{"id":46862,"date":"2023-09-23T08:00:02","date_gmt":"2023-09-23T00:00:02","guid":{"rendered":"\/\/www.gsbet888.com\/?p=46862"},"modified":"2023-09-20T08:49:28","modified_gmt":"2023-09-20T00:49:28","slug":"%e3%80%8cspotlight-research%e3%80%8d%e9%85%b6%e4%bf%83%e8%bf%9b%e7%be%9f%e8%83%ba%e7%9a%84%e6%b0%ae%e6%8f%92%e5%85%a5%e5%8f%8d%e5%ba%94","status":"publish","type":"post","link":"\/\/www.gsbet888.com\/%e5%8c%96%e5%ad%a6%e6%9d%82%e8%ae%b0\/recentpaper\/%e7%83%ad%e7%82%b9%e7%a0%94%e7%a9%b6\/2023\/09\/%e3%80%8cspotlight-research%e3%80%8d%e9%85%b6%e4%bf%83%e8%bf%9b%e7%be%9f%e8%83%ba%e7%9a%84%e6%b0%ae%e6%8f%92%e5%85%a5%e5%8f%8d%e5%ba%94.html","title":{"rendered":"\u300cSpotlight Research\u300d\u9176\u4fc3\u8fdb\u7f9f\u80fa\u7684\u6c2e\u63d2\u5165\u53cd\u5e94"},"content":{"rendered":"<p><strong>\u4f5c\u8005\uff1a\u77f3\u6cb9\u919a<\/strong><\/p>\n<p>\u672c\u671f\u70ed\u70b9\u7814\u7a76\uff0c\u6211\u4eec\u9080\u8bf7\u5230\u4e86\u672c\u6587\u7b2c\u4e00\u4f5c\u8005\u6765\u81ea\u52a0\u5dde\u7406\u5de5\u5b66\u9662\u7684Gao Shilong\u535a\u58eb\u4e3a\u6211\u4eec\u5206\u4eab\u3002<\/p>\n<p>2023\u5e749\u67086\u65e5\uff0c<em>JACS<\/em>\u5728\u7ebf\u53d1\u8868\u4e86\u6765\u81ea\u52a0\u5dde\u7406\u5de5\u5b66\u9662Frances H. Arnold\u6559\u6388\u56e2\u961f\u9898\u4e3a\u300cEnzymatic Nitrogen Incorporation Using Hydroxylamine\u300d\u7684\u7814\u7a76\u8bba\u6587\u3002\u672c\u6587\u4e2d\uff0c\u4f5c\u8005\u53d1\u73b0\u4e86\u4e00\u79cd\u8840\u7ea2\u7d20\u9176ParPgb-HYA-5209\uff0c\u5176\u53ef\u5229\u7528NH<sub>2<\/sub>OH\u4f5c\u4e3a\u6c28\u6e90\uff0c\u6210\u529f\u5b9e\u73b0\u4e86\u82c4\u57faC-H\u80fa\u5316\u548c\u82ef\u4e59\u70ef\u6c28\u57fa\u7f9f\u57fa\u5316\u53cd\u5e94\u3002\u673a\u7406\u7814\u7a76\u63ed\u793a\u4e86\u4e00\u4e2a\u9010\u6b65\u7684\u81ea\u7531\u57fa\u53cd\u5e94\u9014\u5f84\uff0c\u5176\u4e2dC-H\u952e\u65ad\u88c2\u662f\u51b3\u5b9a\u901f\u7387\u7684\u6b65\u9aa4\u3002\u672c\u6587\u4e3a\u5236\u5907\u542b\u6c2e\u5206\u5b50\u7684\u53ef\u6301\u7eed\u7ecf\u6d4e\u7684\u751f\u7269\u50ac\u5316\u9014\u5f84\u5f00\u8f9f\u4e86\u65b0\u7684\u9053\u8def\u3002\u6b64\u5916\uff0c\u4f5c\u8005\u63d0\u51fa\u4e86\u4e00\u79cd\u65b0\u7684\u53ef\u80fd\u6027\uff0c\u5373\u53d1\u73b0\u5229\u7528NH<sub>2<\/sub>OH\u4f5c\u4e3a\u80fa\u6e90\u7684\u5929\u7136\u9176\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-46864 size-full\" src=\"https:\/\/assets.www.gsbet888.com\/uploads\/2023\/09\/image002-5.png\" alt=\"\" width=\"604\" height=\"239\" srcset=\"https:\/\/assets.www.gsbet888.com\/uploads\/2023\/09\/image002-5.png 604w, https:\/\/assets.www.gsbet888.com\/uploads\/2023\/09\/image002-5-300x119.png 300w\" sizes=\"(max-width: 604px) 100vw, 604px\" \/><\/p>\n<blockquote><p>\u201cEnzymatic Nitrogen Incorporation Using Hydroxylamine.<\/p>\n<p>Shilong Gao, Anuvab Das, Edwin Alfonzo, Kathleen M. Sicinski, Dominic Rieger, and Frances H. Arnold* <em>J.<\/em> <em>Am. Chem. Soc.,<\/em><strong>2023<\/strong>,<em> ASAP<\/em>. Doi: <a href=\"https:\/\/pubs.acs.org\/doi\/full\/10.1021\/jacs.3c08053\">10.1021\/jacs.3c08053 <\/a>\u201d<\/p><\/blockquote>\n<h4><strong>Q1. <\/strong><strong>\u8bf7\u5bf9\u201c<\/strong><strong>Enzymatic Nitrogen Incorporation Using Hydroxylamine<\/strong><strong>\u201d<\/strong><strong>\u4f5c<\/strong><strong>\u4e00\u4e2a\u7b80\u5355\u4ecb\u7ecd\u3002<\/strong><\/h4>\n<p>\u6b64\u5de5\u4f5c\u63a2\u8ba8\u4e86\u5c06\u7f9f\u80fa\uff08NH<sub>2<\/sub>OH\uff09\u7528\u4f5c\u9176\u50ac\u5316\u6c2e\u5f15\u5165\u7684\u539f\u6599\u7684\u53ef\u80fd\u6027\uff0c\u8fd9\u4e2a\u8fc7\u7a0b\u5bf9\u4e8e\u751f\u7269\u50ac\u5316\u548c\u53ef\u6301\u7eed\u5316\u5b66\u6709\u7740\u91cd\u8981\u7684\u5f71\u54cd\u3002\u8be5\u7814\u7a76\u53d7\u81ea\u7136\u754c\u5bf9\u751f\u7269\u5206\u5b50\u529f\u80fd\u5316\u7684\u542f\u53d1\uff0c\u7814\u7a76\u4e86\u4f9d\u8d56\u8840\u7ea2\u7d20\u7684\u9176\u4ee5\u53ca\u5b83\u4eec\u5904\u7406NH<sub>2<\/sub>OH\u8fdb\u884c\u80fa\u5316\u53cd\u5e94\u7684\u6f5c\u529b\u3002\u7f9f\u80fa\u5728\u5176\u5316\u5b66\u6027\u8d28\u4e0a\u4e0e\u8fc7\u6c27\u5316\u6c22\uff08H<sub>2<\/sub>O<sub>2<\/sub>\uff09\u6709\u4e00\u4e9b\u76f8\u4f3c\u4e4b\u5904\uff0c\u8fd9\u8868\u660e\u5b83\u53ef\u80fd\u901a\u8fc7\u9176\u50ac\u5316\u6765\u5b9e\u73b0\u6c2e\u5f15\u5165\u3002\u5c3d\u7ba1\u7f9f\u80fa\u5728\u571f\u58e4\u5fae\u751f\u7269\u4e2d\u81ea\u7136\u5b58\u5728\uff0c\u4f46\u4ee5\u5f80\u5e76\u672a\u88ab\u77e5\u9053\u5b83\u53ef\u4ee5\u7528\u4f5c\u751f\u7269\u5408\u6210\u4e2d\u7684\u80fa\u6e90\u3002\u8bba\u6587\u4ecb\u7ecd\u4e86\u4e00\u79cd\u7ecf\u8fc7\u6539\u826f\u7684\u8840\u7ea2\u7d20\u9176ParPgb-HYA-5209\u7684\u53d1\u73b0\uff0c\u5b83\u80fd\u591f\u5229\u7528NH<sub>2<\/sub>OH\u8fdb\u884c\u82c4\u57faC-H\u4e3b\u80fa\u5316\u548c\u82ef\u4e59\u70ef\u6c28\u57fa\u7f9f\u57fa\u5316\u53cd\u5e94\u3002\u5b9a\u5411\u8fdb\u5316\u8fdb\u4e00\u6b65\u589e\u5f3a\u4e86\u9176\u7684\u6d3b\u6027\u3002\u673a\u7406\u7814\u7a76\u63ed\u793a\u4e86\u4e00\u4e2a\u9010\u6b65\u7684\u81ea\u7531\u57fa\u53cd\u5e94\u9014\u5f84\uff0c\u5176\u4e2dC-H\u952e\u65ad\u88c2\u662f\u51b3\u5b9a\u901f\u7387\u7684\u6b65\u9aa4\u3002\u8fd9\u7bc7\u8bba\u6587\u7684\u53d1\u73b0\u4e3a\u5236\u5907\u542b\u6c2e\u5206\u5b50\u7684\u53ef\u6301\u7eed\u7ecf\u6d4e\u7684\u751f\u7269\u50ac\u5316\u9014\u5f84\u5f00\u8f9f\u4e86\u65b0\u7684\u9053\u8def\u3002\u6b64\u5916\uff0c\u6211\u4eec\u63d0\u51fa\u4e86\u4e00\u79cd\u65b0\u7684\u53ef\u80fd\u6027\uff0c\u5373\u53d1\u73b0\u5229\u7528NH<sub>2<\/sub>OH\u4f5c\u4e3a\u80fa\u6e90\u7684\u5929\u7136\u9176\u3002<\/p>\n<h4><strong>Q2. <\/strong><strong>\u6709\u5173\u672c\u6b21\u7814\u7a76\u7684\u65f6\u5019\u9047\u5230\u8fc7\u600e\u6837\u7684\u56f0\u96be\u5462\uff1f\u53c8\u662f\u600e\u6837\u514b\u670d\u7684\u5462<\/strong><\/h4>\n<p>NH<sub>2<\/sub>OH\u7684\u53cd\u5e94\u6027\uff1a NH<sub>2<\/sub>OH\u76f8\u5bf9\u4e8e\u4eb2\u7535\u6027\u7f9f\u80fa\u884d\u751f\u8bd5\u5242\u7f3a\u4e4f\u5438\u7535\u5b50\u57fa\u56e2\uff0c\u5bfc\u81f4N-O\u952e\u89e3\u79bb\u80fd\u5347\u9ad8\u3002\u8fd9\u5728\u5bfb\u627e\u80fd\u591f\u6709\u6548\u6fc0\u6d3bNH2OH\u8fdb\u884c\u6c2e\u5f15\u5165\u7684\u9176\u65f6\u6784\u6210\u4e86\u4e00\u9053\u969c\u788d\u3002\u4e3a\u4e86\u6210\u529f\u8fdb\u884c\u9176\u50ac\u5316\uff0c\u5173\u952e\u662f\u8840\u7ea2\u7d20\u9176\u5fc5\u987b\u9009\u62e9\u6027\u5730\u7ed3\u5408NH<sub>2<\/sub>OH\u4e2d\u7684\u6c2e\uff0c\u800c\u4e0d\u662f\u6c27\uff0c\u4ee5\u5f62\u6210\u53cd\u5e94\u4e2d\u95f4\u4f53\u3002\u5b9e\u73b0\u8fd9\u79cd\u9009\u62e9\u6027\u9700\u8981\u4ed4\u7ec6\u8003\u8651\u9176\u7684\u6d3b\u6027\u4f4d\u70b9\u3002<\/p>\n<p>\u5728\u7814\u7a76\u7684\u521d\u671f\u9636\u6bb5\uff0c\u51e0\u79cd\u8840\u7ea2\u7d20\u9176\u53d8\u4f53\u4ec5\u8868\u73b0\u51fa\u5fae\u91cf\u4ea7\u7269\u5f62\u6210\u7684\u6c34\u5e73\u3002\u8fd9\u79cd\u6709\u9650\u7684\u53cd\u5e94\u6027\u5bf9\u4e8e\u786e\u5b9a\u8fdb\u4e00\u6b65\u7814\u7a76\u7684\u6709\u5e0c\u671b\u7684\u5019\u9009\u8005\u6784\u6210\u4e86\u91cd\u5927\u6311\u6218\u3002\u5f97\u76ca\u4e8eParPgb\u7684\u9ad8\u7a33\u5b9a\u6027\uff0c\u5b9a\u5411\u8fdb\u5316\u5f88\u5feb\u63d0\u9ad8\u4e86\u9176\u7684\u53cd\u5e94\u6027\u3002<\/p>\n<p>\u4f18\u5316\u53cd\u5e94\u6761\u4ef6\uff1a\u5927\u80a0\u6746\u83cc\u4e2d\u86cb\u767d\u8d28\u8868\u8fbe\u548c\u8840\u7ea2\u7d20\u4ea7\u751f\u4e4b\u95f4\u7684\u5dee\u5f02\u9020\u6210\u8840\u7ea2\u7d20\u5360\u4f4d\u4ec5\u7ea6\u4e3a25%\u3002\u6211\u4eec\u53d1\u73b0\u5728\u86cb\u767d\u8d28\u7eaf\u5316\u8fc7\u7a0b\u4e2d\uff0c\u4f7f\u7528\u5916\u6e90\u7684\u8840\u7ea2\u7d20b\u8f85\u56e0\u5b50\u80fd\u91cd\u6784\u5b8c\u6574\u7684\u9176\u3002\u6700\u540e\uff0c\u6211\u4eec\u53d1\u73b0\u8fd8\u539f\u6761\u4ef6\u663e\u8457\u63d0\u9ad8\u4e86\u7eaf\u5316\u9176\u7684\u53cd\u5e94\u6027\u3002<\/p>\n<p>\u7406\u89e3\u673a\u5236\uff1a\u89e3\u5f00\u5229\u7528NH<sub>2<\/sub>OH\u8fdb\u884c\u82c4\u57faC-H\u4e3b\u80fa\u5316\u7684\u590d\u6742\u673a\u5236\u662f\u4e00\u9879\u6311\u6218\u3002\u8fd9\u9700\u8981\u8fdb\u884c\u52a8\u529b\u5b66\u540c\u4f4d\u7d20\u6548\u5e94\uff08KIE\uff09\u5b9e\u9a8c\u548c\u81ea\u7531\u57fa\u63a2\u9488\u7814\u7a76\uff0c\u8fd9\u4e9b\u7814\u7a76\u63d0\u4f9b\u4e86\u6709\u4ef7\u503c\u7684\u89c1\u89e3\uff0c\u4f46\u4e5f\u589e\u52a0\u4e86\u89e3\u91ca\u4e0a\u7684\u590d\u6742\u6027\u3002\u672a\u6765\u5bf9\u673a\u7406\u7684\u7814\u7a76\u5de5\u4f5c\u4f1a\u8fdb\u4e00\u6b65\u63ed\u793a\u53cd\u5e94\u8fc7\u7a0b\u4e2d\u53ef\u80fd\u6d89\u53ca\u7684\u4e2d\u95f4\u4f53\u3002<\/p>\n<h4><strong>Q3. <\/strong><strong>\u672c\u6b21\u7814\u7a76\u4e3b\u4f53\uff0c\u6709\u6ca1\u6709\u4ec0\u4e48\u8ba9\u60a8\u611f\u89c9\u7279\u522b\u8f9b\u82e6\u548c\u70e7\u8111\u5462\uff1f<\/strong><\/h4>\n<p>\u5728\u9009\u62e9\u8fdb\u5316\u7684\u8d77\u59cb\u70b9\u65f6\uff0c\u6211\u4eec\u523b\u610f\u9009\u62e9\u4e86\u53cd\u5e94\u6027\u8f83\u4f4e\u3001\u4f46\u4f53\u79ef\u5c0f\u7a33\u5b9a\u6027\u9ad8\u7684\u9176\u3002\u8fd9\u4e00\u7279\u6027\u4f7f\u5176\u80fd\u591f\u5bb9\u5fcd\u6709\u76ca\u4f46\u901a\u5e38\u4f1a\u4f7f\u5176\u4e0d\u7a33\u5b9a\u7684\u7a81\u53d8\u3002\u5f97\u76ca\u4e8e\u6b64\uff0c\u5728\u7b2c\u4e00\u8f6e\u5b9a\u5411\u8fdb\u5316\u4e2d\uff0c\u6211\u4eec\u53d1\u73b0\u4e86\u5927\u91cf\u6709\u76ca\u7684\u7a81\u53d8\u3002\u4f46\u4e0e\u4e4b\u4f34\u968f\u7684\u6311\u6218\u5728\u4e8e\u5982\u4f55\u9ad8\u6548\u7684\u91cd\u65b0\u7ec4\u5408\u8fd9\u4e9b\u7a81\u53d8\u3002\u6700\u7ec8\uff0c\u6211\u4eec\u91c7\u7528\u4e86Stagger Extension Process\u7684\u7b56\u7565\u3002\u5728\u4e0d\u52a0\u5165\u65b0\u7a81\u53d8\u7684\u524d\u63d0\u4e0b\uff0c\u4e09\u8f6e\u5b9a\u5411\u8fdb\u5316\u6210\u529f\u5730\u4ece\u521d\u59cb\u768441\u4e2a\u7a81\u53d8\u4e2d\u91cd\u6392\u7b5b\u9009\u51fa\u4e94\u4e2a\u6709\u76ca\u7684\u7a81\u53d8\uff0c\u9ad8\u6548\u5730\u63d0\u9ad8\u4e86\u9176\u7684\u53cd\u5e94\u6027\u3002<\/p>\n<p>\u5c06\u590d\u6742\u7684\u79d1\u5b66\u6982\u5ff5\u7ffb\u8bd1\u6210\u4e00\u7bc7\u8fde\u8d2f\u4e14\u5bb9\u6613\u7406\u89e3\u7684\u53d9\u8ff0\u662f\u4e00\u9879\u6311\u6218\u3002\u7814\u7a76\u6d89\u53ca\u590d\u6742\u7684\u9176\u673a\u5236\u3001\u81ea\u7531\u57fa\u9014\u5f84\u548c\u52a8\u529b\u5b66\u53c2\u6570\uff0c\u8fd9\u4e9b\u5bf9\u975e\u4e13\u4e1a\u8bfb\u8005\u6765\u8bf4\u53ef\u80fd\u4e0d\u5bb9\u6613\u7406\u89e3\u3002\u5728\u79d1\u5b66\u51c6\u786e\u6027\u548c\u53ef\u8bfb\u6027\u4e4b\u95f4\u4fdd\u6301\u5e73\u8861\u3001\u5728\u63d0\u4f9b\u8db3\u591f\u7684\u7ec6\u8282\u4ee5\u4f20\u8fbe\u7814\u7a76\u91cd\u8981\u6027\u7684\u540c\u65f6\u4fdd\u6301\u7b80\u6d01\u4e5f\u662f\u4e00\u9879\u5fae\u5999\u7684\u4efb\u52a1\u3002\u5728\u5199\u8fd9\u7bc7\u8bba\u6587\u65f6\uff0c\u6211\u4eec\u5e0c\u671b\u8bfb\u8005\u80fd\u8bb0\u4f4f\u7684\u662f\u9176\u5229\u7528NH<sub>2<\/sub>OH\u4f5c\u4e3a\u80fa\u6e90\u7684\u53ef\u80fd\u6027\u3002\u6211\u4eec\u5e0c\u671b\u770b\u5230\u66f4\u591a\u5bf9NH<sub>2<\/sub>OH\u5728\u81ea\u7136\u754c\u4e2d\u4f5c\u7528\u7684\u63a2\u7d22\u3002<\/p>\n<h4><strong>Q4. <\/strong><strong>\u5c06\u6765\u60f3\u7ee7\u7eed\u7814\u7a76\u5316\u5b66\u7684\u54ea\u4e2a\u65b9\u5411\u5462\uff1f<\/strong><\/h4>\n<p>\u5e0c\u671b\u66f4\u6df1\u5165\u5730\u7814\u7a76\u86cb\u767d\u8d28\u5de5\u7a0b\uff0c\u65f6\u523b\u5173\u6ce8\u8be5\u9886\u57df\u7684\u6700\u65b0\u53d1\u5c55\uff0c\u4f8b\u5982\u8ba1\u7b97\u86cb\u767d\u8d28\u8bbe\u8ba1\u3001\u5b9a\u5411\u8fdb\u5316\u7b56\u7565\u548c\u9ad8\u901a\u91cf\u7b5b\u9009\u65b9\u6cd5\u3002\u8fd9\u4e9b\u5de5\u5177\u53ef\u4ee5\u6709\u52a9\u4e8e\u6709\u9488\u5bf9\u6027\u5730\u8bbe\u8ba1\u548c\u4f18\u5316\u9176\uff0c\u4ee5\u7528\u4e8e\u7279\u5b9a\u7684\u5e94\u7528\u3002\u63a2\u7d22\u5728\u57fa\u56e0\u7f16\u8f91\u9886\u57df\u4e2d\u786e\u5b9a\u5de5\u7a0b\u9176\u7684\u5177\u4f53\u5e94\u7528\u3002\u4f8b\u5982\uff0c\u63a2\u7d22\u5de5\u7a0b\u9176\u5982\u4f55\u63d0\u9ad8\u57fa\u56e0\u7f16\u8f91\u6280\u672f\uff08\u5982CRISPR-Cas9\uff09\u7684\u7cbe\u786e\u6027\u3001\u6548\u7387\u548c\u5b89\u5168\u6027\u3002\u7814\u7a76\u5728\u57fa\u56e0\u6cbb\u7597\u548c\u7cbe\u51c6\u533b\u5b66\u4e2d\u7684\u6f5c\u5728\u6cbb\u7597\u5e94\u7528\u3002\u5e0c\u671b\u4e0e\u5206\u5b50\u751f\u7269\u5b66\u3001\u9057\u4f20\u5b66\u548c\u751f\u7269\u6280\u672f\u7b49\u76f8\u5173\u9886\u57df\u7684\u4e13\u5bb6\u8fdb\u884c\u5408\u4f5c\u3002\u57fa\u56e0\u7f16\u8f91\u662f\u4e00\u4e2a\u8de8\u5b66\u79d1\u7684\u5de5\u4f5c\uff0c\u4e0e\u4e13\u5bb6\u5408\u4f5c\u53ef\u4ee5\u63d0\u4f9b\u5b9d\u8d35\u7684\u89c1\u89e3\uff0c\u5e76\u4e3a\u8de8\u5b66\u79d1\u7814\u7a76\u63d0\u4f9b\u673a\u4f1a\u3002<\/p>\n<h4><strong>Q5. <\/strong><strong>\u6700\u540e\uff0c\u6709\u4ec0\u4e48\u60f3\u5bf9\u5404\u4f4d\u8bfb\u8005\u8bf4\u7684\u5417\uff1f<\/strong><\/h4>\n<p>\u6b22\u8fce\u66f4\u591a\u5316\u5b66\u9886\u57df\u7684\u7814\u7a76\u8005\u52a0\u5165\u9176\u5de5\u7a0b\u5b66\u7684\u5927\u5bb6\u5ead\u3002<\/p>\n<h4><strong>\u4f5c\u8005\u6559\u80b2\u80cc\u666f\u7b80\u4ecb<\/strong><\/h4>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-46865 size-full\" src=\"https:\/\/assets.www.gsbet888.com\/uploads\/2023\/09\/image003-5.jpg\" alt=\"\" width=\"175\" height=\"262\" \/><\/p>\n<p><strong>\u6559\u80b2\u80cc\u666f\uff1a<\/strong><\/p>\n<p>2008-2014\u4eba\u5927\u9644\u4e2d<\/p>\n<p>2014-2018\u6ce2\u58eb\u987f\u5927\u5b66<\/p>\n<p>2018-2023\u52a0\u5dde\u7406\u5de5<\/p>\n<p>\u672c\u6587\u7248\u6743\u5c5e\u4e8e\u00a0Chem-Station\u5316\u5b66\u7a7a\u95f4\uff0c \u6b22\u8fce\u70b9\u51fb\u6309\u94ae\u5206\u4eab\uff0c\u672a\u7ecf\u8bb8\u53ef\uff0c\u8c22\u7edd\u8f6c\u8f7d.<\/p>\n","protected":false},"excerpt":{"rendered":"\u4f5c\u8005\uff1a\u77f3\u6cb9\u919a \u672c\u671f\u70ed\u70b9\u7814\u7a76\uff0c\u6211\u4eec\u9080\u8bf7\u5230\u4e86\u672c\u6587\u7b2c\u4e00\u4f5c\u8005\u6765\u81ea\u52a0\u5dde\u7406\u5de5\u5b66\u9662\u7684Gao Shilong\u535a\u58eb\u4e3a\u6211\u4eec\u5206\u4eab\u3002 2023\u5e749\u67086\u65e5\uff0cJACS\u5728\u7ebf\u53d1\u8868\u4e86\u6765\u81ea\u52a0\u5dde\u7406\u5de5\u5b66\u9662Frances H. Arnold\u6559\u6388\u56e2\u961f\u9898\u4e3a\u300cEnzymatic Nitrogen Incorporation Using Hydroxylamine\u300d\u7684\u7814\u7a76\u8bba\u6587\u3002\u672c\u6587\u4e2d\uff0c\u4f5c\u8005\u53d1\u73b0\u4e86\u4e00\u79cd\u8840\u7ea2\u7d20\u9176ParPgb-HYA-5209\uff0c\u5176\u53ef\u5229\u7528NH2OH\u4f5c\u4e3a\u6c28\u6e90\uff0c\u6210\u529f\u5b9e\u73b0\u4e86\u82c4\u57faC-H\u80fa\u5316\u548c\u82ef\u4e59\u70ef\u6c28\u57fa\u7f9f\u57fa\u5316\u53cd\u5e94\u3002\u673a\u7406\u7814\u7a76\u63ed\u793a\u4e86\u4e00\u4e2a\u9010\u6b65\u7684\u81ea\u7531\u57fa\u53cd\u5e94\u9014\u5f84\uff0c\u5176\u4e2dC-H\u952e\u65ad\u88c2\u662f\u51b3\u5b9a\u901f\u7387\u7684\u6b65\u9aa4\u3002\u672c\u6587\u4e3a\u5236\u5907\u542b\u6c2e\u5206\u5b50\u7684\u53ef\u6301\u7eed\u7ecf\u6d4e\u7684\u751f\u7269\u50ac\u5316\u9014\u5f84\u5f00\u8f9f\u4e86\u65b0\u7684\u9053\u8def\u3002\u6b64\u5916\uff0c\u4f5c\u8005\u63d0\u51fa\u4e86\u4e00\u79cd\u65b0\u7684\u53ef\u80fd\u6027\uff0c\u5373\u53d1\u73b0\u5229\u7528NH2OH\u4f5c\u4e3a\u80fa\u6e90\u7684\u5929\u7136\u9176\u3002 \u201cEnzymatic Nitrogen Incorporation Using Hydroxylamine. Shilong Gao, Anuvab Das, Edwin Alfonzo, Kathleen M. Sicinski, Dominic Rieger, and Frances H. Arnold* J. Am. Chem. Soc.,2023, ASAP. Doi: 10.1021\/jacs.3c08053 \u201d Q1. \u8bf7\u5bf9\u201cEnzymatic Nitrogen Incorporation Using Hydroxylamine\u201d\u4f5c\u4e00\u4e2a\u7b80\u5355\u4ecb\u7ecd\u3002 \u6b64\u5de5\u4f5c\u63a2\u8ba8\u4e86\u5c06\u7f9f\u80fa\uff08NH2OH\uff09\u7528\u4f5c\u9176\u50ac\u5316\u6c2e\u5f15\u5165\u7684\u539f\u6599\u7684\u53ef\u80fd\u6027\uff0c\u8fd9\u4e2a\u8fc7\u7a0b\u5bf9\u4e8e\u751f\u7269\u50ac\u5316\u548c\u53ef\u6301\u7eed\u5316\u5b66\u6709\u7740\u91cd\u8981\u7684\u5f71\u54cd\u3002\u8be5\u7814\u7a76\u53d7\u81ea\u7136\u754c\u5bf9\u751f\u7269\u5206\u5b50\u529f\u80fd\u5316\u7684\u542f\u53d1\uff0c\u7814\u7a76\u4e86\u4f9d\u8d56\u8840\u7ea2\u7d20\u7684\u9176\u4ee5\u53ca\u5b83\u4eec\u5904\u7406NH2OH\u8fdb\u884c\u80fa\u5316\u53cd\u5e94\u7684\u6f5c\u529b\u3002\u7f9f\u80fa\u5728\u5176\u5316\u5b66\u6027\u8d28\u4e0a\u4e0e\u8fc7\u6c27\u5316\u6c22\uff08H2O2\uff09\u6709\u4e00\u4e9b\u76f8\u4f3c\u4e4b\u5904\uff0c\u8fd9\u8868\u660e\u5b83\u53ef\u80fd\u901a\u8fc7\u9176\u50ac\u5316\u6765\u5b9e\u73b0\u6c2e\u5f15\u5165\u3002\u5c3d\u7ba1\u7f9f\u80fa\u5728\u571f\u58e4\u5fae\u751f\u7269\u4e2d\u81ea\u7136\u5b58\u5728\uff0c\u4f46\u4ee5\u5f80\u5e76\u672a\u88ab\u77e5\u9053\u5b83\u53ef\u4ee5\u7528\u4f5c\u751f\u7269\u5408\u6210\u4e2d\u7684\u80fa\u6e90\u3002\u8bba\u6587\u4ecb\u7ecd\u4e86\u4e00\u79cd\u7ecf\u8fc7\u6539\u826f\u7684\u8840\u7ea2\u7d20\u9176ParPgb-HYA-5209\u7684\u53d1\u73b0\uff0c\u5b83\u80fd\u591f\u5229\u7528NH2OH\u8fdb\u884c\u82c4\u57faC-H\u4e3b\u80fa\u5316\u548c\u82ef\u4e59\u70ef\u6c28\u57fa\u7f9f\u57fa\u5316\u53cd\u5e94\u3002\u5b9a\u5411\u8fdb\u5316\u8fdb\u4e00\u6b65\u589e\u5f3a\u4e86\u9176\u7684\u6d3b\u6027\u3002\u673a\u7406\u7814\u7a76\u63ed\u793a\u4e86\u4e00\u4e2a\u9010\u6b65\u7684\u81ea\u7531\u57fa\u53cd\u5e94\u9014\u5f84\uff0c\u5176\u4e2dC-H\u952e\u65ad\u88c2\u662f\u51b3\u5b9a\u901f\u7387\u7684\u6b65\u9aa4\u3002\u8fd9\u7bc7\u8bba\u6587\u7684\u53d1\u73b0\u4e3a\u5236\u5907\u542b\u6c2e\u5206\u5b50\u7684\u53ef\u6301\u7eed\u7ecf\u6d4e\u7684\u751f\u7269\u50ac\u5316\u9014\u5f84\u5f00\u8f9f\u4e86\u65b0\u7684\u9053\u8def\u3002\u6b64\u5916\uff0c\u6211\u4eec\u63d0\u51fa\u4e86\u4e00\u79cd\u65b0\u7684\u53ef\u80fd\u6027\uff0c\u5373\u53d1\u73b0\u5229\u7528NH2OH\u4f5c\u4e3a\u80fa\u6e90\u7684\u5929\u7136\u9176\u3002 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