{"id":29072,"date":"2019-10-30T18:27:26","date_gmt":"2019-10-30T10:27:26","guid":{"rendered":"\/\/www.gsbet888.com\/?p=29072"},"modified":"2019-10-30T18:27:26","modified_gmt":"2019-10-30T10:27:26","slug":"leroy-e-hood","status":"publish","type":"post","link":"\/\/www.gsbet888.com\/chemists\/2019\/10\/leroy-e-hood.html","title":{"rendered":"Leroy E. Hood"},"content":{"rendered":"<p>Leroy E. Hood\uff1b 1938\u5e7410\u670810\u65e5-\u3001\u662f\u7f8e\u56fd\u7684\u751f\u7269\u5b66\u5bb6\u3002\u534e\u76db\u987f\u5927\u5b66\u6559\u6388\u3002<strong>DNA \/\u86cb\u767d\u8d28\u81ea\u52a8\u6d4b\u5e8f\u4eea\uff0c\u81ea\u52a8\u5408\u6210\u4eea<\/strong>\u7b49\u7684\u5f00\u53d1\u8005\u3002\u4ed6\u5f00\u53d1\u7684\u8fd9\u4e9b\u8bbe\u5907\u4e3a\u57fa\u56e0\u7ec4\u5b66\u548c\u86cb\u767d\u8d28\u7ec4\u5b66\u7684\u53d1\u5c55\u4ee5\u53ca\u751f\u7269\u5b66\uff0c\u5316\u5b66\u548c\u533b\u5b66\u9886\u57df\u7684\u7814\u7a76\u505a\u51fa\u4e86\u5de8\u5927\u8d21\u732e\u3002<\/p>\n<h4>\u7ecf\u5386<\/h4>\n<p>19XX \u52a0\u5dde\u7406\u5de5\u5b66\u9662 \u6bd5\u4e1a<br \/>\n1964 \u7ea6\u7ff0\u00b7\u970d\u666e\u91d1\u65af\u5927\u5b66 \u533b\u5b66\u535a\u58eb\u5b66\u4f4d<br \/>\n1968 \u52a0\u5dde\u7406\u5de5\u5b66\u9662 \u535a\u58eb\u540e\uff08\u6307\u5bfc\u6559\u6388\uff1aWilliam J. Dreyer\uff09<br \/>\n1970 \u52a0\u5dde\u7406\u5de5\u5b66\u9662 \u52a9\u7406\u6559\u6388<br \/>\n1973 \u52a0\u5dde\u7406\u5de5\u5b66\u9662 \u526f\u6559\u6388<br \/>\n1975 \u52a0\u5dde\u7406\u5de5\u5b66\u9662 \u6559\u6388<br \/>\n1980-1989 \u52a0\u5dde\u7406\u5de5\u5b66\u9662 \u751f\u7269\u5b66\u79d1 \u5b66\u79d1\u957f<br \/>\n1992 \u534e\u76db\u987f\u5927\u5b66 \u6559\u6388<br \/>\n2000 Institute for System Biology \u6240\u957f<\/p>\n<h4>\u83b7\u5956\u7ecf\u5386<\/h4>\n<p>2002 \u4eac\u90fd\u8d4f<br \/>\n2003 Lemelson\u2013MIT Prize<br \/>\n2006 Heinz Award in Technology, the Economy &amp; Employment<br \/>\n2009 Pittcon Heritage Award<br \/>\n2010 Kistler Prize<br \/>\n2011 National Medal of Science<br \/>\n2014 IEEE Medal for Innovations in Healthcare Technology<br \/>\n2019 \u79d1\u777f\u552f\u5b89\u5f15\u6587\u6842\u51a0\u5956<\/p>\n<h4>\u7814\u7a76\u4e1a\u7ee9<\/h4>\n<p><strong>\u9ad8\u7075\u654f\u5ea6\u7684\u86cb\u767d\u8d28\u6d4b\u5e8f\u4eea\u7684\u5f00\u53d1<\/strong><\/p>\n<p>\u5f53Hood\u6559\u6388\u5f00\u59cb\u5728\u52a0\u5dde\u7406\u5de5\u5b66\u9662\u5b66\u4e60\u65f6\uff0c\u4ed6\u5c31\u8ba1\u5212\u5c06\u81ea\u5df1\u7684\u7814\u7a76\u751f\u6daf\u6295\u5165\u751f\u7269\u5b66\u548c\u4eea\u5668\u79d1\u6280\u9886\u57df\u3002\u81ea\u4ece\u7814\u7a76\u86cb\u767d\u8d28\u5316\u5b66\u4ee5\u6765\uff0c\u4ed6\u9996\u5148\u5bf9\u5229\u7528\u57c3\u5fb7\u66fc\u964d\u89e3\u6cd5\u6539\u826f\u86cb\u767d\u8d28\u6d4b\u5e8f\u4eea\u4ea7\u751f\u4e86\u5174\u8da3\u3002\u5c31\u5f53\u65f6\u7684\u6280\u672f\u800c\u8a00\uff0c\u6bcf\u6b21\u5206\u6790\u9700\u8981\u5927\u91cf\u6837\u54c1\uff08\u6beb\u514b\u7ea7\uff09\u3001Hood\u6559\u6388\u8fdb\u884c\u4e86\u5982\u4e0b\u6539\u826f<u>\uff08<\/u><u>1<\/u><u>\uff09<\/u><u>\u901a\u8fc7\u5728\u6c14\u76f8\u800c\u4e0d\u662f\u6db2\u76f8\u4e2d\u8fdb\u884c\u57c3\u5fb7\u66fc\u964d\u89e3\u6765\u51cf\u5c11\u6837\u54c1\u635f\u5931\u3001\uff08<\/u><u>2<\/u><u>\uff09\u5f00\u53d1\u4e86\u53ef\u4ee5\u627f\u53d7\u8150\u8680\u6027\u57c3\u5fb7\u66fc\u8bd5\u5242\u7684\u9600\u95e8<\/u>\u3001\u4ece\u800c\u5f00\u53d1\u4e86\u9ad8\u7075\u654f\u7684\u6c14\u76f8\u6db2\u76f8\u86cb\u767d\u6d4b\u5e8f\u4eea\uff0c\u53ef\u5c06\u6240\u9700\u6837\u54c1\u91cf\u51cf\u5c11\u81f3\u5fae\u514b\u7ea7\uff081982\u5e74\uff09\u3002<\/p>\n<p><strong>DNA\u30fb\u80bd\u81ea\u52a8\u5408\u6210\u4eea\u7684\u5f00\u53d1<\/strong><\/p>\n<p>\u5927\u7ea6\u57281970\u5e74\u4ee3\u672b\uff0c\u79d1\u7f57\u62c9\u591a\u5927\u5b66\u7684Marvin Caruthers\u6559\u6388\u5f00\u53d1\u4e86\u4e00\u79cd\u7528\u4e8eDNA\u7684\u5316\u5b66\u5408\u6210\u65b9\u6cd5\uff08<strong>\u4e9a\u78f7\u9170\u80fa\u6cd5<\/strong>\uff09\u3002\u3002\u5bf9\u5f00\u53d1\u81ea\u52a8\u5316DNA\u5408\u6210\u4eea\u611f\u5174\u8da3\u7684Hood\u6559\u6388\u8bf7Caruthers\u6559\u6388\u8bb2\u6388\u4e9a\u78f7\u9170\u80fa\u6cd5\uff0c\u5e76\u901a\u8fc7\u5c06Caruthers\u6559\u6388\u5f00\u53d1\u7684\u53cd\u5e94\u8fdb\u884c\u81ea\u52a8\u5316\u6765\u5f00\u53d1\u81ea\u52a8\u5316DNA\u5408\u6210\u4eea\uff081983\uff09\u3002\u6b64\u5916\uff0c\u4ed6\u5229\u7528\u5728\u86cb\u767d\u8d28\u6d4b\u5e8f\u4eea\u548cDNA\u5408\u6210\u4eea\u65b9\u9762\u7684\u5f00\u53d1\u7ecf\u9a8c\u5f00\u53d1\u4e86\u80bd\u5408\u6210\u4eea\uff081984\u5e74\uff09\u3002<\/p>\n<p><strong>DNA\u81ea\u52a8\u6d4b\u5e8f\u4eea\u7684\u5f00\u53d1<\/strong><\/p>\n<p>1975\u5e74\u3001Walter Gilbert\u6559\u6388\u30fbFrederick Sanger\u6559\u6388\u3001\u5206\u522b\u5f00\u53d1\u4e86\u4f7f\u7528\u5316\u5b66\u964d\u89e3\u00b7\u9176\u7684DNA\u6d4b\u5e8f\u65b9\u6cd5\uff081980\u5e74\u3001\u8bfa\u8d1d\u5c14\u5316\u5b66\u5956\uff09\u3002Hood\u7b49\u4eba\u5bf9\u8fd9\u79cd\u65b9\u6cd5\u7684\u81ea\u52a8\u5316\u5f88\u611f\u5174\u8da3\uff0c\u5e76\u6839\u636eSanger\u6559\u6388\u62a5\u9053\u7684\u65b9\u6cd5\uff08\u53cc\u8131\u6c27\u6cd5\/ Sanger\u6cd5\uff09\u8fdb\u884c\u4e86DNA\u6d4b\u5e8f\u4eea\u7684\u5f00\u53d1\u3002<\/p>\n<div id=\"attachment_68070\" class=\"wp-caption aligncenter\">\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-medium wp-image-29074\" src=\"https:\/\/assets.www.gsbet888.com\/uploads\/2019\/10\/f216dabf7344dfc6c4fcecf69452e3ac-300x115.png\" alt=\"\" width=\"300\" height=\"115\" srcset=\"https:\/\/assets.www.gsbet888.com\/uploads\/2019\/10\/f216dabf7344dfc6c4fcecf69452e3ac-300x115.png 300w, https:\/\/assets.www.gsbet888.com\/uploads\/2019\/10\/f216dabf7344dfc6c4fcecf69452e3ac.png 351w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/p>\n<p class=\"wp-caption-text\">\u56fe1. \u8367\u5149\u6807\u8bb0\u7684\u53cc\u8131\u6c27\u6838\u82f7\u9178\u4e09\u78f7\u9178\uff08Dye-ddNTP\uff09\u3002<\/p>\n<\/div>\n<p>\u5728Hood\u6559\u6388\u7684\u65b9\u6cd5\u4e2d\uff0c\u5c06<strong>\u5c11\u91cf\u8367\u5149\u6807\u8bb0\u7684\u53cc\u8131\u6c27\u6838\u82f7\u9178\u4e09\u78f7\u9178\uff08ddNTP\uff09<\/strong>\u6df7\u5408\u5728DNA\u6269\u589e\u6eb6\u6db2\u4e2d\uff08\u56fe1\uff09\u3002\u7531\u4e8eddNTP\u57283&#8217;\u4f4d\u6ca1\u6709\u7f9f\u57fa\uff0c\u56e0\u6b64\u5f53\u901a\u8fc7DNA\u805a\u5408\u9176\u63ba\u5165\u65f6\uff0cDNA\u5ef6\u4f38\u5728\u90a3\u91cc\u505c\u6b62\uff08\u56fe2\uff09\u3002<\/p>\n<div id=\"attachment_68071\" class=\"wp-caption aligncenter\">\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-29075 size-large\" src=\"https:\/\/assets.www.gsbet888.com\/uploads\/2019\/10\/7dd4c3f74f006b34bb1d70d7adebd54e-680x494.png\" alt=\"\" width=\"680\" height=\"494\" srcset=\"https:\/\/assets.www.gsbet888.com\/uploads\/2019\/10\/7dd4c3f74f006b34bb1d70d7adebd54e-680x494.png 680w, https:\/\/assets.www.gsbet888.com\/uploads\/2019\/10\/7dd4c3f74f006b34bb1d70d7adebd54e-300x218.png 300w, https:\/\/assets.www.gsbet888.com\/uploads\/2019\/10\/7dd4c3f74f006b34bb1d70d7adebd54e-768x558.png 768w, https:\/\/assets.www.gsbet888.com\/uploads\/2019\/10\/7dd4c3f74f006b34bb1d70d7adebd54e.png 1220w\" sizes=\"(max-width: 680px) 100vw, 680px\" \/><\/p>\n<p class=\"wp-caption-text\">\u56fe2. Hood\u7b49\u4eba\u7684DNA\u6d4b\u5e8f\u4eea\u673a\u5236\u3002<\/p>\n<\/div>\n<p>\u4ece\u8fd9\u79cd\u53cd\u5e94\u6eb6\u6db2\u83b7\u5f97\u7684\u4ea7\u7269\u662f\u5728\u5404\u4e2a\u4f4d\u7f6e\u7247\u6bb5\u5316\u7684DNA\u4ea7\u7269\u3002\u901a\u8fc7\u6bdb\u7ec6\u7ba1\u7535\u6cf3\u6839\u636e\u957f\u5ea6\u5c06\u5176\u5206\u79bb\uff0c\u5e76\u8bfb\u53d6\u4e0e\u6bcf\u4e2a\u78b1\u57fa\u76f8\u5bf9\u5e94\u7684\u8367\u5149\u4fe1\u53f7\u4ee5\u786e\u5b9a\u6a21\u677fDNA\u7684\u5e8f\u5217\u3002Sanger\u6559\u6388\u7b49\u4eba\u5f00\u53d1\u4e86ddNTP\u6cd5\uff08\u8131\u6c27\u6cd5\/ Sanger\u6cd5\uff09\uff0c\u4f46\u4ed6\u4eec\u7684\u65b9\u6cd5\u4f7f\u7528\u4e86\u653e\u5c04\u6027\u540c\u4f4d\u7d20\u6807\u8bb0\uff0c\u5e76\u5728\u6bcf\u6b21\u53cd\u5e94\u4e2d\u6dfb\u52a0\u4e86\u56db\u4e2addNTP\u4e2d\u7684\u4e00\u4e2a\uff0c\u4e5f\u5c31\u662f\u8bf4\uff0c\u6709\u5fc5\u8981\u5206\u522b\u8fdb\u884c\u56db\u4e2a\u53cd\u5e94\u3002\u4f5c\u4e3aSanger\u65b9\u6cd5\u7684\u6539\u8fdb\u7248\u672c\uff0cHood\u7b49\u4eba\u65b9\u6cd5\u662f\u7528\u56db\u79cd\u4e0d\u540c\u7684\u8367\u5149\u67d3\u6599\u6807\u8bb0ddNTP\uff0c\u7136\u540e\u540c\u65f6\u6dfb\u52a0\u5b83\u4eec\uff0c\u4ece\u800c\u4e00\u6b21\u6027\u53ef\u4ee5\u5728\u5355\u4e2a\u6bdb\u7ec6\u7ba1\u4e2d\u5206\u79bb\u548c\u5206\u6790\u4ea7\u7269\u3002\u00a0\u6b64\u5916\uff0c\u8fd8\u514b\u670d\u4e86\u81ea\u52a8\u5316\u65b9\u9762\u7684\u5404\u79cd\u56f0\u96be\uff0c\u5e76\u4e8e1986\u5e74\u6210\u529f\u5f00\u53d1\u4e86\u81ea\u52a8DNA\u6d4b\u5e8f\u4eea\u3002<\/p>\n<p>\u52a0\u5dde\u7406\u5de5\u5b66\u9662\u7684Mike Hunkapiller\u535a\u58eb\u548cLloyd Smith\u535a\u58eb\uff08\u73b0\u4e3a\u5a01\u65af\u5eb7\u661f\u5927\u5b66\u6559\u6388\uff09\u90fd\u53c2\u4e0e\u4e86\u8be5\u7cfb\u5217\u8bbe\u5907\u7684\u5f00\u53d1\u3002\u6b64\u5916\uff0c\u8fd9\u4e9b\u8bbe\u5907\u901a\u8fc7\u4e00\u5bb6\u540d\u4e3aApplied Biosystems\u7684\u516c\u53f8\u8fdb\u884c\u4e86\u5546\u4e1a\u5316\uff0c\u5e76\u5e7f\u4e3a\u666e\u53ca\u3002<\/p>\n<p>&nbsp;<\/p>\n<h4>\u5176\u4ed6<\/h4>\n<p>\u5f53Hood\u6559\u6388\u4e0eCaruthers\u6559\u6388\u8c08\u8bba\u81ea\u52a8DNA\u5408\u6210\u4eea\u65f6\uff0cCaruthers\u6559\u6388\u60f3\u77e5\u9053\u4e3a\u4ec0\u4e48Hood\u6559\u6388\u4f1a\u8d39\u5fc3\u5730\u4f7f\u6838\u9178\u5408\u6210\u81ea\u52a8\u5316\u3002\u56e0\u4e3a\u5f53\u65f6\uff0c\u57fa\u56e0\u7ec4\u5b66\u548c\u86cb\u767d\u8d28\u7ec4\u5b66\u9886\u57df\u8fd8\u6ca1\u6709\u5f97\u5230\u53d1\u5c55\uff0c\u56e0\u6b64\uff0c\u4eba\u4eec\u5bf9\u6838\u9178\u5408\u6210\u4eea\u7684\u9700\u6c42\u9884\u8ba1\u4e0d\u4f1a\u589e\u957f\u3002<\/p>\n<p>&nbsp;<\/p>\n<h4>\u76f8\u5173\u6587\u732e<\/h4>\n<ol>\n<li>Hewick, R. M.; Hunkapiller, M. W.; Hood, L. E.; Dreyer, W. J. <em>J. Biol. Chem<\/em>. <strong>1981<\/strong>, <em>256<\/em>, 7990. <a href=\"http:\/\/www.jbc.org\/content\/256\/15\/7990.long\">Link<\/a><\/li>\n<li>Hunkapiller, M. W.; Hood, L. E. <em>Science<\/em> <strong>1980<\/strong>, <em>207<\/em>, 523. DOI: <a href=\"https:\/\/science.sciencemag.org\/content\/207\/4430\/523\/tab-pdf\">10.1126\/science.7352258<\/a><\/li>\n<li>Smith, L. M.; Sanders, J. Z.; Kaiser, R. J.; Hughes, P.; Dodd, C. Connell, C. R.; Heiner, C.; Kent, S. B. <em>Nature<\/em> 1986, 321, 674. <a href=\"https:\/\/www.nature.com\/articles\/321674a0\">Link<\/a><\/li>\n<li>Hood, L. <em>Annu. Rev. Anal. Chem.<\/em> <strong>2008<\/strong>, <em>1<\/em>, 1. DOI:\u00a0<a href=\"https:\/\/www.annualreviews.org\/doi\/10.1146\/annurev.anchem.1.031207.113113\">10.1146\/annurev.anchem.1.031207.113113<\/a><\/li>\n<\/ol>\n<h4>\u76f8\u5173\u94fe\u63a5<\/h4>\n<ul>\n<li><a href=\"https:\/\/zh.wikipedia.org\/wiki\/DNA\u6e2c\u5e8f\">DNA\u6d4b\u5e8f<\/a>: wikipedia<\/li>\n<li><a href=\"https:\/\/www.immunology.washington.edu\/faculty\/affiliate-faculty\/hood\/\">Leroy Hood<\/a>\uff1a\u534e\u76db\u987f\u5927\u5b66\u4e3b\u9875<\/li>\n<li><a href=\"https:\/\/en.wikipedia.org\/wiki\/Applied_Biosystems\">Applied Biosystems<\/a>\uff1awikipedia<\/li>\n<\/ul>\n<p><strong>\u672c\u6587\u7248\u6743\u5c5e\u4e8e\u00a0<a href=\"\/\/www.gsbet888.com\/\">Chem-Station\u5316\u5b66\u7a7a\u95f4<\/a>\uff0c \u6b22\u8fce\u70b9\u51fb\u6309\u94ae\u5206\u4eab\uff0c\u672a\u7ecf\u8bb8\u53ef\uff0c\u8c22\u7edd\u8f6c\u8f7d\uff01<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"Leroy E. Hood\uff1b 1938\u5e7410\u670810\u65e5-\u3001\u662f\u7f8e\u56fd\u7684\u751f\u7269\u5b66\u5bb6\u3002\u534e\u76db\u987f\u5927\u5b66\u6559\u6388\u3002DNA \/\u86cb\u767d\u8d28\u81ea\u52a8\u6d4b\u5e8f\u4eea\uff0c\u81ea\u52a8\u5408\u6210\u4eea\u7b49\u7684\u5f00\u53d1\u8005\u3002\u4ed6\u5f00\u53d1\u7684\u8fd9\u4e9b\u8bbe\u5907\u4e3a\u57fa\u56e0\u7ec4\u5b66\u548c\u86cb\u767d\u8d28\u7ec4\u5b66\u7684\u53d1\u5c55\u4ee5\u53ca\u751f\u7269\u5b66\uff0c\u5316\u5b66\u548c\u533b\u5b66\u9886\u57df\u7684\u7814\u7a76\u505a\u51fa\u4e86\u5de8\u5927\u8d21\u732e\u3002 \u7ecf\u5386 19XX \u52a0\u5dde\u7406\u5de5\u5b66\u9662 \u6bd5\u4e1a 1964 \u7ea6\u7ff0\u00b7\u970d\u666e\u91d1\u65af\u5927\u5b66 \u533b\u5b66\u535a\u58eb\u5b66\u4f4d 1968 \u52a0\u5dde\u7406\u5de5\u5b66\u9662 \u535a\u58eb\u540e\uff08\u6307\u5bfc\u6559\u6388\uff1aWilliam J. Dreyer\uff09 1970 \u52a0\u5dde\u7406\u5de5\u5b66\u9662 \u52a9\u7406\u6559\u6388 1973 \u52a0\u5dde\u7406\u5de5\u5b66\u9662 \u526f\u6559\u6388 1975 \u52a0\u5dde\u7406\u5de5\u5b66\u9662 \u6559\u6388 1980-1989 \u52a0\u5dde\u7406\u5de5\u5b66\u9662 \u751f\u7269\u5b66\u79d1 \u5b66\u79d1\u957f 1992 \u534e\u76db\u987f\u5927\u5b66 \u6559\u6388 2000 Institute for System Biology \u6240\u957f \u83b7\u5956\u7ecf\u5386 2002 \u4eac\u90fd\u8d4f 2003 Lemelson\u2013MIT Prize 2006 Heinz Award in Technology, the Economy &amp; Employment 2009 Pittcon Heritage Award 2010 Kistler Prize 2011 National Medal of Science 2014 IEEE Medal for Innovations in Healthcare Technology 2019 \u79d1\u777f\u552f\u5b89\u5f15\u6587\u6842\u51a0\u5956 \u7814\u7a76\u4e1a\u7ee9 \u9ad8\u7075\u654f\u5ea6\u7684\u86cb\u767d\u8d28\u6d4b\u5e8f\u4eea\u7684\u5f00\u53d1 \u5f53Hood\u6559\u6388\u5f00\u59cb\u5728\u52a0\u5dde\u7406\u5de5\u5b66\u9662\u5b66\u4e60\u65f6\uff0c\u4ed6\u5c31\u8ba1\u5212\u5c06\u81ea\u5df1\u7684\u7814\u7a76\u751f\u6daf\u6295\u5165\u751f\u7269\u5b66\u548c\u4eea\u5668\u79d1\u6280\u9886\u57df\u3002\u81ea\u4ece\u7814\u7a76\u86cb\u767d\u8d28\u5316\u5b66\u4ee5\u6765\uff0c\u4ed6\u9996\u5148\u5bf9\u5229\u7528\u57c3\u5fb7\u66fc\u964d\u89e3\u6cd5\u6539\u826f\u86cb\u767d\u8d28\u6d4b\u5e8f\u4eea\u4ea7\u751f\u4e86\u5174\u8da3\u3002\u5c31\u5f53\u65f6\u7684\u6280\u672f\u800c\u8a00\uff0c\u6bcf\u6b21\u5206\u6790\u9700\u8981\u5927\u91cf\u6837\u54c1\uff08\u6beb\u514b\u7ea7\uff09\u3001Hood\u6559\u6388\u8fdb\u884c\u4e86\u5982\u4e0b\u6539\u826f\uff081\uff09\u901a\u8fc7\u5728\u6c14\u76f8\u800c\u4e0d\u662f\u6db2\u76f8\u4e2d\u8fdb\u884c\u57c3\u5fb7\u66fc\u964d\u89e3\u6765\u51cf\u5c11\u6837\u54c1\u635f\u5931\u3001\uff082\uff09\u5f00\u53d1\u4e86\u53ef\u4ee5\u627f\u53d7\u8150\u8680\u6027\u57c3\u5fb7\u66fc\u8bd5\u5242\u7684\u9600\u95e8\u3001\u4ece\u800c\u5f00\u53d1\u4e86\u9ad8\u7075\u654f\u7684\u6c14\u76f8\u6db2\u76f8\u86cb\u767d\u6d4b\u5e8f\u4eea\uff0c\u53ef\u5c06\u6240\u9700\u6837\u54c1\u91cf\u51cf\u5c11\u81f3\u5fae\u514b\u7ea7\uff081982\u5e74\uff09\u3002 DNA\u30fb\u80bd\u81ea\u52a8\u5408\u6210\u4eea\u7684\u5f00\u53d1 \u5927\u7ea6\u57281970\u5e74\u4ee3\u672b\uff0c\u79d1\u7f57\u62c9\u591a\u5927\u5b66\u7684Marvin Caruthers\u6559\u6388\u5f00\u53d1\u4e86\u4e00\u79cd\u7528\u4e8eDNA\u7684\u5316\u5b66\u5408\u6210\u65b9\u6cd5\uff08\u4e9a\u78f7\u9170\u80fa\u6cd5\uff09\u3002\u3002\u5bf9\u5f00\u53d1\u81ea\u52a8\u5316DNA\u5408\u6210\u4eea\u611f\u5174\u8da3\u7684Hood\u6559\u6388\u8bf7Caruthers\u6559\u6388\u8bb2\u6388\u4e9a\u78f7\u9170\u80fa\u6cd5\uff0c\u5e76\u901a\u8fc7\u5c06Caruthers\u6559\u6388\u5f00\u53d1\u7684\u53cd\u5e94\u8fdb\u884c\u81ea\u52a8\u5316\u6765\u5f00\u53d1\u81ea\u52a8\u5316DNA\u5408\u6210\u4eea\uff081983\uff09\u3002\u6b64\u5916\uff0c\u4ed6\u5229\u7528\u5728\u86cb\u767d\u8d28\u6d4b\u5e8f\u4eea\u548cDNA\u5408\u6210\u4eea\u65b9\u9762\u7684\u5f00\u53d1\u7ecf\u9a8c\u5f00\u53d1\u4e86\u80bd\u5408\u6210\u4eea\uff081984\u5e74\uff09\u3002 DNA\u81ea\u52a8\u6d4b\u5e8f\u4eea\u7684\u5f00\u53d1 1975\u5e74\u3001Walter Gilbert\u6559\u6388\u30fbFrederick Sanger\u6559\u6388\u3001\u5206\u522b\u5f00\u53d1\u4e86\u4f7f\u7528\u5316\u5b66\u964d\u89e3\u00b7\u9176\u7684DNA\u6d4b\u5e8f\u65b9\u6cd5\uff081980\u5e74\u3001\u8bfa\u8d1d\u5c14\u5316\u5b66\u5956\uff09\u3002Hood\u7b49\u4eba\u5bf9\u8fd9\u79cd\u65b9\u6cd5\u7684\u81ea\u52a8\u5316\u5f88\u611f\u5174\u8da3\uff0c\u5e76\u6839\u636eSanger\u6559\u6388\u62a5\u9053\u7684\u65b9\u6cd5\uff08\u53cc\u8131\u6c27\u6cd5\/ Sanger\u6cd5\uff09\u8fdb\u884c\u4e86DNA\u6d4b\u5e8f\u4eea\u7684\u5f00\u53d1\u3002 \u56fe1. \u8367\u5149\u6807\u8bb0\u7684\u53cc\u8131\u6c27\u6838\u82f7\u9178\u4e09\u78f7\u9178\uff08Dye-ddNTP\uff09\u3002 \u5728Hood\u6559\u6388\u7684\u65b9\u6cd5\u4e2d\uff0c\u5c06\u5c11\u91cf\u8367\u5149\u6807\u8bb0\u7684\u53cc\u8131\u6c27\u6838\u82f7\u9178\u4e09\u78f7\u9178\uff08ddNTP\uff09\u6df7\u5408\u5728DNA\u6269\u589e\u6eb6\u6db2\u4e2d\uff08\u56fe1\uff09\u3002\u7531\u4e8eddNTP\u57283&#8217;\u4f4d\u6ca1\u6709\u7f9f\u57fa\uff0c\u56e0\u6b64\u5f53\u901a\u8fc7DNA\u805a\u5408\u9176\u63ba\u5165\u65f6\uff0cDNA\u5ef6\u4f38\u5728\u90a3\u91cc\u505c\u6b62\uff08\u56fe2\uff09\u3002 \u56fe2. Hood\u7b49\u4eba\u7684DNA\u6d4b\u5e8f\u4eea\u673a\u5236\u3002 \u4ece\u8fd9\u79cd\u53cd\u5e94\u6eb6\u6db2\u83b7\u5f97\u7684\u4ea7\u7269\u662f\u5728\u5404\u4e2a\u4f4d\u7f6e\u7247\u6bb5\u5316\u7684DNA\u4ea7\u7269\u3002\u901a\u8fc7\u6bdb\u7ec6\u7ba1\u7535\u6cf3\u6839\u636e\u957f\u5ea6\u5c06\u5176\u5206\u79bb\uff0c\u5e76\u8bfb\u53d6\u4e0e\u6bcf\u4e2a\u78b1\u57fa\u76f8\u5bf9\u5e94\u7684\u8367\u5149\u4fe1\u53f7\u4ee5\u786e\u5b9a\u6a21\u677fDNA\u7684\u5e8f\u5217\u3002Sanger\u6559\u6388\u7b49\u4eba\u5f00\u53d1\u4e86ddNTP\u6cd5\uff08\u8131\u6c27\u6cd5\/ Sanger\u6cd5\uff09\uff0c\u4f46\u4ed6\u4eec\u7684\u65b9\u6cd5\u4f7f\u7528\u4e86\u653e\u5c04\u6027\u540c\u4f4d\u7d20\u6807\u8bb0\uff0c\u5e76\u5728\u6bcf\u6b21\u53cd\u5e94\u4e2d\u6dfb\u52a0\u4e86\u56db\u4e2addNTP\u4e2d\u7684\u4e00\u4e2a\uff0c\u4e5f\u5c31\u662f\u8bf4\uff0c\u6709\u5fc5\u8981\u5206\u522b\u8fdb\u884c\u56db\u4e2a\u53cd\u5e94\u3002\u4f5c\u4e3aSanger\u65b9\u6cd5\u7684\u6539\u8fdb\u7248\u672c\uff0cHood\u7b49\u4eba\u65b9\u6cd5\u662f\u7528\u56db\u79cd\u4e0d\u540c\u7684\u8367\u5149\u67d3\u6599\u6807\u8bb0ddNTP\uff0c\u7136\u540e\u540c\u65f6\u6dfb\u52a0\u5b83\u4eec\uff0c\u4ece\u800c\u4e00\u6b21\u6027\u53ef\u4ee5\u5728\u5355\u4e2a\u6bdb\u7ec6\u7ba1\u4e2d\u5206\u79bb\u548c\u5206\u6790\u4ea7\u7269\u3002\u00a0\u6b64\u5916\uff0c\u8fd8\u514b\u670d\u4e86\u81ea\u52a8\u5316\u65b9\u9762\u7684\u5404\u79cd\u56f0\u96be\uff0c\u5e76\u4e8e1986\u5e74\u6210\u529f\u5f00\u53d1\u4e86\u81ea\u52a8DNA\u6d4b\u5e8f\u4eea\u3002 \u52a0\u5dde\u7406\u5de5\u5b66\u9662\u7684Mike Hunkapiller\u535a\u58eb\u548cLloyd Smith\u535a\u58eb\uff08\u73b0\u4e3a\u5a01\u65af\u5eb7\u661f\u5927\u5b66\u6559\u6388\uff09\u90fd\u53c2\u4e0e\u4e86\u8be5\u7cfb\u5217\u8bbe\u5907\u7684\u5f00\u53d1\u3002\u6b64\u5916\uff0c\u8fd9\u4e9b\u8bbe\u5907\u901a\u8fc7\u4e00\u5bb6\u540d\u4e3aApplied Biosystems\u7684\u516c\u53f8\u8fdb\u884c\u4e86\u5546\u4e1a\u5316\uff0c\u5e76\u5e7f\u4e3a\u666e\u53ca\u3002 &nbsp; \u5176\u4ed6 \u5f53Hood\u6559\u6388\u4e0eCaruthers\u6559\u6388\u8c08\u8bba\u81ea\u52a8DNA\u5408\u6210\u4eea\u65f6\uff0cCaruthers\u6559\u6388\u60f3\u77e5\u9053\u4e3a\u4ec0\u4e48Hood\u6559\u6388\u4f1a\u8d39\u5fc3\u5730\u4f7f\u6838\u9178\u5408\u6210\u81ea\u52a8\u5316\u3002\u56e0\u4e3a\u5f53\u65f6\uff0c\u57fa\u56e0\u7ec4\u5b66\u548c\u86cb\u767d\u8d28\u7ec4\u5b66\u9886\u57df\u8fd8\u6ca1\u6709\u5f97\u5230\u53d1\u5c55\uff0c\u56e0\u6b64\uff0c\u4eba\u4eec\u5bf9\u6838\u9178\u5408\u6210\u4eea\u7684\u9700\u6c42\u9884\u8ba1\u4e0d\u4f1a\u589e\u957f\u3002 &nbsp; \u76f8\u5173\u6587\u732e Hewick, R. M.; Hunkapiller, M. W.; Hood, [&hellip;]","protected":false},"author":5,"featured_media":29073,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","enabled":false},"version":2}},"categories":[212],"tags":[2433],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"https:\/\/assets.www.gsbet888.com\/uploads\/2019\/10\/2019-10-01_01-39-05.png","jetpack_shortlink":"https:\/\/wp.me\/p96op1-7yU","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/posts\/29072"}],"collection":[{"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/users\/5"}],"replies":[{"embeddable":true,"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/comments?post=29072"}],"version-history":[{"count":20,"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/posts\/29072\/revisions"}],"predecessor-version":[{"id":51387,"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/posts\/29072\/revisions\/51387"}],"wp:featuredmedia":[{"embeddable":true,"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/media\/29073"}],"wp:attachment":[{"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/media?parent=29072"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/categories?post=29072"},{"taxonomy":"post_tag","embeddable":true,"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/tags?post=29072"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}