{"id":49771,"date":"2024-05-15T08:00:15","date_gmt":"2024-05-15T00:00:15","guid":{"rendered":"\/\/www.gsbet888.com\/?p=49771"},"modified":"2024-05-13T13:56:38","modified_gmt":"2024-05-13T05:56:38","slug":"nat-commun-%ef%bc%9a%e5%9f%ba%e4%ba%8e%e5%9b%9b%e9%9d%a2%e4%bd%93%e7%ba%b3%e7%b1%b3%e7%bb%93%e6%9e%84%e7%9a%84ngag%e5%8f%af%e7%a9%bf%e6%88%b4%e5%be%ae%e9%92%88%e5%af%b9%e8%b6%85%e7%97%95%e9%87%8f","status":"publish","type":"post","link":"\/\/www.gsbet888.com\/%e5%8c%96%e5%ad%a6%e6%9d%82%e8%ae%b0\/recentpaper\/2024\/05\/nat-commun-%ef%bc%9a%e5%9f%ba%e4%ba%8e%e5%9b%9b%e9%9d%a2%e4%bd%93%e7%ba%b3%e7%b1%b3%e7%bb%93%e6%9e%84%e7%9a%84ngag%e5%8f%af%e7%a9%bf%e6%88%b4%e5%be%ae%e9%92%88%e5%af%b9%e8%b6%85%e7%97%95%e9%87%8f.html","title":{"rendered":"Nat. Commun.\uff1a\u57fa\u4e8e\u56db\u9762\u4f53\u7eb3\u7c73\u7ed3\u6784\u7684NgAg\u53ef\u7a7f\u6234\u5fae\u9488\u5bf9\u8d85\u75d5\u91cf\u6838\u9178\u7684\u957f\u671f\u76d1\u6d4b"},"content":{"rendered":"<p style=\"text-align: justify;\">\u4f5c\u8005\uff1a\u82cf\u6253\u6c34<\/p>\n<h4 style=\"text-align: justify;\">\u5bfc\u8bfb\uff1a<\/h4>\n<p style=\"text-align: justify;\">\u8fd1\u65e5\uff0c\u590d\u65e6\u5927\u5b66\u65b9\u96ea\u6069\u6559\u6388\u56e2\u961f\u5728<em>Nat. Commun.<\/em>\u4e2d\u53d1\u8868\u8bba\u6587\uff0c\u62a5\u9053\u4e86\u4e00\u79cd\u57fa\u4e8e\u56db\u9762\u4f53\u7eb3\u7c73\u7ed3\u6784\u7684\u55dc\u76d0\u83ccArgonaute\uff08NgAgo\uff09\u6784\u5efa\u7684\u53ef\u7a7f\u6234\u5fae\u9488\u4f20\u611f\u5668\uff0c\u7528\u4e8e\u957f\u671f\u7a33\u5b9a\u76d1\u6d4b\u4f53\u5185\u8d25\u8840\u75c7\u7684\u8d85\u75d5\u91cf\u672a\u6269\u589e\u6838\u9178\u3002<\/p>\n<blockquote><p>Long-Term Monitoring of Ultratrace Nucleic Acids Using Tetrahedral Nanostructure-Based Ngago on Wearable Microneedles.<\/p>\n<p>Yang, B.; Wang, H.; Kong, J.; Fang, X.<em>\u00a0Nat. Commun.<\/em> <strong>2024,<\/strong> 15 (1), 1936. DOI: <a href=\"https:\/\/www.nature.com\/articles\/s41467-024-46215-w#Sec9\">10.1038\/s41467-024-46215-w <\/a><\/p><\/blockquote>\n<h4 style=\"text-align: justify;\">\u6b63\u6587\uff1a<\/h4>\n<p style=\"text-align: justify;\">\u8fd1\u5e74\u6765\uff0c\u57fa\u56e0\u68c0\u6d4b\u6280\u672f\u7684\u53d1\u5c55\u4e3a\u6838\u9178\u8bca\u65ad\u8bc6\u522b\u75be\u75c5\u63d0\u4f9b\u4e86\u5f3a\u5927\u5de5\u5177\u3002\u5176\u4e2dPCR\u3001\u7b49\u6e29\u6838\u9178\u6269\u589e\u548cCRISPR\u6280\u672f\u7ed3\u5408\u7684\u5168\u57fa\u56e0\u7ec4\u6d4b\u5e8f\u53ef\u63d0\u4f9b\u591a\u79cd\u75c5\u7406\u751f\u7406\u4fe1\u606f\uff0c\u4f46\u5927\u591a\u6280\u672f\u5747\u9762\u4e34\u5468\u8f6c\u65f6\u95f4\u957f\u3001\u8bbe\u5907\u7b28\u91cd\u7b49\u95ee\u9898<sup>[1]<\/sup>\u3002\u65b0\u5174\u7684\u53ef\u7a7f\u6234\u6280\u672f\u5728\u4e2a\u6027\u5316\u533b\u7597\u3001\u533b\u7597\u7269\u8054\u7f51\u548c\u5b9e\u65f6\u75be\u75c5\u8bca\u65ad\u4e2d\u63d0\u4f9b\u4e86\u53d8\u9769\u6027\u6280\u672f\uff0c\u53ef\u80fd\u89e3\u51b3\u4f20\u7edf\u6838\u9178\u5206\u5b50\u8bca\u65ad\u5c40\u9650\u3002\u8fd9\u7c7b\u5148\u8fdb\u7684\u53ef\u7a7f\u6234\u8bbe\u5907\u53ef\u4ee5\u76d1\u6d4b\u6df1\u5c42\u7ec4\u7ec7\u3001\u5fc3\u810f\u529f\u80fd\u3001\u6162\u6027\u611f\u67d3\u6027\u4f24\u53e3\u3001\u4f53\u6e29\u8c03\u8282\u548c\u80bf\u7624\u6d88\u9000\uff0c\u4ee5\u53ca\u8840\u7cd6\u3001\u6c34\u5206\u6d41\u5931\u548c\u6c57\u6db2\u7535\u89e3\u8d28\u76d1\u6d4b<sup>[2]<\/sup>\u3002\u53ef\u7a7f\u6234\u8bbe\u5907\u53ef\u80fd\u4e3a\u6301\u7eed\u76d1\u6d4b\u63d0\u4f9b\u65b0\u9014\u5f84\uff0c\u4f46\u5728ICU\u573a\u666f\u4e2d\u4ecd\u5b58\u5728\u5c40\u9650\uff0c\u7279\u522b\u662f\u5728\u76d1\u6d4b\u4f4e\u4e30\u5ea6\u75be\u75c5\u76f8\u5173\u6e38\u79bbDNA\u6216RNA\u751f\u7269\u6807\u5fd7\u7269\u65b9\u9762\u3002<\/p>\n<p style=\"text-align: justify;\">\u57fa\u4e8e\u6b64\uff0c\u590d\u65e6\u5927\u5b66\u65b9\u96ea\u6069\u6559\u6388\u56e2\u961f\u62a5\u544a\u4e86\u57fa\u4e8e\u56db\u9762\u4f53 DNA \u7eb3\u7c73\u7ed3\u6784 \uff08TDN\uff09 \u548c\u539f\u6838 argonaute \u6280\u672f\u7684\u5b8c\u5168\u96c6\u6210\u7684\u53ef\u7a7f\u6234\u7535\u5b50\u8bbe\u5907\uff0c\u5b9e\u65f6\u76d1\u6d4b\u7531 EBV\u3001\u91d1\u9ec4\u8272\u8461\u8404\u7403\u83cc\u548c\u94dc\u7eff\u5047\u5355\u80de\u83cc (staphylococcus aureus, SA) and pseudomonas aeruginosa, PA)\u5f15\u8d77\u7684\u8113\u6bd2\u75c7\u76f8\u5173\u7684cfDNA\u548cRNA\uff0c\u4ece\u800c\u6269\u5927\u4e86\u5728ICU\u60c5\u51b5\u4e0b\u53d1\u75c5\u65f6\u76d1\u6d4b\u8113\u6bd2\u75c7\u7684\u53ef\u80fd\u6027\u3002<\/p>\n<p style=\"text-align: justify;\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-49773 size-full\" src=\"https:\/\/assets.www.gsbet888.com\/uploads\/2024\/05\/image003-4.png\" alt=\"\" width=\"820\" height=\"788\" srcset=\"https:\/\/assets.www.gsbet888.com\/uploads\/2024\/05\/image003-4.png 820w, https:\/\/assets.www.gsbet888.com\/uploads\/2024\/05\/image003-4-300x288.png 300w, https:\/\/assets.www.gsbet888.com\/uploads\/2024\/05\/image003-4-680x653.png 680w, https:\/\/assets.www.gsbet888.com\/uploads\/2024\/05\/image003-4-768x738.png 768w\" sizes=\"(max-width: 820px) 100vw, 820px\" \/><\/p>\n<p style=\"text-align: justify;\">Fig.1a-e\u8be6\u7ec6\u4ecb\u7ecd\u4e86\u8be5\u96c6\u6210\u5f0f\u53ef\u7a7f\u6234\u8bbe\u5907\u7684\u5404\u90e8\u4ef6\u7ec4\u6210\u548c\u4f20\u611f\u539f\u7406\u3002\u8be5\u53ef\u7a7f\u6234\u7cfb\u7edf\u7531\u4e00\u6b21\u6027\u5fae\u9488\u8d34\u7247\u548c\u53ef\u91cd\u590d\u4f7f\u7528\u7684\u7535\u5b50\u8bbe\u5907\uff08\u65e0\u7ebf\u67d4\u6027\u7535\u8def\u677f\u3001\u529f\u80fd\u6027\u70ed\u5851\u6027\u805a\u6c28\u916f\u8584\u819c\uff09\u7ec4\u6210\u3002\u5176\u4e2d\uff0c\u4e00\u6b21\u6027\u5fae\u9488\uff08Microneedle, MN\u00a0\uff09\u8d34\u7247\u7531\u4e09\u7535\u6781\u9635\u5217\u4ee5\u7d27\u51d1\u7684\u65b9\u5f0f\u5236\u4e09\u5408\u4e00\u8d34\u7247\u3002\u91c7\u7528\u55b7\u5370\u548c\u94f8\u9020\u6280\u672f\u5236\u5907\u4e86\u7531\u7b2c\u4e00\u5c42\u94f6\u548c\u7b2c\u4e8c\u5c42\u78b3\u7eb3\u7c73\u7ba1\u7ec4\u6210\u7684\u529f\u80fd\u6027\u70ed\u5851\u6027\u805a\u6c28\u916f\uff08Thermoplastic polyurethane, TPU\uff09\u3002\u5c06\u5177\u6709\u9633\u6781\u548c\u9634\u6781\u56fe\u6848\u7684\u529f\u80fd\u5316TPU\u819c\u4e0e\u65e0\u7ebf\u67d4\u6027\u7535\u8def\u677f(flexible circuit board, FCB)\u5355\u72ec\u8fde\u63a5\uff0c\u4f7f\u5176\u80fd\u591f\u901a\u8fc7\u53cd\u5411\u79bb\u5b50\u7535\u6cf3\u5bcc\u96c6\u6838\u9178\u3002\u96c6\u6210\u53ef\u7a7f\u6234\u7cfb\u7edf\u7684\u7b2c\u4e09\u90e8\u5206\u662f\u81ea\u884c\u8bbe\u8ba1\u548c\u5b9a\u5236\u7684FCB\uff0c\u7528\u4e8e\u7535\u6e90\u548c\u4fe1\u53f7\u7ba1\u7406\u3002<\/p>\n<p style=\"text-align: justify;\">TDN-17\u88ab\u7528\u4f5c\u56fa\u5b9a\u5728\u77f3\u58a8\u70ef\u8868\u9762\u7684\u6700\u4f73\u7eb3\u7c73\u652f\u67b6\uff0c\u5728TDN-17\u4e0a\uff0cNgAgo\u86cb\u767d\u4f5c\u4e3a\u4e00\u79cd\u539f\u6838\u86cb\u767d\u88ab\u56fa\u5b9a\u5f62\u6210NgAgo\/\u5f15\u5bfcDNA\u590d\u5408\u7269\uff0c\u8bb0\u4f5cNgAgo- gDNA\u3002\u5728gDNA (24nt 5 &#8216; -\u78f7\u9178\u5316\u5355\u94feDNA)\u7684\u6307\u5bfc\u4e0b\uff0c\u901a\u8fc7\u78b1\u57fa\u914d\u5bf9\u641c\u7d22\u6574\u4e2a\u76ee\u6807\u5e8f\u5217\u3002\u4e00\u65e6\u5339\u914d\uff0c\u56fa\u5b9a\u5316\u7684NgAgo-gDNA\u5c31\u53ef\u4ee5\u4e0e\u76ee\u6807\u6838\u9178\u7ed3\u5408\uff0c\u7531\u4e8e\u5019\u9009gDNA\u7684\u5b58\u5728\uff0c\u8fd9\u79cd\u7ed3\u5408\u5177\u6709\u660e\u786e\u7684\u7a33\u5b9a\u6027\u3002<\/p>\n<p style=\"text-align: justify;\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-49774 size-full\" src=\"https:\/\/assets.www.gsbet888.com\/uploads\/2024\/05\/image005-4.png\" alt=\"\" width=\"1021\" height=\"731\" srcset=\"https:\/\/assets.www.gsbet888.com\/uploads\/2024\/05\/image005-4.png 1021w, https:\/\/assets.www.gsbet888.com\/uploads\/2024\/05\/image005-4-300x215.png 300w, https:\/\/assets.www.gsbet888.com\/uploads\/2024\/05\/image005-4-680x487.png 680w, https:\/\/assets.www.gsbet888.com\/uploads\/2024\/05\/image005-4-768x550.png 768w\" sizes=\"(max-width: 1021px) 100vw, 1021px\" \/><\/p>\n<p style=\"text-align: justify;\">\u4e3a\u4f18\u5316TDN\u4fee\u9970\u7684\u751f\u7269\u4f20\u611f\u754c\u9762\uff0c\u4f5c\u8005\u5236\u5907\u4e86\u4fee\u9970\u6709\u4e0d\u540c\u9ad8\u5ea6TDNs\u7684\u751f\u7269\u4f20\u611f\u754c\u9762\uff0c\u5e76\u5728TDNs\u9876\u7aef\u951a\u5b9aNgAgo-gDNA\u590d\u5408\u7269\u3002\u5982\u56fe2c\uff0c\u4e0e\u6ca1\u6709TDN\u7684\u7cfb\u7edf\u76f8\u6bd4\uff0c\u5177\u6709TDN\u7684\u751f\u7269\u4f20\u611f\u7cfb\u7edf\u5728~0.28V\u7684\u7535\u4f4d\u4e0b\u7535\u6d41\u4fe1\u53f7\u589e\u5f3a\uff0c\u4e14\u4fe1\u53f7\u5f3a\u5ea6\u968f\u9776\u6807\u6d53\u5ea6\u7684\u589e\u52a0\u800c\u63d0\u9ad8\u3002\u7ed3\u679c\u8868\u660e\uff0c\u4f7f\u7528\u9ad8\u5ea6\u4e3a5.8 nm\u7684TDN-17\u65f6\u4ea7\u751f\u7684\u7535\u6d41\u4fe1\u53f7\u6700\u5f3a\uff0c\u4e14\u68c0\u6d4b\u9650\u4e0e\u4e0d\u542bTDN\u7684\u5bf9\u7167\u7ec4\u76f8\u6bd4\u964d\u4f4e\u4e86\u4e24\u4e2a\u6570\u91cf\u7ea7\uff0c\u8fbe\u52303\u00d710<sup>-12<\/sup>\u00a0M\u3002\u5f53TDN\u9ad8\u5ea6\u7ee7\u7eed\u589e\u52a0\u65f6\uff0c\u8f93\u51fa\u4fe1\u53f7\u7684\u5f3a\u5ea6\u548c\u68c0\u6d4b\u7075\u654f\u5ea6\u5747\u964d\u4f4e\u3002\u8fd9\u8bc1\u660e\u5728\u5408\u9002\u7684\u9ad8\u5ea6\u8303\u56f4\u5185\uff0c\u7a7a\u95f4\u9ad8\u5ea6\u5bf9\u754c\u9762\u7684Donnan\u7535\u4f4d\u6709\u4e00\u5b9a\u7684\u8c03\u8282\u4f5c\u7528\u3002\u56fe\u00a02d-f\u00a0\u4f7f\u7528\u5546\u7528\u5fae\u7535\u6781\u5bf9\u82af\u7247\u4e0aTDN-17\/NgAgo-gDNA\u00a0\u7684\u68c0\u6d4b\u9650\u8fdb\u884c\u7814\u7a76\uff0810 V\u53cd\u5411\u79bb\u5b50\u7535\u6e17\uff09\uff0c\u7ed3\u679c\u8868\u660e\u82af\u7247\u4e0aTDN-17\/NgAgo-gDNA\u68c0\u6d4b\u9650\u53ef\u8fbe3\u00d710<sup>-16<\/sup>\u00a0M\u3002<\/p>\n<p style=\"text-align: justify;\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-49775 size-full\" src=\"https:\/\/assets.www.gsbet888.com\/uploads\/2024\/05\/image007-3.png\" alt=\"\" width=\"965\" height=\"847\" srcset=\"https:\/\/assets.www.gsbet888.com\/uploads\/2024\/05\/image007-3.png 965w, https:\/\/assets.www.gsbet888.com\/uploads\/2024\/05\/image007-3-300x263.png 300w, https:\/\/assets.www.gsbet888.com\/uploads\/2024\/05\/image007-3-680x597.png 680w, https:\/\/assets.www.gsbet888.com\/uploads\/2024\/05\/image007-3-768x674.png 768w\" sizes=\"(max-width: 965px) 100vw, 965px\" \/><\/p>\n<p style=\"text-align: justify;\">\u5de5\u7a0bDNA\u5f15\u5bfc\u7684NgAgo\u7cfb\u7edf\u80fd\u5426\u8bc6\u522b\u548c\u7ed3\u5408\u9776\u6838\u9178\uff0c\u8fd9\u5bf9\u540e\u7eed\u7684\u7814\u7a76\u5177\u6709\u91cd\u8981\u610f\u4e49\u3002\u57fapAgo\u6280\u672f\u7684\u539f\u7406\uff0c\u63a8\u6d4b\u590d\u5408\u7269\u53ef\u80fd\u5728gDNA\u7684\u5f15\u5bfc\u4e0b\u641c\u7d22\u6574\u4e2a\u6838\u9178\u5e8f\u5217\uff0c\u5176\u4e2d\u4e00\u4e2a5 &#8216; -\u78f7\u9178\u5316\u768424-nt\u7279\u5f02\u6027\u5e8f\u5217\u4e0e\u76ee\u6807\u5339\u914d\u3002\u4e00\u65e6\u5339\u914d\uff0c\u590d\u5408\u4f53\u5c31\u53ef\u4ee5\u76f4\u63a5\u4e0e\u76ee\u6807\u7279\u5f02\u6027\u7ed3\u5408\u3002\u4f5c\u8005\u901a\u8fc7\u8868\u9762\u7b49\u79bb\u5b50\u4f53\u5171\u632f\uff08SPR\uff09\u6d4b\u8bd5\u4e86NgAgo-gDNA\u4e0e\u9776\u6807\u6838\u9178\u7ed3\u5408\u4eb2\u548c\u529b(Fig.3a-c)\u3002\u7ed3\u679c\u663e\u793a\uff0cEBV cfDNA\u9776\u6807\u6d53\u5ea6\u57283.125 nM-100 nM\u5185\u65f6\uff0cNgAgo-gDNA\u00a0\u590d\u5408\u7269\u7684\u76f8\u5bf9\u54cd\u5e94\u5f3a\u5ea6\u4e0e\u9776\u6807\u6d53\u5ea6\u6210\u7ebf\u6027\u5173\u7cfb\u3002NgAgo-gDNA\u00a0\u590d\u5408\u7269\u5bf9EBV cfDNA\u7684KD\u4e3a5.49\u00d710<sup>-9<\/sup>\u00a0M<sup>-1<\/sup>\uff0c\u8be5\u7cfb\u7edf\u5177\u6709\u9ad8\u9776\u5411\u4eb2\u548c\u529b\uff0c\u4e14\u7565\u5f3a\u4e8e\u00a0CRISPR-dCas9\uff08KD\u4e3a1\u00d710<sup>-9<\/sup>\u00a0M<sup>-1<\/sup>\uff09<sup>[3]<\/sup>\u3002\u4e3a\u4e86\u6d88\u9664\u77f3\u58a8\u70ef\u8868\u9762\u7684\u5e72\u6270\uff0c\u4f5c\u8005\u6784\u5efa\u4e86\u4e00\u4e2a\u7b80\u5355\u7684\u7535\u5316\u5b66\u751f\u7269\u4f20\u611f\u5668\u6765\u8fdb\u4e00\u6b65\u7814\u7a76\u5176\u7ed3\u5408\u4eb2\u548c\u529b\u3002\u5728Fig.3d\u4e2d\uff0c\u4e0eNTC\u7ec4\u76f8\u6bd4\uff0cgDNA 3\u7ec4\u6709\u660e\u663e\u7684\u4fe1\u53f7\u54cd\u5e94\uff0c\u4e14\u5dee\u5f02\u663e\u8457\u3002\u6b64\u5916\uff0c\u65e0\u8bba\u77f3\u58a8\u70ef\u754c\u9762\u5982\u4f55\uff0cgDNA 3\u7ec4\u90fd\u5177\u6709\u6700\u5927\u7535\u6d41\u3002Fig.3e\u4e3aNgAgo-gDNA\u7684\u9884\u6d4b\u6a21\u578b\u3002NgAgo\u86cb\u767d\u5177\u6709\u5178\u578b\u7684N\u672b\u7aef\u3001PAZ\u3001MID\u3001PIWI\u7ed3\u6784\u57df\u4ee5\u53caRepA\u7ed3\u6784\u57df\u3002gDNA\u4e0e\u7531N\u672b\u7aef\u3001PAZ\u3001MID\u548cPIWI\u5f62\u6210\u7684\u53e3\u888b\u7a33\u5b9a\u7ed3\u5408\u3002NgAgo-gDNA\u590d\u5408\u7269\u8868\u9762\u5927\u90e8\u5206\u5e26\u6709\u8d1f\u7535\u8377\uff0c\u800cRepA\u7ed3\u6784\u57df\u7684\u8868\u9762\u5219\u5e26\u6709\u6b63\u7535\u8377\uff0c\u4f7f\u5176\u9002\u5408\u4e8e\u4e0e\u5e26\u8d1f\u7535\u8377\u7684\u5e95\u7269\uff08\u5982DNA\u6216RNA\uff09\u76f8\u4e92\u4f5c\u7528\u3002\u4ee5\u4e0a\u7ed3\u679c\u8bc1\u660eNg\u7cfb\u7edf\u80fd\u591f\u6709\u6548\u5730\u8bc6\u522b\u5e76\u7ed3\u5408EBV cfDNA\u9776\u6807\u3002<\/p>\n<p style=\"text-align: justify;\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-49776 size-full\" src=\"https:\/\/assets.www.gsbet888.com\/uploads\/2024\/05\/image009-3.png\" alt=\"\" width=\"787\" height=\"861\" srcset=\"https:\/\/assets.www.gsbet888.com\/uploads\/2024\/05\/image009-3.png 787w, https:\/\/assets.www.gsbet888.com\/uploads\/2024\/05\/image009-3-274x300.png 274w, https:\/\/assets.www.gsbet888.com\/uploads\/2024\/05\/image009-3-680x744.png 680w, https:\/\/assets.www.gsbet888.com\/uploads\/2024\/05\/image009-3-768x840.png 768w\" sizes=\"(max-width: 787px) 100vw, 787px\" \/><\/p>\n<p style=\"text-align: justify;\">\u968f\u540e\uff0c\u4f5c\u8005\u9a8c\u8bc1\u4e86TDN-Ng MNs\u5728\u4f53\u5916\u5b9e\u9a8c\u4e2d\u7684\u7075\u654f\u5ea6\u3001\u5b9a\u91cf\u68c0\u6d4b\u3001\u957f\u671f\u7a33\u5b9a\u6027\u3001\u7279\u5f02\u6027\u548c\u6297\u5e72\u6270\u6027\u80fd(Fig.4a-f)\u3002TDN-Ng MNs\u53ef\u6301\u7eed\u8bc6\u522b\u5e76\u7ed3\u5408\u9776\u6807\u00a0cfDNA\uff0c\u68c0\u6d4b\u9650\u4e3a\u00a03\u00d710-16\u00a0M\u3002\u542b\u9776\u6807cfDNA\u7684\u5b9e\u9a8c\u7ec4\u548cNTC\u4e4b\u95f4\u5b58\u5728\u663e\u8457\u5dee\u5f02\uff0c\u4e14\u7535\u6d41\u54cd\u5e94\u5bf9\u65f6\u95f4\u7684\u5173\u7cfb\u66f2\u7ebf\u7684\u659c\u7387\u4e0e\u9776\u6807cfDNA\u00a0\u6d53\u5ea6\u6210\u6b63\u6bd4\u3002\u6b64\u5916\uff0c\u7ed3\u679c\u8868\u660e\u53cd\u5e94\u572830 min\u5185\u9010\u6e10\u8d8b\u4e8e\u7a33\u5b9a\uff0c\u6bd4\u8be5\u56e2\u961f\u4e4b\u524d\u62a5\u9053\u7684CRISPR MN\u8d34\u7247\u7684\u76d1\u6d4b\u65f6\u95f4\uff0875 min\uff09\u66f4\u77ed\uff0c\u8868\u660e\u8be5\u7814\u7a76\u6240\u6784\u5efa\u7684\u57fa\u4e8eTDN\u7684NgAgo-gDNA\u7cfb\u7edf\u7684\u53cd\u5e94\u52a8\u529b\u5b66\u66f4\u5feb\u3002\u4e3a\u9a8c\u8bc1TDN-Ng MNs\u68c0\u6d4b\u9776\u6807\u6838\u9178\u7684\u957f\u671f\u7a33\u5b9a\u6027\uff0c\u4f5c\u8005\u5c06TDN-Ng MN\u8d34\u7247\u4fdd\u5b58\u5728\u95f4\u8d28\u6db2\uff08ISF\uff0c37\u00b0C\uff0cpH 7.4\uff09\u4e2d\uff0c\u5e76\u572810V\u53cd\u5411\u79bb\u5b50\u7535\u6e17\u6761\u4ef6\u4e0b\u76d1\u6d4b3\u00d710\u221214M\u7684\u9776\u6807\u6838\u9178\u3002\u5728\u524d16\u5929\u5185\uff0cSt\u503c\u4fdd\u6301\u7a33\u5b9a\uff0cC.V.\u4e3a4.98%\u3002\u867d\u7136\u4ece\u7b2c17\u5929\u5f00\u59cbSt\u503c\u4e0b\u964d\uff0c\u4f46\u4ecd\u53ef\u89c2\u5bdf\u5230\u660e\u663e\u7684\u201cS\u201d\u5f62\u4fe1\u53f7\u66f2\u7ebf\u3002\u4ee5\u4e0a\u7ed3\u679c\u8868\u660e\uff0cTDN-Ng MN\u8d34\u7247\u7528\u4e8e\u4f53\u5916\u6838\u9178\u76d1\u6d4b\u65f6\u5728\u5408\u7406\u7684C.V.\u8303\u56f4\u5185\u8868\u73b0\u51fa16\u5929\u7684\u957f\u671f\u7a33\u5b9a\u6027\u3002\u6b64\u5916\uff0c\u7ed3\u679c\u8fd8\u8bc1\u660e TDN-Ng MN\u8d34\u7247\u53ef\u4ee5\u8010\u53d70.3 nM\u7684\u975e\u7279\u5f02\u6027\u6838\u9178\u548c80%\u7684\u80ce\u725b\u8840\u6e05\uff08FBS\uff09\u5e72\u6270\uff0c\u7279\u5f02\u6027\u548c\u6297\u5e72\u6270\u6027\u80fd\u597d\u3002\u4e0e\u4eba\u4f53ISF\u7684\u603b\u86cb\u767d\u6d53\u5ea6\uff0820.6 mg\/mL\uff09\u76f8\u6bd4\uff0c\u8fd9\u79cd\u5bf980%FBS\uff0823 mg\/mL\uff09\u7684\u4f18\u5f02\u6297\u5e72\u6270\u80fd\u529b\u53ef\u80fd\u4f7fTDN Ng-MN\u8d34\u7247\u5e94\u7528\u4e8e\u5b9e\u9645\u3002<\/p>\n<p style=\"text-align: justify;\">\u6839\u636e\u5148\u524d\u7684\u7814\u7a76\uff0cNgAgo-gDNA\u590d\u5408\u7269\u88ab\u62a5\u9053\u5177\u6709\u6572\u4f4e\u57fa\u56e0\u548c\u8bf1\u5bfc\u8f6c\u5f55\u6c89\u9ed8\u7684\u80fd\u529b\u3002\u56e0\u6b64\uff0c\u4f5c\u8005\u6000\u7591RNA\u4e5f\u53ef\u80fd\u88ab\u4ed6\u4eec\u7684TDN-Ng\u7cfb\u7edf\u8bc6\u522b\u3002\u4f5c\u8005\u9996\u5148\u7b5b\u9009RNA-168\u548cRNA-351\u5e8f\u5217\u4f5c\u4e3a\u75be\u75c5\u76f8\u5173\u751f\u7269\u6807\u5fd7\u7269\uff0c\u7136\u540e\u4f7f\u7528\u5de5\u7a0b\u5316\u7684NgAgo-gDNA\u5728ISF\u4e2d\u9274\u5b9a\u8fd9\u4e24\u79cdRNA\u3002\u5728Fig.4g,h\u4e2d\uff0c\u4e0e\u5bf9\u7167\u7ec4\u76f8\u6bd4\uff0cRNA-168\u548cRNA-351\u4e0eNgAgo-gDNA\u590d\u5408\u7269\u7ed3\u5408\u5e76\u8bf1\u5bfc\u4e86\u663e\u8457\u7684\u7535\u6d41\u53cd\u5e94\u3002\u5728\u672c\u7814\u7a76\u4e2d\uff0c\u4f5c\u8005\u9009\u62e9RNA-168\u548cRNA-351\u4f5c\u4e3a\u9776\u6807\uff0c\u9a8c\u8bc1TDN-Ng MN\u8d34\u7247\u7684\u5b9e\u65f6\u76d1\u6d4b\u80fd\u529b\u3002\u57283\u00d710<sup>\u2212<\/sup><sup>14<\/sup>\u00a0M\u9776\u6807\u7684\u7b2c\u4e00\u6b65\uff0c\u968f\u7740RNA-168\u6216RNA-351\u6d53\u5ea6\u7684\u589e\u52a0\uff0c\u4fe1\u53f7\u589e\u52a0\u3002\u4fe1\u53f7\u5728\u7b2c2\u6b65\uff083\u00d710<sup>\u2212<\/sup><sup>13<\/sup>\u00a0M\uff09\u548c\u7b2c3\u6b65\uff083\u00d710<sup>\u2212<\/sup><sup>12<\/sup>\u00a0M\uff09\u8fde\u7eed\u589e\u52a0\uff0c\u8868\u660e\u8be5\u7cfb\u7edf\u80fd\u591f\u5b9e\u65f6\u76d1\u6d4b\u9776RNA\u7684\u52a8\u6001\u53d8\u5316\u3002TDN-Ng MN\u5e73\u53f0\u8fd8\u53ef\u4ee5\u5b9e\u65f6\u76d1\u6d4b\u5176\u4ed6\u5e94\u7528\uff0c\u5982\u91d1\u9ec4\u8272\u8461\u8404\u7403\u83cc\u548c\u94dc\u7eff\u5047\u5355\u80de\u83cc\u3002<\/p>\n<p style=\"text-align: justify;\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-49777 size-full\" src=\"https:\/\/assets.www.gsbet888.com\/uploads\/2024\/05\/image011-1.png\" alt=\"\" width=\"711\" height=\"893\" srcset=\"https:\/\/assets.www.gsbet888.com\/uploads\/2024\/05\/image011-1.png 711w, https:\/\/assets.www.gsbet888.com\/uploads\/2024\/05\/image011-1-239x300.png 239w, https:\/\/assets.www.gsbet888.com\/uploads\/2024\/05\/image011-1-680x854.png 680w\" sizes=\"(max-width: 711px) 100vw, 711px\" \/><\/p>\n<p style=\"text-align: justify;\">\u6700\u540e\uff0c\u4f5c\u8005\u8fdb\u4e00\u6b65\u9a8c\u8bc1\u4e86TDN-Ng MNs\u5bf9\u4f53\u5185\u6838\u9178\u7684\u5b9e\u65f6\u76d1\u6d4b\u6027\u80fd\u3002\u636e\u62a5\u9053\uff0cEBV\u00a0\u76f8\u5173\u6838\u9178\u751f\u7269\u6807\u5fd7\u7269\u4e0e\u8113\u6bd2\u75c7\u6709\u5173\uff0c\u4e14CNE\u7ec6\u80de\u7cfb\u4e2d\u5b58\u5728cfDNA\u6216RNA\u3002\u56e0\u6b64\uff0c\u4f5c\u8005\u9996\u5148\u7528\u8367\u5149\u7d20\u9176\u62a5\u544a\u57fa\u56e0\uff08Luc\uff09\u5bf9CNE\u7ec6\u80de\u7cfb\u8fdb\u884c\u91cd\u7f16\u7a0b\uff0c\u7136\u540e\u5c06\u5176\u76ae\u4e0b\u63a5\u79cd\u52308\u5468\u5927\u7684\u96cc\u6027BALB\/c\u88f8\u9f20\u4f53\u5185\u4ee5\u6784\u5efaEBV-\u5c0f\u9f20\u6a21\u578b\u3002\u63a5\u7740\uff0c\u4f5c\u8005\u5c06TDN-Ng MN\u8d34\u7247\u7528\u4e8e\u4e0a\u8ff0\u5c0f\u9f20\u6a21\u578b\u3002\u7ed3\u679c\u5982Fig.5a-h\u663e\u793a\uff0cTDN-Ng MN\u8d34\u7247\u4e0e\u91d1\u6807\u51c6RT-PCR\u76d1\u6d4b\u7ed3\u679c\u4e00\u81f4\uff0cTDN-Ng MN\u8d34\u7247\u53ef\u7528\u4e8eEBV\u6a21\u578b\u5c0f\u9f20\u4f53\u5185RNA\u9776\u6807\u7684\u5b9e\u65f6\u76d1\u6d4b\uff0c\u4e14\u7528\u4e8e\u4f53\u5185\u6838\u9178\u5b9e\u65f6\u76d1\u6d4b\u7684TDN-Ng MN\u8d34\u7247\u7684\u572816\u5929\u5185\u5177\u6709\u53ef\u9760\u7a33\u5b9a\u6027\uff0c\u53ef\u6ee1\u8db3ICU\u60a3\u8005\u75be\u75c5\u76d1\u6d4b\u7684\u4e34\u5e8a\u8981\u6c42\u3002<\/p>\n<h4 style=\"text-align: justify;\">\u603b\u7ed3\uff1a<\/h4>\n<p style=\"text-align: justify;\">\u7efc\u4e0a\u6240\u8ff0\uff0c\u672c\u7814\u7a76\u63d0\u51fa\u4e86\u65e0\u7ebf\u96c6\u6210\u53ef\u7a7f\u6234\u7535\u5b50\u8bbe\u5907\uff0c\u901a\u8fc7\u5de5\u7a0b\u751f\u7269\u4f20\u611f\u63a5\u53e3\u5b9e\u65f6\u76d1\u6d4b\u8d25\u8840\u75c7\u76f8\u5173\u52a8\u7269\u6a21\u578b\u7684\u7eb5\u5411\u6838\u9178\u3002\u57fa\u4e8eTDN\u548cNg\u7cfb\u7edf\u7684\u534f\u540c\u4f5c\u7528\uff0c\u53ef\u7a7f\u6234\u7cfb\u7edf\u80fd\u591f\u8fde\u7eed\u8ddf\u8e2a\u4f53\u5185\u65e0\u7ec6\u80deDNA\u548cRNA\u9776\u70b9\u7684\u52a8\u6001\u53d8\u5316\uff0c\u7075\u654f\u5ea6\u4e3a0.3 fM\uff0c\u5728\u4f53\u518516\u5929\u5185\u5177\u6709\u53ef\u9760\u7684\u7a33\u5b9a\u6027\u3002\u4f5c\u8005\u8fd8\u8ba8\u8bba\u4e86TDN-17\u968f\u7a7a\u95f4\u9ad8\u5ea6\u589e\u52a0\u5bf9TDN-Ng\u7cfb\u7edf\u7684\u7075\u654f\u5ea6\u589e\u5f3a\u548c\u5b9e\u65f6\u76d1\u6d4b\u52a8\u6001\u5e73\u8861\u7684\u673a\u7406\u3002\u76ee\u524d\u8fd9\u9879\u5de5\u4f5c\u7684\u65e0\u6269\u589e\u7b56\u7565\u4ee3\u8868\u4e86\u57fa\u4e8e\u6838\u9178\u7684\u53ef\u7a7f\u6234\u8bbe\u5907\u7684\u98de\u8dc3\uff0c\u5e76\u52a0\u901f\u4e86\u7528\u4e8eICU\u533b\u7597\u4fdd\u5065\u7ba1\u7406\u7684\u4e0b\u4e00\u4ee3\u751f\u7269\u4f20\u611f\u5668\u7684\u51fa\u73b0\u3002\u8fd9\u9879\u5de5\u4f5c\u7684\u540e\u7eed\u53d1\u5c55\u5c06\u96c6\u4e2d\u5728\u76f4\u63a5\u8bb0\u5f55\u6ce2\u52a8\u7684\u8f93\u5165\u4fe1\u53f7(\u589e\u52a0\uff0c\u51cf\u5c11\u548c\u6062\u590d)\uff0c\u66f4\u9ad8\u4eb2\u548c\u529b\u7684\u6784\u5efa\uff0c\u751f\u7269\u4f20\u611f\u819c\u518d\u751f\u548c\u81ea\u52a8\u6570\u636e\u91c7\u96c6\u3002\u540c\u65f6\uff0c\u57fa\u4e8e\u4eba\u5de5\u667a\u80fd\u7684\u673a\u5668\u5b66\u4e60\u53ef\u7528\u4e8e\u534f\u52a9\u9884\u6d4bICU\u533b\u7597\u62a5\u8b66\u7ba1\u7406\u7684\u6570\u636e\u91c7\u96c6\u3002<\/p>\n<h4 style=\"text-align: justify;\">\u53c2\u8003\u6587\u732e\uff1a<\/h4>\n<ul style=\"text-align: justify;\">\n<li>[1] P. Fozouni, S. Son, M. D\u00edaz de Le\u00f3n Derby, G. J. Knott, C. N. Gray, M. V. D\u2019Ambrosio, C. Zhao, N. A. Switz, G. R. Kumar, S. I. Stephens, D. Boehm, C.-L. Tsou, J. Shu, A. Bhuiya, M. Armstrong, A. R. Harris, P.-Y. Chen, J. M. Osterloh, A. Meyer-Franke, B. Joehnk, K. Walcott, A. Sil, C. Langelier, K. S. Pollard, E. D. Crawford, A. S. Puschnik, M. Phelps, A. Kistler, J. L. DeRisi, J. A. Doudna, D. A. Fletcher, M. Ott, <em>Cell<\/em> <strong>2021<\/strong>, 184, 323-333.e329. DOI: <a href=\"https:\/\/doi.org\/10.1016\/j.cell.2020.12.001\">10.1016\/j.cell.2020.12.001<\/a><\/li>\n<li>[2] D. S. Yang, R. Ghaffari, J. A. Rogers, Science 2023, 379, 760-761. <a href=\"https:\/\/www.science.org\/doi\/10.1126\/science.abq5916\">DOI: 10.1126\/science.abq5916<\/a><\/li>\n<li>[3] J. Yang, Y. Song, X. Deng, J. A. Vanegas, Z. You, Y. Zhang, Z. Weng, L. Avery, K. D. Dieckhaus, A. Peddi, Y. Gao, Y. Zhang, X. Gao, <em>Nat. Chem. Biol. <\/em><strong>2023<\/strong>, 19, 45-54. DOI:<a href=\"https:\/\/www.nature.com\/articles\/s41589-022-01135-y#citeas\">10.1038\/s41589-022-01135-y<\/a><\/li>\n<\/ul>\n<p style=\"text-align: justify;\">\u672c\u6587\u7248\u6743\u5c5e\u4e8e\u00a0Chem-Station\u5316\u5b66\u7a7a\u95f4\uff0c \u6b22\u8fce\u70b9\u51fb\u6309\u94ae\u5206\u4eab\uff0c\u672a\u7ecf\u8bb8\u53ef\uff0c\u8c22\u7edd\u8f6c\u8f7d.<\/p>\n","protected":false},"excerpt":{"rendered":"\u4f5c\u8005\uff1a\u82cf\u6253\u6c34 \u5bfc\u8bfb\uff1a \u8fd1\u65e5\uff0c\u590d\u65e6\u5927\u5b66\u65b9\u96ea\u6069\u6559\u6388\u56e2\u961f\u5728Nat. Commun.\u4e2d\u53d1\u8868\u8bba\u6587\uff0c\u62a5\u9053\u4e86\u4e00\u79cd\u57fa\u4e8e\u56db\u9762\u4f53\u7eb3\u7c73\u7ed3\u6784\u7684\u55dc\u76d0\u83ccArgonaute\uff08NgAgo\uff09\u6784\u5efa\u7684\u53ef\u7a7f\u6234\u5fae\u9488\u4f20\u611f\u5668\uff0c\u7528\u4e8e\u957f\u671f\u7a33\u5b9a\u76d1\u6d4b\u4f53\u5185\u8d25\u8840\u75c7\u7684\u8d85\u75d5\u91cf\u672a\u6269\u589e\u6838\u9178\u3002 Long-Term Monitoring of Ultratrace Nucleic Acids Using Tetrahedral Nanostructure-Based Ngago on Wearable Microneedles. Yang, B.; Wang, H.; Kong, J.; Fang, X.\u00a0Nat. Commun. 2024, 15 (1), 1936. DOI: 10.1038\/s41467-024-46215-w \u6b63\u6587\uff1a \u8fd1\u5e74\u6765\uff0c\u57fa\u56e0\u68c0\u6d4b\u6280\u672f\u7684\u53d1\u5c55\u4e3a\u6838\u9178\u8bca\u65ad\u8bc6\u522b\u75be\u75c5\u63d0\u4f9b\u4e86\u5f3a\u5927\u5de5\u5177\u3002\u5176\u4e2dPCR\u3001\u7b49\u6e29\u6838\u9178\u6269\u589e\u548cCRISPR\u6280\u672f\u7ed3\u5408\u7684\u5168\u57fa\u56e0\u7ec4\u6d4b\u5e8f\u53ef\u63d0\u4f9b\u591a\u79cd\u75c5\u7406\u751f\u7406\u4fe1\u606f\uff0c\u4f46\u5927\u591a\u6280\u672f\u5747\u9762\u4e34\u5468\u8f6c\u65f6\u95f4\u957f\u3001\u8bbe\u5907\u7b28\u91cd\u7b49\u95ee\u9898[1]\u3002\u65b0\u5174\u7684\u53ef\u7a7f\u6234\u6280\u672f\u5728\u4e2a\u6027\u5316\u533b\u7597\u3001\u533b\u7597\u7269\u8054\u7f51\u548c\u5b9e\u65f6\u75be\u75c5\u8bca\u65ad\u4e2d\u63d0\u4f9b\u4e86\u53d8\u9769\u6027\u6280\u672f\uff0c\u53ef\u80fd\u89e3\u51b3\u4f20\u7edf\u6838\u9178\u5206\u5b50\u8bca\u65ad\u5c40\u9650\u3002\u8fd9\u7c7b\u5148\u8fdb\u7684\u53ef\u7a7f\u6234\u8bbe\u5907\u53ef\u4ee5\u76d1\u6d4b\u6df1\u5c42\u7ec4\u7ec7\u3001\u5fc3\u810f\u529f\u80fd\u3001\u6162\u6027\u611f\u67d3\u6027\u4f24\u53e3\u3001\u4f53\u6e29\u8c03\u8282\u548c\u80bf\u7624\u6d88\u9000\uff0c\u4ee5\u53ca\u8840\u7cd6\u3001\u6c34\u5206\u6d41\u5931\u548c\u6c57\u6db2\u7535\u89e3\u8d28\u76d1\u6d4b[2]\u3002\u53ef\u7a7f\u6234\u8bbe\u5907\u53ef\u80fd\u4e3a\u6301\u7eed\u76d1\u6d4b\u63d0\u4f9b\u65b0\u9014\u5f84\uff0c\u4f46\u5728ICU\u573a\u666f\u4e2d\u4ecd\u5b58\u5728\u5c40\u9650\uff0c\u7279\u522b\u662f\u5728\u76d1\u6d4b\u4f4e\u4e30\u5ea6\u75be\u75c5\u76f8\u5173\u6e38\u79bbDNA\u6216RNA\u751f\u7269\u6807\u5fd7\u7269\u65b9\u9762\u3002 \u57fa\u4e8e\u6b64\uff0c\u590d\u65e6\u5927\u5b66\u65b9\u96ea\u6069\u6559\u6388\u56e2\u961f\u62a5\u544a\u4e86\u57fa\u4e8e\u56db\u9762\u4f53 DNA \u7eb3\u7c73\u7ed3\u6784 \uff08TDN\uff09 \u548c\u539f\u6838 argonaute \u6280\u672f\u7684\u5b8c\u5168\u96c6\u6210\u7684\u53ef\u7a7f\u6234\u7535\u5b50\u8bbe\u5907\uff0c\u5b9e\u65f6\u76d1\u6d4b\u7531 EBV\u3001\u91d1\u9ec4\u8272\u8461\u8404\u7403\u83cc\u548c\u94dc\u7eff\u5047\u5355\u80de\u83cc (staphylococcus aureus, SA) and pseudomonas aeruginosa, PA)\u5f15\u8d77\u7684\u8113\u6bd2\u75c7\u76f8\u5173\u7684cfDNA\u548cRNA\uff0c\u4ece\u800c\u6269\u5927\u4e86\u5728ICU\u60c5\u51b5\u4e0b\u53d1\u75c5\u65f6\u76d1\u6d4b\u8113\u6bd2\u75c7\u7684\u53ef\u80fd\u6027\u3002 Fig.1a-e\u8be6\u7ec6\u4ecb\u7ecd\u4e86\u8be5\u96c6\u6210\u5f0f\u53ef\u7a7f\u6234\u8bbe\u5907\u7684\u5404\u90e8\u4ef6\u7ec4\u6210\u548c\u4f20\u611f\u539f\u7406\u3002\u8be5\u53ef\u7a7f\u6234\u7cfb\u7edf\u7531\u4e00\u6b21\u6027\u5fae\u9488\u8d34\u7247\u548c\u53ef\u91cd\u590d\u4f7f\u7528\u7684\u7535\u5b50\u8bbe\u5907\uff08\u65e0\u7ebf\u67d4\u6027\u7535\u8def\u677f\u3001\u529f\u80fd\u6027\u70ed\u5851\u6027\u805a\u6c28\u916f\u8584\u819c\uff09\u7ec4\u6210\u3002\u5176\u4e2d\uff0c\u4e00\u6b21\u6027\u5fae\u9488\uff08Microneedle, MN\u00a0\uff09\u8d34\u7247\u7531\u4e09\u7535\u6781\u9635\u5217\u4ee5\u7d27\u51d1\u7684\u65b9\u5f0f\u5236\u4e09\u5408\u4e00\u8d34\u7247\u3002\u91c7\u7528\u55b7\u5370\u548c\u94f8\u9020\u6280\u672f\u5236\u5907\u4e86\u7531\u7b2c\u4e00\u5c42\u94f6\u548c\u7b2c\u4e8c\u5c42\u78b3\u7eb3\u7c73\u7ba1\u7ec4\u6210\u7684\u529f\u80fd\u6027\u70ed\u5851\u6027\u805a\u6c28\u916f\uff08Thermoplastic polyurethane, TPU\uff09\u3002\u5c06\u5177\u6709\u9633\u6781\u548c\u9634\u6781\u56fe\u6848\u7684\u529f\u80fd\u5316TPU\u819c\u4e0e\u65e0\u7ebf\u67d4\u6027\u7535\u8def\u677f(flexible circuit board, FCB)\u5355\u72ec\u8fde\u63a5\uff0c\u4f7f\u5176\u80fd\u591f\u901a\u8fc7\u53cd\u5411\u79bb\u5b50\u7535\u6cf3\u5bcc\u96c6\u6838\u9178\u3002\u96c6\u6210\u53ef\u7a7f\u6234\u7cfb\u7edf\u7684\u7b2c\u4e09\u90e8\u5206\u662f\u81ea\u884c\u8bbe\u8ba1\u548c\u5b9a\u5236\u7684FCB\uff0c\u7528\u4e8e\u7535\u6e90\u548c\u4fe1\u53f7\u7ba1\u7406\u3002 TDN-17\u88ab\u7528\u4f5c\u56fa\u5b9a\u5728\u77f3\u58a8\u70ef\u8868\u9762\u7684\u6700\u4f73\u7eb3\u7c73\u652f\u67b6\uff0c\u5728TDN-17\u4e0a\uff0cNgAgo\u86cb\u767d\u4f5c\u4e3a\u4e00\u79cd\u539f\u6838\u86cb\u767d\u88ab\u56fa\u5b9a\u5f62\u6210NgAgo\/\u5f15\u5bfcDNA\u590d\u5408\u7269\uff0c\u8bb0\u4f5cNgAgo- gDNA\u3002\u5728gDNA (24nt 5 &#8216; -\u78f7\u9178\u5316\u5355\u94feDNA)\u7684\u6307\u5bfc\u4e0b\uff0c\u901a\u8fc7\u78b1\u57fa\u914d\u5bf9\u641c\u7d22\u6574\u4e2a\u76ee\u6807\u5e8f\u5217\u3002\u4e00\u65e6\u5339\u914d\uff0c\u56fa\u5b9a\u5316\u7684NgAgo-gDNA\u5c31\u53ef\u4ee5\u4e0e\u76ee\u6807\u6838\u9178\u7ed3\u5408\uff0c\u7531\u4e8e\u5019\u9009gDNA\u7684\u5b58\u5728\uff0c\u8fd9\u79cd\u7ed3\u5408\u5177\u6709\u660e\u786e\u7684\u7a33\u5b9a\u6027\u3002 \u4e3a\u4f18\u5316TDN\u4fee\u9970\u7684\u751f\u7269\u4f20\u611f\u754c\u9762\uff0c\u4f5c\u8005\u5236\u5907\u4e86\u4fee\u9970\u6709\u4e0d\u540c\u9ad8\u5ea6TDNs\u7684\u751f\u7269\u4f20\u611f\u754c\u9762\uff0c\u5e76\u5728TDNs\u9876\u7aef\u951a\u5b9aNgAgo-gDNA\u590d\u5408\u7269\u3002\u5982\u56fe2c\uff0c\u4e0e\u6ca1\u6709TDN\u7684\u7cfb\u7edf\u76f8\u6bd4\uff0c\u5177\u6709TDN\u7684\u751f\u7269\u4f20\u611f\u7cfb\u7edf\u5728~0.28V\u7684\u7535\u4f4d\u4e0b\u7535\u6d41\u4fe1\u53f7\u589e\u5f3a\uff0c\u4e14\u4fe1\u53f7\u5f3a\u5ea6\u968f\u9776\u6807\u6d53\u5ea6\u7684\u589e\u52a0\u800c\u63d0\u9ad8\u3002\u7ed3\u679c\u8868\u660e\uff0c\u4f7f\u7528\u9ad8\u5ea6\u4e3a5.8 nm\u7684TDN-17\u65f6\u4ea7\u751f\u7684\u7535\u6d41\u4fe1\u53f7\u6700\u5f3a\uff0c\u4e14\u68c0\u6d4b\u9650\u4e0e\u4e0d\u542bTDN\u7684\u5bf9\u7167\u7ec4\u76f8\u6bd4\u964d\u4f4e\u4e86\u4e24\u4e2a\u6570\u91cf\u7ea7\uff0c\u8fbe\u52303\u00d710-12\u00a0M\u3002\u5f53TDN\u9ad8\u5ea6\u7ee7\u7eed\u589e\u52a0\u65f6\uff0c\u8f93\u51fa\u4fe1\u53f7\u7684\u5f3a\u5ea6\u548c\u68c0\u6d4b\u7075\u654f\u5ea6\u5747\u964d\u4f4e\u3002\u8fd9\u8bc1\u660e\u5728\u5408\u9002\u7684\u9ad8\u5ea6\u8303\u56f4\u5185\uff0c\u7a7a\u95f4\u9ad8\u5ea6\u5bf9\u754c\u9762\u7684Donnan\u7535\u4f4d\u6709\u4e00\u5b9a\u7684\u8c03\u8282\u4f5c\u7528\u3002\u56fe\u00a02d-f\u00a0\u4f7f\u7528\u5546\u7528\u5fae\u7535\u6781\u5bf9\u82af\u7247\u4e0aTDN-17\/NgAgo-gDNA\u00a0\u7684\u68c0\u6d4b\u9650\u8fdb\u884c\u7814\u7a76\uff0810 V\u53cd\u5411\u79bb\u5b50\u7535\u6e17\uff09\uff0c\u7ed3\u679c\u8868\u660e\u82af\u7247\u4e0aTDN-17\/NgAgo-gDNA\u68c0\u6d4b\u9650\u53ef\u8fbe3\u00d710-16\u00a0M\u3002 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\u53c2\u8003\u6587\u732e\uff1a [1] P. 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