{"id":42294,"date":"2022-07-08T08:00:46","date_gmt":"2022-07-08T00:00:46","guid":{"rendered":"\/\/www.gsbet888.com\/?p=42294"},"modified":"2022-07-31T16:36:51","modified_gmt":"2022-07-31T08:36:51","slug":"kaiser-test","status":"publish","type":"post","link":"\/\/www.gsbet888.com\/%e5%8c%96%e5%ad%a6%e6%9d%82%e8%ae%b0\/%e5%ae%9e%e9%aa%8c%e6%8a%80%e5%b7%a7\/2022\/07\/kaiser-test.html","title":{"rendered":"Kaiser Test"},"content":{"rendered":"<h4 style=\"font-weight: 400;\"><strong>\u6982\u8981<\/strong><\/h4>\n<p style=\"font-weight: 400;\">\u5982\u679c\u4f7f\u7528\u56fa\u76f8\u5408\u6210\u6765\u5408\u6210\u591a\u80bd\u7684\u7f29\u5408\u53cd\u5e94\u6ca1\u6709\u5b8c\u5168\u8fdb\u884c\uff0c\u5c31\u4f1a\u5bfc\u81f4\u6700\u540e\u5408\u6210\u7684\u591a\u80bd\u662f\u5404\u79cd\u94fe\u957f\u7684\u591a\u80bd\u7684\u6df7\u5408\u7269\u3002\u8fd9\u4f1a\u7ed9\u591a\u80bd\u7684\u63d0\u7eaf\u5e26\u6765\u5f88\u9ad8\u7684\u96be\u5ea6\u3002<\/p>\n<p style=\"font-weight: 400;\">\u4e3a\u4e86\u786e\u4fdd\u8fd9\u4e2a\u53cd\u5e94\u7684\u4ea7\u7387\u8fbe\u5230100%\uff0c\u6709\u4e00\u79cd\u7b80\u4fbf\u7684\u5206\u6790\u65b9\u6cd5\u53ef\u4ee5\u7528\u4e8e\u68c0\u6d4b\u591a\u80bd\u56fa\u76f8\u5408\u6210\u7684\u7f29\u5408\u60c5\u51b5\uff0c\u8fd9\u4e2a\u65b9\u6cd5\u5c31\u662fKaiser Test\u3002\u5c31\u50cf\u56fa\u76f8\u5408\u6210\u4e2d\u7684TLC\u4e00\u6837\uff0c\u5728\u5b9e\u9a8c\u65f6\u53ea\u8981\u4ed4\u7ec6\u64cd\u4f5c\uff0c\u5c31\u5bb9\u6613\u83b7\u5f97\u9ad8\u6536\u7387\u3001\u9ad8\u7eaf\u5ea6\u7684\u591a\u80bd\u3002\uff08\u5f00\u5934\u7684\u56fe\u7247\u5f15\u7528\u81ea<a href=\"https:\/\/www.sciencedirect.com\/science\/article\/abs\/pii\/S0109564120300385?via=ihub\">\u8fd9\u91cc<\/a>\uff09<\/p>\n<h4>\u539f\u7406<\/h4>\n<p style=\"font-weight: 400;\">Kaiser Test\u7684\u539f\u7406\u662f\u56fa\u76f8\u4e0a\u7684\u831a\u4e09\u916e\u663e\u8272\u53cd\u5e94\uff0c\u8be5\u5206\u6790\u65b9\u6cd5\u76f8\u5f53\u4e8e\u68c0\u6d4b\u51fa\u672b\u7aef\u6c28\u57fa\u3002\u831a\u4e09\u916e\u4e0e\u6c28\u57fa\u53cd\u5e94\u4ea7\u751f\u4e00\u79cdRuhemann\u2019s purple\u7684\u8272\u7d20\u3002\u6d4b\u8bd5\u8fc7\u7a0b\u4e2d\u52a0\u5165\u82ef\u915a\u662f\u4e3a\u4e86\u8c03\u8282PH\u503c\uff0cKCN\u7684\u4f5c\u7528\u662f\u8fd8\u539f\u5242\uff0c\u662f\u4e3a\u4e86\u9632\u6b62\u4e2d\u95f4\u4f53\u88ab\u7a7a\u6c14\u6c27\u5316<sup>[3]<\/sup>\u3002<\/p>\n<p><a href=\"https:\/\/assets.www.gsbet888.com\/uploads\/2022\/07\/455e09ae579b2b1f5f958b1dd37759e8-3.png\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-42291 size-large\" src=\"https:\/\/assets.www.gsbet888.com\/uploads\/2022\/07\/455e09ae579b2b1f5f958b1dd37759e8-3-680x468.png\" alt=\"\" width=\"680\" height=\"468\" srcset=\"https:\/\/assets.www.gsbet888.com\/uploads\/2022\/07\/455e09ae579b2b1f5f958b1dd37759e8-3-680x468.png 680w, https:\/\/assets.www.gsbet888.com\/uploads\/2022\/07\/455e09ae579b2b1f5f958b1dd37759e8-3-300x207.png 300w, https:\/\/assets.www.gsbet888.com\/uploads\/2022\/07\/455e09ae579b2b1f5f958b1dd37759e8-3-768x529.png 768w, https:\/\/assets.www.gsbet888.com\/uploads\/2022\/07\/455e09ae579b2b1f5f958b1dd37759e8-3.png 816w\" sizes=\"(max-width: 680px) 100vw, 680px\" \/><\/a><\/p>\n<h5>\u8bd5\u9a8c\u987a\u5e8f<sup>[1]<\/sup><\/h5>\n<p style=\"font-weight: 400;\">\u8bd5\u5242\u4e0e\u6750\u6599<\/p>\n<p style=\"font-weight: 400;\">\u8bd5\u5242\u7ec4\u867d\u7136\u53ef\u4ee5\u8d2d\u4e70\u6210\u54c1\uff0c\u4f46\u4e5f\u53ef\u4ee5\u6839\u636e\u4e2a\u4eba\u9700\u8981\u4ece\u5ec9\u4ef7\u7684\u8bd5\u5242\u4e2d\u81ea\u5236\u3002\u6df7\u5408\u6216\u8c03\u5236\u4ee5\u4e0b\u6eb6\u6db2\u3002<\/p>\n<p style=\"font-weight: 400;\"><strong><u>\u8bd5\u6db2<\/u><\/strong><strong><u>1<\/u><\/strong><\/p>\n<p style=\"font-weight: 400;\">\u831a\u4e09\u916e\uff1a500 mg<\/p>\n<p style=\"font-weight: 400;\">\u4e59\u9187\uff1a10 mL<\/p>\n<p style=\"font-weight: 400;\"><strong><u>\u8bd5\u6db2<\/u><\/strong><strong><u>2<\/u><\/strong><\/p>\n<p style=\"font-weight: 400;\">\u82ef\u915a\uff1a80\u514b<\/p>\n<p style=\"font-weight: 400;\">\u4e59\u9187\uff1a20 mL<\/p>\n<p style=\"font-weight: 400;\"><strong><u>\u8bd5\u6db2<\/u><\/strong><strong><u>3<\/u><\/strong><\/p>\n<p style=\"font-weight: 400;\">1mM KCN\u6c34\u6eb6\u6db2\uff1a2 mL<\/p>\n<p style=\"font-weight: 400;\">\u5421\u5576\uff1a100 mL<\/p>\n<p style=\"font-weight: 400;\"><strong>\u6b65\u9aa4<\/strong><\/p>\n<ol>\n<li style=\"font-weight: 400;\">\u5c06\u5c11\u91cf\u56fa\u76f8\u5408\u6210\u6811\u8102\u52a0\u5165\u5fae\u7ba1\u4e2d\uff0c\u7528MeOH\u6e05\u6d173\u6b21\uff08\u53bb\u9664\u4f53\u7cfb\u91cc\u7684DMF\uff09\u3002<\/li>\n<\/ol>\n<p style=\"font-weight: 400;\">2.\u5411\u5fae\u7ba1\u4e2d\u52a0\u5165\u8bd5\u6db21~3\u5404\u6ef41\u6ef4\uff0c100\u2103\u4e0b\u52a0\u70ed1\u5206\u949f\u3002<\/p>\n<ol start=\"3\">\n<li style=\"font-weight: 400;\">\u5982\u679c\u5fae\u7ba1\u5185\u7684\u6eb6\u6db2\u5448\u6df1\u84dd\u8272\u5230\u7d2b\u8272\uff0c\u5219\u8868\u793a\u5b58\u5728\u672a\u88ab\u53cd\u5e94\u6389\u7684\u672b\u7aef\u6c28\u57fa\u3002\u65e0\u8272~\u9ec4\u8272\u7684\u60c5\u51b5\uff0c\u8868\u793a\u7f29\u5408\u53cd\u5e94\u5df2\u7ecf\u5b8c\u6210\u3002<\/li>\n<\/ol>\n<p><a href=\"https:\/\/assets.www.gsbet888.com\/uploads\/2022\/07\/2-3.png\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-42292 size-large\" src=\"https:\/\/assets.www.gsbet888.com\/uploads\/2022\/07\/2-3-680x391.png\" alt=\"\" width=\"680\" height=\"391\" srcset=\"https:\/\/assets.www.gsbet888.com\/uploads\/2022\/07\/2-3-680x391.png 680w, https:\/\/assets.www.gsbet888.com\/uploads\/2022\/07\/2-3-300x173.png 300w, https:\/\/assets.www.gsbet888.com\/uploads\/2022\/07\/2-3-768x442.png 768w, https:\/\/assets.www.gsbet888.com\/uploads\/2022\/07\/2-3.png 793w\" sizes=\"(max-width: 680px) 100vw, 680px\" \/><\/a><\/p>\n<p style=\"font-weight: 400;\">\u5b58\u5728\u672a\u53cd\u5e94\u7684\u672b\u7aef\u6c28\u57fa\u65f6\uff0c\u7528\u540c\u6837\u7684\u6761\u4ef6\u518d\u6b21\u7f29\u5408\u6216\u53d8\u66f4\u7f29\u5408\u6761\u4ef6\u6216\u8fdb\u884c\u4e59\u9170\u5316\u7b49\u5904\u7406\u3002<\/p>\n<p style=\"font-weight: 400;\">\u56db\u6c2f\u5bf9\u82ef\u918c\u8bd5\u9a8c<strong><sup>[4]<\/sup><\/strong><\/p>\n<p style=\"font-weight: 400;\">Kaiser Test\u539f\u7406\u4e0a\u662f\u4e0d\u9002\u7528\u4e8e\u68c0\u6d4b\u51fa\u672b\u7aef\u6c28\u57fa\u662f\u4ef2\u80fa\u7684\u6c28\u57fa\u9178\u7684\uff08\u5982\u812f\u6c28\u9178\u548cN-\u7532\u57fa\u6c28\u57fa\u9178\uff09\u3002\u8981\u5148\u68c0\u6d4b\u51fa\u672b\u7aef\u6c28\u57fa\u662f\u975e\u4e00\u7ea7\u80fa\u7684\u6c28\u57fa\u9178\uff0c\u8981\u7528\u5230\u56db\u6c2f\u5bf9\u82ef\u918c\u3002<\/p>\n<p style=\"font-weight: 400;\"><strong>\u8bd5\u5242\u4e0e\u539f\u6599<\/strong><\/p>\n<p style=\"font-weight: 400;\"><strong><u>\u8bd5\u6db2<\/u><\/strong><strong><u>1<\/u><\/strong><\/p>\n<p style=\"font-weight: 400;\">\u4e59\u919b\uff1a1 mL<\/p>\n<p style=\"font-weight: 400;\">DMF\uff1a49 mL<\/p>\n<p style=\"font-weight: 400;\"><strong><u>\u8bd5\u6db2<\/u><\/strong><strong><u>2<\/u><\/strong><\/p>\n<p style=\"font-weight: 400;\">\u56db\u6c2f\u5bf9\u82ef\u918c\uff1a1g<\/p>\n<p style=\"font-weight: 400;\">DMF\uff1a49 mL<\/p>\n<p style=\"font-weight: 400;\"><strong>\u6b65\u9aa4<\/strong><\/p>\n<ol>\n<li style=\"font-weight: 400;\">\u5c06\u5c11\u91cf\u56fa\u76f8\u5408\u6210\u6811\u8102\u52a0\u5165\u5fae\u7ba1\u4e2d\uff0c\u7528MeOH\u6e05\u6d173\u6b21\uff08\u53bb\u9664\u4f53\u7cfb\u91cc\u7684DMF\uff09\u3002<\/li>\n<li style=\"font-weight: 400;\">\u5411\u5fae\u7ba1\u91cc\u6ef4\u52a0\u8bd5\u6db21\u548c2\u5404\u4e00\u6ef4\uff0c\u5ba4\u6e29\u4e0b\u653e\u7f6e5\u5206\u949f\u3002<\/li>\n<li style=\"font-weight: 400;\">\u5982\u679c\u6df7\u5408\u6eb6\u6db2\u5448\u73b0\u6df1\u84dd\u8272\u81f3\u7eff\u8272\u8bf4\u660e\u8fd8\u6709\u672a\u53cd\u5e94\u7684\u672b\u7aef\u6c28\u57fa\u3002\u5982\u679c\u6df7\u5408\u6eb6\u6db2\u5448\u73b0\u51fa\u65e0\u8272\u81f3\u9ec4\u8272\uff0c\u8bf4\u660e\u7f29\u5408\u53cd\u5e94\u5df2\u7ecf\u5168\u90e8\u8fdb\u884c\u5b8c\u6bd5\u3002<\/li>\n<\/ol>\n<p style=\"font-weight: 400;\">NHPI\u8bd5\u9a8c<strong><sup>[5]<\/sup><\/strong><\/p>\n<p style=\"font-weight: 400;\">\u8fd9\u662f\u4e00\u79cd\u65b0\u7684\u68c0\u6d4b\u65b9\u6848\uff0c\u53ef\u4ee5\u53ef\u9006\u5730\u5bf9\u6811\u8102\u8fdb\u884c\u67d3\u8272\u3002NHPI\u8bd5\u9a8c\u53ef\u4ee5\u68c0\u6d4b\u51fa\u542b\u6709\u4efb\u4f55\u7ea7\u6570\u6c28\u57fa\u7684\u6c28\u57fa\u9178\uff0c\u4e14\u6c28\u57fa\u9178\u79cd\u7c7b\u4e0d\u53d7\u9650\u5236\u3002\u800c\u4e14\u4e0d\u9700\u8981\u62c5\u5fc3\u7528\u5230Kaiser test\u65f6\u7528\u5230\u7684\u5267\u6bd2\u7269\u6c30\u5316\u94be\uff08KCN\uff09\u5e26\u6765\u7684\u7684\u5371\u5bb3\uff0c\u5e76\u4e14\u7528\u4e8e\u505a\u8bd5\u9a8c\u7684\u6811\u8102\u53ef\u4ee5\u56de\u6536\u5229\u7528\u7531\u6b64\u4e0d\u4f1a\u5f71\u54cd\u603b\u6536\u7387\u3002\u8be5\u65b9\u6cd5\u76f8\u6bd4Kaiser test\u65b9\u6cd5\u800c\u8a00\u6539\u5584\u4e86\u5f88\u591a\u3002<\/p>\n<p style=\"font-weight: 400;\">\u8bd5\u5242\u4e0e\u539f\u6599<\/p>\n<p style=\"font-weight: 400;\"><strong><u>\u8bd5\u6db2<\/u><\/strong><\/p>\n<ul>\n<li style=\"font-weight: 400;\">N-\u7f9f\u57fa\u90bb\u82ef\u4e8c\u7532\u9170\u4e9a\u80fa\uff08NHPI\uff09\u7684\u9971\u548cDMF\u6eb6\u6db2\uff08~2.7 M\uff09<\/li>\n<\/ul>\n<p style=\"font-weight: 400;\">\u203b\u989c\u8272\u53d8\u5316\u4e0d\u660e\u663e\u7684\u60c5\u51b5\u4e0b\uff0c\u4f7f\u7528N\uff0cN\u2019-\u4e8c\u7f9f\u57fa\u7126\u7f8e\u6797\u80fa\uff08NDHPI\uff09\u4ee3\u66ffNHPI\u53ef\u4ee5\u5f97\u5230\u6539\u5584\u3002<\/p>\n<p style=\"font-weight: 400;\">\u6b65\u9aa4<\/p>\n<ol>\n<li style=\"font-weight: 400;\">\u5c06\u5c11\u91cf\u56fa\u76f8\u5408\u6210\u6811\u8102\u52a0\u5165\u5fae\u7ba1\u4e2d\uff0c\u7528MeOH\u6e05\u6d173\u6b21\uff08\u53bb\u9664\u4f53\u7cfb\u91cc\u7684\u78b1\uff09\u3002<\/li>\n<li style=\"font-weight: 400;\">\u5c06\u8bd5\u6db2\u4e0e\u6811\u8102\u6df7\u5408\uff0c\u91cd\u590d\u6e05\u6d173\u6b21\u3002<\/li>\n<li style=\"font-weight: 400;\">\u5982\u679c\u6e05\u6d173\u6b21\u540e\u7684\u6811\u8102\u5448\u73b0\u7ea2\u8272\u81f3\u8910\u8272\u5219\u8868\u793a\u5b58\u5728\u672a\u53cd\u5e94\u7684\u672b\u7aef\u6c28\u57fa\uff0c\u5982\u679c\u6811\u8102\u5448\u73b0\u51fa\u65e0\u8272\u81f3\u9ec4\u8272\uff0c\u8868\u793a\u7f29\u5408\u53cd\u5e94\u5df2\u7ecf\u5168\u90e8\u8fdb\u884c\u5b8c\u6bd5\u3002<\/li>\n<\/ol>\n<p><a href=\"https:\/\/assets.www.gsbet888.com\/uploads\/2022\/07\/3-2.png\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-42293 size-large\" src=\"https:\/\/assets.www.gsbet888.com\/uploads\/2022\/07\/3-2-680x465.png\" alt=\"\" width=\"680\" height=\"465\" srcset=\"https:\/\/assets.www.gsbet888.com\/uploads\/2022\/07\/3-2-680x465.png 680w, https:\/\/assets.www.gsbet888.com\/uploads\/2022\/07\/3-2-300x205.png 300w, https:\/\/assets.www.gsbet888.com\/uploads\/2022\/07\/3-2-768x525.png 768w, https:\/\/assets.www.gsbet888.com\/uploads\/2022\/07\/3-2.png 887w\" sizes=\"(max-width: 680px) 100vw, 680px\" \/><\/a><\/p>\n<p style=\"font-weight: 400; text-align: center;\"><em>\uff08\u8ad6\u6587<\/em><em>[5]SI<\/em><em>\u3088\u308a\u5f15\u7528\uff09<\/em><\/p>\n<p style=\"font-weight: 400; text-align: center;\"><em>(<\/em><em>\u5f15\u7528\u81ea\u8bba\u6587<\/em><em><sup>[5]<\/sup><\/em><em>)<\/em><\/p>\n<h4 style=\"font-weight: 400;\">\u53c2\u8003\u6587\u732e<\/h4>\n<ul>\n<li style=\"font-weight: 400;\">[1] \u201dColor Test for Detection of Free Terminal Amino Groups in the Solid-Phase Synthesis of Peptides\u201d Kaiser,E. T.; Colescott, R. L.; Bossinger, C. D.; Cook, P. I.\u00a0<em>Anal. Biochem.\u00a0<\/em><strong>1970<\/strong>,\u00a0<em>34<\/em>, 595. doi: <a href=\"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/0003269770901466\">10.1016\/0003-2697(70)90146-6.<\/a><\/li>\n<li style=\"font-weight: 400;\">[2] \u201dQuantitative monitoring of solid-phase peptide synthesis by the ninhydrin reaction\u201d Sarin, V. K.; Kent, S. B. h.; Tam, J. P.; Merrifield, R. B.\u00a0<em>Anal. Biochem.<\/em>\u00a0<strong>1981<\/strong>,\u00a0<em>117<\/em>, 147. doi: <a href=\"https:\/\/doi.org\/10.1016\/0003-2697(81)90704-1\">10.1016\/0003-2697(81)90704-1<\/a>.<\/li>\n<li style=\"font-weight: 400;\">[3] \u201cA modified photometric ninhydrin method for the analysis of amino and imino acids.\u201d Troll, W.; Cannan. R. K.\u00a0<a href=\"https:\/\/www.jbc.org\/article\/S0021-9258(18)71428-2\/pdf\">J. Biol. Chem.\u00a01953,\u00a0200, 803.<\/a><\/li>\n<li style=\"font-weight: 400;\">[4] (a) \u201cA Qualitative Test for Monitoring Coupling Completeness in Solid Phase Ppetide Synthesis Using Chloranil\u201d Christensen, T.\u00a0<em>Acta Chem. Scand. B<\/em>\u00a0<strong>1979<\/strong>,\u00a0<em>33<\/em>, 763.\u00a0\u00a0<a href=\"http:\/\/actachemscand.org\/pdf\/acta_vol_33b_p0763-0766.pdf\">[PDF]<\/a><\/li>\n<li style=\"font-weight: 400;\">(b) \u201cDetection of secondary amines on solid phase\u201d Vojkovsky, T.\u00a0<em>Pept. Res.<\/em>\u00a0<strong>1995<\/strong>,\u00a0<em>8<\/em>, 236.<\/li>\n<li style=\"font-weight: 400;\">[5] \u201cStain Protocol for the Detection of N-Terminal Amino Groups during Solid-Phase Peptide Synthesis\u201d Suzuki, R.; Konno, H.\u00a0<em>Org. Lett.<\/em>\u00a0<strong>2020<\/strong>,\u00a0<em>22<\/em>, 3309. doi: <a href=\"https:\/\/pubs.acs.org\/doi\/10.1021\/acs.orglett.0c00445\">10.1021\/acs.orglett.0c00445.<\/a><\/li>\n<\/ul>\n<p>\u672c\u6587\u7248\u6743\u5c5e\u4e8e\u00a0Chem-Station\u5316\u5b66\u7a7a\u95f4\uff0c \u6b22\u8fce\u70b9\u51fb\u6309\u94ae\u5206\u4eab\uff0c\u672a\u7ecf\u8bb8\u53ef\uff0c\u8c22\u7edd\u8f6c\u8f7d<\/p>\n","protected":false},"excerpt":{"rendered":"\u6982\u8981 \u5982\u679c\u4f7f\u7528\u56fa\u76f8\u5408\u6210\u6765\u5408\u6210\u591a\u80bd\u7684\u7f29\u5408\u53cd\u5e94\u6ca1\u6709\u5b8c\u5168\u8fdb\u884c\uff0c\u5c31\u4f1a\u5bfc\u81f4\u6700\u540e\u5408\u6210\u7684\u591a\u80bd\u662f\u5404\u79cd\u94fe\u957f\u7684\u591a\u80bd\u7684\u6df7\u5408\u7269\u3002\u8fd9\u4f1a\u7ed9\u591a\u80bd\u7684\u63d0\u7eaf\u5e26\u6765\u5f88\u9ad8\u7684\u96be\u5ea6\u3002 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purple\u7684\u8272\u7d20\u3002\u6d4b\u8bd5\u8fc7\u7a0b\u4e2d\u52a0\u5165\u82ef\u915a\u662f\u4e3a\u4e86\u8c03\u8282PH\u503c\uff0cKCN\u7684\u4f5c\u7528\u662f\u8fd8\u539f\u5242\uff0c\u662f\u4e3a\u4e86\u9632\u6b62\u4e2d\u95f4\u4f53\u88ab\u7a7a\u6c14\u6c27\u5316[3]\u3002 \u8bd5\u9a8c\u987a\u5e8f[1] \u8bd5\u5242\u4e0e\u6750\u6599 \u8bd5\u5242\u7ec4\u867d\u7136\u53ef\u4ee5\u8d2d\u4e70\u6210\u54c1\uff0c\u4f46\u4e5f\u53ef\u4ee5\u6839\u636e\u4e2a\u4eba\u9700\u8981\u4ece\u5ec9\u4ef7\u7684\u8bd5\u5242\u4e2d\u81ea\u5236\u3002\u6df7\u5408\u6216\u8c03\u5236\u4ee5\u4e0b\u6eb6\u6db2\u3002 \u8bd5\u6db21 \u831a\u4e09\u916e\uff1a500 mg \u4e59\u9187\uff1a10 mL \u8bd5\u6db22 \u82ef\u915a\uff1a80\u514b \u4e59\u9187\uff1a20 mL \u8bd5\u6db23 1mM KCN\u6c34\u6eb6\u6db2\uff1a2 mL \u5421\u5576\uff1a100 mL \u6b65\u9aa4 \u5c06\u5c11\u91cf\u56fa\u76f8\u5408\u6210\u6811\u8102\u52a0\u5165\u5fae\u7ba1\u4e2d\uff0c\u7528MeOH\u6e05\u6d173\u6b21\uff08\u53bb\u9664\u4f53\u7cfb\u91cc\u7684DMF\uff09\u3002 2.\u5411\u5fae\u7ba1\u4e2d\u52a0\u5165\u8bd5\u6db21~3\u5404\u6ef41\u6ef4\uff0c100\u2103\u4e0b\u52a0\u70ed1\u5206\u949f\u3002 \u5982\u679c\u5fae\u7ba1\u5185\u7684\u6eb6\u6db2\u5448\u6df1\u84dd\u8272\u5230\u7d2b\u8272\uff0c\u5219\u8868\u793a\u5b58\u5728\u672a\u88ab\u53cd\u5e94\u6389\u7684\u672b\u7aef\u6c28\u57fa\u3002\u65e0\u8272~\u9ec4\u8272\u7684\u60c5\u51b5\uff0c\u8868\u793a\u7f29\u5408\u53cd\u5e94\u5df2\u7ecf\u5b8c\u6210\u3002 \u5b58\u5728\u672a\u53cd\u5e94\u7684\u672b\u7aef\u6c28\u57fa\u65f6\uff0c\u7528\u540c\u6837\u7684\u6761\u4ef6\u518d\u6b21\u7f29\u5408\u6216\u53d8\u66f4\u7f29\u5408\u6761\u4ef6\u6216\u8fdb\u884c\u4e59\u9170\u5316\u7b49\u5904\u7406\u3002 \u56db\u6c2f\u5bf9\u82ef\u918c\u8bd5\u9a8c[4] Kaiser Test\u539f\u7406\u4e0a\u662f\u4e0d\u9002\u7528\u4e8e\u68c0\u6d4b\u51fa\u672b\u7aef\u6c28\u57fa\u662f\u4ef2\u80fa\u7684\u6c28\u57fa\u9178\u7684\uff08\u5982\u812f\u6c28\u9178\u548cN-\u7532\u57fa\u6c28\u57fa\u9178\uff09\u3002\u8981\u5148\u68c0\u6d4b\u51fa\u672b\u7aef\u6c28\u57fa\u662f\u975e\u4e00\u7ea7\u80fa\u7684\u6c28\u57fa\u9178\uff0c\u8981\u7528\u5230\u56db\u6c2f\u5bf9\u82ef\u918c\u3002 \u8bd5\u5242\u4e0e\u539f\u6599 \u8bd5\u6db21 \u4e59\u919b\uff1a1 mL DMF\uff1a49 mL \u8bd5\u6db22 \u56db\u6c2f\u5bf9\u82ef\u918c\uff1a1g DMF\uff1a49 mL \u6b65\u9aa4 \u5c06\u5c11\u91cf\u56fa\u76f8\u5408\u6210\u6811\u8102\u52a0\u5165\u5fae\u7ba1\u4e2d\uff0c\u7528MeOH\u6e05\u6d173\u6b21\uff08\u53bb\u9664\u4f53\u7cfb\u91cc\u7684DMF\uff09\u3002 \u5411\u5fae\u7ba1\u91cc\u6ef4\u52a0\u8bd5\u6db21\u548c2\u5404\u4e00\u6ef4\uff0c\u5ba4\u6e29\u4e0b\u653e\u7f6e5\u5206\u949f\u3002 \u5982\u679c\u6df7\u5408\u6eb6\u6db2\u5448\u73b0\u6df1\u84dd\u8272\u81f3\u7eff\u8272\u8bf4\u660e\u8fd8\u6709\u672a\u53cd\u5e94\u7684\u672b\u7aef\u6c28\u57fa\u3002\u5982\u679c\u6df7\u5408\u6eb6\u6db2\u5448\u73b0\u51fa\u65e0\u8272\u81f3\u9ec4\u8272\uff0c\u8bf4\u660e\u7f29\u5408\u53cd\u5e94\u5df2\u7ecf\u5168\u90e8\u8fdb\u884c\u5b8c\u6bd5\u3002 NHPI\u8bd5\u9a8c[5] \u8fd9\u662f\u4e00\u79cd\u65b0\u7684\u68c0\u6d4b\u65b9\u6848\uff0c\u53ef\u4ee5\u53ef\u9006\u5730\u5bf9\u6811\u8102\u8fdb\u884c\u67d3\u8272\u3002NHPI\u8bd5\u9a8c\u53ef\u4ee5\u68c0\u6d4b\u51fa\u542b\u6709\u4efb\u4f55\u7ea7\u6570\u6c28\u57fa\u7684\u6c28\u57fa\u9178\uff0c\u4e14\u6c28\u57fa\u9178\u79cd\u7c7b\u4e0d\u53d7\u9650\u5236\u3002\u800c\u4e14\u4e0d\u9700\u8981\u62c5\u5fc3\u7528\u5230Kaiser test\u65f6\u7528\u5230\u7684\u5267\u6bd2\u7269\u6c30\u5316\u94be\uff08KCN\uff09\u5e26\u6765\u7684\u7684\u5371\u5bb3\uff0c\u5e76\u4e14\u7528\u4e8e\u505a\u8bd5\u9a8c\u7684\u6811\u8102\u53ef\u4ee5\u56de\u6536\u5229\u7528\u7531\u6b64\u4e0d\u4f1a\u5f71\u54cd\u603b\u6536\u7387\u3002\u8be5\u65b9\u6cd5\u76f8\u6bd4Kaiser test\u65b9\u6cd5\u800c\u8a00\u6539\u5584\u4e86\u5f88\u591a\u3002 \u8bd5\u5242\u4e0e\u539f\u6599 \u8bd5\u6db2 N-\u7f9f\u57fa\u90bb\u82ef\u4e8c\u7532\u9170\u4e9a\u80fa\uff08NHPI\uff09\u7684\u9971\u548cDMF\u6eb6\u6db2\uff08~2.7 M\uff09 \u203b\u989c\u8272\u53d8\u5316\u4e0d\u660e\u663e\u7684\u60c5\u51b5\u4e0b\uff0c\u4f7f\u7528N\uff0cN\u2019-\u4e8c\u7f9f\u57fa\u7126\u7f8e\u6797\u80fa\uff08NDHPI\uff09\u4ee3\u66ffNHPI\u53ef\u4ee5\u5f97\u5230\u6539\u5584\u3002 \u6b65\u9aa4 \u5c06\u5c11\u91cf\u56fa\u76f8\u5408\u6210\u6811\u8102\u52a0\u5165\u5fae\u7ba1\u4e2d\uff0c\u7528MeOH\u6e05\u6d173\u6b21\uff08\u53bb\u9664\u4f53\u7cfb\u91cc\u7684\u78b1\uff09\u3002 \u5c06\u8bd5\u6db2\u4e0e\u6811\u8102\u6df7\u5408\uff0c\u91cd\u590d\u6e05\u6d173\u6b21\u3002 \u5982\u679c\u6e05\u6d173\u6b21\u540e\u7684\u6811\u8102\u5448\u73b0\u7ea2\u8272\u81f3\u8910\u8272\u5219\u8868\u793a\u5b58\u5728\u672a\u53cd\u5e94\u7684\u672b\u7aef\u6c28\u57fa\uff0c\u5982\u679c\u6811\u8102\u5448\u73b0\u51fa\u65e0\u8272\u81f3\u9ec4\u8272\uff0c\u8868\u793a\u7f29\u5408\u53cd\u5e94\u5df2\u7ecf\u5168\u90e8\u8fdb\u884c\u5b8c\u6bd5\u3002 \uff08\u8ad6\u6587[5]SI\u3088\u308a\u5f15\u7528\uff09 (\u5f15\u7528\u81ea\u8bba\u6587[5]) \u53c2\u8003\u6587\u732e [1] \u201dColor Test for Detection of Free Terminal Amino Groups in the Solid-Phase Synthesis of Peptides\u201d Kaiser,E. T.; Colescott, R. L.; Bossinger, C. D.; Cook, P. I.\u00a0Anal. Biochem.\u00a01970,\u00a034, 595. doi: 10.1016\/0003-2697(70)90146-6. [2] \u201dQuantitative monitoring of solid-phase peptide synthesis by the ninhydrin reaction\u201d Sarin, V. K.; Kent, [&hellip;]","protected":false},"author":26,"featured_media":42340,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","enabled":false},"version":2}},"categories":[1458],"tags":[3852,3853],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"https:\/\/assets.www.gsbet888.com\/uploads\/2022\/07\/Kaiser_Test_0.png","jetpack_shortlink":"https:\/\/wp.me\/p96op1-b0a","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/posts\/42294"}],"collection":[{"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/users\/26"}],"replies":[{"embeddable":true,"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/comments?post=42294"}],"version-history":[{"count":4,"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/posts\/42294\/revisions"}],"predecessor-version":[{"id":42495,"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/posts\/42294\/revisions\/42495"}],"wp:featuredmedia":[{"embeddable":true,"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/media\/42340"}],"wp:attachment":[{"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/media?parent=42294"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/categories?post=42294"},{"taxonomy":"post_tag","embeddable":true,"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/tags?post=42294"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}