{"id":44669,"date":"2023-02-22T08:00:09","date_gmt":"2023-02-22T00:00:09","guid":{"rendered":"\/\/www.gsbet888.com\/?p=44669"},"modified":"2023-02-21T21:14:50","modified_gmt":"2023-02-21T13:14:50","slug":"%e5%8d%8e%e4%b8%ad%e7%a7%91%e5%a4%a7%e9%92%9f%e8%8a%b3%e9%94%90%e8%af%be%e9%a2%98%e7%bb%84%ef%bc%9a%e9%85%b6%e5%82%ac%e5%8c%96%e4%b8%8d%e5%af%b9%e7%a7%b0%e4%ba%a4%e5%8f%89%e6%b0%a7%e5%8c%96%e5%81%b6","status":"publish","type":"post","link":"\/\/www.gsbet888.com\/%e5%8c%96%e5%ad%a6%e6%9d%82%e8%ae%b0\/recentpaper\/2023\/02\/%e5%8d%8e%e4%b8%ad%e7%a7%91%e5%a4%a7%e9%92%9f%e8%8a%b3%e9%94%90%e8%af%be%e9%a2%98%e7%bb%84%ef%bc%9a%e9%85%b6%e5%82%ac%e5%8c%96%e4%b8%8d%e5%af%b9%e7%a7%b0%e4%ba%a4%e5%8f%89%e6%b0%a7%e5%8c%96%e5%81%b6.html","title":{"rendered":"\u534e\u4e2d\u79d1\u5927\u949f\u82b3\u9510\u8bfe\u9898\u7ec4\uff1a\u9176\u50ac\u5316\u4e0d\u5bf9\u79f0\u4ea4\u53c9\u6c27\u5316\u5076\u8054\u53cd\u5e94\u65b0\u8fdb\u5c55"},"content":{"rendered":"<p><strong><b>\u4f5c\u8005\uff1a\u77f3\u6cb9\u919a<\/b><\/strong><\/p>\n<h4><strong>\u5bfc\u8bfb\uff1a<\/strong><\/h4>\n<p>\u8fd1\u65e5\uff0c<strong><b>\u534e\u4e2d\u79d1\u6280\u5927\u5b66\u949f\u82b3\u9510\u6559\u6388<\/b><\/strong>\u56e2\u961f\u5728\u8457\u540d\u5b66\u672f\u671f\u520a<em><i>Angew. Chem. Int. Ed<\/i><\/em>.\u53d1\u8868\u8bba\u6587\uff0c\u62a5\u9053\u9176\u50ac\u5316\u4e0d\u5bf9\u79f0\u4ea4\u53c9\u6c27\u5316\u5076\u8054\u53cd\u5e94\u65b0\u8fdb\u5c55\u3002\u8be5\u53cd\u5e94\u5229\u7528\u94dc\u5916\u6392\u6c27\u5316\u9176(CueO)\u50ac\u5316\u5432\u54da\u7c7b\u5e95\u7269\u7684\u6c27\u5316\u4ea4\u53c9\u5076\u8054\u53cd\u5e94\uff0c\u5408\u6210\u4e86\u4e00\u7cfb\u5217\u5149\u5b66\u7eaf2,2-\u4e8c\u53d6\u4ee3\u5432\u54da\u5549-3-\u916e\u7c7b\u5316\u5408\u7269\uff0c\u5177\u6709\u6761\u4ef6\u6e29\u548c\u3001\u50ac\u5316\u5242\u7ecf\u6d4e\u3001\u9009\u62e9\u6027\u9ad8\u3001\u5e95\u7269\u666e\u9002\u6027\u597d\u7b49\u4f18\u70b9\uff0c\u6240\u5236\u5907\u7684\u5149\u5b66\u7eaf\u5432\u54da3-\u916e\u884d\u751f\u7269ee\u503c\u9ad8\u8fbe99%\u3002\u5728\u6b64\u57fa\u7840\u4e0a\uff0c\u4f5c\u8005\u7b5b\u9009\u4e86\u90e8\u5206\u4ea7\u7269\u7684\u751f\u7269\u6d3b\u6027\u7b5b\u9009\uff0c\u5f97\u5230\u4e86\u4e24\u4e2a\u5177\u6709\u663e\u8457\u6297\u80bf\u7624\u6d3b\u6027\u5206\u5b50\u3002<\/p>\n<blockquote><p>\u201cExpanding the Promiscuity of a Copper-Dependent Oxidase for Enantioselective Cross-Coupling of Indoles<\/p>\n<p>Huan Guo, Ningning Sun, Juan Guo, Tai-Ping Zhou, Langyu Tang, Wentao Zhang, Yaming Deng, Rong-Zhen Liao, Yuzhou Wu, Guojiao Wu, Fangrui Zhong*<\/p>\n<p><em><i>Angew. Chem. Int. Ed. <\/i><\/em>2023, ASAP. doi\uff1a<a href=\"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/anie.202219034\"><u>10.1002\/anie.202219034<\/u><\/a>\u201d<\/p><\/blockquote>\n<h4><strong>\u6b63\u6587\uff1a<\/strong><\/h4>\n<p>\u6c27\u5316\u4ea4\u53c9\u5076\u8054\u53cd\u5e94\u662f\u6709\u673a\u5408\u6210\u4e2d\u53d1\u5c55\u7684\u7eff\u8272\u9ad8\u6548\u5de5\u5177\uff0c\u53ef\u4ee5\u5c06\u4e24\u4e2a\u5bcc\u7535\u5b50\u4eb2\u6838\u8bd5\u5242\u76f4\u63a5\u5f62\u6210C-C\u952e\uff0c\u5177\u6709\u663e\u8457\u7684\u539f\u5b50\u7ecf\u6d4e\u6027\uff08\u56fe\u00a01\uff09\u3002\u5df2\u77e5\u7684\u4e0d\u5bf9\u79f0\u50ac\u5316\u4e3b\u8981\u662f\u57fa\u4e8e\u5316\u5b66\u6c27\u5316\u6216\u5149\u5316\u5b66\/\u7535\u5316\u5b66\u6c27\u5316\u4e0e\u624b\u6027\u8fc7\u6e21\u91d1\u5c5e\u6216\u6709\u673a\u50ac\u5316\u5242\u7684\u7ec4\u5408\uff0c\u5176\u6311\u6218\u5728\u4e8e\u540c\u65f6\u63a7\u5236\u6c27\u5316\u6b65\u9aa4\u7684\u5316\u5b66\u9009\u62e9\u6027\u548c\u5076\u8054\u6b65\u9aa4\u7684\u5bf9\u6620\u9009\u62e9\u6027\u3002\u6c27\u5316\u8fd8\u539f\u9176\u5177\u6709\u50ac\u5316\u4e0d\u5bf9\u79f0\u6c27\u5316\u5076\u8054\u7684\u5de8\u5927\u6f5c\u529b\uff0c\u5982\u81ea\u7136\u754c\u4e2d\uff0c\u6f06\u9176\u50ac\u5316\u7684\u03b3-\u8418\u916e\u540c\u5206\u5f02\u6784\u4f53(M)-ustilaginoidin A\u7684\u4e0d\u5bf9\u79f0\u540c\u6e90\u4e8c\u805a\u53cd\u5e94\uff0c\u5177\u6709\u4e13\u4e00\u7684\u533a\u57df\u548c\u5bf9\u6620\u9009\u62e9\u6027\uff08\u56fe\u00a01b\uff09\u3002\u7136\u800c\uff0c\u975e\u5929\u7136\u5e95\u7269\u7684\u9176\u4fc3\u5bf9\u6620\u9009\u62e9\u6027\u6c27\u5316\u5076\u8054\u53cd\u5e94\u4ecd\u7136\u5f88\u5c11\u30022022\u5e74\uff0cNarayan\u5c0f\u7ec4\u5229\u7528\u5de5\u7a0b\u5316P450\u9176\u5b9e\u73b0\u4e86\u915a\u7c7b\u5206\u5b50\u751f\u7269\u50ac\u5316\u4ea4\u53c9\u5076\u8054\u53cd\u5e94\uff0c\u4e3a\u8f74\u624b\u6027\u8054\u82b3\u57fa\u5206\u5b50\u7684\u5408\u6210\u63d0\u4f9b\u4e86\u6709\u6548\u7b56\u7565\u00a0(\u56fe1c)\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-44671 size-full\" src=\"https:\/\/assets.www.gsbet888.com\/uploads\/2023\/02\/8f35590e787256fe3e0b031564064a70.png\" alt=\"\" width=\"871\" height=\"923\" srcset=\"https:\/\/assets.www.gsbet888.com\/uploads\/2023\/02\/8f35590e787256fe3e0b031564064a70.png 871w, https:\/\/assets.www.gsbet888.com\/uploads\/2023\/02\/8f35590e787256fe3e0b031564064a70-283x300.png 283w, https:\/\/assets.www.gsbet888.com\/uploads\/2023\/02\/8f35590e787256fe3e0b031564064a70-680x721.png 680w, https:\/\/assets.www.gsbet888.com\/uploads\/2023\/02\/8f35590e787256fe3e0b031564064a70-768x814.png 768w\" sizes=\"(max-width: 871px) 100vw, 871px\" \/><\/p>\n<p style=\"text-align: center;\">\u56fe1. \u56fe\u7247\u6765\u6e90\uff1a\uff08<em><i>Angew. Chem. Int. Ed.<\/i><\/em>\uff09<\/p>\n<p>\u949f\u82b3\u9510\u6559\u6388\u957f\u671f\u81f4\u529b\u4e8e\u7eff\u8272\u4eff\u751f\u548c\u751f\u7269\u50ac\u5316\u9886\u57df\u7684\u7814\u7a76\uff0c\u5229\u7528\u5316\u5b66\u539f\u7406\u9a71\u52a8\u9176\u50ac\u5316\u9009\u62e9\u6027\u5408\u6210\u7684\u7406\u5ff5\uff0c\u57fa\u4e8e\u94dc\u50ac\u5316\u6c27\u5316\u5076\u8054\u53cd\u5e94\u7684\u5316\u5b66\u673a\u5236\uff0c\u53d1\u5c55\u4e86\u591a\u94dc\u6c27\u5316\u9176\u50ac\u5316\u7684\u591a\u915a\u6c27\u5316\u73af\u52a0\u6210\u53cd\u5e94\uff08<a href=\"https:\/\/pubs.rsc.org\/en\/Content\/ArticleLanding\/2022\/GC\/D2GC01706G\"><em><u><i>Green. Chem.,<\/i><\/u><\/em><u>\u00a0<\/u><strong><u><b>2022<\/b><\/u><\/strong><u>, 24, 5598.<\/u><\/a>\uff09\uff1b\u53d1\u5c55\u4e86\u542b\u6709\u975e\u5929\u7136\u5149\u654f\u6d3b\u6027\u57fa\u56e2\u7684\u4eba\u5de5\u5149\u654f\u91d1\u5c5e\u9176\uff08<a href=\"https:\/\/pubs.acs.org\/doi\/10.1021\/jacs.0c10882\"><em><u><i>J. Am. Chem. Soc.<\/i><\/u><\/em><u>\u00a0<\/u><strong><u><b>2021<\/b><\/u><\/strong><u>, 143, 617<\/u><\/a>\uff09\u548c\u201c\u4e09\u91cd\u6001\u5149\u9176\u201d\uff08<a href=\"https:\/\/www.nature.com\/articles\/s41586-022-05342-4\"><em><u><i>N<\/i><\/u><\/em><em><u><i>ature<\/i><\/u><\/em><u>, <\/u><strong><u><b>2022<\/b><\/u><\/strong><u>, 611<\/u><u>,<\/u><u>\u00a0715<\/u><\/a>\uff09\u3002<\/p>\n<p>\u5728\u6b64\u524d\u5de5\u4f5c\u57fa\u7840\u4e0a\uff0c\u8be5\u8bfe\u9898\u7ec4\u6700\u8fd1\u5f00\u5c55\u4e86\u94dc\u9176\u50ac\u5316\u975e\u5929\u7136\u5e95\u7269\u7684\u4e0d\u5bf9\u79f0\u6c27\u5316\u4ea4\u53c9\u5076\u8054\u53cd\u5e94\u7684\u7814\u7a76\u5e76\u53d6\u5f97\u91cd\u8981\u8fdb\u5c55\uff0c\u5728\u6e29\u548c\u7684\u6c34\u76f8\u6761\u4ef6\u4e0b\u5b9e\u73b0\u4e86\u94dc\u9176CueO\u50ac\u5316\u5432\u54da\u915a\u4e0e\u4e00\u7cfb\u5217\u4eb2\u6838\u8bd5\u5242\u7684\u5bf9\u6620\u9009\u62e9\u6027\u6c27\u5316\u4ea4\u53c9\u5076\u8054\uff0c\u6210\u529f\u5e94\u7528\u4e8e\u5149\u5b66\u7eaf2,2-\u4e8c\u53d6\u4ee3\u5432\u54da\u5549-3-\u916e\u7c7b\u5316\u5408\u7269\u7684\u624b\u6027\u5408\u6210\uff08\u56fe 1d\uff09\uff0c\u5e76\u5bf9\u9176\u4fc3\u53cd\u5e94\u673a\u7406\u8fdb\u884c\u4e86\u521d\u6b65\u63a2\u8ba8\u3002<\/p>\n<p>\u8be5\u56e2\u961f\u4ee5\u5927\u80a0\u6746\u83cc\u57f9\u517b\u8868\u8fbe\u7684\u94dc\u9176CueO\u88c2\u89e3\u6db2\u4e3a\u50ac\u5316\u5242\uff0c\u4ee53-\u7f9f\u57fa\u5432\u54da-2-\u7fa7\u9178\u548c\u5432\u54da\u4e3a\u6a21\u578b\u53cd\u5e94\uff0c\u6700\u4f18\u6761\u4ef6\u4e0b\uff080.1 mol\uff05 CueO\uff0c5.0\u5f53\u91cf\u53cc\u6c27\u6c34\uff0cTris\/CH3CN\u4e3a\u6eb6\u5242\uff0c25\u2103\uff0c24\u5c0f\u65f6\uff09\u53ef\u4ee5\u5f97\u5230\u76ee\u6807\u4ea7\u7269\uff0c\u6536\u7387\u4e3a92\uff05\uff0c\u5bf9\u6620\u9009\u62e9\u6027\u4e3a99\uff05\u3002\u9176\u7684\u7528\u91cf\u8f83\u4f4e\uff0c\u53cd\u5e94\u8f6c\u5316\u6570\u4e86920.<\/p>\n<p>\u5728\u6700\u4f18\u6761\u4ef6\u4e0b\uff0c\u4f5c\u8005\u63a2\u7d22\u4e86\u6b64\u7c7b\u53cd\u5e94\u7684\u5e95\u7269\u666e\u9002\u6027\u548c\u5b98\u80fd\u56e2\u517c\u5bb9\u6027\uff08\u56fe2\uff09\u3002\u82ef\u73af\u4e0a\u542b\u6709\u4e0d\u540c\u7535\u5b50\u548c\u7acb\u4f53\u6027\u8d28\u53d6\u4ee3\u57fa\u7684\u5432\u54da\u915a\u80fd\u987a\u5229\u53d1\u751f\u9884\u671f\u53cd\u5e94\uff0c\u6536\u7387\u4e3a73-78\uff05\uff0c\u7acb\u4f53\u9009\u62e9\u6027\u4e00\u822c\u90fd\u4e3a99\uff05ee\u3002\u6b64\u5916\uff0c\u4e0d\u540c\u4f4d\u7f6e\u53d6\u4ee3\u7684\u5432\u54da\u4eb2\u6838\u8bd5\u5242\u3001\u5bcc\u7535\u5b50\u7c7b\u5421\u54af\u30011,3,5-\u4e09\u7532\u6c27\u57fa\u82ef\u548c1,3-\u4e8c\u7532\u6c27\u57fa\u82ef\u4e5f\u540c\u6837\u9002\u7528\u3002\u4eb2\u6838\u8bd5\u5242\u7684\u8303\u56f4\u8fd8\u53ef\u4ee5\u8fdb\u4e00\u6b65\u6269\u5c55\u5230<em><i>sp<\/i><\/em><em><sup><i>3<\/i><\/sup><\/em>\u6742\u5316\u7684\u78b3\u4eb2\u6838\u8bd5\u5242\uff0c\u5982\u73af\u72b6\u548c\u975e\u73af\u72b6\u03b2-\u916e\u9178\u916f\uff0c\u76f8\u5e94\u7684\u4ea7\u7269\u5747\u5177\u6709\u6781\u597d\u7684\u7acb\u4f53\u9009\u62e9\u6027\u3002\u90e8\u5206\u5e95\u7269\u7684\u653e\u5927\u5b9e\u9a8c\uff080.3-1.0 mmol\uff09\u53d1\u73b0\uff0c\u4ea7\u7269\u7684\u4ea7\u7387\u548c\u5149\u5b66\u7eaf\u5ea6\u4e0d\u53d7\u5f71\u54cd\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-44672 size-full\" src=\"https:\/\/assets.www.gsbet888.com\/uploads\/2023\/02\/99d1716cc00cf6fb47f67f4b5ffef5e0.png\" alt=\"\" width=\"1003\" height=\"747\" srcset=\"https:\/\/assets.www.gsbet888.com\/uploads\/2023\/02\/99d1716cc00cf6fb47f67f4b5ffef5e0.png 1003w, https:\/\/assets.www.gsbet888.com\/uploads\/2023\/02\/99d1716cc00cf6fb47f67f4b5ffef5e0-300x223.png 300w, https:\/\/assets.www.gsbet888.com\/uploads\/2023\/02\/99d1716cc00cf6fb47f67f4b5ffef5e0-680x506.png 680w, https:\/\/assets.www.gsbet888.com\/uploads\/2023\/02\/99d1716cc00cf6fb47f67f4b5ffef5e0-768x572.png 768w, https:\/\/assets.www.gsbet888.com\/uploads\/2023\/02\/99d1716cc00cf6fb47f67f4b5ffef5e0-265x197.png 265w\" sizes=\"(max-width: 1003px) 100vw, 1003px\" \/><\/p>\n<p style=\"text-align: center;\">\u56fe2.\u00a0\u4ee3\u8868\u6027\u5e95\u7269<\/p>\n<p>\u7814\u7a76\u56e2\u961f\u5bf9\u8be5\u9176\u4fc3\u53cd\u5e94\u7684\u673a\u7406\u8fdb\u884c\u4e86\u6df1\u5165\u7684\u7814\u7a76\uff08\u56fe3\uff09\u3002\u9996\u5148\u901a\u8fc7\u63a7\u5236\u5b9e\u9a8c\u6355\u83b7\u548c\u9a8c\u8bc1\u4e86\u4e2d\u95f4\u4f53\u5206\u5b50\u7684\u751f\u6210\u3002\u5728\u6b64\u57fa\u7840\u4e0a\uff0c\u901a\u8fc7\u94dc\u7684\u914d\u4f4d\u73af\u5883\u5206\u6790\u3001\u4e2d\u95f4\u4f53\u5206\u5b50\u4e0e\u9176\u7684\u5206\u5b50\u5bf9\u63a5\u6a21\u62df\u7b49\u5b9e\u9a8c\uff0c\u63d0\u51fa\u4e86\u5e95\u7269\u4e0e\u9176\u7684\u53ef\u80fd\u4f5c\u7528\u6a21\u578b\uff1a\u4e2d\u95f4\u4f53\u7684\u916f\u57fa\u4e0eCueO\u9176Asn240\u7684\u9170\u80faN-H\u548cLys188\u7684\u6c28\u57fa\u5b58\u5728\u7740\u5f31\u6c22\u952e\u4f5c\u7528\uff1b\u8fd9\u4e9b\u76f8\u4e92\u4f5c\u7528\u53ef\u80fd\u5bf9\u4ea4\u53c9\u5076\u8054\u53cd\u5e94\u7684\u53cd\u5e94\u6027\u548c\u5bf9\u6620\u9009\u62e9\u6027\u81f3\u5173\u91cd\u8981\u3002\u5f53\u5c06\u8fd9\u4e24\u4e2a\u6c28\u57fa\u9178\u6b8b\u57fa\u7a81\u53d8\u6210\u4e19\u6c28\u9178\uff0c\u6240\u5f97\u7a81\u53d8\u4f53CueO_K188A\u548cCueO_N240A\u7684\u50ac\u5316\u6548\u679c\u663e\u8457\u964d\u4f4e\u3002\u8fd9\u4e00\u7ed3\u679c\u9a8c\u8bc1\u4e86\u4e0a\u8ff0\u63a8\u6d4b\u7684\u9176\u4f5c\u7528\u65b9\u5f0f\u7684\u5408\u7406\u6027\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-44673 size-full\" src=\"https:\/\/assets.www.gsbet888.com\/uploads\/2023\/02\/9c9a0a82729cfcdec8f958887eccf1f0.png\" alt=\"\" width=\"981\" height=\"1407\" srcset=\"https:\/\/assets.www.gsbet888.com\/uploads\/2023\/02\/9c9a0a82729cfcdec8f958887eccf1f0.png 981w, https:\/\/assets.www.gsbet888.com\/uploads\/2023\/02\/9c9a0a82729cfcdec8f958887eccf1f0-209x300.png 209w, https:\/\/assets.www.gsbet888.com\/uploads\/2023\/02\/9c9a0a82729cfcdec8f958887eccf1f0-680x975.png 680w, https:\/\/assets.www.gsbet888.com\/uploads\/2023\/02\/9c9a0a82729cfcdec8f958887eccf1f0-768x1102.png 768w\" sizes=\"(max-width: 981px) 100vw, 981px\" \/><\/p>\n<p style=\"text-align: center;\">\u56fe3. \u56fe\u7247\u6765\u6e90\uff1a\uff08<em><i>Angew. Chem. Int. Ed.<\/i><\/em>\uff09<\/p>\n<p>\u76f8\u5173\u6587\u732e\u8868\u660e\uff0c2,2-\u4e8c\u53d6\u4ee3\u5432\u54da\u5549-3-\u916e\u7c7b\u5206\u5b50\u53ef\u80fd\u5177\u6709\u663e\u8457\u7684\u751f\u7269\u6d3b\u6027\u3002\u8be5\u56e2\u961f\u5229\u7528MTT\u6cd5\u6d4b\u5b9a\u4e86\u90e8\u5206\u4ea7\u7269\u5bf9MCF-7\u80bf\u7624\u7ec6\u80de\u7cfb\u7684\u6bd2\u6027\uff0c\u5f97\u5230\u4e86\u4e24\u4e2a\u5177\u6709\u663e\u8457\u6297\u764c\u6d3b\u6027\u7684\u5316\u5408\u72693f\u548c4a\uff08\u6d53\u5ea6\u4e3a50\u03bcM\uff09\uff08\u56fe4\uff09\u3002\u8fdb\u4e00\u6b65\u5728\u4e0d\u540c\u6d53\u5ea6\u4e0b\u7684\u6bd2\u6027\u6d4b\u8bd5\u5b9e\u9a8c\u62df\u5408\u5f97\u5230IC<sub>50<\/sub>\u503c\u5206\u522b\u4e3a34.31 \u03bcM\u548c37.2 \u03bcM\uff0c\u5747\u9ad8\u4e8e\u4e34\u5e8a\u836f\u7269\u4f0a\u7acb\u66ff\u5eb7\u548c5-\u6c1f\u5c3f\u5627\u5576\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-44674 size-full\" src=\"https:\/\/assets.www.gsbet888.com\/uploads\/2023\/02\/3f716e057b367393498b35842591d219.png\" alt=\"\" width=\"918\" height=\"747\" srcset=\"https:\/\/assets.www.gsbet888.com\/uploads\/2023\/02\/3f716e057b367393498b35842591d219.png 918w, https:\/\/assets.www.gsbet888.com\/uploads\/2023\/02\/3f716e057b367393498b35842591d219-300x244.png 300w, https:\/\/assets.www.gsbet888.com\/uploads\/2023\/02\/3f716e057b367393498b35842591d219-680x553.png 680w, https:\/\/assets.www.gsbet888.com\/uploads\/2023\/02\/3f716e057b367393498b35842591d219-768x625.png 768w\" sizes=\"(max-width: 918px) 100vw, 918px\" \/><\/p>\n<p style=\"text-align: center;\">\u56fe4\u00a0\u56fe\u7247\u6765\u6e90\uff1a\uff08<em><i>Angew. Chem. Int. Ed.<\/i><\/em>\uff09<\/p>\n<h4><strong><b>\u603b\u7ed3<\/b><\/strong><\/h4>\n<p>\u603b\u4e4b\uff0c\u949f\u82b3\u9510\u6559\u6388\u8bfe\u9898\u7ec4\u4ee5\u57fa\u4e8e\u94dc\u50ac\u5316\u7684\u4ea4\u53c9\u5076\u8054\u53cd\u5e94\u5316\u5b66\u673a\u5236\uff0c\u4ee5\u94dc\u9176CueO\u4e3a\u751f\u7269\u50ac\u5316\u5242\uff0c\u4ee5H<sub>2<\/sub>O<sub>2<\/sub>\u4e3a\u7ec8\u7aef\u6c27\u5316\u5242\u5efa\u7acb\u4e86\u4e00\u79cd\u4e0d\u540c\u5432\u54da\u5206\u5b50\u95f4\u7684\u5bf9\u6620\u9009\u62e9\u6027\u6c27\u5316\u4ea4\u53c9\u5076\u8054\u53cd\u5e94\uff0c\u5177\u6709\u64cd\u4f5c\u7b80\u4fbf\u3001\u9ad8\u539f\u5b50\u7ecf\u6d4e\u6027\u3001\u8f83\u597d\u7684\u5e95\u7269\u666e\u9002\u6027\u7b49\u7279\u70b9\u3002\u8be5\u65b9\u6cd5\u63d0\u4f9b\u4e86\u4e00\u79cd\u5149\u5b66\u7eaf2,2-\u4e8c\u53d6\u4ee3\u5432\u54da\u916e\u7684\u7eff\u8272\u5408\u6210\u65b9\u6cd5\uff0c\u5e76\u7b5b\u9009\u5f97\u5230\u4e86\u4e24\u4e2a\u663e\u8457\u6297\u764c\u6d3b\u6027\u5206\u5b50\u3002\u8fd9\u9879\u5de5\u4f5c\u663e\u8457\u62d3\u5c55\u4e86\u591a\u94dc\u6c27\u5316\u9176\u7684\u50ac\u5316\u6742\u6cdb\u6027\uff0c\u5c06\u4e3a\u53d1\u5c55\u94dc\u9176\u50ac\u5316\u7684\u5176\u5b83\u975e\u5929\u7136\u6c27\u5316\u53cd\u5e94\u5e26\u6765\u542f\u53d1\u3002<\/p>\n<p>\u672c\u6587\u7248\u6743\u5c5e\u4e8e\u00a0Chem-Station\u5316\u5b66\u7a7a\u95f4\uff0c \u6b22\u8fce\u70b9\u51fb\u6309\u94ae\u5206\u4eab\uff0c\u672a\u7ecf\u8bb8\u53ef\uff0c\u8c22\u7edd\u8f6c\u8f7d<\/p>\n","protected":false},"excerpt":{"rendered":"\u4f5c\u8005\uff1a\u77f3\u6cb9\u919a \u5bfc\u8bfb\uff1a \u8fd1\u65e5\uff0c\u534e\u4e2d\u79d1\u6280\u5927\u5b66\u949f\u82b3\u9510\u6559\u6388\u56e2\u961f\u5728\u8457\u540d\u5b66\u672f\u671f\u520aAngew. Chem. Int. Ed.\u53d1\u8868\u8bba\u6587\uff0c\u62a5\u9053\u9176\u50ac\u5316\u4e0d\u5bf9\u79f0\u4ea4\u53c9\u6c27\u5316\u5076\u8054\u53cd\u5e94\u65b0\u8fdb\u5c55\u3002\u8be5\u53cd\u5e94\u5229\u7528\u94dc\u5916\u6392\u6c27\u5316\u9176(CueO)\u50ac\u5316\u5432\u54da\u7c7b\u5e95\u7269\u7684\u6c27\u5316\u4ea4\u53c9\u5076\u8054\u53cd\u5e94\uff0c\u5408\u6210\u4e86\u4e00\u7cfb\u5217\u5149\u5b66\u7eaf2,2-\u4e8c\u53d6\u4ee3\u5432\u54da\u5549-3-\u916e\u7c7b\u5316\u5408\u7269\uff0c\u5177\u6709\u6761\u4ef6\u6e29\u548c\u3001\u50ac\u5316\u5242\u7ecf\u6d4e\u3001\u9009\u62e9\u6027\u9ad8\u3001\u5e95\u7269\u666e\u9002\u6027\u597d\u7b49\u4f18\u70b9\uff0c\u6240\u5236\u5907\u7684\u5149\u5b66\u7eaf\u5432\u54da3-\u916e\u884d\u751f\u7269ee\u503c\u9ad8\u8fbe99%\u3002\u5728\u6b64\u57fa\u7840\u4e0a\uff0c\u4f5c\u8005\u7b5b\u9009\u4e86\u90e8\u5206\u4ea7\u7269\u7684\u751f\u7269\u6d3b\u6027\u7b5b\u9009\uff0c\u5f97\u5230\u4e86\u4e24\u4e2a\u5177\u6709\u663e\u8457\u6297\u80bf\u7624\u6d3b\u6027\u5206\u5b50\u3002 \u201cExpanding the Promiscuity of a Copper-Dependent Oxidase for Enantioselective Cross-Coupling of Indoles Huan Guo, Ningning Sun, Juan Guo, Tai-Ping Zhou, Langyu Tang, Wentao Zhang, Yaming Deng, Rong-Zhen Liao, Yuzhou Wu, Guojiao Wu, Fangrui Zhong* Angew. Chem. Int. Ed. 2023, ASAP. doi\uff1a10.1002\/anie.202219034\u201d \u6b63\u6587\uff1a \u6c27\u5316\u4ea4\u53c9\u5076\u8054\u53cd\u5e94\u662f\u6709\u673a\u5408\u6210\u4e2d\u53d1\u5c55\u7684\u7eff\u8272\u9ad8\u6548\u5de5\u5177\uff0c\u53ef\u4ee5\u5c06\u4e24\u4e2a\u5bcc\u7535\u5b50\u4eb2\u6838\u8bd5\u5242\u76f4\u63a5\u5f62\u6210C-C\u952e\uff0c\u5177\u6709\u663e\u8457\u7684\u539f\u5b50\u7ecf\u6d4e\u6027\uff08\u56fe\u00a01\uff09\u3002\u5df2\u77e5\u7684\u4e0d\u5bf9\u79f0\u50ac\u5316\u4e3b\u8981\u662f\u57fa\u4e8e\u5316\u5b66\u6c27\u5316\u6216\u5149\u5316\u5b66\/\u7535\u5316\u5b66\u6c27\u5316\u4e0e\u624b\u6027\u8fc7\u6e21\u91d1\u5c5e\u6216\u6709\u673a\u50ac\u5316\u5242\u7684\u7ec4\u5408\uff0c\u5176\u6311\u6218\u5728\u4e8e\u540c\u65f6\u63a7\u5236\u6c27\u5316\u6b65\u9aa4\u7684\u5316\u5b66\u9009\u62e9\u6027\u548c\u5076\u8054\u6b65\u9aa4\u7684\u5bf9\u6620\u9009\u62e9\u6027\u3002\u6c27\u5316\u8fd8\u539f\u9176\u5177\u6709\u50ac\u5316\u4e0d\u5bf9\u79f0\u6c27\u5316\u5076\u8054\u7684\u5de8\u5927\u6f5c\u529b\uff0c\u5982\u81ea\u7136\u754c\u4e2d\uff0c\u6f06\u9176\u50ac\u5316\u7684\u03b3-\u8418\u916e\u540c\u5206\u5f02\u6784\u4f53(M)-ustilaginoidin A\u7684\u4e0d\u5bf9\u79f0\u540c\u6e90\u4e8c\u805a\u53cd\u5e94\uff0c\u5177\u6709\u4e13\u4e00\u7684\u533a\u57df\u548c\u5bf9\u6620\u9009\u62e9\u6027\uff08\u56fe\u00a01b\uff09\u3002\u7136\u800c\uff0c\u975e\u5929\u7136\u5e95\u7269\u7684\u9176\u4fc3\u5bf9\u6620\u9009\u62e9\u6027\u6c27\u5316\u5076\u8054\u53cd\u5e94\u4ecd\u7136\u5f88\u5c11\u30022022\u5e74\uff0cNarayan\u5c0f\u7ec4\u5229\u7528\u5de5\u7a0b\u5316P450\u9176\u5b9e\u73b0\u4e86\u915a\u7c7b\u5206\u5b50\u751f\u7269\u50ac\u5316\u4ea4\u53c9\u5076\u8054\u53cd\u5e94\uff0c\u4e3a\u8f74\u624b\u6027\u8054\u82b3\u57fa\u5206\u5b50\u7684\u5408\u6210\u63d0\u4f9b\u4e86\u6709\u6548\u7b56\u7565\u00a0(\u56fe1c)\u3002 \u56fe1. \u56fe\u7247\u6765\u6e90\uff1a\uff08Angew. Chem. Int. Ed.\uff09 \u949f\u82b3\u9510\u6559\u6388\u957f\u671f\u81f4\u529b\u4e8e\u7eff\u8272\u4eff\u751f\u548c\u751f\u7269\u50ac\u5316\u9886\u57df\u7684\u7814\u7a76\uff0c\u5229\u7528\u5316\u5b66\u539f\u7406\u9a71\u52a8\u9176\u50ac\u5316\u9009\u62e9\u6027\u5408\u6210\u7684\u7406\u5ff5\uff0c\u57fa\u4e8e\u94dc\u50ac\u5316\u6c27\u5316\u5076\u8054\u53cd\u5e94\u7684\u5316\u5b66\u673a\u5236\uff0c\u53d1\u5c55\u4e86\u591a\u94dc\u6c27\u5316\u9176\u50ac\u5316\u7684\u591a\u915a\u6c27\u5316\u73af\u52a0\u6210\u53cd\u5e94\uff08Green. Chem.,\u00a02022, 24, 5598.\uff09\uff1b\u53d1\u5c55\u4e86\u542b\u6709\u975e\u5929\u7136\u5149\u654f\u6d3b\u6027\u57fa\u56e2\u7684\u4eba\u5de5\u5149\u654f\u91d1\u5c5e\u9176\uff08J. Am. Chem. Soc.\u00a02021, 143, 617\uff09\u548c\u201c\u4e09\u91cd\u6001\u5149\u9176\u201d\uff08Nature, 2022, 611,\u00a0715\uff09\u3002 \u5728\u6b64\u524d\u5de5\u4f5c\u57fa\u7840\u4e0a\uff0c\u8be5\u8bfe\u9898\u7ec4\u6700\u8fd1\u5f00\u5c55\u4e86\u94dc\u9176\u50ac\u5316\u975e\u5929\u7136\u5e95\u7269\u7684\u4e0d\u5bf9\u79f0\u6c27\u5316\u4ea4\u53c9\u5076\u8054\u53cd\u5e94\u7684\u7814\u7a76\u5e76\u53d6\u5f97\u91cd\u8981\u8fdb\u5c55\uff0c\u5728\u6e29\u548c\u7684\u6c34\u76f8\u6761\u4ef6\u4e0b\u5b9e\u73b0\u4e86\u94dc\u9176CueO\u50ac\u5316\u5432\u54da\u915a\u4e0e\u4e00\u7cfb\u5217\u4eb2\u6838\u8bd5\u5242\u7684\u5bf9\u6620\u9009\u62e9\u6027\u6c27\u5316\u4ea4\u53c9\u5076\u8054\uff0c\u6210\u529f\u5e94\u7528\u4e8e\u5149\u5b66\u7eaf2,2-\u4e8c\u53d6\u4ee3\u5432\u54da\u5549-3-\u916e\u7c7b\u5316\u5408\u7269\u7684\u624b\u6027\u5408\u6210\uff08\u56fe 1d\uff09\uff0c\u5e76\u5bf9\u9176\u4fc3\u53cd\u5e94\u673a\u7406\u8fdb\u884c\u4e86\u521d\u6b65\u63a2\u8ba8\u3002 \u8be5\u56e2\u961f\u4ee5\u5927\u80a0\u6746\u83cc\u57f9\u517b\u8868\u8fbe\u7684\u94dc\u9176CueO\u88c2\u89e3\u6db2\u4e3a\u50ac\u5316\u5242\uff0c\u4ee53-\u7f9f\u57fa\u5432\u54da-2-\u7fa7\u9178\u548c\u5432\u54da\u4e3a\u6a21\u578b\u53cd\u5e94\uff0c\u6700\u4f18\u6761\u4ef6\u4e0b\uff080.1 mol\uff05 CueO\uff0c5.0\u5f53\u91cf\u53cc\u6c27\u6c34\uff0cTris\/CH3CN\u4e3a\u6eb6\u5242\uff0c25\u2103\uff0c24\u5c0f\u65f6\uff09\u53ef\u4ee5\u5f97\u5230\u76ee\u6807\u4ea7\u7269\uff0c\u6536\u7387\u4e3a92\uff05\uff0c\u5bf9\u6620\u9009\u62e9\u6027\u4e3a99\uff05\u3002\u9176\u7684\u7528\u91cf\u8f83\u4f4e\uff0c\u53cd\u5e94\u8f6c\u5316\u6570\u4e86920. \u5728\u6700\u4f18\u6761\u4ef6\u4e0b\uff0c\u4f5c\u8005\u63a2\u7d22\u4e86\u6b64\u7c7b\u53cd\u5e94\u7684\u5e95\u7269\u666e\u9002\u6027\u548c\u5b98\u80fd\u56e2\u517c\u5bb9\u6027\uff08\u56fe2\uff09\u3002\u82ef\u73af\u4e0a\u542b\u6709\u4e0d\u540c\u7535\u5b50\u548c\u7acb\u4f53\u6027\u8d28\u53d6\u4ee3\u57fa\u7684\u5432\u54da\u915a\u80fd\u987a\u5229\u53d1\u751f\u9884\u671f\u53cd\u5e94\uff0c\u6536\u7387\u4e3a73-78\uff05\uff0c\u7acb\u4f53\u9009\u62e9\u6027\u4e00\u822c\u90fd\u4e3a99\uff05ee\u3002\u6b64\u5916\uff0c\u4e0d\u540c\u4f4d\u7f6e\u53d6\u4ee3\u7684\u5432\u54da\u4eb2\u6838\u8bd5\u5242\u3001\u5bcc\u7535\u5b50\u7c7b\u5421\u54af\u30011,3,5-\u4e09\u7532\u6c27\u57fa\u82ef\u548c1,3-\u4e8c\u7532\u6c27\u57fa\u82ef\u4e5f\u540c\u6837\u9002\u7528\u3002\u4eb2\u6838\u8bd5\u5242\u7684\u8303\u56f4\u8fd8\u53ef\u4ee5\u8fdb\u4e00\u6b65\u6269\u5c55\u5230sp3\u6742\u5316\u7684\u78b3\u4eb2\u6838\u8bd5\u5242\uff0c\u5982\u73af\u72b6\u548c\u975e\u73af\u72b6\u03b2-\u916e\u9178\u916f\uff0c\u76f8\u5e94\u7684\u4ea7\u7269\u5747\u5177\u6709\u6781\u597d\u7684\u7acb\u4f53\u9009\u62e9\u6027\u3002\u90e8\u5206\u5e95\u7269\u7684\u653e\u5927\u5b9e\u9a8c\uff080.3-1.0 mmol\uff09\u53d1\u73b0\uff0c\u4ea7\u7269\u7684\u4ea7\u7387\u548c\u5149\u5b66\u7eaf\u5ea6\u4e0d\u53d7\u5f71\u54cd\u3002 \u56fe2.\u00a0\u4ee3\u8868\u6027\u5e95\u7269 \u7814\u7a76\u56e2\u961f\u5bf9\u8be5\u9176\u4fc3\u53cd\u5e94\u7684\u673a\u7406\u8fdb\u884c\u4e86\u6df1\u5165\u7684\u7814\u7a76\uff08\u56fe3\uff09\u3002\u9996\u5148\u901a\u8fc7\u63a7\u5236\u5b9e\u9a8c\u6355\u83b7\u548c\u9a8c\u8bc1\u4e86\u4e2d\u95f4\u4f53\u5206\u5b50\u7684\u751f\u6210\u3002\u5728\u6b64\u57fa\u7840\u4e0a\uff0c\u901a\u8fc7\u94dc\u7684\u914d\u4f4d\u73af\u5883\u5206\u6790\u3001\u4e2d\u95f4\u4f53\u5206\u5b50\u4e0e\u9176\u7684\u5206\u5b50\u5bf9\u63a5\u6a21\u62df\u7b49\u5b9e\u9a8c\uff0c\u63d0\u51fa\u4e86\u5e95\u7269\u4e0e\u9176\u7684\u53ef\u80fd\u4f5c\u7528\u6a21\u578b\uff1a\u4e2d\u95f4\u4f53\u7684\u916f\u57fa\u4e0eCueO\u9176Asn240\u7684\u9170\u80faN-H\u548cLys188\u7684\u6c28\u57fa\u5b58\u5728\u7740\u5f31\u6c22\u952e\u4f5c\u7528\uff1b\u8fd9\u4e9b\u76f8\u4e92\u4f5c\u7528\u53ef\u80fd\u5bf9\u4ea4\u53c9\u5076\u8054\u53cd\u5e94\u7684\u53cd\u5e94\u6027\u548c\u5bf9\u6620\u9009\u62e9\u6027\u81f3\u5173\u91cd\u8981\u3002\u5f53\u5c06\u8fd9\u4e24\u4e2a\u6c28\u57fa\u9178\u6b8b\u57fa\u7a81\u53d8\u6210\u4e19\u6c28\u9178\uff0c\u6240\u5f97\u7a81\u53d8\u4f53CueO_K188A\u548cCueO_N240A\u7684\u50ac\u5316\u6548\u679c\u663e\u8457\u964d\u4f4e\u3002\u8fd9\u4e00\u7ed3\u679c\u9a8c\u8bc1\u4e86\u4e0a\u8ff0\u63a8\u6d4b\u7684\u9176\u4f5c\u7528\u65b9\u5f0f\u7684\u5408\u7406\u6027\u3002 \u56fe3. \u56fe\u7247\u6765\u6e90\uff1a\uff08Angew. Chem. Int. Ed.\uff09 \u76f8\u5173\u6587\u732e\u8868\u660e\uff0c2,2-\u4e8c\u53d6\u4ee3\u5432\u54da\u5549-3-\u916e\u7c7b\u5206\u5b50\u53ef\u80fd\u5177\u6709\u663e\u8457\u7684\u751f\u7269\u6d3b\u6027\u3002\u8be5\u56e2\u961f\u5229\u7528MTT\u6cd5\u6d4b\u5b9a\u4e86\u90e8\u5206\u4ea7\u7269\u5bf9MCF-7\u80bf\u7624\u7ec6\u80de\u7cfb\u7684\u6bd2\u6027\uff0c\u5f97\u5230\u4e86\u4e24\u4e2a\u5177\u6709\u663e\u8457\u6297\u764c\u6d3b\u6027\u7684\u5316\u5408\u72693f\u548c4a\uff08\u6d53\u5ea6\u4e3a50\u03bcM\uff09\uff08\u56fe4\uff09\u3002\u8fdb\u4e00\u6b65\u5728\u4e0d\u540c\u6d53\u5ea6\u4e0b\u7684\u6bd2\u6027\u6d4b\u8bd5\u5b9e\u9a8c\u62df\u5408\u5f97\u5230IC50\u503c\u5206\u522b\u4e3a34.31 \u03bcM\u548c37.2 \u03bcM\uff0c\u5747\u9ad8\u4e8e\u4e34\u5e8a\u836f\u7269\u4f0a\u7acb\u66ff\u5eb7\u548c5-\u6c1f\u5c3f\u5627\u5576\u3002 \u56fe4\u00a0\u56fe\u7247\u6765\u6e90\uff1a\uff08Angew. Chem. Int. 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