{"id":19579,"date":"2018-07-06T06:35:28","date_gmt":"2018-07-05T22:35:28","guid":{"rendered":"\/\/www.gsbet888.com\/?p=19579"},"modified":"2019-08-02T18:51:27","modified_gmt":"2019-08-02T10:51:27","slug":"%e4%b8%96%e7%95%8c%e8%91%97%e5%90%8d%e5%8c%96%e5%ad%a6%e5%ae%b6-%e5%ba%84%e5%b0%8f%e5%a8%81","status":"publish","type":"post","link":"\/\/www.gsbet888.com\/chemists\/2018\/07\/%e4%b8%96%e7%95%8c%e8%91%97%e5%90%8d%e5%8c%96%e5%ad%a6%e5%ae%b6-%e5%ba%84%e5%b0%8f%e5%a8%81.html","title":{"rendered":"\u4e16\u754c\u8457\u540d\u5316\u5b66\u5bb6\u2014\u2014\u5e84\u5c0f\u5a01"},"content":{"rendered":"
\u6295\u7a3f\u4f5c\u8005 \u6f02\u6cca<\/strong><\/p>\n \u5e84\u5c0f\u5a01\uff0c\u8457\u540d\u751f\u7269\u7269\u7406\u5b66\u5bb6\uff0c\u54c8\u4f5b\u5927\u5b66<\/a>\u5316\u5b66\u4e0e\u5316\u5b66\u751f\u7269\u3001\u7269\u7406\u5b66\u53cc\u8058\u6559\u6388\u3002\u5979\u62e5\u6709\u7f8e\u56fd\u56fd\u5bb6\u79d1\u5b66\u9662<\/a>\u9662\u58eb\u3001\u7f8e\u56fd\u827a\u672f\u4e0e\u79d1\u5b66\u5b66\u9662<\/a>\u9662\u58eb\u3001\u4e2d\u56fd\u79d1\u5b66\u9662<\/a>\u5916\u7c4d\u9662\u58eb\u7b49\u8bf8\u591a\u8363\u8a89\u3002\u5979\u53d1\u5c55\u5e76\u63a8\u5e7f\u4e86\u5355\u5206\u5b50\u751f\u7269\u63a2\u9488\u8fdb\u884c\u8d85\u9ad8\u5206\u8fa8\u751f\u7269\u6210\u50cf\u6280\u672f\uff08STORM\u6280\u672f\uff09\uff0c\u4f7f\u5f97\u5149\u5b66\u663e\u5fae\u955c\u5206\u8fa8\u80fd\u529b\u63a5\u8fd1\u7eb3\u7c73\u5c3a\u5ea6\u3002\u8be5\u6280\u672f\u6781\u5927\u7684\u63a8\u52a8\u4e86\u5bf9\u4f53\u5916\u53ca\u6d3b\u7ec6\u80de\u4e2d\u7684\u751f\u7269\u5206\u5b50\u53ca\u5176\u5206\u5b50\u7ec4\u88c5\u8fc7\u7a0b\u7684\u7814\u7a76\uff0c\u5979\u56e0\u6b64\u83b7\u5f97\u4e862003\u5e74\u7684\u9ea6\u514b\u963f\u745f\u5929\u624d\u5956\u3002\u5e84\u5c0f\u5a01\u6559\u6388\u662f\u8d85\u9ad8\u5206\u8fa8\u751f\u7269\u6210\u50cf\u53ca\u68c0\u6d4b\u9886\u57df\u7684\u9886\u519b\u4eba\u7269\uff0cSTORM\u6280\u672f\u7684\u53d1\u660e\u53ca\u5176\u5546\u4e1a\u5e94\u7528\u6781\u5927\u7684\u4fc3\u8fdb\u4e86\u4e16\u754c\u8303\u56f4\u5185\u7684\u751f\u7269\u533b\u5b66\u7814\u7a76\u3002[1-2]<\/sup><\/p>\n <\/p>\n 1987\u5e74\u4e2d\u56fd\u79d1\u6280\u5927\u5b66\u5c11\u5e74\u73ed\uff1b<\/p>\n 1991\u5e74\u7f8e\u56fd\u52a0\u5dde\u5927\u5b66\u4f2f\u514b\u5229\u5206\u6821<\/a>\u7269\u7406\u535a\u58eb\uff1b<\/p>\n 1996\u5e74\u65af\u5766\u798f\u5927\u5b66<\/a>\u535a\u58eb\u540e\uff0c\u5e08\u4ece\u6731\u68e3\u6587<\/a>\uff1b<\/p>\n 2001\u5e74\u54c8\u4f5b\u5927\u5b66\u5148\u540e\u62c5\u4efb\u52a9\u7406\u6559\u6388\u3001\u526f\u6559\u6388\uff1b<\/p>\n 2005\u5e74\u7f8e\u56fd\u970d\u534e\u5fb7\u00b7\u4f11\u65af\u533b\u5b66\u7814\u7a76\u6240<\/a>\u7814\u7a76\u5458\uff1b<\/p>\n 2006\u5e74\u54c8\u4f5b\u5927\u5b66\u5316\u5b66\u548c\u7269\u7406\u53cc\u5b66\u79d1\u6b63\u6559\u6388\uff0c<\/p>\n \u5e76\u5728\u54c8\u4f5b\u5927\u5b66\u5efa\u7acb\u4e86\u4ee5\u81ea\u5df1\u540d\u5b57\u547d\u540d\u7684\u5355\u5206\u5b50\u751f\u7269\u7269\u7406\u5b9e\u9a8c\u5ba4\uff1b<\/p>\n 2012\u5e74\u5f53\u9009\u4e3a\u7f8e\u56fd\u56fd\u5bb6\u79d1\u5b66\u9662\u9662\u58eb\uff1b<\/p>\n 2015\u5e74\u5f53\u9009\u4e3a\u4e2d\u56fd\u79d1\u5b66\u9662\u5916\u7c4d\u9662\u58eb<\/p>\n <\/p>\n 1991\u5e74\u00a0\u00a0 Bernice Teuteberg Memorial Scholarship, University of California<\/p>\n 1992\u5e74\u00a0\u00a0 George C. and Helen N. Pardee Scholarship, University of California<\/p>\n 1993\u5e74\u00a0\u00a0 Victor F. Lenzen Memorial Scholarship, University of California<\/p>\n 1997\u5e74\u00a0\u00a0 Chodorow Postdoctoral Fellowship, Stanford University<\/p>\n 2000\u5e74\u00a0\u00a0 \u7f8e\u56fd\u536b\u751f\u7814\u7a76\u9662\u56fd\u5bb6\u7814\u7a76\u4e2a\u4eba\u5956\uff08Individual National Research Service Award, National Institute of Health\uff09<\/p>\n 2002\u5e74\u00a0\u00a0 Young Investigator Award, Office of Naval Research<\/p>\n 2003\u5e74\u00a0\u00a0 \u7f8e\u56fd\u56fd\u5bb6\u81ea\u7136\u57fa\u91d1\u6210\u5c31\u5956\uff08CAREER award, National Science Foundation\uff09<\/p>\n 2003\u5e74\u00a0\u00a0 Beckman\u9752\u5e74\u79d1\u5b66\u5bb6\u5956\uff08Beckman Young Investigator Award\uff09\uff08\u5956\u91d124\u4e07\u7f8e\u91d1\uff09<\/p>\n 2003\u5e74\u00a0\u00a0 Searle\u5b66\u8005\u5956\uff08Searle Scholarship\uff09\uff08\u5956\u91d124\u4e07\u7f8e\u91d1\uff09<\/p>\n 2003\u5e74\u00a0\u00a0 \u9ea6\u514b\u963f\u745f\u5929\u624d\u5956\uff08MacArthur Fellowship\uff09\uff08\u5956\u91d150\u4e07\u7f8e\u91d1\uff09<\/p>\n 2003\u5e74\u00a0\u00a0 Packard Fellowship for Science and Engineering<\/p>\n 2003\u5e74\u00a0\u00a0 CAREER award, National Science Foundation<\/p>\n 2004\u5e74\u00a0\u00a0 Technology Review Worlds Top 100 Young Innovators Award<\/p>\n 2004\u5e74\u00a0\u00a0 Gunnar and Gunnel Kall\u00e9n Lecturer, Lund Univeristy, Sweden<\/p>\n 2004\u5e74\u00a0\u00a0 Jean-Franois Lef\u00e8vre Lecturer, Ecole Sup\u00e9rieure de Biotechnologie de Stansbourg, France<\/p>\n 2004\u5e74\u00a0\u00a0 Alfred P. Sloan Research Fellowship<\/p>\n 2005\u5e74\u00a0\u00a0 Camille Dreyfus Teacher-Scholar Award<\/p>\n 2005\u5e74\u00a0\u00a0 Howard Hughes Medical Institute Investigator<\/p>\n 2007\u5e74\u00a0\u00a0 \u7f8e\u56fd\u5316\u5b66\u5b66\u4f1a\u7eaf\u5316\u5b66\u5956\uff08Pure Chemistry Award\uff09<\/p>\n 2008\u5e74\u00a0\u00a0 Coblentz Award<\/p>\n 2008\u5e74\u00a0\u00a0 HHMI collaborative Innovation Award<\/p>\n 2010\u5e74\u00a0\u00a0 \u9a6c\u514b\u65af\u00b7\u5fb7\u5c14\u5e03\u5415\u514b\u751f\u7269\u7269\u7406\u5956\uff08Max Delbruck Prize in Biological Physics\uff09<\/p>\n 2011\u5e74\u00a0\u00a0 \u8d5b\u514b\u52d2\u56fd\u9645\u751f\u7269\u7269\u7406\u5b66\u5956\uff08Raymond & Beverly Sackler International Prize in Biophysics\uff09<\/p>\n 2014\u5e74\u00a0\u00a0 \u751f\u7269\u79d1\u6280\u9886\u57df\u4e16\u754c\u6280\u672f\u5956\uff08World Technology Award for Biotechnology\uff09<\/p>\n 2015\u5e74\u00a0\u00a0 \u5bbe\u5915\u6cd5\u5c3c\u4e9a\u5927\u5b66\u7eb3\u7c73\/\u751f\u7269\u754c\u9762\u7814\u7a76\u4e2d\u5fc3\u4f18\u79c0\u7814\u7a76\u5956\uff08Research Excellence Award\uff09<\/p>\n 2015\u5e74\u00a0\u00a0 \u82f1\u56fd\u738b\u5ba4\u663e\u5fae\u955c\u5b66\u4f1a\u76ae\u5c14\u65af\u5956\uff08Pearse Prize, Royal Microscopical Society\uff09<\/p>\n 2015\u5e74\u00a0\u00a0 \u7f8e\u56fd\u79d1\u5b66\u9662\u5206\u5b50\u751f\u7269\u5b66\u5956\uff08National Academy of Sciences Award inMolecular Biology\uff09<\/p>\n 2016\u5e74\u00a0\u00a0 \u65af\u5fb7\u54e5\u5c14\u6469\u5927\u5b66\u8363\u8a89\u535a\u58eb\uff08Doctor of Philosophy Honoris Causa\uff09<\/p>\n 2017\u5e74\u00a0\u00a0 \u745e\u5178\u5361\u7f57\u6797\u65af\u5361\u5b66\u9662\u4f26\u7eb3\u7279\u00b7\u5c3c\u5c14\u68ee\u5956\uff08Lennart Nilsson Award\uff09<\/p>\n 2017\u5e74\u00a0\u00a0 \u5339\u5179\u5821\u5206\u6790\u5316\u5b66\u5956\uff08Pittsburgh Analytical Chemistry Award\uff09<\/p>\n <\/p>\n 1\u3001\u8d85\u5206\u8fa8\u8367\u5149\u663e\u5fae\u955c\uff08STORM\u6280\u672f\uff09<\/p>\n \u7531\u4e8e\u884d\u5c04\u73b0\u8c61\u7684\u5b58\u5728\uff0c\u5229\u7528\u5149\u5b66\u663e\u5fae\u955c\u89c2\u5bdf\u5c3a\u5bf8\u5f88\u5c0f\u7684\u7269\u8d28\u5b58\u5728\u4e00\u4e2a\u884d\u5c04\u6781\u9650\uff0c\u8fd9\u88ab\u79f0\u4e3a\u963f\u8d1d\u6781\u9650\uff0c\u666e\u901a\u7684\u5149\u5b66\u663e\u5fae\u955c\u7684\u884d\u5c04\u6781\u9650\u4e3a200nm\uff0c\u8fd9\u4e0e\u7ec6\u80de\u7684\u5c3a\u5bf8\u76f8\u4f3c\uff0c\u4f46\u662f\u65e0\u6cd5\u5b9e\u73b0\u5bf9\u7ec6\u80de\u5185\u66f4\u7cbe\u7ec6\u7ed3\u6784\u7684\u89c2\u6d4b\u3002<\/p>\n \u4e3a\u4e86\u5b9e\u73b0\u5bf9\u7ec6\u80de\u5185\u66f4\u7cbe\u7ec6\u7ed3\u6784\u7684\u8d85\u9ad8\u5206\u8fa8\u68c0\u6d4b\u30022006\u5e74\u5e95\uff0c\u5e84\u5c0f\u5a01\u8bfe\u9898\u7ec4\u53d1\u660e\u4e86\u4e00\u79cd\u53ef\u4ee5\u7528\u6765\u7814\u7a76\u7ec6\u80de\u5185\u6e90\u86cb\u767d\u7684\u8d85\u5206\u8fa8\u7387\u5b9a\u4f4d\u7684\u65b9\u6cd5\uff08STORM\u6280\u672f\uff09\u3002\u4ed6\u4eec\u53d1\u73b0\u4e0d\u540c\u7684\u6ce2\u957f\u53ef\u4ee5\u63a7\u5236\u5316\u5b66\u8367\u5149\u5206\u5b50Cy5 \u5728\u8367\u5149\u6fc0\u53d1\u6001\u548c\u6697\u6001\u4e4b\u95f4\u5207\u6362\uff0c\u4f8b\u5982\u7ea2\u8272561nm\u7684\u6fc0\u5149\u53ef\u4ee5\u6fc0\u6d3bCy5\u53d1\u5c04\u8367\u5149\uff0c\u540c\u65f6\u957f\u65f6\u95f4\u7167\u5c04\u53ef\u4ee5\u5c06Cy5 \u5206\u5b50\u8f6c\u6362\u6210\u6697\u6001\u4e0d\u53d1\u5149\uff1b\u4e4b\u540e\u7528\u7eff\u8272\u7684488nm\u6fc0\u5149\u7167\u5c04Cy5 \u5206\u5b50\u65f6\uff0c\u53ef\u4ee5\u5c06\u5176\u4ece\u6697\u6001\u8f6c\u6362\u6210\u8367\u5149\u6001\uff0c\u800c\u6b64\u8fc7\u7a0b\u7684\u957f\u77ed\u4f9d\u8d56\u4e8e\u7b2c\u4e8c\u4e2a\u8367\u5149\u5206\u5b50Cy3 \u4e0eCy5 \u4e4b\u95f4\u7684\u8ddd\u79bb\u3002\u56e0\u6b64\uff0c\u5f53Cy3 \u548cCy5 \u4ea4\u8054\u6210\u5206\u5b50\u5bf9\u65f6\uff0c\u5177\u5907\u4e86\u7279\u5b9a\u7684\u6fc0\u53d1\u5149\u8f6c\u6362\u8367\u5149\u5206\u5b50\u53d1\u5c04\u6ce2\u957f\u7684\u7279\u6027\u3002\u5c06Cy3 \u548cCy5 \u5206\u5b50\u5bf9\u80f6\u8054\u5230\u7279\u5f02\u7684\u86cb\u767d\u8d28\u6297\u4f53\u4e0a\uff0c\u5c31\u53ef\u4ee5\u7528\u6297\u4f53\u6765\u6807\u8bb0\u7ec6\u80de\u7684\u5185\u6e90\u86cb\u767d\u8d28\u3002\u5e94\u7528\u7279\u5b9a\u6ce2\u957f\u7684\u6fc0\u5149\u6765\u6fc0\u6d3b\u63a2\u9488\uff0c\u7136\u540e\u5e94\u7528\u53e6\u4e00\u4e2a\u6ce2\u957f\u6fc0\u5149\u6765\u89c2\u5bdf\u3001\u7cbe\u786e\u5b9a\u4f4d\u4ee5\u53ca\u6f02\u767d\u8367\u5149\u5206\u5b50\uff0c\u6b64\u8fc7\u7a0b\u5faa\u73af\u4e0a\u767e\u6b21\u540e\u5c31\u53ef\u4ee5\u5f97\u5230\u6700\u540e\u7684\u5185\u6e90\u86cb\u767d\u8d28\u7684\u9ad8\u5206\u8fa8\u7387\u5f71\u50cf\u3002\u5229\u7528\u8be5\u6280\u672f\uff0c\u53ef\u4ee5\u5b9e\u73b0\u5bf9\u7ec6\u80de\u7cbe\u7ec6\u7ed3\u6784\u7684\u8d85\u9ad8\u5206\u8fa8\u68c0\u6d4b\uff0c\u8be5\u65b9\u6cd5\u548cSTED\u6280\u672f\u4ee5\u53caPLAM\u6280\u672f\u4e00\u8d77\uff0c\u5c06\u5149\u5b66\u663e\u5fae\u6280\u672f\u7684\u884d\u5c04\u6781\u9650\u53c8\u7f29\u5c0f\u4e86\u51e0\u4e2a\u6570\u91cf\u7ea7\uff0c\u6781\u5927\u7684\u4fc3\u8fdb\u4e86\u751f\u7269\u533b\u5b66\u7684\u7814\u7a76\u3002[3]<\/sup><\/p>\n <\/p>\n STORM\u6280\u672f\u539f\u7406\u53ca\u6210\u50cf\u7ed3\u679c<\/p>\n <\/p>\n 2\u3001\u5355\u5206\u5b50\u751f\u7269\u5b66\u68c0\u6d4b<\/p>\n \u5e84\u5c0f\u5a01\u8bfe\u9898\u7ec4\u5229\u7528\u5355\u5206\u5b50\u8367\u5149\u6210\u50cf\u6280\u672f\u548c\u5176\u4ed6\u5149\u8c31\u6280\u672f\u961f\u5728\u4f53\u5916\u5bf9\u590d\u6742\u7684\u751f\u7269\u5206\u5b50\u7cfb\u7edf\u8fdb\u884c\u4e86\u4e00\u7cfb\u5217\u7814\u7a76\u3002\u5979\u4eec\u5229\u7528\u9ad8\u5ea6\u654f\u611f\u7684\u8ddd\u79bb\u4f9d\u8d56\u8367\u5149\u5171\u632f\u80fd\u91cf\u8f6c\u79fb(FRET)\u5bf9\u5355\u5206\u5b50\u5c3a\u5ea6\u4e0a\u7684\u7ed3\u6784\u53d8\u5316\u8fdb\u884c\u4e86\u68c0\u6d4b\uff0c\u540c\u65f6\u4e5f\u5bf9\u590d\u6742\u751f\u7269\u4f53\u7cfb\u4e2d\u7684\u52a8\u529b\u5b66\u673a\u5236\u8fdb\u884c\u4e86\u7814\u7a76\u3002\u5229\u7528\u5355\u5206\u5b50\u6210\u50cf\u6280\u672f\uff0c\u5979\u4eec\u4e5f\u5bf9\u7ec6\u80de\u4e2d\u86cb\u767d\u8d28\u4e0e\u6838\u9178\u7684\u76f8\u4e92\u4f5c\u7528\u8fdb\u884c\u4e86\u4e00\u4e9b\u7814\u7a76\uff0c\u5982\u7814\u7a76DNA\u590d\u5236\uff0c\u86cb\u767d\u8d28\u7ffb\u8bd1\u7b49\u91cd\u8981\u7684\u751f\u7406\u8fc7\u7a0b\uff0c\u5e76\u5b9e\u73b0\u4e86\u5bf9\u5b83\u4eec\u7ec4\u88c5\u8fc7\u7a0b\u7684\u6210\u50cf\u5206\u6790\u3002\u6b64\u5916\uff0c\u4e00\u4e9b\u67d3\u8272\u4f53\u548c\u9176\u7684\u7ed3\u6784\u4ee5\u53ca\u5b83\u4eec\u7684\u76f8\u4e92\u4f5c\u7528\u673a\u5236\u4e5f\u5f97\u5230\u4e86\u68c0\u6d4b\u4e0e\u5206\u6790\u3002[3]<\/sup><\/p>\n 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\u6240\u83b7\u8363\u8a89<\/strong><\/h4>\n
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\u4e3b\u8981\u7814\u7a76\u5de5\u4f5c<\/strong><\/h4>\n
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\u4ee3\u8868\u6027\u8bba\u6587<\/strong><\/h4>\n[1]Boettiger A N, Bintu B, Moffitt J R, et al. Super-resolution imaging reveals distinct chromatin folding for different epigenetic states[J]. Nature, 2016, 529(7586): 418.<\/a><\/p>\n[2]Sigal Y M, Speer C M, Babcock H P, et al. Mapping synaptic input fields of neurons with super-resolution imaging[J]. Cell, 2015, 163(2): 493-505.<\/a><\/p>\n[3] Hwang W L, Deindl S, Harada B T, et al. Histone H4 tail mediates allosteric regulation of nucleosome remodelling by linker DNA[J]. Nature, 2014, 512(7513): 213.<\/a><\/p>\n[4] Xu K, Zhong G, Zhuang X. Actin, spectrin, and associated proteins form a periodic cytoskeletal structure in axons[J]. Science, 2013, 339(6118): 452-456.<\/a><\/p>\n[5] Berk V, Fong J C N, Dempsey G T, et al. Molecular architecture and assembly principles of Vibrio cholerae biofilms[J]. Science, 2012, 337(6091): 236-239.<\/a><\/p>\n
<\/h4>\n
\u5176\u4ed6\u53c2\u8003\u6587\u732e<\/strong><\/h4>\n[1]https:\/\/www.aps.org\/programs\/honors\/prizes\/prizerecipient.cfm?last_nm=Zhuang&first_nm=Xiaowei&year=2010<\/a><\/p>\n[2]https:\/\/english.tau.ac.il\/sackler_prize_in_biophysics_past_laureates<\/a><\/p>\n[3]http:\/\/zhuang.harvard.edu<\/a><\/p>\n