{"id":3585,"date":"2015-01-19T23:57:49","date_gmt":"2015-01-19T15:57:49","guid":{"rendered":"\/\/www.gsbet888.com\/?p=3585"},"modified":"2019-08-02T18:55:56","modified_gmt":"2019-08-02T10:55:56","slug":"post-3585","status":"publish","type":"post","link":"\/\/www.gsbet888.com\/chemists\/2015\/01\/post-3585.html","title":{"rendered":"\u4e0b\u6751 \u8129 Osamu Shimomura"},"content":{"rendered":"<p>\u4e0b\u6751 \u8129(Shimorura Osamu\u30011928\u5e748\u670827\u65e5-) \u5b9a\u5c45\u7f8e\u56fd\u7684\u751f\u7269\u5b66\u5bb6\u3001\u6709\u673a\u5316\u5b66\u5bb6\u3002\u7f8e\u6ce2\u58eb\u987f\u5927\u5b66\u540d\u8a89\u6559\u6388\u3001<b>\u4f0d\u5179\u970d\u5c14\u6d77\u6d0b\u7814\u7a76\u6240<\/b>\u540d\u8a89\u6559\u6388\u3002<\/p>\n<p>&nbsp;<\/p>\n<h4>\u7ecf\u5386<\/h4>\n<p>1928\u5e74 \u751f\u4e8e\u4eac\u90fd\u7684\u798f\u77e5\u5c71\u3002<\/p>\n<p>1951 \u957f\u5d0e\u533b\u79d1\u5927\u5b66\u4ed8\u5c5e\u836f\u5b66\u4e13\u95e8\u90e8 \u6bd5\u4e1a<br \/>\n1958 \u957f\u5d0e\u5927\u5b66\u836f\u5b66\u90e8 \u52a9\u624b<br \/>\n1960 \u540d\u53e4\u5c4b\u5927\u5b66\u7406\u5b66\u90e8 \u535a\u58eb(<a class=\"ext-link ext-icon-2\" title=\"\" href=\"http:\/\/www.chem-station.com\/chemist-db\/archives\/2009\/02\/-yoshimasa-hirata.php\" rel=\"external\" data-wpel-target=\"_blank\">\u5e73\u7530\u4e49\u6b63<\/a>\u6559\u6388)<br \/>\n1960 \u666e\u6797\u65af\u987f\u5927\u5b66 \u7814\u7a76\u5458 (Frank Johnson\u6559\u6388)<br \/>\n1963 \u540d\u53e4\u5c4b\u5927\u5b66 \u52a9\u6559\u6388<br \/>\n1965 \u666e\u6797\u65af\u987f\u5927\u5b66 \u9ad8\u7ea7\u7814\u7a76\u5458<br \/>\n1981 \u6ce2\u58eb\u987f\u5927\u5b66 \u6559\u6388<br \/>\n1982 <b>\u4f0d\u5179\u970d\u5c14\u6d77\u6d0b\u7814\u7a76\u6240<\/b>\u00a0\u9ad8\u7ea7\u7814\u7a76\u5458<br \/>\n2001 <b>\u4f0d\u5179\u970d\u5c14\u6d77\u6d0b\u7814\u7a76\u6240<\/b>\u00a0\u9000\u804c<\/p>\n<p>&nbsp;<\/p>\n<h4>\u83b7\u5956\u7ecf\u5386<\/h4>\n<p>2004 Pearse Prize<br \/>\n2005 Emile Chamot Award<br \/>\n2006 \u671d\u65e5\u8cde<br \/>\n2008\u00a0<span class=\"wp_keywordlink\">\u8bfa\u8d1d\u5c14\u5316\u5b66\u8cde<\/span><br \/>\n2008 \u6587\u5316\u52cb\u7ae0\u30fb\u6587\u5316\u529f\u52b3\u8005<\/p>\n<p>&nbsp;<\/p>\n<h4>\u7814\u7a76\u6982\u8981<\/h4>\n<p><b><span class=\"wp_keywordlink_affiliate\">\u7eff\u8272\u8367\u5149\u86cb\u767d\u8d28 \u6c34\u6bcd\u7d20aequorin\u7684\u5206\u79bb\u00a0<\/span><\/b><\/p>\n<p>(\u4e16\u754c\u4e0a\u9996\u6b21\u4ece\u7ef4\u591a\u5229\u4e9a\u591a\u7ba1\u53d1\u5149\u6c34\u6bcd\u4e2d\u5206\u7406\u51fa\u80fd\u53d1\u51fa\u7eff\u8272\u8367\u5149\u7684\u86cb\u767d\u8d28<b><span class=\"wp_keywordlink_affiliate\"><a class=\"ext-link ext-icon-2\" title=\"View all posts in GFP\" href=\"http:\/\/www.chem-station.com\/tag\/gfp\" rel=\"external\" data-wpel-target=\"_blank\">GFP<\/a>\u00a0\u6c34\u6bcd\u7d20<\/span><\/b>[1]\u3002<\/p>\n<p>GFP\u7684\u53d1\u5149\u4e0d\u9700\u8981\u7279\u522b\u7684\u57fa\u8d28\u3001\u9176\uff0c\u5355\u4f53\u5c31\u80fd\u53d1\u6325\u673a\u80fd\u3002\u6839\u636e\u8fd9\u4e00\u6027\u8d28\uff0cGFP\u5bf9\u751f\u7269\u6d3b\u6027\u7269\u8d28\u7684\u5f71\u54cd\u8f83\u5c0f\uff0c\u53ef\u4ee5\u4f5c\u4e3a\u975e\u5e38\u9002\u7528\u7684\u751f\u7269\u5de5\u5177\u3002GFP\u5728\u751f\u7269\u4f53\u5185\u4ea7\u751f\u4fe1\u53f7\u7684\u73b0\u8c61\u3001\u88ab\u7528\u4f5c\u751f\u7269\u9ad8\u5206\u5b50\u7684\u5b58\u5728\u4f4d\u7f6e\u7b49\u53ef\u89c6\u5316\u5de5\u5177\u3002\u8fd0\u7528\u57fa\u56e0\u5de5\u7a0b\u7684\u65b9\u6cd5\u5c06\u62a5\u544a\u57fa\u56e0\u5bfc\u5165\uff0c\u5e76\u80fd\u591f\u8868\u8fbe\u7eff\u8272\u8367\u5149\u86cb\u767d\u5728\u6240\u9700\u7684\u70b9\u3002<\/p>\n<p style=\"text-align: center\"><a href=\"https:\/\/assets.www.gsbet888.com\/uploads\/2015\/01\/GFP.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-3624\" src=\"https:\/\/assets.www.gsbet888.com\/uploads\/2015\/01\/GFP.jpg\" alt=\"GFP\" width=\"192\" height=\"192\" srcset=\"https:\/\/assets.www.gsbet888.com\/uploads\/2015\/01\/GFP.jpg 192w, https:\/\/assets.www.gsbet888.com\/uploads\/2015\/01\/GFP-150x150.jpg 150w, https:\/\/assets.www.gsbet888.com\/uploads\/2015\/01\/GFP-100x100.jpg 100w, https:\/\/assets.www.gsbet888.com\/uploads\/2015\/01\/GFP-110x110.jpg 110w\" sizes=\"(max-width: 192px) 100vw, 192px\" \/><\/a><br \/>\n(\u753b\u50cf\uff1a<a class=\"ext-link ext-icon-2\" title=\"\" href=\"http:\/\/www.tsienlab.ucsd.edu\/\" rel=\"external\" data-wpel-target=\"_blank\">Tsien Laboratory<\/a>)<\/p>\n<p>\u4e0b\u6751\u6559\u6388\u6839\u636e\u4e00\u7cfb\u5217\u7684\u7814\u7a76\uff0c\u63d0\u51fa\u4e86GFP\u53d1\u8272\u56e2\u7684\u5206\u5b50\u6784\u9020\u6a21\u578b\u53ca\u53d1\u5149\u539f\u7406\uff0c\u90fd\u662f\u5728\u5316\u5b66\u7684\u57fa\u7840\u4e0a\u5efa\u7acb\u7684\u3002\u4e5f\u56e0\u8be5\u8d21\u732e\u4e8e2008\u5e74\u83b7\u5f97\u8bfa\u8d1d\u5c14\u5316\u5b66\u5956\u3002<\/p>\n<p>&nbsp;<\/p>\n<p><b>\u901a\u8fc7\u8367\u5149\u7d20\u30fb\u8367\u5149\u7d20\u9176\u89e3\u660e\u751f\u7269\u53d1\u5149\u7684\u673a\u7406<\/b><\/p>\n<p>\u901a\u8fc7\u9176\u4e3a\u5a92\u4ecb\u53d1\u751f\u5316\u5b66\u53d8\u5316\u4ea7\u751f\u5149\u7684\u7269\u8d28\u53eb\u505a\u8367\u5149\u7d20<b>(luciferin)<\/b>\u3001\u50ac\u5316\u5176\u5316\u5b66\u53cd\u5e94\u7684\u50ac\u5316\u9176\u53eb\u505a\u8367\u5149\u7d20\u9176<b>(luciferase)<\/b>\u3002\u50cfGFP\u90a3\u6837\u4e0d\u9700\u8981\u5916\u90e8\u56e0\u7d20\u800c\u53d1\u5149\u7684\u751f\u7269\u6d3b\u6027\u7269\u8d28\u771f\u7684\u975e\u5e38\u7f55\u89c1\uff0c\u751f\u7269\u53d1\u5149\u4e2d\u5927\u591a\u4ee5\u8fd9\u6837\u7684\u9176\u5316\u5b66\u53cd\u5e94\u800c\u8fdb\u884c\u3002<\/p>\n<p>\u4e0b\u6751\u5bf9\u542b\u6709\u6c34\u6bcd\u7d20\u86cb\u767d\u7b49\u7684\u8367\u5149\u7d20\u7684\u7269\u8d28\uff0c\u8154\u80a0\u7d20\u7b49\u53d1\u5149\u673a\u7406\u8fdb\u884c\u4e86\u9610\u660e\u3002<\/p>\n<p style=\"text-align: center\"><a href=\"https:\/\/assets.www.gsbet888.com\/uploads\/2015\/01\/coelenterazine_1.gif\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-3625\" src=\"https:\/\/assets.www.gsbet888.com\/uploads\/2015\/01\/coelenterazine_1.gif\" alt=\"coelenterazine_1\" width=\"539\" height=\"190\" \/><\/a><\/p>\n<p>&nbsp;<\/p>\n<h4>\u8bc4\u8bba\uff06\u5176\u4ed6<\/h4>\n<ol>\n<li style=\"text-align: left\">\u5728\u5927\u5b66\u65f6\u4ee3\u5728\u5e73\u7530\u7814\u6210\u529f\u7ed3\u6676\u5230\u6d77\u8424\u8367\u5149\u7d20\u3002\u6bd5\u4e1a\u540e\u7ee7\u7eed\u8fdb\u884c\u53d1\u5149\u7269\u8d28\u7684\u76f8\u5173\u7814\u7a76\u3002\u5728\u5f53\u65f6\u8fd9\u5e76\u4e0d\u662f\u975e\u5e38\u70ed\u95e8\u7684\u7814\u7a76\u9886\u57df\uff0c\u6709\u65f6\u8fd8\u5728\u8d44\u91d1\u7ecf\u8d39\u7d27\u5f20\u7684\u60c5\u51b5\u4e0b\u7ee7\u7eed\u7814\u7a76\u3002<\/li>\n<li>\u9577\u5d0e\u539f\u5b50\u5f39\u6295\u4e0b\u4f7f\uff0c\u4ed6\u770b\u5230\u4e86\u758f\u6563\u7684\u4eba\u7fa4\uff0c\u88ab\u642c\u8fd0\u5230\u5b66\u6821\u6821\u820d\u7684\u6b7b\u4f24\u8005\uff0c\u6df1\u6df1\u5730\u7ed9\u4ed6\u7559\u4e0b\u96be\u4ee5\u78e8\u706d\u7684\u60e8\u75db\u8bb0\u5fc6\u3002<\/li>\n<li>\u513f\u5b50<a class=\"ext-link ext-icon-2\" title=\"\" href=\"http:\/\/ja.wikipedia.org\/wiki\/%E4%B8%8B%E6%9D%91%E5%8A%AA\" rel=\"external\" data-wpel-target=\"_blank\">\u4e0b\u6751\u52aa<\/a>\u662f\u7535\u8111\u79d1\u5b66\u5bb6\u30fb\u7269\u7406\u5b66\u5bb6\u3002\u901a\u8fc7\u4f7f\u7528<a title=\"\u871c\u7f50 (\u8ba1\u7b97\u673a\u79d1\u5b66)\" href=\"http:\/\/zh.wikipedia.org\/wiki\/%E8%9C%9C%E7%BD%90_(%E9%9B%BB%E8%85%A6%E7%A7%91%E5%AD%B8)\">\u871c\u7f50\u6280\u672f<\/a>\u8bbe\u7acb\u4e86\u201c\u871c\u7f50\u201d \u8bf1\u9975\uff0c\u8ba9\u4e16\u754c\u5934\u53f7\u9ed1\u5ba2 <b>\u51ef\u6587\u00b7\u7c73\u7279\u5c3c\u514b<\/b>\u6700\u7ec8\u843d\u7f51\u3002\u4ed6\u7684\u534f\u4f5c\u902e\u6355\u5728\u4e16\u754c\u4e0a\u5e7f\u4e3a\u4eba\u77e5\u3002<\/li>\n<li>1960\u5e74\u6e21\u7f8e\uff0c\u4e4b\u540e\u4e00\u76f4\u5728\u7f8e\u56fd\u5b9a\u5c45\uff0c\u5374\u4e00\u76f4\u4fdd\u6709\u65e5\u672c\u56fd\u7c4d\u3002<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<h4>\u76f8\u5173\u8bba\u6587<\/h4>\n[1] Shimomura, O.; Johnson, F. H.; Saiga, Y.\u00a0<i>J. Cell. Comp. Physiol.<\/i>\u00a0<b>1962<\/b>,\u00a0<i>59<\/i>, 223.<br \/>\n[2] Shimomura, O.; Johnson, F. H.\u00a0\u00a0<i>Proc. Natl. Acad. Sci. USA<\/i>\u00a0<b>1975<\/b>,\u00a0<i>72<\/i>, 1546.<\/p>\n<p>&nbsp;<\/p>\n<p><strong>\u672c\u6587\u7248\u6743\u5c5e\u4e8e\u00a0<a href=\"\/\/www.gsbet888.com\/\">Chem-Station\u5316\u5b66\u7a7a\u95f4<\/a>\uff0c \u6b22\u8fce\u70b9\u51fb\u6309\u94ae\u5206\u4eab\uff0c\u672a\u7ecf\u8bb8\u53ef\uff0c\u8c22\u7edd\u8f6c\u8f7d\uff01<\/strong> <\/p>\n","protected":false},"excerpt":{"rendered":"\u4e0b\u6751 \u8129(Shimorura Osamu\u30011928\u5e748\u670827\u65e5-) \u5b9a\u5c45\u7f8e\u56fd\u7684\u751f\u7269\u5b66\u5bb6\u3001\u6709\u673a\u5316\u5b66\u5bb6\u3002\u7f8e\u6ce2\u58eb\u987f\u5927\u5b66\u540d\u8a89\u6559\u6388\u3001\u4f0d\u5179\u970d\u5c14\u6d77\u6d0b\u7814\u7a76\u6240\u540d\u8a89\u6559\u6388\u3002 &nbsp; \u7ecf\u5386 1928\u5e74 \u751f\u4e8e\u4eac\u90fd\u7684\u798f\u77e5\u5c71\u3002 1951 \u957f\u5d0e\u533b\u79d1\u5927\u5b66\u4ed8\u5c5e\u836f\u5b66\u4e13\u95e8\u90e8 \u6bd5\u4e1a 1958 \u957f\u5d0e\u5927\u5b66\u836f\u5b66\u90e8 \u52a9\u624b 1960 \u540d\u53e4\u5c4b\u5927\u5b66\u7406\u5b66\u90e8 \u535a\u58eb(\u5e73\u7530\u4e49\u6b63\u6559\u6388) 1960 \u666e\u6797\u65af\u987f\u5927\u5b66 \u7814\u7a76\u5458 (Frank Johnson\u6559\u6388) 1963 \u540d\u53e4\u5c4b\u5927\u5b66 \u52a9\u6559\u6388 1965 \u666e\u6797\u65af\u987f\u5927\u5b66 \u9ad8\u7ea7\u7814\u7a76\u5458 1981 \u6ce2\u58eb\u987f\u5927\u5b66 \u6559\u6388 1982 \u4f0d\u5179\u970d\u5c14\u6d77\u6d0b\u7814\u7a76\u6240\u00a0\u9ad8\u7ea7\u7814\u7a76\u5458 2001 \u4f0d\u5179\u970d\u5c14\u6d77\u6d0b\u7814\u7a76\u6240\u00a0\u9000\u804c &nbsp; \u83b7\u5956\u7ecf\u5386 2004 Pearse Prize 2005 Emile Chamot Award 2006 \u671d\u65e5\u8cde 2008\u00a0\u8bfa\u8d1d\u5c14\u5316\u5b66\u8cde 2008 \u6587\u5316\u52cb\u7ae0\u30fb\u6587\u5316\u529f\u52b3\u8005 &nbsp; \u7814\u7a76\u6982\u8981 \u7eff\u8272\u8367\u5149\u86cb\u767d\u8d28 \u6c34\u6bcd\u7d20aequorin\u7684\u5206\u79bb\u00a0 (\u4e16\u754c\u4e0a\u9996\u6b21\u4ece\u7ef4\u591a\u5229\u4e9a\u591a\u7ba1\u53d1\u5149\u6c34\u6bcd\u4e2d\u5206\u7406\u51fa\u80fd\u53d1\u51fa\u7eff\u8272\u8367\u5149\u7684\u86cb\u767d\u8d28GFP\u00a0\u6c34\u6bcd\u7d20[1]\u3002 GFP\u7684\u53d1\u5149\u4e0d\u9700\u8981\u7279\u522b\u7684\u57fa\u8d28\u3001\u9176\uff0c\u5355\u4f53\u5c31\u80fd\u53d1\u6325\u673a\u80fd\u3002\u6839\u636e\u8fd9\u4e00\u6027\u8d28\uff0cGFP\u5bf9\u751f\u7269\u6d3b\u6027\u7269\u8d28\u7684\u5f71\u54cd\u8f83\u5c0f\uff0c\u53ef\u4ee5\u4f5c\u4e3a\u975e\u5e38\u9002\u7528\u7684\u751f\u7269\u5de5\u5177\u3002GFP\u5728\u751f\u7269\u4f53\u5185\u4ea7\u751f\u4fe1\u53f7\u7684\u73b0\u8c61\u3001\u88ab\u7528\u4f5c\u751f\u7269\u9ad8\u5206\u5b50\u7684\u5b58\u5728\u4f4d\u7f6e\u7b49\u53ef\u89c6\u5316\u5de5\u5177\u3002\u8fd0\u7528\u57fa\u56e0\u5de5\u7a0b\u7684\u65b9\u6cd5\u5c06\u62a5\u544a\u57fa\u56e0\u5bfc\u5165\uff0c\u5e76\u80fd\u591f\u8868\u8fbe\u7eff\u8272\u8367\u5149\u86cb\u767d\u5728\u6240\u9700\u7684\u70b9\u3002 (\u753b\u50cf\uff1aTsien Laboratory) \u4e0b\u6751\u6559\u6388\u6839\u636e\u4e00\u7cfb\u5217\u7684\u7814\u7a76\uff0c\u63d0\u51fa\u4e86GFP\u53d1\u8272\u56e2\u7684\u5206\u5b50\u6784\u9020\u6a21\u578b\u53ca\u53d1\u5149\u539f\u7406\uff0c\u90fd\u662f\u5728\u5316\u5b66\u7684\u57fa\u7840\u4e0a\u5efa\u7acb\u7684\u3002\u4e5f\u56e0\u8be5\u8d21\u732e\u4e8e2008\u5e74\u83b7\u5f97\u8bfa\u8d1d\u5c14\u5316\u5b66\u5956\u3002 &nbsp; 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\u76f8\u5173\u8bba\u6587 [1] Shimomura, O.; Johnson, F. H.; Saiga, Y.\u00a0J. Cell. Comp. Physiol.\u00a01962,\u00a059, 223. [2] Shimomura, O.; Johnson, F. H.\u00a0\u00a0Proc. Natl. Acad. Sci. 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