 \/Green Fluorescent Protein (GFP)"},"content":{"rendered":"<p><strong>\u7eff\u8272\u8367\u5149\u86cb\u767d(Green Fluorescent Protein;GFP)<\/strong>\u3001\u662f\u4ece\u7ef4\u591a\u5229\u4e9a\u591a\u7ba1\u53d1\u5149\u6c34\u6bcd(<em>Aequorea victoria<\/em>)\u4e2d\u5206\u79bb\u51fa\u6765\u7684\u4e00\u79cd\u8367\u5149\u86cb\u767d\u3002<\/p>\n<h4>\u7528\u9014\u30fb\u5386\u53f2<\/h4>\n<p>\u8bfa\u5956\u5f97\u4e3b\u30fb\u4e0b\u6751\u8129\u7b49\u4eba\u57281962\u5e74\u4ece\u7ef4\u591a\u5229\u4e9a\u591a\u7ba1\u53d1\u5149\u6c34\u6bcd\u4e2d\u5206\u79bb\u30fb\u7cbe\u5236\u540e\u83b7\u5f97\u7684\u3002<sup>[1]<\/sup><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-19434\" src=\"https:\/\/assets.www.gsbet888.com\/uploads\/2018\/06\/jerryfish.jpg\" alt=\"\" width=\"200\" height=\"150\" \/><\/p>\n<div style=\"text-align: center\">(\u7167\u7247\uff1a\u5f15\u7528\u81ea<a href=\"http:\/\/ja.wikipedia.org\/wiki\/%E3%82%AA%E3%83%AF%E3%83%B3%E3%82%AF%E3%83%A9%E3%82%B2\">Wikipedia<\/a>)<\/div>\n<p>\u5206\u5b50\u91cf\u7ea6\u4e3a27 kDa\u3001\u7531238\u4e2a\u6c28\u57fa\u9178\u7ec4\u6210\u3002 \u4e09\u7ef4\u7ed3\u6784\u57281996\u5e74\u901a\u8fc7X\u5c04\u7ebf\u5355\u6676\u884d\u5c04\u89e3\u6790\u5b8c\u6210<sup>[2]<\/sup>\u3002 \u5982\u6807\u9898\u56fe\u6240\u793a\uff0c11\u4e2a\u03b2\u5355\u4f4d\u662f\u4ee5\u87ba\u65cb\u72b6\u8fdb\u884c\u7ec4\u7ec7\u5316\u7684\uff0c\u5706\u67f1\u72b6\u7ed3\u6784\u4ee5\u5305\u56f4\u53d1\u5149\u56e2\uff08\u767d\u8272\u90e8\u5206\uff09\u7684\u5f62\u5f0f\u7ec4\u88c5\u3002\u8be5\u5706\u67f1\u7b52\u72b6\u7ed3\u6784\u53ef\u4ee5\u4fdd\u62a4\u5185\u90e8\u7684\u53d1\u5149\u56e2\uff0c\u63d0\u9ad8\u5176\u53d1\u5149\u6548\u7387\u4e0e\u5bff\u547d\u3002<\/p>\n<p>\u5728\u7ef4\u591a\u5229\u4e9a\u591a\u7ba1\u53d1\u5149\u6c34\u6bcd\u7684\u4f53\u5185\u3001\u7eff\u8272\u8367\u5149\u86cb\u767d\u4e0e\u9499\u53cd\u5e94\u86cb\u767d\u6c34\u6bcd\u53d1\u5149\u86cb\u767d\uff08\u6c34\u6bcd\u53d1\u5149\u86cb\u767d\uff09\u7ed3\u5408\u53d1\u5149\u3002\u4e5f\u5c31\u662f\u8bf4\uff0c\u6c34\u6bcd\u53d1\u5149\u86cb\u767d\u9996\u5148\u5438\u6536\u80fd\u91cf\uff0cGFP\u63a5\u6536\u53d1\u5c04\u7684\u84dd\u8272\u8367\u5149\u5e76\u53d1\u51fa\u7eff\u5149\u3002<\/p>\n<p>GFP\u7684\u53d1\u5149\u4f53\u5438\u6536\u8fd1\u7d2b\u5916\u5149\uff08397nm\uff09\u5e76\u53d1\u51fa\u7eff\u8272\u53ef\u89c1\u5149\uff08509nm\uff09\u3002\u53d1\u5149\u7ed3\u6784\u7531Ser65-Tyr66-Gly67\u4e09\u4e2a\u6c28\u57fa\u9178\u6b8b\u57fa\u901a\u8fc7\u73af\u5316\u30fb\u6c27\u5316\uff0c\u81ea\u53d1\u5f62\u6210\u3002\u8be5\u73af\u5316\u53cd\u5e94\u4e00\u822c\u6765\u8bf4\u975e\u5e38\u96be\u8fdb\u884c\uff0c\u7136\u800c\u5728GFP\u4e2d\u5f62\u6210\u4e86\u4e00\u79cd\u7279\u6b8a\u7684\u6784\u8c61\uff0c\u56e0\u6b64\u80fd\u591f\u5f88\u5bb9\u6613\u8fdb\u884c\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-19435 size-full\" src=\"https:\/\/assets.www.gsbet888.com\/uploads\/2018\/06\/GFP_3.gif\" alt=\"\" width=\"366\" height=\"189\" \/><\/p>\n<p>\u540c\u6837\u5f0f\u8bfa\u8d1d\u5c14\u5956\u5f97\u4e3bChalfie\u30fbTsien\u7b49\u4eba\u53d1\u73b0\u4e86\u5229\u7528\u8be5\u53d1\u5149\u86cb\u767d\u8fdb\u884c\u8367\u5149\u6807\u8bb0\u7684\u65b9\u6cd5\uff0c\u56e0\u6b64\u76ee\u524d\u5316\u5b66\u751f\u7269\u5b66\u4e2d\u7684\u8367\u5149\u6807\u8bb0\u65b9\u6cd5\u51fa\u73b0\u4e86\u7206\u53d1\u6027\u7684\u53d1\u5c55\u3002<\/p>\n<p>\u5177\u4f53\u7684\u5e94\u7528\u5b9e\u4f8b\u8bf7\u53c2\u8003\u3001\u300c<a href=\"http:\/\/news.sciencenet.cn\/htmlnews\/2008\/10\/211752.html\">2008\u5e74\u8bfa\u6bd4\u5c14\u5316\u5b66\u5956<\/a>\u300d\u3002<\/p>\n<p>&nbsp;<\/p>\n<h4>\u95a2\u9023\u6587\u732e<\/h4>\n<ol>\n<li>\u00a0Shimomura, O. et al. <em>J. Cell. Comp. Physiol.<\/em> <strong>1962<\/strong>, <em>59,<\/em> 223. doi:<a href=\"http:\/\/dx.doi.org\/10.1002\/jcp.1030590302\">10.1002\/jcp.1030590302<\/a><\/li>\n<li>\u00a0(a) Tsien, R. Y.; Prasher, D. et al. <em>Science<\/em> <strong>1996<\/strong>, <em>273<\/em>, 1392. DOI: <a href=\"http:\/\/dx.doi.org\/10.1126\/science.273.5280.1392\">10.1126\/science.273.5280.1392<\/a> (b) Phillips, G. N. Jr. <em>Nat. Biotechnol.<\/em> <strong>1996<\/strong>, <em>14<\/em>, 1246. doi:<a href=\"http:\/\/dx.doi.org\/10.1038\/nbt1096-1246\">10.1038\/nbt1096-1246<\/a><\/li>\n<li>\u00a0Nienhaus, G. U.<em> Angew. Chem. Int. Ed.<\/em> <em>EarlyView.<\/em> <a href=\"http:\/\/dx.doi.org\/10.1002\/anie.200804998\">doi:10.1002\/anie.200804998<\/a><\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<h4>\u5173\u8054\u89c6\u9891<\/h4>\n<p><span class=\"embed-youtube\" style=\"text-align:center; display: block;\"><iframe loading=\"lazy\" class=\"youtube-player\" width=\"640\" height=\"360\" src=\"https:\/\/www.youtube.com\/embed\/Sfjt8D9WMGk?version=3&#038;rel=1&#038;showsearch=0&#038;showinfo=1&#038;iv_load_policy=1&#038;fs=1&#038;hl=en-US&#038;autohide=2&#038;wmode=transparent\" allowfullscreen=\"true\" style=\"border:0;\" sandbox=\"allow-scripts allow-same-origin allow-popups allow-presentation allow-popups-to-escape-sandbox\"><\/iframe><\/span><\/p>\n<p><b>\u5916\u90e8\u94fe\u63a5<\/b><\/p>\n<ul>\n<li><a href=\"http:\/\/en.wikipedia.org\/wiki\/Green_Fluorescent_Protein\">Green Fluorescent Protein \u2013 Wikipedia<\/a><\/li>\n<li><a href=\"http:\/\/www.scholarpedia.org\/article\/Fluorescent_proteins\">Fluorescent Proteins \u2013 Scholarpedia<\/a><\/li>\n<li><a href=\"http:\/\/wiredvision.jp\/news\/200810\/2008101623.html\">GFP\u5229\u7528\u306e\u907a\u4f1d\u5b50\u7d44\u307f\u63db\u3048\u751f\u7269\u3001\u753b\u50cf\u30ae\u30e3\u30e9\u30ea\u30fc<\/a>(WIRED VISION)<\/li>\n<li><a href=\"http:\/\/www.conncoll.edu\/ccacad\/zimmer\/GFP-ww\/GFP-1.htm\">Green Fluorescent Protein<\/a><\/li>\n<li><a href=\"http:\/\/nobelprize.org\/nobel_prizes\/chemistry\/laureates\/2008\/index.html\">Nobel Prize in Chemistry 2008<\/a><\/li>\n<li><a href=\"http:\/\/www.lifesci.ucsb.edu\/~biolum\/\">http:\/\/www.lifesci.ucsb.edu\/~biolum\/<\/a><\/li>\n<li><a href=\"http:\/\/www.rcsb.org\/pdb\/static.do?p=education_discussion\/molecule_of_the_month\/pdb42_1.html\">Green Fluorescent Protein (Protein Data Bank)<\/a><\/li>\n<li><a href=\"http:\/\/www.microscopyu.com\/articles\/livecellimaging\/fpintro.html\">Introduction to Fluorescent proteins<\/a><\/li>\n<li><a href=\"http:\/\/www.nature.com\/nmeth\/focus\/fluorescence\/classics\/proteins.html\">Milestone paper of Fluorescent Proteins<\/a> (Nature.com)<\/li>\n<\/ul>\n<p>\u672c\u6587\u7248\u6743\u5c5e\u4e8e<strong>\u00a0<a href=\"\/\/www.gsbet888.com\/\">Chem-Station\u5316\u5b66\u7a7a\u95f4<\/a>\uff0c<\/strong>\u00a0\u6b22\u8fce\u70b9\u51fb\u6309\u94ae\u5206\u4eab\uff0c\u672a\u7ecf\u8bb8\u53ef\uff0c\u8c22\u7edd\u8f6c\u8f7d\uff01<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"\u7eff\u8272\u8367\u5149\u86cb\u767d(Green Fluorescent Protein;GFP)\u3001\u662f\u4ece\u7ef4\u591a\u5229\u4e9a\u591a\u7ba1\u53d1\u5149\u6c34\u6bcd(Aequorea victoria)\u4e2d\u5206\u79bb\u51fa\u6765\u7684\u4e00\u79cd\u8367\u5149\u86cb\u767d\u3002 \u7528\u9014\u30fb\u5386\u53f2 \u8bfa\u5956\u5f97\u4e3b\u30fb\u4e0b\u6751\u8129\u7b49\u4eba\u57281962\u5e74\u4ece\u7ef4\u591a\u5229\u4e9a\u591a\u7ba1\u53d1\u5149\u6c34\u6bcd\u4e2d\u5206\u79bb\u30fb\u7cbe\u5236\u540e\u83b7\u5f97\u7684\u3002[1] (\u7167\u7247\uff1a\u5f15\u7528\u81eaWikipedia) \u5206\u5b50\u91cf\u7ea6\u4e3a27 kDa\u3001\u7531238\u4e2a\u6c28\u57fa\u9178\u7ec4\u6210\u3002 \u4e09\u7ef4\u7ed3\u6784\u57281996\u5e74\u901a\u8fc7X\u5c04\u7ebf\u5355\u6676\u884d\u5c04\u89e3\u6790\u5b8c\u6210[2]\u3002 \u5982\u6807\u9898\u56fe\u6240\u793a\uff0c11\u4e2a\u03b2\u5355\u4f4d\u662f\u4ee5\u87ba\u65cb\u72b6\u8fdb\u884c\u7ec4\u7ec7\u5316\u7684\uff0c\u5706\u67f1\u72b6\u7ed3\u6784\u4ee5\u5305\u56f4\u53d1\u5149\u56e2\uff08\u767d\u8272\u90e8\u5206\uff09\u7684\u5f62\u5f0f\u7ec4\u88c5\u3002\u8be5\u5706\u67f1\u7b52\u72b6\u7ed3\u6784\u53ef\u4ee5\u4fdd\u62a4\u5185\u90e8\u7684\u53d1\u5149\u56e2\uff0c\u63d0\u9ad8\u5176\u53d1\u5149\u6548\u7387\u4e0e\u5bff\u547d\u3002 \u5728\u7ef4\u591a\u5229\u4e9a\u591a\u7ba1\u53d1\u5149\u6c34\u6bcd\u7684\u4f53\u5185\u3001\u7eff\u8272\u8367\u5149\u86cb\u767d\u4e0e\u9499\u53cd\u5e94\u86cb\u767d\u6c34\u6bcd\u53d1\u5149\u86cb\u767d\uff08\u6c34\u6bcd\u53d1\u5149\u86cb\u767d\uff09\u7ed3\u5408\u53d1\u5149\u3002\u4e5f\u5c31\u662f\u8bf4\uff0c\u6c34\u6bcd\u53d1\u5149\u86cb\u767d\u9996\u5148\u5438\u6536\u80fd\u91cf\uff0cGFP\u63a5\u6536\u53d1\u5c04\u7684\u84dd\u8272\u8367\u5149\u5e76\u53d1\u51fa\u7eff\u5149\u3002 GFP\u7684\u53d1\u5149\u4f53\u5438\u6536\u8fd1\u7d2b\u5916\u5149\uff08397nm\uff09\u5e76\u53d1\u51fa\u7eff\u8272\u53ef\u89c1\u5149\uff08509nm\uff09\u3002\u53d1\u5149\u7ed3\u6784\u7531Ser65-Tyr66-Gly67\u4e09\u4e2a\u6c28\u57fa\u9178\u6b8b\u57fa\u901a\u8fc7\u73af\u5316\u30fb\u6c27\u5316\uff0c\u81ea\u53d1\u5f62\u6210\u3002\u8be5\u73af\u5316\u53cd\u5e94\u4e00\u822c\u6765\u8bf4\u975e\u5e38\u96be\u8fdb\u884c\uff0c\u7136\u800c\u5728GFP\u4e2d\u5f62\u6210\u4e86\u4e00\u79cd\u7279\u6b8a\u7684\u6784\u8c61\uff0c\u56e0\u6b64\u80fd\u591f\u5f88\u5bb9\u6613\u8fdb\u884c\u3002 \u540c\u6837\u5f0f\u8bfa\u8d1d\u5c14\u5956\u5f97\u4e3bChalfie\u30fbTsien\u7b49\u4eba\u53d1\u73b0\u4e86\u5229\u7528\u8be5\u53d1\u5149\u86cb\u767d\u8fdb\u884c\u8367\u5149\u6807\u8bb0\u7684\u65b9\u6cd5\uff0c\u56e0\u6b64\u76ee\u524d\u5316\u5b66\u751f\u7269\u5b66\u4e2d\u7684\u8367\u5149\u6807\u8bb0\u65b9\u6cd5\u51fa\u73b0\u4e86\u7206\u53d1\u6027\u7684\u53d1\u5c55\u3002 \u5177\u4f53\u7684\u5e94\u7528\u5b9e\u4f8b\u8bf7\u53c2\u8003\u3001\u300c2008\u5e74\u8bfa\u6bd4\u5c14\u5316\u5b66\u5956\u300d\u3002 &nbsp; \u95a2\u9023\u6587\u732e \u00a0Shimomura, O. et al. J. Cell. Comp. Physiol. 1962, 59, 223. doi:10.1002\/jcp.1030590302 \u00a0(a) Tsien, R. Y.; Prasher, D. et al. Science 1996, 273, 1392. DOI: 10.1126\/science.273.5280.1392 (b) Phillips, G. N. Jr. Nat. Biotechnol. 1996, 14, 1246. doi:10.1038\/nbt1096-1246 \u00a0Nienhaus, G. U. Angew. Chem. Int. Ed. 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