{"id":46574,"date":"2023-08-12T08:00:57","date_gmt":"2023-08-12T00:00:57","guid":{"rendered":"\/\/www.gsbet888.com\/?p=46574"},"modified":"2023-08-10T23:16:31","modified_gmt":"2023-08-10T15:16:31","slug":"%e8%9b%8b%e7%99%bd%e8%b4%a8%e5%ae%9a%e9%87%8f-%e5%b8%83%e6%8b%89%e5%be%b7%e7%a6%8f%e8%9b%8b%e7%99%bd%e8%b4%a8%e5%ae%9a%e9%87%8f%e6%b3%95bradford-protein-assay","status":"publish","type":"post","link":"\/\/www.gsbet888.com\/%e5%8c%96%e5%ad%a6%e6%9d%82%e8%ae%b0\/%e5%ae%9e%e9%aa%8c%e6%8a%80%e5%b7%a7\/2023\/08\/%e8%9b%8b%e7%99%bd%e8%b4%a8%e5%ae%9a%e9%87%8f-%e5%b8%83%e6%8b%89%e5%be%b7%e7%a6%8f%e8%9b%8b%e7%99%bd%e8%b4%a8%e5%ae%9a%e9%87%8f%e6%b3%95bradford-protein-assay.html","title":{"rendered":"\u86cb\u767d\u8d28\u5b9a\u91cf-\u5e03\u62c9\u5fb7\u798f\u86cb\u767d\u8d28\u5b9a\u91cf\u6cd5Bradford Protein Assay"},"content":{"rendered":"<p>\u8bd1\u81eaChem-Station\u7f51\u7ad9\u65e5\u672c\u7248\u00a0\u539f\u6587\u94fe\u63a5\uff1a<a href=\"https:\/\/www.chem-station.com\/blog\/2022\/10\/pq5.html\"><u>\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5b9a\u91cf\u6cd5\u2015\u30d6\u30e9\u30c3\u30c9\u30d5\u30a9\u30fc\u30c9\u6cd5 Protein Quantification \u2013 Bradford Protein Assay<\/u><\/a><\/p>\n<p>\u7ffb\u8bd1\uff1a\u70b8\u9e21<\/p>\n<h4><strong><b>\u539f\u7406<\/b><\/strong><\/h4>\n<p><strong><b>\u5e03\u62c9\u5fb7\u798f\u86cb\u767d\u8d28\u5b9a\u91cf\u6cd5<\/b><\/strong><strong><b>\uff08<\/b><\/strong><strong><b>Bradford Protein Assary<\/b><\/strong><strong><b>\uff09<\/b><\/strong>\u4e0eBCA\u6cd5\u5e76\u5217\u4e3a\u6700\u5e38\u4f7f\u7528\u7684\u86cb\u767d\u8d28\u6d53\u5ea6\u5b9a\u91cf\u6cd5\u3002<\/p>\n<p>\u539f\u7406\u662f\u8003\u9a6c\u65af\u4eae\u84dd\uff08CBB\uff09 G-250\u8fd9\u79cd\u8272\u7d20\u4f1a\u4e0e\u86cb\u767d\u8d28\u7684\u78b1\u6027\u4e14\u5c5e\u4e8e\u82b3\u9999\u65cf\u7684\u6c28\u57fa\u9178\u7684\u4fa7\u94fe\u76f8\u4e92\u4f5c\u7528\uff0c\u8003\u9a6c\u65af\u4eae\u84dd\u7684\u6700\u5927\u5438\u6536\u5cf0\u4f1a\u4ece\uff08Metachromazy \u53cd\u5e94\uff09465 nm\u53d8\u4e3a595 nm\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-46572 size-full\" src=\"https:\/\/assets.www.gsbet888.com\/uploads\/2023\/08\/d1951288459d97eb762ea5853d796d8e.png\" alt=\"\" width=\"686\" height=\"430\" srcset=\"https:\/\/assets.www.gsbet888.com\/uploads\/2023\/08\/d1951288459d97eb762ea5853d796d8e.png 686w, https:\/\/assets.www.gsbet888.com\/uploads\/2023\/08\/d1951288459d97eb762ea5853d796d8e-300x188.png 300w, https:\/\/assets.www.gsbet888.com\/uploads\/2023\/08\/d1951288459d97eb762ea5853d796d8e-680x426.png 680w\" sizes=\"(max-width: 686px) 100vw, 686px\" \/><\/p>\n<h4><strong><b>\u4f18\u70b9<\/b><\/strong><\/h4>\n<ul>\n<li>\u6d4b\u5b9a\u8303\u56f4\u572801-2 mg\/mL\u3001\u7075\u654f\u5ea6\u9ad8<\/li>\n<li>\u64cd\u4f5c\u5341\u5206\u7b80\u5355\u8fc5\u901f<\/li>\n<li>\u53d7\u87af\u5408\u5242\u548c\u8fd8\u539f\u5242\u7684\u5f71\u54cd\u5f88\u5c0f<\/li>\n<\/ul>\n<h4><strong><b>\u7f3a\u70b9<\/b><\/strong><\/h4>\n<ul>\n<li>\u6613\u53d7\u754c\u9762\u6d3b\u6027\u5242\u7684\u5f71\u54cd<\/li>\n<li>\u56e0\u4e3a\u53cd\u5e94\u6db2\u662f\u9178\u6027\u7684\uff0c\u542b\u6709\u8102\u8d28\u7684\u6837\u54c1\u5bb9\u6613\u4ea7\u751f\u6c89\u6dc0<\/li>\n<li>\u7ed3\u679c\u4f1a\u56e0\u86cb\u767d\u8d28\u79cd\u7c7b\u800c\u53d8\u5316<\/li>\n<li>\u8272\u7d20\u4f1a\u88ab\u5438\u9644\u5230\u73bb\u7483\u6216\u77f3\u82f1\u5236\u7684\u5438\u6536\u6c60\u4e0a<\/li>\n<\/ul>\n<h4><strong><b>\u64cd\u4f5c\u6b65\u9aa4<\/b><\/strong><\/h4>\n<p>Bradford\u8bd5\u5242\u5404\u4e2a\u8bd5\u5242\u516c\u53f8\u90fd\u6709\u552e\u5356\uff0c\u4f46\u662f\u6309\u7167\u4e0b\u9762\u7684\u65b9\u6cd5\u4e5f\u53ef\u4ee5\u81ea\u5236\u3002<\/p>\n<p><strong><b>Bradford<\/b><\/strong><strong><b>\u8bd5\u5242\u7684\u8c03\u5236\u6cd5<\/b><\/strong><\/p>\n<p>\u5c06\u8003\u9a6c\u65af\u4eae\u84ddCBB G-250 (200 mg)\u52a0\u5165\u4e59\u9187\uff08100 mL\uff09\u4e2d\u6405\u62cc\u5747\u5300\u4f7f\u5176\u5145\u5206\u6eb6\u89e3\u3002\u7136\u540e\u52a0\u516585%\u7684\u78f7\u9178\uff08200 mL\uff09\u3002\u7136\u540e\u7528\u8d85\u7eaf\u6c34\u5b9a\u5bb9\u81f32L\u3002\u6eb6\u6db2\u4e2d\u542b\u6709\u7684\u84dd\u8272\u4e0d\u6eb6\u7269\u7528\u8131\u8102\u68c9\u6216\u6ee4\u7eb8\u8fc7\u6ee4\u3002\u8bd5\u5242\u653e\u5165\u51b0\u7bb1\u5185\u906e\u5149\u4fdd\u5b58\u3002<\/p>\n<p><strong><b>\u6b65\u9aa4<\/b><\/strong><\/p>\n<ol>\n<li>\u5411100\u03bcL\u6837\u54c1\u4e2d\u52a0\u51651ml\u7684Bradford\u8bd5\u5242\uff0c\u7528\u9ad8\u901f\u6405\u62cc\u5668\u6405\u62cc\u5747\u5300\u3002<\/li>\n<li>5\uff5e30\u5206\u949f\u5185\u6d4b\u91cf\u6df7\u5408\u6eb6\u6db2\u5728595 nm\u5904\u7684\u5438\u5149\u5ea6\u3002<\/li>\n<li>\u4ee5BSA\uff08\u725b\u8840\u6e05\u767d\u86cb\u767d\uff09\u4f5c\u4e3a\u6807\u51c6\u54c1\u5236\u4f5c\u7a00\u91ca\u66f2\u7ebf\u3002<\/li>\n<\/ol>\n<p>\u5982\u679c\u5f85\u6d4b\u6837\u54c1\u662f\u819c\u86cb\u767d\u8d28\u7684\u8bdd\uff0c\u5efa\u8bae\u52a0\u51651M NaOH\u6c34\u6eb6\u6db2\u4ee5\u65b9\u4fbf\u6eb6\u89e3<sub>[2]<\/sub>\u3002<\/p>\n<h4><strong><b>\u53c2\u8003\u6587\u732e<\/b><\/strong><\/h4>\n<ol>\n<li>\u201d\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5b9a\u91cf\u6cd5\u201d\u3000\u9234\u6728\u7965\u592b\u3001\u3076\u3093\u305b\u304d<strong><b>2018<\/b><\/strong>,\u00a01, 2.\u00a0<a href=\"https:\/\/www.jsac.or.jp\/bunseki\/pdf\/bunseki2018\/201801nyuumon.pdf\">[PDF]<\/a><\/li>\n<li>\u201c[6] Quantification of protein\u201d Stoscheck, C. M.\u00a0 Enzymol.<strong><b>1990<\/b><\/strong>,\u00a0182, 50. doi:<a href=\"https:\/\/doi.org\/10.1016\/0076-6879(90)82008-P\">10.1016\/0076-6879(90)82008-P<\/a><\/li>\n<li>\u201cComparison of five methods for determination fo total plasma protein concentration\u201d Okutucu, B.; Dincer, A.; Habib, \u00d6.; Zihnioglu, F. J.\u00a0 Biophys. Methods<strong><b>2007<\/b><\/strong>,\u00a070, 709. doi:<a href=\"https:\/\/doi.org\/10.1016\/j.jbbm.2007.05.009\">10.1016\/j.jbbm.2007.05.009<\/a><\/li>\n<li>\u201cA Rapid and Sensitive Method for the Quantification of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding\u201d Bradford, M. M.\u00a0 Biochem.\u00a0<strong><b>1976<\/b><\/strong>,\u00a072 (1\u20132), 248\u2013254. doi:<a href=\"https:\/\/doi.org\/10.1006\/abio.1976.9999\">10.1006\/abio.1976.9999<\/a><\/li>\n<li>\u201dLinearization of the Bradford Protein Assay Increases Its Sensitivity: Theoretical and Experimental Studies\u201d Zor, T.; Selinger, Z.\u00a0Anal Biochem.<strong><b>1996<\/b><\/strong>,\u00a0236, 302. doi:<a href=\"https:\/\/doi.org\/10.1006\/abio.1996.0171\">1006\/abio.1996.0171<\/a><\/li>\n<\/ol>\n<p>\u672c\u6587\u7248\u6743\u5c5e\u4e8e\u00a0Chem-Station\u5316\u5b66\u7a7a\u95f4\uff0c \u6b22\u8fce\u70b9\u51fb\u6309\u94ae\u5206\u4eab\uff0c\u672a\u7ecf\u8bb8\u53ef\uff0c\u8c22\u7edd\u8f6c\u8f7d<\/p>\n","protected":false},"excerpt":{"rendered":"\u8bd1\u81eaChem-Station\u7f51\u7ad9\u65e5\u672c\u7248\u00a0\u539f\u6587\u94fe\u63a5\uff1a\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5b9a\u91cf\u6cd5\u2015\u30d6\u30e9\u30c3\u30c9\u30d5\u30a9\u30fc\u30c9\u6cd5 Protein Quantification \u2013 Bradford Protein Assay \u7ffb\u8bd1\uff1a\u70b8\u9e21 \u539f\u7406 \u5e03\u62c9\u5fb7\u798f\u86cb\u767d\u8d28\u5b9a\u91cf\u6cd5\uff08Bradford Protein Assary\uff09\u4e0eBCA\u6cd5\u5e76\u5217\u4e3a\u6700\u5e38\u4f7f\u7528\u7684\u86cb\u767d\u8d28\u6d53\u5ea6\u5b9a\u91cf\u6cd5\u3002 \u539f\u7406\u662f\u8003\u9a6c\u65af\u4eae\u84dd\uff08CBB\uff09 G-250\u8fd9\u79cd\u8272\u7d20\u4f1a\u4e0e\u86cb\u767d\u8d28\u7684\u78b1\u6027\u4e14\u5c5e\u4e8e\u82b3\u9999\u65cf\u7684\u6c28\u57fa\u9178\u7684\u4fa7\u94fe\u76f8\u4e92\u4f5c\u7528\uff0c\u8003\u9a6c\u65af\u4eae\u84dd\u7684\u6700\u5927\u5438\u6536\u5cf0\u4f1a\u4ece\uff08Metachromazy \u53cd\u5e94\uff09465 nm\u53d8\u4e3a595 nm\u3002 \u4f18\u70b9 \u6d4b\u5b9a\u8303\u56f4\u572801-2 mg\/mL\u3001\u7075\u654f\u5ea6\u9ad8 \u64cd\u4f5c\u5341\u5206\u7b80\u5355\u8fc5\u901f \u53d7\u87af\u5408\u5242\u548c\u8fd8\u539f\u5242\u7684\u5f71\u54cd\u5f88\u5c0f \u7f3a\u70b9 \u6613\u53d7\u754c\u9762\u6d3b\u6027\u5242\u7684\u5f71\u54cd \u56e0\u4e3a\u53cd\u5e94\u6db2\u662f\u9178\u6027\u7684\uff0c\u542b\u6709\u8102\u8d28\u7684\u6837\u54c1\u5bb9\u6613\u4ea7\u751f\u6c89\u6dc0 \u7ed3\u679c\u4f1a\u56e0\u86cb\u767d\u8d28\u79cd\u7c7b\u800c\u53d8\u5316 \u8272\u7d20\u4f1a\u88ab\u5438\u9644\u5230\u73bb\u7483\u6216\u77f3\u82f1\u5236\u7684\u5438\u6536\u6c60\u4e0a \u64cd\u4f5c\u6b65\u9aa4 Bradford\u8bd5\u5242\u5404\u4e2a\u8bd5\u5242\u516c\u53f8\u90fd\u6709\u552e\u5356\uff0c\u4f46\u662f\u6309\u7167\u4e0b\u9762\u7684\u65b9\u6cd5\u4e5f\u53ef\u4ee5\u81ea\u5236\u3002 Bradford\u8bd5\u5242\u7684\u8c03\u5236\u6cd5 \u5c06\u8003\u9a6c\u65af\u4eae\u84ddCBB G-250 (200 mg)\u52a0\u5165\u4e59\u9187\uff08100 mL\uff09\u4e2d\u6405\u62cc\u5747\u5300\u4f7f\u5176\u5145\u5206\u6eb6\u89e3\u3002\u7136\u540e\u52a0\u516585%\u7684\u78f7\u9178\uff08200 mL\uff09\u3002\u7136\u540e\u7528\u8d85\u7eaf\u6c34\u5b9a\u5bb9\u81f32L\u3002\u6eb6\u6db2\u4e2d\u542b\u6709\u7684\u84dd\u8272\u4e0d\u6eb6\u7269\u7528\u8131\u8102\u68c9\u6216\u6ee4\u7eb8\u8fc7\u6ee4\u3002\u8bd5\u5242\u653e\u5165\u51b0\u7bb1\u5185\u906e\u5149\u4fdd\u5b58\u3002 \u6b65\u9aa4 \u5411100\u03bcL\u6837\u54c1\u4e2d\u52a0\u51651ml\u7684Bradford\u8bd5\u5242\uff0c\u7528\u9ad8\u901f\u6405\u62cc\u5668\u6405\u62cc\u5747\u5300\u3002 5\uff5e30\u5206\u949f\u5185\u6d4b\u91cf\u6df7\u5408\u6eb6\u6db2\u5728595 nm\u5904\u7684\u5438\u5149\u5ea6\u3002 \u4ee5BSA\uff08\u725b\u8840\u6e05\u767d\u86cb\u767d\uff09\u4f5c\u4e3a\u6807\u51c6\u54c1\u5236\u4f5c\u7a00\u91ca\u66f2\u7ebf\u3002 \u5982\u679c\u5f85\u6d4b\u6837\u54c1\u662f\u819c\u86cb\u767d\u8d28\u7684\u8bdd\uff0c\u5efa\u8bae\u52a0\u51651M NaOH\u6c34\u6eb6\u6db2\u4ee5\u65b9\u4fbf\u6eb6\u89e3[2]\u3002 \u53c2\u8003\u6587\u732e \u201d\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5b9a\u91cf\u6cd5\u201d\u3000\u9234\u6728\u7965\u592b\u3001\u3076\u3093\u305b\u304d2018,\u00a01, 2.\u00a0[PDF] \u201c[6] Quantification of protein\u201d Stoscheck, C. M.\u00a0 Enzymol.1990,\u00a0182, 50. doi:10.1016\/0076-6879(90)82008-P \u201cComparison of five methods for determination fo total plasma protein concentration\u201d Okutucu, B.; Dincer, A.; Habib, \u00d6.; Zihnioglu, F. J.\u00a0 Biophys. Methods2007,\u00a070, 709. doi:10.1016\/j.jbbm.2007.05.009 \u201cA Rapid and Sensitive Method for the Quantification of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding\u201d Bradford, M. M.\u00a0 Biochem.\u00a01976,\u00a072 (1\u20132), 248\u2013254. doi:10.1006\/abio.1976.9999 \u201dLinearization of the Bradford [&hellip;]","protected":false},"author":26,"featured_media":46571,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","enabled":false},"version":2}},"categories":[1458],"tags":[228,964,3972],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"https:\/\/assets.www.gsbet888.com\/uploads\/2023\/08\/d2e24fc8a86911d4c6aed2dbf0a32936.png","jetpack_shortlink":"https:\/\/wp.me\/p96op1-c7c","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/posts\/46574"}],"collection":[{"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/users\/26"}],"replies":[{"embeddable":true,"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/comments?post=46574"}],"version-history":[{"count":2,"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/posts\/46574\/revisions"}],"predecessor-version":[{"id":46576,"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/posts\/46574\/revisions\/46576"}],"wp:featuredmedia":[{"embeddable":true,"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/media\/46571"}],"wp:attachment":[{"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/media?parent=46574"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/categories?post=46574"},{"taxonomy":"post_tag","embeddable":true,"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/tags?post=46574"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}