{"id":30931,"date":"2020-03-25T08:10:20","date_gmt":"2020-03-25T00:10:20","guid":{"rendered":"\/\/www.gsbet888.com\/?p=30931"},"modified":"2020-03-22T23:11:02","modified_gmt":"2020-03-22T15:11:02","slug":"%e5%bd%93%e4%bd%bf%e7%94%a8nmr%e6%a3%80%e6%b5%8b%e5%87%ba%e9%97%ae%e9%a2%98%e6%97%b6","status":"publish","type":"post","link":"\/\/www.gsbet888.com\/%e5%8c%96%e5%ad%a6%e6%9d%82%e8%ae%b0\/2020\/03\/%e5%bd%93%e4%bd%bf%e7%94%a8nmr%e6%a3%80%e6%b5%8b%e5%87%ba%e9%97%ae%e9%a2%98%e6%97%b6.html","title":{"rendered":"\u5f53\u4f7f\u7528NMR\u68c0\u6d4b\u51fa\u73b0\u95ee\u9898\u65f6"},"content":{"rendered":"<p>\u672c\u6587\u6765\u81eaChem-Station\u65e5\u6587\u7248 <a href=\"https:\/\/www.chem-station.com\/blog\/2020\/02\/nmr.html\">NMR\u306e\u6e2c\u5b9a\u304c\u3046\u307e\u304f\u3044\u304b\u306a\u3044\u3068\u304d<\/a> \u4f5c\u8005 \u00a0<a href=\"https:\/\/www.chem-station.com\/author\/gakushi\">Gakushi<\/a><\/p>\n<p>\u7ffb\u8bd1\u6295\u7a3f \u70b8\u9e21 \u6821\u5bf9 Jiao Jiao<\/p>\n<p>\u4eca\u5929\u6765\u603b\u7ed3\u4e0b\u4f7f\u7528NMR\u65f6\u51fa\u73b0\u6545\u969c\u65f6\u7684\u5e94\u5bf9\u65b9\u6cd5\u3002\uff08\u56e0\u4e3a\u7b14\u8005\u7528\u7684\u662fBruker user\u516c\u53f8\u751f\u4ea7\u7684NMR\u4eea\u5668\u8bbe\u5907\uff0c\u5bf9JEOL\u516c\u53f8\u751f\u4ea7\u7684NMR\u4eea\u5668\u8bbe\u5907\u5e76\u4e0d\u4e86\u89e3\uff09<span style=\"color: #ff0000;\">\u76f4\u5230\u73b0\u5728\uff0cNMR\u8fd8\u662f\u4e00\u79cd\u4ef7\u683c\u6602\u8d35\u7684\u68c0\u6d4b\u4eea\u5668\u3002\u800c\u4e14\uff0c\u56e0\u4e3a\u4f7f\u7528\u9891\u7387\u5f88\u9ad8\uff0c\u4e00\u65e6\u5b83\u51fa\u73b0\u6545\u969c\uff0c\u5927\u5bb6\u90fd\u4f1a\u5f88\u5934\u75bc\u3002\u5982\u679c\u9047\u5230\u9ebb\u70e6\uff0c\u6bd4\u8d77\u81ea\u5df1\u57cb\u5934\u86ee\u5e72\u4e0d\u5982\u627e\u4e0b\u6280\u672f\u4eba\u5458\u9700\u6c42\u5e2e\u52a9\u3002<\/span><\/p>\n<h4><strong>\u6837\u54c1\u6eb6\u6db2\u91cf\u8db3\u591f\u5417\uff1f<\/strong><\/h4>\n<p>\u5982\u679c\u6837\u54c1\u6eb6\u6db2\u7684\u91cf\u592a\u5c11\uff0c\u7ebf\u5708\u6e29\u5ea6\u592a\u9ad8\uff0c\u8bbe\u5907\u5c31\u4f1a\u635f\u574f\u3002\u5982\u679c\u6837\u54c1\u91cf\uff08\u5373\u6eb6\u8d28\uff09\u592a\u5c11\uff0c\u4ecd\u60f3\u7cbe\u786e\u68c0\u6d4b\u5316\u5408\u7269\u7ed3\u6784\uff0c\u5c31\u8981\u4fdd\u8bc1\u6eb6\u5242\u5145\u8db3\uff08\u4e00\u5b9a\u8981\u4fdd\u8bc1\u53bb\u8026\u7ebf\u5708\u7684\u9ad8\u5ea6\u4f4e\u4e8e\u6837\u54c1\u6eb6\u6db2\u7684\u6db2\u9762\uff09\u3002\u5c3d\u53ef\u80fd\u4f7f\u7528<a href=\"http:\/\/www.shigemi.co.jp\/page07\">shigemi<\/a>\u6837\u54c1\u7ba1\uff08\u65e5\u672c\u4e00\u5bb6\u5316\u5b66\u5668\u68b0\u5236\u9020\u516c\u53f8\u7684\u4ea7\u54c1\uff09\u6216\u8005\u4f7f\u7528\u5177\u6709\u66f4\u597d\u7684\u78c1\u94c1\u548c\u63a2\u5934\u7684NMR\u8bbe\u5907\u3002\u5982\u679c\u957f\u65f6\u95f4\u7528\u4f4e\u6cb8\u70b9\u6eb6\u5242\u8fdb\u884c\u6052\u6e29\u68c0\u6d4b\u6216\u975e\u6052\u6e29\u68c0\u6d4b\uff0c\u6700\u597d\u7528\u8584\u819c\u5c06\u6837\u54c1\u7ba1\u76d6\u5b50\u5305\u88f9\u8d77\u6765\u3002<\/p>\n<h4><strong>\u6837\u54c1\u7684\u6d53\u5ea6\u4e3a\u591a\u5c11\u5408\u9002\u5462\uff1f<\/strong><\/h4>\n<p>\u5982\u679c\u6837\u54c1\u6d53\u5ea6\u5f88\u4f4e\uff0c\u8981\u6ce8\u610f\u5728\u81ea\u52a8\u53d6\u6837\u5668\u4e2d\u4f7f\u7528\u7684\u81ea\u52a8\u9501\u573a\u548c\u5300\u573a\u53ef\u80fd\u4f1a\u53d1\u751f\u610f\u5916\u3002\u5982\u679c\u6837\u54c1\u6d53\u5ea6\u6781\u5176\u5730\u4f4e\u7684\u8bdd\uff0c\u8fd8\u53ef\u4ee5\u624b\u52a8\u8c03\u8282\u9501\u573a\u548c\u5300\u573a\u3002\uff08\u5176\u539f\u56e0\u662f\uff0cNMR\u548cMS\u4e0d\u540c\uff0cNMR\u662f\u4e00\u79cd\u7075\u654f\u5ea6\u975e\u5e38\u4f4e\u7684\u6d4b\u91cf\u65b9\u6cd5\uff0c\u9700\u8981\u5927\u91cf\u7684\u6837\u54c1\uff0c\u5982\u679c\u6837\u54c1\u91cf\u5f88\u5c11\uff0cNS(\u626b\u63cf\u6b21\u6570)\u5fc5\u987b\u8fbe\u5230128\u6b21\u4ee5\u4e0a\uff0c\u90a3\u6837\u4e5f\u9700\u8981\u5927\u91cf\u7684\u65f6\u95f4\u3002\u5982\u679c\u5316\u5408\u7269\u7684\u5b9a\u91cf\u4f9b\u5e94\u4e0d\u662f\u95ee\u9898\uff0c\u4e0d\u8981\u541d\u556c\uff0c\u8981\u4f7f\u7528\u8db3\u591f\u7684\u91cf\u8fdb\u884c\u6d4b\u91cf\u3002\u4f8b\u5982\uff0c\u8fdb\u884c\u78b3\u8c31\u68c0\u6d4b\u65f6\uff0c\u5982\u679c\u670920\u6beb\u514b\u523030\u6beb\u514b\u7684<sup>13<\/sup>C\u8bdd\uff0c\u6d4b\u91cf\u5f88\u5feb\u5c31\u4f1a\u7ed3\u675f\u3002\uff09<\/p>\n<p>\u53e6\u4e00\u4e2a\u65b9\u9762\u6765\u8bf4\uff0c\u6837\u54c1\u6d53\u5ea6\u592a\u9ad8\uff0c\u90a3\u4e48\u6837\u54c1\u6eb6\u6db2\u7c98\u5ea6\u5c31\u4f1a\u589e\u52a0\uff0c\u6837\u54c1\u6eb6\u6db2\u5c31\u4f1a\u5f88\u5bb9\u6613\u53d8\u7684\u4e0d\u5747\u4e00\uff0c\u56e0\u5c40\u90e8\u78c1\u573a\u7684\u73af\u5883\u88ab\u6270\u4e71\u4e86\uff0c\u4fe1\u53f7\u5cf0\u4f1a\u53d8\u5bbd\u3002\u8fd9\u79cd\u60c5\u51b5\u4e0b\uff0c\u9700\u8981\u8ddfNMR\u6280\u672f\u4eba\u5458\u786e\u5b9a\u662f\u66f4\u6362\u6eb6\u5242\uff0c\u8fd8\u662f\u964d\u4f4e\u6837\u54c1\u6d53\u5ea6\uff0c\u6291\u6216\u662f\u5347\u9ad8\u68c0\u6d4b\u6e29\u5ea6\u3002<\/p>\n<h4><strong>\u6ca1\u6709\u6c89\u6dc0\u7269\u5417\uff1f<\/strong><\/h4>\n<p>\u5982\u679c\u5f85\u6d4b\u6837\u54c1\u6eb6\u6db2\u6709\u6c89\u6dc0\u7269\uff0c\u5e94\u63d0\u524d\u8fc7\u6ee4\u4e00\u4e0b\u3002\u5982\u679cNMR\u5f85\u6d4b\u6eb6\u6db2\u6d51\u6d4a\u7684\u8bdd\uff0c\u5c31\u65e0\u6cd5\u5f97\u5230\u5e72\u51c0\u7684\u56fe\u8c31\u3002\uff08\u5728\u4ee5\u524d\u7684\u5b9e\u9a8c\u5ba4\u505a\u7edc\u5408\u7269\u7814\u7a76\u7684\u65f6\u5019\uff0c\u7ecf\u5e38\u628a\u6d51\u6d4a\u7684\u6837\u54c1\u6eb6\u6db2\u62ff\u53bb\u505aNMR\u68c0\u6d4b\uff0c\u5f53\u65f6\u5185\u5fc3\u8fd8\u60f3\u5e94\u8be5\u6ca1\u4e8b\u5427\u3002\uff09<\/p>\n<h4><strong>\u6837\u54c1\u7ba1\u6ca1\u6709\u95ee\u9898\u5417\uff1f<\/strong><\/h4>\n<p><a href=\"https:\/\/assets.www.gsbet888.com\/uploads\/2020\/03\/1-11.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-30932 \" src=\"https:\/\/assets.www.gsbet888.com\/uploads\/2020\/03\/1-11.jpg\" alt=\"\" width=\"746\" height=\"113\" srcset=\"https:\/\/assets.www.gsbet888.com\/uploads\/2020\/03\/1-11.jpg 660w, https:\/\/assets.www.gsbet888.com\/uploads\/2020\/03\/1-11-300x45.jpg 300w\" sizes=\"(max-width: 746px) 100vw, 746px\" \/><\/a><\/p>\n<p>\u5982\u679c\u6837\u54c1\u7ba1\u6709\u5f2f\u66f2\uff0c\u6216\u662f\u5f04\u810f\u4e86\u7684\u73b0\u8c61\u4f1a\u4f7f\u5c40\u90e8\u78c1\u573a\u73af\u5883\u4e0d\u5747\u5300\u5206\u5e03\uff0c\u8fd9\u6837\u7684\u8bdd\uff0c\u53ea\u80fd\u5c06\u6837\u54c1\u7ba1\u4e22\u5f03\u4e86\u3002\uff08\u5728\u68c0\u6d4b<sup>1<\/sup>H\u65f6\uff0c\u56e0\u4e3a\u6837\u54c1\u7ba1\u7684\u539f\u56e0\uff0c\u4fe1\u53f7\u4f1a\u4e0d\u5747\u5300\uff0c\u4f46spin\uff08\u65cb\u8f6c\uff09\u53ef\u4ee5\u6291\u5236\u4fe1\u53f7\u4e0d\u5747\u4e00\u7684\u73b0\u8c61\uff0c\u6240\u4ee5\u6211\u5e76\u4e0d\u5efa\u8bae\u4f60\u5728\u6d4b\u91cf\u6c22\u8c31\u65f6\u56e0\u4e3a\u6837\u54c1\u7ba1\u5f2f\u66f2\u800c\u5c06\u6837\u54c1\u7ba1\u4e22\u5f03\uff09\u518d\u6709\uff0c\u4e3a\u4e86\u9632\u6b62\u6837\u54c1\u7ba1\u53d1\u751f\u5f2f\u66f2\uff0c\u5e72\u71e5\u6837\u54c1\u7ba1\u7684\u65f6\u5019\u5e94\u8be5\u907f\u514d\u70ed\u70d8\u5e72\uff0c\u8bf7\u5728\u9ad8\u771f\u7a7a\u73af\u5883\u4e0b\u5e72\u71e5\u3002<\/p>\n<h4><strong>\u4e00\u5b9a\u9700\u8981\u9501\u573a\u5417\uff1f<\/strong><\/h4>\n<p>\u5982\u679c\u6ce2\u5cf0\u51fa\u73b0\u5728\u5085\u7acb\u53f6\u53d8\u6362\u540e\u9762\uff0c\u662f\u56e0\u4e3a\u9501\u573a\u7684\u539f\u56e0\u3002\u7279\u522b\u662f\u5728\u6709CD<sub>3<\/sub>OD\u4f5c\u4e3a\u6eb6\u5242\u7684\u65f6\u5019\uff0cAuto Lock\uff08\u81ea\u52a8\u9501\u573a\uff09\u96be\u4ee5\u53d1\u6325\u4f5c\u7528\u3002\u8fd9\u4e2a\u65f6\u5019\uff0c\u5c31\u8981\u4f7f\u7528\u624b\u52a8\u9501\u573a\u3002<\/p>\n<h4><strong>\u4e00\u5b9a\u8981\u5300\u573a\u5417\uff1f<\/strong><\/h4>\n<p>\u9700\u8981\u7cbe\u786e\u68c0\u6d4b\u65f6\uff0c\u5728\u5300\u573a\u81ea\u52a8\u8c03\u8282\u540e\uff0c\u53ef\u80fd\u9700\u8981\u624b\u52a8\u518d\u6b21\u8c03\u8282\u5300\u573a\u3002\u518d\u6709\uff0c\u5982\u679c\u6837\u54c1\u6d53\u5ea6\u592a\u4f4e\uff0c\u5300\u573a\u4f1a\u51cf\u5f31\u3002\uff08Bruker\u7684\u5206\u5149\u8ba1\u53d8\u6210\u4e86Avance Neo\u540e\uff0c\u81ea\u52a8\u5300\u573a\u7684\u7cbe\u5ea6\u53d8\u9ad8\u4e86\uff0c\u8fd9\u6837\u539f\u672c\u914d\u5957\u7684BSMS keyboard\u5c31\u7528\u4e0d\u5230\u4e86\uff0c\u6539\u4e3a\u5728\u5c4f\u5e55\u4e0a\u63a7\u5236\u3002\u56e0\u6b64\uff0c\u5fc5\u987b\u5728\u5c4f\u5e55\u4e0a\u8c03\u8282Manual Shin(\u624b\u52a8\u5300\u573a)\uff0c\u8fd9\u5c06\u4f1a\u53d8\u5f97\u5f88\u9ebb\u70e6\u3002\u4e3a\u4e86\u5f25\u8865\u81ea\u52a8\u5300\u573a\u8c03\u8282\u7684\u4e0d\u65b9\u4fbf\uff0c\u81ea\u52a8\u5300\u573a\u8c03\u8282\u540e\u53ef\u624b\u52a8\u5fae\u8c03\u5300\u573a\u3002\uff09<\/p>\n<p>\u5982\u679c\u5300\u573a\u4e0d\u5339\u914d\u7684\u65f6\u5019\uff0c\u53ef\u4ee5\u91cd\u65b0\u8bfb\u53d6\u5b9a\u671f\u4fdd\u5b58\u7684\u5300\u573a\u6587\u4ef6\u3002<\/p>\n<h4><strong>\u63a2\u5934\u8c03\u8c10\u4e86\u5417\uff1f<\/strong><\/h4>\n<p>\u63a2\u5934\u662f\u5426\u8c03\u8c10\u5bf9<sup>13<\/sup>C-NMR\u662f\u5f88\u91cd\u8981\u7684\u3002\u800c\u4e14\uff0c\u5982\u679c\u6ca1\u6709\u8c03\u8c10\u597d\u7684\u8bdd\uff0cHSQC\u6216HMBC\u662f\u4e0d\u4f1a\u6709\u4fe1\u53f7\u5cf0\uff08Cross peak\uff09\uff08\u7b14\u8005\u4e5f\u4e00\u5ea6\u72af\u8fc7\u8fd9\u6837\u7684\u9519\u8bef\uff09<\/p>\n<h4><strong>\u6837\u54c1\u65e0\u6cd5\u51fa\u5cf0<\/strong><\/h4>\n<p>\u5982\u679c\u6837\u54c1\u6ca1\u6709\u51fa\u5cf0\uff0c\u53ef\u4ee5\u8003\u8651\u4e00\u4e0b\u51e0\u79cd\u539f\u56e0\uff1a\u7a7a\u6c14\u4e0d\u591f\u6d41\u901a\uff0c\u6ca1\u6709\u4f7f\u7528\u5408\u9002\u5f97\u5230\u6837\u54c1\u8f6c\u5b50\uff0c\u68c0\u6d4b\u8fc7\u7a0b\u4e2d\u7684\u64cd\u4f5c\u5931\u8bef\u7b49\u3002\u8fd9\u662f\u4e00\u5b9a\u4e0d\u8981\u8d38\u7136\u884c\u4e8b\uff0c\u5fc5\u8981\u7684\u65f6\u5019\u8fd8\u662f\u627e\u6280\u672f\u4eba\u5458\u5bfb\u6c42\u5e2e\u52a9\u5427\u3002\uff08\u4ece\u524d\uff0c\u6837\u54c1\u6ca1\u6709\u51fa\u5cf0\u7684\u65f6\u5019\uff0c\u6309BSMS\u7684\u952e\u76d8\u540e\u9762\u7684\u590d\u4f4d\u952e\u4f3c\u4e4e\u6709\u4e9b\u4f5c\u7528\uff09<\/p>\n<h4><strong>\u4f7f\u7528\u6807\u51c6\u6837\u54c1\u68c0\u6d4b<\/strong><\/h4>\n<p>\u65e0\u8bba\u600e\u4e48\u505a\uff0c\u8fd8\u662f\u68c0\u6d4b\u4e0d\u4e86\u7684\u8bdd\uff0c\u7528\u6807\u51c6\u6837\u54c1\u505aNMR\u68c0\u6d4b\u4e00\u4e0b\u8bd5\u8bd5\u3002\u5982\u679c\u5373\u4f7f\u8fd9\u6837\u4e5f\u4e0d\u80fd\u51fa\u5cf0\uff0c\u90a3\u53ea\u597d\u548c\u627e\u6280\u672f\u4eba\u5458\u5bfb\u6c42\u5e2e\u52a9\u4e86\u3002<\/p>\n<h4><strong>\u4f7f\u7528\u4e86\u5408\u9002\u7684<\/strong><strong>NMR<\/strong><strong>\u8bbe\u5907\u5417\uff1f<\/strong><\/h4>\n<p>\u4ee5\u524d\u7684\u6587\u7ae0\u4e5f\u6709\u8fc7\u4ecb\u7ecd\uff0c\u4f7f\u7528\u88c5\u5907\u4e86Inverse Probe\uff08BBI\u3001TXI\uff09\u7684\u8bbe\u5907\u6765\u505a\u78b3\u8c31\uff0c\u8fc7\u4e86\u5f88\u957f\u65f6\u95f4\u4e5f\u6ca1\u6709\u53cd\u5e94\u3002\u5982\u679c\u8981\u5f97\u5230\u9ad8\u7cbe\u5ea6\u7684\u78b3\u8c31\u56fe\u63a8\u8350\u4f7f\u7528\u88c5\u914d\u6709TCI\u6216BBO\u7684NMR\u8bbe\u5907\u6216\u662fCryo\u3002\u4e00\u65b9\u9762\u6765\u8bf4\uff0c\u5982\u679c\u67092\u4e2a\u91cd\u6c22\u539f\u5b50\uff08D\uff09\u7684\u8bdd\uff0cBBI\u6216TXI\u4ecd\u7136\u6709\u6548\uff0c\u8981\u60f3\u5f97\u5230\u597d\u7684\u8c31\u56fe\uff0c\u4e0d\u4ec5\u4ec5\u8981\u4fdd\u8bc1\u78c1\u4f53\u8db3\u591f\u5927\uff0c\u8fd8\u8981\u4f7f\u7528\u5408\u9002\u7684\u63a2\u5934\u3002<\/p>\n<p>\u518d\u6709\uff0c\u5982\u679c\u6d4b\u91cf\u8c31\u56fe\u548c\u6807\u51c6\u8c31\u56fe\u7684\u8026\u5408\u5cf0\u6709\u5dee\u5f02\uff0c\u5982\u679c\u8981\u6b63\u786e\u5730\u663e\u793a\u51fa\u8026\u5408\u5cf0\uff0c\u6700\u597d\u4f7f\u7528\u540c\u6837\u5c3a\u5bf8\u7684\u78c1\u4f53\u3002<\/p>\n<h4><strong>\u6807\u51c6\u503c\u548c\u68c0\u6d4b\u503c\u6709\u5dee\u5f02\u600e\u4e48\u529e\uff1f<\/strong><\/h4>\n<p><span style=\"color: #ff0000;\"><strong>\u6eb6\u5242\u7684\u79cd\u7c7b<\/strong><\/span><\/p>\n<p><a href=\"https:\/\/assets.www.gsbet888.com\/uploads\/2020\/03\/2-10.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-30933 \" src=\"https:\/\/assets.www.gsbet888.com\/uploads\/2020\/03\/2-10.jpg\" alt=\"\" width=\"752\" height=\"114\" srcset=\"https:\/\/assets.www.gsbet888.com\/uploads\/2020\/03\/2-10.jpg 660w, https:\/\/assets.www.gsbet888.com\/uploads\/2020\/03\/2-10-300x45.jpg 300w\" sizes=\"(max-width: 752px) 100vw, 752px\" \/><\/a><\/p>\n<p>\u68c0\u6d4b\u6240\u7528\u7684\u6eb6\u5242\u4e00\u6837\u5417\uff1f\u6211\u4eec\u5f88\u5bb9\u6613\u5ffd\u89c6\u4e0d\u540c\u6eb6\u5242\u95f4\u7684\u5dee\u522b\u3002\u5728\u68c0\u6d4b\u4e4b\u524d\uff0c\u4e00\u5b9a\u8981\u786e\u8ba4\u4f7f\u7528\u7684\u6eb6\u5242\u548c\u53c2\u8003\u6587\u732e\u4e2d\u7684\u6eb6\u5242\u662f\u5426\u4e00\u6837\u3002<\/p>\n<p><span style=\"color: #ff0000;\"><strong>pH<\/strong><strong>\u503c<\/strong><\/span><\/p>\n<p>\u80fa\u7c7b\u548c\u7fa7\u9178\u4f5c\u4e3a\u6eb6\u5242\u7684\u8bdd\uff0c\u8c31\u56fe\u4f1a\u548c\u6807\u51c6\u8c31\u56fe\u6709\u51fa\u5165\u7684\u5316\u5408\u7269\uff0c\u5148\u5224\u65ad\u662f\u5426\u4e3a\u5176\u76f8\u5e94\u7684\u76d0\u4e86\uff0c\u5982\u679c\u662f\u76d0\u7684\u5316\u5408\u7269\u7684\u8bdd\uff0c\u68c0\u67e5\u5b83\u4eec\u7684\u79cd\u7c7b\u662f\u5426\u76f8\u540c\u3002\uff08\u5982\u80fa\u7c7b\u5316\u5408\u7269\uff0c\u50cfTFA\u4e09\u6c1f\u4e59\u9178\u76d0\uff0cFA\u6c1f\u4e59\u9178\u76d0\uff0c\u76d0\u9178\u76d0\uff0c\u5bf9\u7532\u82ef\u78fa\u9178\u76d0\uff0c\u6839\u636e\u4ed6\u4eec\u6240\u5e26\u7684\u8d1f\u79bb\u5b50\u4e0d\u540c\uff0c\u5316\u5b66\u4f4d\u79fb\u503c\u4e5f\u4f1a\u6709\u5dee\u5f02\u3002\u5982\u679c\u6ca1\u6709\u6807\u51c6\u8c31\u56fe\uff0c\u8981\u786e\u8ba4\u4e00\u4e0b\u6837\u54c1\u7eaf\u5316\u6761\u4ef6\uff08\u7528RP-HPLC\u53cd\u76f8\u9ad8\u6548\u6db2\u76f8\u8272\u8c31\u68c0\u9a8c\uff09\u6216\u7ed3\u6676\u6761\u4ef6\uff09\u3002\u518d\u6709\uff0c\u4e5f\u8981\u68c0\u67e5\u6837\u54c1\u6eb6\u6db2\u7684pH\u503c\u662f\u5426\u76f8\u540c\u3002\u6bd4\u5982\uff0c\u5373\u4f7f\u90fd\u662f\u6c1f\u4e59\u9178\u76d0\uff0cpH\u503c\u4e0d\u540c\uff0c\u4fe1\u53f7\u5cf0\u7684\u5316\u5b66\u4f4d\u79fb\u503c\u53c8\u4f1a\u4e0d\u540c\u3002<\/p>\n<p><span style=\"color: #ff0000;\"><strong>\u68c0\u6d4b\u6d53\u5ea6<\/strong><\/span><\/p>\n<p>\u4e00\u5b9a\u8981\u786e\u8ba4\u68c0\u6d4b\u6d53\u5ea6\u662f\u5426\u548c\u6587\u732e\u91cc\u7684\u4e00\u6837\u3002\u5982\u679c\u6d53\u5ea6\u76f8\u5dee\u592a\u5927\uff0c\u6d53\u5ea6\u4e5f\u4f1a\u968f\u7740\u5316\u5408\u7269\u5f62\u6210\u4e8c\u805a\u4f53\u800c\u53d1\u751f\u53d8\u5316\u3002\uff08\u56e0\u6b64\uff0c\u5728\u5929\u7136\u5316\u5408\u7269\u5206\u79bb\u6587\u732e\u4e2d\uff0c\u5bf9\u5316\u5408\u7269\u7ed3\u6784\u8fdb\u884c\u7ed3\u6784\u68c0\u6d4b\u65f6\uff0c\u6587\u732e\u4e2d\u5e38\u5e38\u4f1a\u9644\u5e26\u8bb0\u8f7d\u7740\u68c0\u6d4b\u65f6\u7684\u68c0\u6d4b\u6d53\u5ea6\u3002\uff09<\/p>\n<p><span style=\"color: #ff0000;\"><strong>NMR<\/strong><strong>\u8c31\u56fe\u4fe1\u53f7\u6742\u4e71<\/strong><\/span><\/p>\n<p>\u6709\u7684\u5408\u6210\u7684\u5316\u5408\u7269\u5728CDCl<sub>3<\/sub>\u6eb6\u6db2\u4e2d\u4e0d\u7a33\u5b9a\uff0c\u6bd4\u5982\u4e59\u9170\u919a\u3001\u4e8c\u7ea7\u4e59\u919a\u3001\u4e59\u9187\u919a\u7b49\u5728\u5fae\u91cf\u7684\u9178\u4e2d\u4e0d\u7a33\u5b9a\uff0c\u7ecf\u5e38\u5728\u68c0\u6d4b\u524d\u6216\u68c0\u6d4b\u4e2d\u5206\u89e3\u3002\u5728\u8fd9\u79cd\u60c5\u51b5\u4e0b\uff0c\u6211\u4eec\u53ef\u4ee5\u7528C<sub>6<\/sub>D<sub>6<\/sub>\u3001toloene -d<sub>8<\/sub>\u6216DMSO-d<sub>6<\/sub>\u4f5c\u4e3a\u6eb6\u5242\u6765\u8fdb\u884c\u68c0\u6d4b\u3002\u6216\u8005\uff0c\u9884\u5148\u7528\u78b1\u6027\u6c27\u5316\u94dd\u586b\u5145\u7684\u6ef4\u7ba1\u5fae\u67f1\u5c06CDCl<sub>3<\/sub>\u4e2d\u7684\u9178\u53bb\u9664\u6765\u4f7f\u5f85\u6d4b\u5316\u5408\u7269\u5728CDCl<sub>3<\/sub>\u4e2d\u7a33\u5b9a\u3002\u8fd8\u6709\u4e00\u79cd\u65b9\u6cd5\uff0c\u5c31\u662f\u957f\u65f6\u95f4\u68c0\u6d4b\uff08<sup>13<\/sup>C\u62162D\uff09\u540e\u518d\u4e00\u6b21\u68c0\u6d4b<sup>1<\/sup>H\uff08\u626b\u63cf\u6b21\u6570\u4e3a2\uff09\uff0c\u8fd9\u6837\u53ef\u4ee5\u5f88\u8f7b\u677e\u5730\u5224\u522b\u51faNMR\u68c0\u6d4b\u7684\u5316\u5408\u7269\u6709\u6ca1\u6709\u5206\u89e3\u3002<\/p>\n<p>\u5728\u4ee5\u540e\u7684\u6587\u7ae0\u4e2d\u4f1a\u63d0\u5230\uff0c\u4ef2\u80fa\uff0c\u73af\u70f7\u70c3\uff08\u62e5\u67098-11\u4e2a\u78b3\u539f\u5b50\uff09\u5316\u5408\u7269\u548c\u975e\u624b\u6027\u5316\u5408\u7269\u7b49\u5bb9\u6613\u53d8\u6210\u65cb\u8f6c\u5f02\u6784\u4f53\uff0c\u5728NMR\u68c0\u6d4b\u65f6\uff0c\u5728\u4e00\u5b9a\u7684\u4fe1\u53f7\u6bd4\u7387\u4e0b\uff0c\u4e00\u4e2a\u4fe1\u53f7\u5cf0\u4f1a\u5206\u6210\u4e24\u4e2a\u3002\u8fd9\u79cd\u60c5\u51b5\u4e0b\uff0c\u9700\u8981\u4f7f\u7528\u53d8\u6e29NMR\u8fdb\u884c\u68c0\u6d4b\u3002<\/p>\n<p><span style=\"color: #ff0000;\"><strong>\u4fe1\u566a\u6bd4\u4e0d\u4f73<\/strong><\/span><\/p>\n<p>\u6709\u65f6\u5019\u56e0\u4e3a\u68c0\u6d4b\u73af\u5883\u6216\u4e4b\u524d\u6837\u54c1\u68c0\u6d4b\u3001\u8bbe\u5b9a\u548c\u4ee5\u5f80\u4e0d\u540c\uff0c\u53e6\u5916NMR\u63a2\u5934\u4f7f\u7528\u8fc7\u7a0b\u6162\u6162\u88ab\u6c61\u67d3\uff0c\u90fd\u4f1a\u4f7f\u4fe1\u566a\u6bd4\u53d8\u5f97\u4e0d\u4f73\u3002\uff08\u6211\u66fe\u8ba9NMR\u7684\u6280\u672f\u4eba\u5458\u7ed9\u6211\u770b\u8fc7\u5728\u6b63\u5e38\u7528\u4e866\u4e2a\u6708\u7684\u63a2\u5934\uff0c\u6bcf\u5929\u53ea\u8fdb\u884c20\u4e2a\u5de6\u53f3\u7684\u6837\u54c1\u7684\u6d4b\u91cf\uff0c\u4f46\u63a2\u5934\u4f9d\u7136\u76f8\u5f53\u810f\u3002\uff09\u5982\u679c\u89c9\u5f97\u5f88\u96be\u8c03\u8282\u7684\u8bdd\uff0c\u95ee\u6280\u672f\u4eba\u5458\u662f\u6700\u597d\u7684\u529e\u6cd5\u3002<\/p>\n<p>\u5728\u68c0\u6d4b\u6c22\u8c31\u65f6\uff0c\u6709\u65f6\u6211\u4eec\u53ef\u4ee5\u4e0d\u7528\u8003\u8651\u5cf0\u9762\u79ef\u7684\u79ef\u5206\u6bd4\uff0c\u4f7f\u7528\u7f29\u77ed\u68c0\u6d4b\u65f6\u95f4(aq) \u7684\u529e\u6cd5\u66f4\u4e3a\u7b80\u5355\u3002\u6211\u4eec\u53ef\u4ee5\u597d\u597d\u5229\u7528aq\uff0c\u4e0d\u8fc7\uff0c\u9700\u8981\u6ce8\u610f\u4e00\u822c\u7684\u8d28\u5b50\u7684\u5f1b\u8c6b\u65f6\u95f4T1\u662f0.5-4.0\uff0c\u5982\u679c\u68c0\u6d4b\u65f6\u95f4\u592a\u77ed\uff0c\u4fe1\u53f7\u5cf0\u7684\u79ef\u5206\u503c\u4f1a\u6d88\u5931\uff0c\u8fd9\u4e00\u70b9\u8981\u683c\u5916\u6ce8\u610f\u3002\uff08\u6211\u4e00\u8fb9\u8bbe\u5b9aaq\u4e3a3.0\uff0c\u518d\u77ed\u4e00\u70b9\u7684\u8bdd\u662f2.0\uff0c\u4e0d\u8fc7\u6211\u4e2a\u4eba\u7684\u610f\u89c1\u662f\u9009\u62e9\u5408\u9002\u7684NMR\u8bbe\u5907\u548c\u63a2\u5934\u800c\u4e0d\u662f\u901a\u8fc7\u7f29\u77edaq\u6765\u8c03\u8282\u4fe1\u566a\u6bd4\uff09<\/p>\n<p><span style=\"color: #ff0000;\"><strong><sub>\u00a0<\/sub><\/strong><sup>13<\/sup>C<strong>\u4e30\u5ea6\u592a\u4f4e<\/strong><\/span><\/p>\n<p>\u9996\u5148\u786e\u5b9a\u50cf\u53d4\u4e01\u57fa\u4e8c\u7532\u57fa\u7845\u57fa\u4e4b\u7c7b\uff0c\u76f8\u540c\u7684\u7532\u57fa\u662f\u5426\u6709\u5cf0\u91cd\u53e0\u7684\u73b0\u8c61\u3002<\/p>\n<p>\u56e0\u4e3a\u5b63\u78b3\u539f\u5b50\u7684\u5f1b\u8c6b\u65f6\u95f4\u5f88\u957f\uff0c\u5373\u4f7f\u65bd\u52a090\u4e2a\u5355\u4f4d\u7684\u8109\u51b2\uff0c\u4e5f\u9700\u8981\u8fc7\u5f88\u957f\u65f6\u95f4\u624d\u80fd\u5f97\u5230\u8c31\u56fe\uff0c\u56e0\u6b64\u5efa\u8bae\u628a\u5f1b\u8c6b\u65f6\u95f4\u53c2\u6570\u5ef6\u957f\uff0c\u7136\u540e\u518d\u6d4b\u91cf\u4e00\u6b21\u3002<\/p>\n<p>\u5f53\u68c0\u6d4b\u6c22\u8c31\u4fe1\u53f7\u5cf0\u53d8\u5bbd\u65f6\uff0c\u53ef\u80fd\u4e0d\u4f1a\u770b\u5230<sup>13<\/sup>C\u4fe1\u53f7\u5cf0\uff08\u5982\u679c\u7a0d\u5fae\u8c03\u6574\u4e0b\u53c2\u6570\uff0c<sup>13<\/sup>C\u7684\u4fe1\u53f7\u5cf0\u53ef\u80fd\u4f1a\u663e\u793a\u51fa\u6765\uff09\u3002\u9996\u5148\uff0c\u6539\u53d8NMR\u6eb6\u5242\u53ca\u5176\u6d53\u5ea6\uff0c\u8c03\u5242pH\u503c\uff0c\u5c3d\u53ef\u80fd\u4f7f<sup>1<\/sup>H\u7684\u4fe1\u53f7\u5cf0\u6e05\u6670\uff0c\u5219\u6709\u53ef\u80fd\u89c2\u6d4b\u5230<sup>13<\/sup>C\u4fe1\u53f7\u5cf0\u3002\u5982\u679c\u8fd8\u5f97\u4e0d\u5230\u6e05\u695a\u7684\u78b3\u539f\u5b50\u4fe1\u606f\uff0c\u53ef\u4ee5\u901a\u8fc7\u4f7f\u7528HSQC\u6216HMBC\u786e\u8ba4\u78b3\u539f\u5b50\u4fe1\u53f7\u5cf0\u3002<\/p>\n<p>\u5982\u679c\u78b3\u539f\u5b50\u9644\u8fd1\u5b58\u5728F\u539f\u5b50\uff0c\u90a3\u4e48\u78b3\u8c31\u4fe1\u53f7\u5cf0\u4f1a\u56e0\u4e3a\u8026\u5408\u53d1\u751f\u5206\u88c2\uff0c\u5206\u88c2\u4e3a\u5f88\u591a\u4e2a\u4fe1\u53f7\u5cf0\uff0c\u4fe1\u53f7\u5f3a\u5ea6\u4f1a\u51cf\u5f31\u3002\u4e3a\u4e86\u80fd\u6bd4\u8f83\u6e05\u6670\u5730\u6536\u96c6\u4fe1\u53f7\uff0c\u6700\u597d\u9009\u7528\u6d53\u5ea6\u9ad8\u7684\u6837\u54c1\u6eb6\u6db2\u3002\u53e6\u5916\uff0c\u7b14\u8005\u867d\u7136\u6ca1\u6709\u4f7f\u7528\u901a\u8fc7\u63d0\u9ad8\u6837\u54c1\u6d53\u5ea6\u7684\u65b9\u6cd5\uff0c\u4f46\u4f7f\u7528\u4e86<sup>19<\/sup>F\u6765\u8d77\u5230\u53bb\u8026\u7684\u4f5c\u7528\u3002<\/p>\n<p>\u76f4\u63a5\u4e0eB(\u787c)\u76f8\u8fde\u7684\u78b3\u539f\u5b50\u5728\u78b3\u8c31\u4e2d\u7ecf\u5e38\u65e0\u6cd5\u89c2\u6d4b\u5230\u5bf9\u5e94\u7684\u4fe1\u53f7\u5cf0\uff0c\u9700\u8981\u901a\u8fc7HMBC\u8c31\u56fe\u786e\u5b9a<sup>13<\/sup>C\u4f4d\u7f6e\u5e76\u4e14\u8981\u6539\u53d8\u6d4b\u91cf\u53c2\u6570\u3002\uff08\u7528\u4e8e\u5076\u8054\u53cd\u5e94\u7684\u787c\u9178\u4e2d\u6709sp<sup>2<\/sup>\u6742\u5316\u7684\u78b3\uff0c\u56e0\u6b64\u9700\u8981HMBC\u3002\u56e0\u4e3a\u7b14\u8005\u6ca1\u6709\u63a5\u89e6\u8fc7\u70f7\u57fa\u5316\u5408\u7269\uff0c\u6240\u4ee5\u4e0d\u77e5\u9053\u4f1a\u6709\u4ec0\u4e48\u6837\u7684\u53cd\u5e94\u3002\u5982\u679cB\u7ed3\u5408\u5728\u9971\u548c\u78b3\u4e0a\uff0c\u53ef\u4ee5\u7528\u66f4\u9ad8\u7075\u654f\u5ea6\u7684HSQC\u6765\u68c0\u6d4b\u78b3\u539f\u5b50\u662f\u5426\u5b58\u5728\u3002\uff09\u901a\u5e38\u60c5\u51b5\u4e0b\u662f\u5f88\u96be\u770b\u5230<sup>13<\/sup>C\u5bf9\u5e94\u7684\u4fe1\u53f7\u5cf0\u7684\uff0c\u7528SI\u548c<sup>11<\/sup>B-NMR\u65f6\uff0c\u5373\u4f7f\u770b\u4e0d\u5230<sup>13<\/sup>C\u7684\u5355\u4fe1\u53f7\u5cf0\u4e5f\u4e0d\u8981\u7d27\u3002\uff08\u56e0\u4e3a\u89c2\u6d4b\u5230\u78b3\u539f\u5b50\u7684\u4fe1\u53f7\u5cf0\u5f88\u96be\uff0c\u6240\u4ee5\u6587\u7ae0\u91cc\u91c7\u7528\u7684\u65b9\u6cd5\u53ea\u80fd\u9002\u7528\u4e8e\u5f53\u78b3\u539f\u5b50\u4fe1\u53f7\u5cf0\u5bf9\u7ed3\u679c\u5f71\u54cd\u4e0d\u5927\u7684\u65f6\u5019\u3002\uff09<\/p>\n<h4><strong>\u5176\u4ed6\u7684\u5efa\u8bae<\/strong><\/h4>\n<p><a href=\"https:\/\/assets.www.gsbet888.com\/uploads\/2020\/03\/3-8.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-30934 \" src=\"https:\/\/assets.www.gsbet888.com\/uploads\/2020\/03\/3-8.jpg\" alt=\"\" width=\"686\" height=\"104\" srcset=\"https:\/\/assets.www.gsbet888.com\/uploads\/2020\/03\/3-8.jpg 660w, https:\/\/assets.www.gsbet888.com\/uploads\/2020\/03\/3-8-300x45.jpg 300w\" sizes=\"(max-width: 686px) 100vw, 686px\" \/><\/a><\/p>\n<p>\u5e02\u9762\u4e0a\u6709\u5356\u7ec6\u7ec6\u957f\u957f\u7684NMR\u4e13\u7528\u7684\u73bb\u7483\u6ef4\u7ba1\u3002\u6211\u81ea\u5df1\u4e0d\u600e\u4e48\u7528\uff0c\u4f46\u53d6\u6837\u7684\u4eba\u7ecf\u5e38\u4f7f\u7528\u3002\u5f53\u6837\u54c1\u56de\u6536\u65f6\u4e0d\u597d\u53d6\u7684\u65f6\u5019\uff0c\u662f\u5426\u53ef\u4ee5\u8003\u8651\u4e70\u4e9b\u8fd9\u6837\u7684\u6ef4\u7ba1\u4f1a\u65b9\u4fbf\u4e9b\uff1f<\/p>\n<p>\u5b9e\u9a8c\u5ba4\u5e38\u5907\u81ea\u52a8\u53d6\u6837\u5668\uff0c\u907f\u514d\u68c0\u6d4b\u7684\u6837\u54c1\u5f88\u591a\u65f6\u5bb9\u6613\u5c06\u81ea\u5df1\u7684\u6837\u54c1\u548c\u5176\u4ed6\u4eba\u7684\u6837\u54c1\u5f04\u6df7\u3002\u6216\u8005\u4f7f\u7528\u4e0d\u540c\u989c\u8272\u7684\u6837\u54c1\u7ba1\u5e3d\u6216\u8005\u5728\u6837\u54c1\u7ba1\u5e3d\u4e0a\u5199\u4e0a\u81ea\u5df1\u540d\u5b57\u7684\u5927\u5199\u5b57\u6bcd\uff0c\u4ee5\u4fbf\u4e8e\u533a\u5206\u3002\uff08\u53e6\u5916\uff0c\u4e5f\u80fd\u964d\u4f4e\u88ab\u522b\u4eba\u62ff\u8d70\u6837\u54c1\u88ab\u9519\u6254\u7684\u98ce\u9669\u3002\uff09<\/p>\n<h4><strong>\u540e\u8bb0<\/strong><\/h4>\n<p><span style=\"color: #ff0000;\"><strong>\u6700\u540e\u518d\u8bf4\u4e00\u53e5\uff0c<\/strong><strong>NMR<\/strong><strong>\u662f\u5f88\u6602\u8d35\u7684\u4eea\u5668\u3002\u9047\u5230\u95ee\u9898\u65f6\uff0c\u9996\u5148\u8981\u51b7\u9759\u3002\u5982\u679c\u6ca1\u6709\u4fe1\u5fc3\u89e3\u51b3\u7684\u8bdd\uff0c\u6700\u597d\u6c42\u52a9\u4e8e\u6280\u672f\u4eba\u5458\u6216\u8005\u6307\u5bfc\u4eba\uff08\u81f3\u5c11\u4e5f\u5f97\u662f\u5b9e\u9a8c\u5ba4\u7684\u524d\u8f88\u5427\uff09\u3002<\/strong><\/span><\/p>\n<p>\u672c\u6587\u7248\u6743\u5c5e\u4e8e\u00a0<a href=\"\/\/www.gsbet888.com\/\">Chem-Station\u5316\u5b66\u7a7a\u95f4<\/a>\uff0c\u00a0\u6b22\u8fce\u70b9\u51fb\u6309\u94ae\u5206\u4eab\uff0c\u672a\u7ecf\u8bb8\u53ef\uff0c\u8c22\u7edd\u8f6c\u8f7d\uff01<\/p>\n","protected":false},"excerpt":{"rendered":"\u672c\u6587\u6765\u81eaChem-Station\u65e5\u6587\u7248 NMR\u306e\u6e2c\u5b9a\u304c\u3046\u307e\u304f\u3044\u304b\u306a\u3044\u3068\u304d \u4f5c\u8005 \u00a0Gakushi \u7ffb\u8bd1\u6295\u7a3f \u70b8\u9e21 \u6821\u5bf9 Jiao Jiao \u4eca\u5929\u6765\u603b\u7ed3\u4e0b\u4f7f\u7528NMR\u65f6\u51fa\u73b0\u6545\u969c\u65f6\u7684\u5e94\u5bf9\u65b9\u6cd5\u3002\uff08\u56e0\u4e3a\u7b14\u8005\u7528\u7684\u662fBruker user\u516c\u53f8\u751f\u4ea7\u7684NMR\u4eea\u5668\u8bbe\u5907\uff0c\u5bf9JEOL\u516c\u53f8\u751f\u4ea7\u7684NMR\u4eea\u5668\u8bbe\u5907\u5e76\u4e0d\u4e86\u89e3\uff09\u76f4\u5230\u73b0\u5728\uff0cNMR\u8fd8\u662f\u4e00\u79cd\u4ef7\u683c\u6602\u8d35\u7684\u68c0\u6d4b\u4eea\u5668\u3002\u800c\u4e14\uff0c\u56e0\u4e3a\u4f7f\u7528\u9891\u7387\u5f88\u9ad8\uff0c\u4e00\u65e6\u5b83\u51fa\u73b0\u6545\u969c\uff0c\u5927\u5bb6\u90fd\u4f1a\u5f88\u5934\u75bc\u3002\u5982\u679c\u9047\u5230\u9ebb\u70e6\uff0c\u6bd4\u8d77\u81ea\u5df1\u57cb\u5934\u86ee\u5e72\u4e0d\u5982\u627e\u4e0b\u6280\u672f\u4eba\u5458\u9700\u6c42\u5e2e\u52a9\u3002 \u6837\u54c1\u6eb6\u6db2\u91cf\u8db3\u591f\u5417\uff1f \u5982\u679c\u6837\u54c1\u6eb6\u6db2\u7684\u91cf\u592a\u5c11\uff0c\u7ebf\u5708\u6e29\u5ea6\u592a\u9ad8\uff0c\u8bbe\u5907\u5c31\u4f1a\u635f\u574f\u3002\u5982\u679c\u6837\u54c1\u91cf\uff08\u5373\u6eb6\u8d28\uff09\u592a\u5c11\uff0c\u4ecd\u60f3\u7cbe\u786e\u68c0\u6d4b\u5316\u5408\u7269\u7ed3\u6784\uff0c\u5c31\u8981\u4fdd\u8bc1\u6eb6\u5242\u5145\u8db3\uff08\u4e00\u5b9a\u8981\u4fdd\u8bc1\u53bb\u8026\u7ebf\u5708\u7684\u9ad8\u5ea6\u4f4e\u4e8e\u6837\u54c1\u6eb6\u6db2\u7684\u6db2\u9762\uff09\u3002\u5c3d\u53ef\u80fd\u4f7f\u7528shigemi\u6837\u54c1\u7ba1\uff08\u65e5\u672c\u4e00\u5bb6\u5316\u5b66\u5668\u68b0\u5236\u9020\u516c\u53f8\u7684\u4ea7\u54c1\uff09\u6216\u8005\u4f7f\u7528\u5177\u6709\u66f4\u597d\u7684\u78c1\u94c1\u548c\u63a2\u5934\u7684NMR\u8bbe\u5907\u3002\u5982\u679c\u957f\u65f6\u95f4\u7528\u4f4e\u6cb8\u70b9\u6eb6\u5242\u8fdb\u884c\u6052\u6e29\u68c0\u6d4b\u6216\u975e\u6052\u6e29\u68c0\u6d4b\uff0c\u6700\u597d\u7528\u8584\u819c\u5c06\u6837\u54c1\u7ba1\u76d6\u5b50\u5305\u88f9\u8d77\u6765\u3002 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keyboard\u5c31\u7528\u4e0d\u5230\u4e86\uff0c\u6539\u4e3a\u5728\u5c4f\u5e55\u4e0a\u63a7\u5236\u3002\u56e0\u6b64\uff0c\u5fc5\u987b\u5728\u5c4f\u5e55\u4e0a\u8c03\u8282Manual Shin(\u624b\u52a8\u5300\u573a)\uff0c\u8fd9\u5c06\u4f1a\u53d8\u5f97\u5f88\u9ebb\u70e6\u3002\u4e3a\u4e86\u5f25\u8865\u81ea\u52a8\u5300\u573a\u8c03\u8282\u7684\u4e0d\u65b9\u4fbf\uff0c\u81ea\u52a8\u5300\u573a\u8c03\u8282\u540e\u53ef\u624b\u52a8\u5fae\u8c03\u5300\u573a\u3002\uff09 \u5982\u679c\u5300\u573a\u4e0d\u5339\u914d\u7684\u65f6\u5019\uff0c\u53ef\u4ee5\u91cd\u65b0\u8bfb\u53d6\u5b9a\u671f\u4fdd\u5b58\u7684\u5300\u573a\u6587\u4ef6\u3002 \u63a2\u5934\u8c03\u8c10\u4e86\u5417\uff1f \u63a2\u5934\u662f\u5426\u8c03\u8c10\u5bf913C-NMR\u662f\u5f88\u91cd\u8981\u7684\u3002\u800c\u4e14\uff0c\u5982\u679c\u6ca1\u6709\u8c03\u8c10\u597d\u7684\u8bdd\uff0cHSQC\u6216HMBC\u662f\u4e0d\u4f1a\u6709\u4fe1\u53f7\u5cf0\uff08Cross peak\uff09\uff08\u7b14\u8005\u4e5f\u4e00\u5ea6\u72af\u8fc7\u8fd9\u6837\u7684\u9519\u8bef\uff09 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