{"id":32089,"date":"2020-05-20T08:00:32","date_gmt":"2020-05-20T00:00:32","guid":{"rendered":"\/\/www.gsbet888.com\/?p=32089"},"modified":"2020-05-13T21:58:56","modified_gmt":"2020-05-13T13:58:56","slug":"%e5%ae%9a%e9%87%8fpcr%ef%bc%88qpcr-quantitative-pcr%ef%bc%89%ef%bc%8c%e5%ae%9e%e6%97%b6%e8%8d%a7%e5%85%89%e5%ae%9a%e9%87%8fpcr","status":"publish","type":"post","link":"\/\/www.gsbet888.com\/%e5%8c%96%e5%ad%a6%e6%9d%82%e8%ae%b0\/2020\/05\/%e5%ae%9a%e9%87%8fpcr%ef%bc%88qpcr-quantitative-pcr%ef%bc%89%ef%bc%8c%e5%ae%9e%e6%97%b6%e8%8d%a7%e5%85%89%e5%ae%9a%e9%87%8fpcr.html","title":{"rendered":"\u5b9a\u91cfPCR\uff08qPCR ; quantitative PCR\uff09\uff0c\u5b9e\u65f6\u8367\u5149\u5b9a\u91cfPCR"},"content":{"rendered":"

\u672c\u6587\u6765\u81eaChem-Station\u65e5\u6587\u7248 \u5b9a\u91cfPCR\uff08qPCR ; quantitative PCR\uff09\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR \u00a0<\/a>\u00a0\u00a0kanako<\/p>\n

\u7ffb\u8bd1\u6295\u7a3f \u70b8\u9e21 \u6821\u5bf9 HaoHu<\/strong><\/p>\n

\u5b9e\u65f6\u8367\u5149\u5b9a\u91cfPCR \u5229\u7528\u805a\u5408\u9176\u94fe\u5f0f\u53cd\u5e94\uff08PCR\uff09<\/a>\u6765\u5b9a\u91cf\u5206\u6790\u5f85\u6d4b\u6837\u54c1\u4e2d\u7279\u5b9a\u7684DNA\u5e8f\u5217\u3002\u5982\u679c\u4f7f\u7528\u8be5\u65b9\u6cd5\uff0c\u5c31\u53ef\u4ee5\u8c03\u67e5\u9776\u57fa\u56e0\u7684\u8868\u8fbe\u91cf\u548c\u6837\u672c\u4e2d\u75c5\u6bd2\u57fa\u56e0\u7684\u542b\u91cf\u3002\u56e0\u4e3a\u4e00\u822c\u7684qPCR\u662f\u5b9e\u65f6\u5b9a\u91cf\u5206\u6790\u7684\uff0c\u6240\u4ee5\u5f88\u591a\u65f6\u5019\uff0cqPCR\u53c8\u79f0\u4f5c\u5b9e\u65f6\u8367\u5149\u5b9a\u91cfPCR\u3002\u5e38\u7528\u7684\u68c0\u6d4b\u65b9\u6cd5\u6709\u7eff\u8272\u73af\u4fdd\u6cd5(SYBR Green)\u548cTaqMan\u63a2\u9488\u6cd5(TaqMan Probe)\u4e24\u79cd\u3002<\/p>\n

\u90a3\u4e48\u5b9e\u65f6\u8367\u5149\u5b9a\u91cfPCR\u548c\u666e\u901a\u7684PCR\u6709\u4ec0\u4e48\u533a\u522b\u5462\uff1f\u666e\u901a\u7684PCR\u5229\u7528DNA\u6269\u589e\u53cd\u5e94\u540e<\/strong>DNA\u5355\u94fe\u7684\u91cd\u7ec4\uff0c\u53ea\u4ee5\u91cd\u7ec4\u540e\u7684DNA\u53cc\u94fe\u4f5c\u4e3a\u5bf9\u8c61\uff1b\u4f46\u5b9e\u65f6\u8367\u5149\u5b9a\u91cfPCR\u5219\u7740\u91cd\u4e8eDNA\u6269\u589e\u53cd\u5e94\u4e2d<\/strong>\uff0c\u6545\u5f97\u540d\u201c\u5b9e\u65f6\u8367\u5149\u5b9a\u91cfPCR\u201d\u3002qPCR\u8fd8\u6709\u4e00\u79cd\u4e0d\u662f\u5b9e\u65f6\u6d4b\u91cf\u7684\u65b9\u6cd5\uff0c\u5373\u901a\u8fc7\u7535\u6cf3\u5206\u79bb\u6269\u589e\u540e\u7684DNA\u6837\u672c\u8fdb\u884c\u5b9a\u91cf\u5206\u6790\u3002<\/p>\n

\uff08\u8bf7\u6ce8\u610f\uff0c\u751f\u7269\u76f8\u5173\u9886\u57df\u7ecf\u5e38\u770b\u5230\u201cRT-PCR\u201d\u5b57\u6837\uff0c\u4f46\u8fd9\u5e76\u4e0d\u662f\u201c\u5b9e\u65f6\u8367\u5149\u5b9a\u91cfPCR\u201d\u7684\u610f\u601d\uff0c\u5b83\u5176\u5b9e\u662f\u201c\u53cd\u8f6c\u5f55\u201d\uff08reverse transcription PCR\uff09\u7684\u610f\u601d\u3002\u8fd9\u4e00\u70b9\u8bf7\u5404\u4f4d\u8981\u7279\u522b\u6ce8\u610f\u3002\uff09<\/p>\n

1. qPCR<\/strong>\uff1a<\/strong>SYBR Green<\/strong>\u6cd5\uff08\u63ba\u5165\u6cd5\uff09<\/strong><\/h4>\n

\u63ba\u5165\u6cd5\u662f\u901a\u8fc7\u5411DNA\u53cc\u94fe\u4e2d\u63ba\u5165\u8367\u5149\u5206\u5b50\u6765\u5b9a\u91cf\u5206\u6790DNA\u5206\u5b50\u7684\u590d\u5236\u91cf\u7684\u3002SYBR Green\u662f\u5e38\u7528\u7684\u4e00\u79cd\u8367\u5149\u5206\u5b50\u3002<\/p>\n

\"\"<\/a><\/p>\n

SYBR Green I\u7684\u5316\u5b66\u6784\u9020<\/p>\n

\u4e0b\u9762\u662fSYBR Green\u6cd5\u7684\u8fc7\u7a0b\u3002<\/p>\n

    \n
  1. \u5411PCR\u53cd\u5e94\u6eb6\u6db2\u4e2d\u52a0\u5165\u80fd\u63ba\u5165DNA\u53cc\u94fe\u7684\u8367\u5149\u5206\u5b50\u3002<\/li>\n
  2. DNA\u5f00\u59cb\u6269\u589e\uff0c\u8367\u5149\u5206\u5b50\u63ba\u5165DNA\u5206\u5b50\u4e2d\u5e76\u53d1\u5c04\u8367\u5149\u4fe1\u53f7\u3002<\/li>\n
  3. \u6839\u636e\u53d1\u5c04\u7684\u8367\u5149\u4fe1\u53f7\u6765\u5b9a\u91cf\u5206\u6790DNA\u7684\u6269\u589e\u91cf\u3002<\/li>\n<\/ol>\n

    \"\"<\/a><\/p>\n

    \u4f46\u8fd9\u4e2a\u65b9\u6cd5\u6709\u4e2a\u7f3a\u70b9\uff1a\u975e\u68c0\u6d4b\u5bf9\u8c61\u7684DNA\u5355\u94fe\u4e5f\u4f1a\u975e\u7279\u5f02\u6027\u5730\u6269\u589e\uff0c\u8367\u5149\u5206\u5b50\u4e5f\u4f1a\u63ba\u5165\u5176\u4e2d\u5e76\u53d1\u51fa\u8367\u5149\u4fe1\u53f7\u3002\u4f46\u662f\u6211\u4eec\u4e0b\u9762\u4ecb\u7ecd\u7684TaqMan\u63a2\u9488\u6cd5\u5177\u6709\u53ea\u5728\u76ee\u7684DNA\u4e2d\u53d1\u5c04\u8367\u5149\u4fe1\u53f7\u7684\u4f18\u70b9\u3002<\/p>\n

    2. qPCR: TaqMan Probe<\/strong>\u6cd5(<\/strong>\u6c34\u89e3\u63a2\u9488\u6cd5\uff09<\/strong><\/h4>\n

    TaqMan Probe\u6cd5\u662f\u5229\u7528FRET\uff08\u8367\u5149\u5171\u632f\u80fd\u91cf\u8f6c\u79fb\uff09<\/a>\u6765\u5b9a\u91cf\u6d4b\u5b9aDNA\u7684\u6269\u589e\u91cf\u3002TaqMan<\/strong>\u63a2\u9488\u548c<\/strong>Taq DNA<\/strong>\u805a\u5408\u9176<\/strong>\u662f\u4e24\u4e2a\u91cd\u8981\u7684\u89d2\u8272\u3002<\/p>\n