{"id":51769,"date":"2024-11-28T09:00:57","date_gmt":"2024-11-28T01:00:57","guid":{"rendered":"\/\/www.gsbet888.com\/?p=51769"},"modified":"2024-11-28T09:01:26","modified_gmt":"2024-11-28T01:01:26","slug":"%e8%a5%bf%e6%b9%96%e5%a4%a7%e5%ad%a6%e5%8f%b6%e5%ae%87%e8%bd%a9%e8%af%be%e9%a2%98%e7%bb%84nat-chem-%ef%bc%9a-%e6%96%b0%e5%9e%8b%e5%8e%bb%e9%a5%b1%e5%92%8c%e5%8c%96%e9%85%b6%ef%bc%9a%e8%a7%a3","status":"publish","type":"post","link":"\/\/www.gsbet888.com\/%e5%8c%96%e5%ad%a6%e6%9d%82%e8%ae%b0\/recentpaper\/2024\/11\/%e8%a5%bf%e6%b9%96%e5%a4%a7%e5%ad%a6%e5%8f%b6%e5%ae%87%e8%bd%a9%e8%af%be%e9%a2%98%e7%bb%84nat-chem-%ef%bc%9a-%e6%96%b0%e5%9e%8b%e5%8e%bb%e9%a5%b1%e5%92%8c%e5%8c%96%e9%85%b6%ef%bc%9a%e8%a7%a3.html","title":{"rendered":"\u897f\u6e56\u5927\u5b66\u53f6\u5b87\u8f69\u8bfe\u9898\u7ec4Nat. Chem.\uff1a \u65b0\u578b\u53bb\u9971\u548c\u5316\u9176\uff1a\u89e3\u9501\u70ef\u8fd8\u539f\u9176\u7684\u975e\u5929\u7136\u53cd\u5e94\u6027\u5b9e\u73b0\u9009\u62e9\u6027\u8131\u6c22"},"content":{"rendered":"

\u4f5c\u8005\uff1a\u738b\u8f89<\/strong><\/p>\n

\u5bfc\u8bfb\uff1a<\/strong><\/h4>\n

\u8fd1\u65e5\uff0c\u897f\u6e56\u5927\u5b66\u53f6\u5b87\u8f69\u8bfe\u9898\u7ec4\u5728Nature Chemistry<\/em>\u4e0a\u53d1\u8868\u4e86\u9898\u4e3a\u300cUnmasking the Reverse Catalytic Activity of \u2018Ene\u2019-Reductases for Asymmetric Carbonyl Desaturation<\/strong>\u300d\u7684\u7814\u7a76\u8bba\u6587\u3002\u8be5\u5c0f\u7ec4\u4ece\u4e00\u7c7b\u5e95\u7269\u8303\u56f4\u597d\u3001\u70ed\u7a33\u5b9a\u6027\u9ad8\u3001\u6613\u4e8e\u8868\u8fbe\u7684\u70ef\u8fd8\u539f\u9176\u51fa\u53d1\uff0c\u6210\u529f\u628a\u5176\u4ece\u8fd8\u539f\u9176\u6539\u9020\u6210\u53bb\u9971\u548c\u5316\u9176\uff0c\u5efa\u7acb\u4e86\u4e00\u4e2a\u901a\u7528\u7684\u9176\u50ac\u5316\u7fb0\u57fa\u53bb\u9971\u548c\u5316\u53cd\u5e94\u5e73\u53f0\uff0c\u5408\u6210\u4e86\u4e00\u7cfb\u5217\u542b\u6709\u8fdc\u7aef\u56db\u7ea7\u624b\u6027\u4e2d\u5fc3\u7684\u9ad8\u4ef7\u503c\u73af\u5df1\u70ef\u916e\u4ea7\u7269\u3002\u6b64\u9176\u50ac\u5316\u53cd\u5e94\u4f53\u7cfb\u6761\u4ef6\u6e29\u548c\u3001\u64cd\u4f5c\u7b80\u5355\u3001\u6613\u4e8e\u653e\u5927\uff0c\u5c55\u73b0\u51fa\u4e0e\u5df2\u6709\u7684\u5316\u5b66\u5408\u6210\u65b9\u6cd5\u4e92\u8865\u7684\u53cd\u5e94\u6027\u548c\u9009\u62e9\u6027<\/p>\n

\u201cUnmasking the Reverse Catalytic Activity of \u2018Ene\u2019-Reductases for Asymmetric Carbonyl Desaturation<\/p>\n

Hui Wang, Bin Gao, Heli Cheng, Shixuan Cao, Xinyi Ma, Yinjuan Chen, Yuxuan Ye*<\/p>\n

Nat.Chem. 2024, ASAP. DOI: https:\/\/doi.org\/10.1038\/s41557-024-01671-1<\/a>\u201d<\/p><\/blockquote>\n

\"\"<\/p>\n

\u6b63\u6587<\/strong>\uff1a<\/h4>\n

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\u56fe4. \u5176\u5b83\u7c7b\u522b\u73af\u5df1\u916e\u7684\u53bb\u5bf9\u79f0\u5316\u8131\u6c22\u53cd\u5e94\u3002\u56fe\u7247\u6765\u6e90\uff1aNat. Chem.<\/em><\/p>\n

\u6b64\u9176\u50ac\u5316\u53cd\u5e94\u4f53\u7cfb\u6761\u4ef6\u6e29\u548c\uff0c\u64cd\u4f5c\u7b80\u5355\uff0c\u53ef\u4ee5\u5f88\u5bb9\u6613\u8fdb\u884c\u653e\u5927\u3002\u4f5c\u8005\u9996\u5148\u5bf9\u6a21\u578b\u53cd\u5e94\u8fdb\u884c\u4e86\u514b\u7ea7\u89c4\u6a21\u653e\u5927\uff1a\u4f7f\u7528\u7ec6\u80de\u88c2\u89e3\u6db2\u53ef\u4ee588% \u7684\u4ea7\u7387\uff0c>99:1 er\u83b7\u5f97\u4ea7\u72692<\/strong>\uff0c\u4f7f\u7528\u51bb\u5e72\u88c2\u89e3\u6db2\u53ef\u4ee581% \u7684\u4ea7\u7387\u3001>99:1 er\u5b9e\u73b0\u53cd\u5e94 (\u56fe5a)\u3002\u6b64\u5916\uff0c\u4f5c\u8005\u4ee5\u514b\u7ea7\u89c4\u6a21\u9ad8\u6548\u5236\u5907\u4e86 (+)-Mesembrine\uff0c\u4e00\u79cd\u5177\u6709\u8840\u6e05\u7d20\u518d\u6444\u53d6\u6291\u5236\u6d3b\u6027\u7684\u751f\u7269\u78b1\u3002\u901a\u8fc7\u9176\u4fc3\u53bb\u9971\u548c\u5316\u8fc7\u7a0b\u5236\u5907\u7684\u73af\u5df1\u70ef\u916e\u4ea7\u72692<\/strong>\u662f\u5e94\u7528\u5e7f\u6cdb\u7684\u5408\u6210\u524d\u4f53\uff0c\u80fd\u591f\u9ad8\u7acb\u4f53\u7279\u5f02\u6027\u7684\u8f6c\u5316\u4e3a\u6709\u4ef7\u503c\u7684\u624b\u6027\u5316\u5408\u726962<\/strong>\uff0c\u6216\u662f\u7ecf\u5386\u5e95\u7269\u63a7\u5236\u7684\u975e\u5bf9\u6620\u9009\u62e9\u6027Diel-Alder\u53cd\u5e94\uff0c\u751f\u6210\u4e86\u5e26\u6709\u4e09\u4e2a\u76f8\u90bb\u7acb\u4f53\u4e2d\u5fc3\u7684\u516d\u6c22\u8418\u916e63 <\/strong>(\u56fe5c)\u3002\"\"<\/p>\n

\u56fe5. \u514b\u7ea7\u89c4\u6a21\u653e\u5927\u548c\u884d\u751f\u5316\u3002\u56fe\u7247\u6765\u6e90\uff1aNat. Chem.<\/em><\/p>\n

\u6700\u540e\uff0c\u4f5c\u8005\u5bf9\u8fd9\u4e00\u53cd\u5e94\u7684\u673a\u7406\u8fdb\u884c\u4e86\u63a2\u7d22\u3002\u6b64\u8131\u6c22\u53cd\u5e94\u7684\u53ef\u80fd\u673a\u7406\u662f (\u56fe6a): \u5e95\u7269\u5728\u9176\u7684\u6d3b\u6027\u7a7a\u8154\u7ed3\u5408\u540e\uff0c\u4fdd\u5b88\u7684\u916a\u6c28\u9178\u6b8b\u57fa\u9634\u79bb\u5b50 (GkOYE3\u7684Y169) \u5bf9\u5e95\u7269\u8fdb\u884c\u03b1-\u53bb\u8d28\u5b50\u5316\uff0c\u5f62\u6210\u70ef\u9187\u4e2d\u95f4\u4f53Int1<\/strong>\u3002\u968f\u540e\uff0c\u4e00\u79cd\u53cd\u5e94\u673a\u7406\u662f\u7ecf\u5386\u8def\u5f84A<\/strong>\uff0cInt1<\/strong>\u5c06\u03b2-\u6c22\u8d1f\u8f6c\u79fb\u81f3FMNox<\/sub><\/strong>\uff0c\u5f97\u5230\u53bb\u9971\u548c\u5316\u7684\u70ef\u916e\u4ea7\u72692<\/strong>\u548cFMNhq<\/sub><\/strong>\u3002\u6b64\u53cd\u5e94\u9014\u5f84\u4e0eERED\u50ac\u5316\u7684\u5929\u7136\u8fd8\u539f\u8fc7\u7a0b\u76f8\u53cd\uff0c\u7b26\u5408\u5fae\u89c2\u53ef\u9006\u6027\u539f\u7406\u3002\u53e6\u5916\u4e00\u79cd\u53cd\u5e94\u673a\u7406\u662f\u7ecf\u5386\u8def\u5f84B<\/strong>\uff0cInt1<\/strong>\u4e0eFMNox<\/sub><\/strong>\u8fdb\u884c\u5355\u7535\u5b50\u8f6c\u79fb (SET)\uff0c\u751f\u6210\u76f8\u5e94\u7684\u03b1-\u9170\u57fa\u4e2d\u95f4\u4f53Int2<\/strong>\u548c\u9ec4\u7d20\u534a\u918cFMNsq<\/sub><\/strong>\uff0c\u7136\u540e\u8fdb\u884c\u8d28\u5b50\u8f6c\u79fb (PT) \u548c\u53e6\u4e00\u4e2a\u5355\u7535\u5b50\u8f6c\u79fb (SET) \u62102<\/strong>\u548cFMNhq<\/sub><\/strong>\u3002\u6b64\u53cd\u5e94\u9014\u5f84\u4e0e\u8fd1\u671f\u62a5\u9053\u7684ERED\u50ac\u5316\u7684\u5355\u7535\u5b50\u6c27\u5316\u53cd\u5e94\u7c7b\u4f3c\u3002\u6700\u540e\uff0c\u5728\u9176\u50ac\u5316\u5242\u91ca\u653e\u4ea7\u72692<\/strong>\uff0cFMNhq<\/sub><\/strong>\u4e0e\u7a7a\u6c14\u4e2d\u7684\u5206\u5b50\u6c27\u53d1\u751f\u53cd\u5e94\uff0c\u7ecf\u5386\u9ec4\u7d20\u6c22\u8fc7\u6c27\u5316\u7269\u4e2d\u95f4\u4f53\uff0c\u5f62\u6210\u8fc7\u6c27\u5316\u6c22\u5e76\u91cd\u65b0\u751f\u6210\u6c27\u5316\u6001\u8f85\u56e0\u5b50FMNox<\/sub><\/strong>\u3002<\/p>\n

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\u56fe6.\u53cd\u5e94\u673a\u7406\uff1b\u6c22\/\u6c18\u4ea4\u6362\u5b9e\u9a8c\uff1b\u52a8\u529b\u5b66\u5b9e\u9a8c\u3002\u56fe\u7247\u6765\u6e90\uff1aNat. Chem.<\/em><\/p>\n

\u603b\u7ed3<\/strong><\/h4>\n

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\u901a\u8baf\u4f5c\u8005\u7b80\u4ecb<\/strong><\/h4>\n

\"\"<\/p>\n

\u53f6\u5b87\u8f69\u535a\u58eb2013\u5e74\u4e8e\u5317\u4eac\u5927\u5b66\u5316\u5b66\u4e0e\u5206\u5b50\u5de5\u7a0b\u5b66\u9662\u83b7\u5f97\u5b66\u58eb\u5b66\u4f4d\uff0c\u6307\u5bfc\u6559\u5e08\u4e3a\u738b\u5251\u6ce2\u6559\u6388\u548c\u5f20\u8273\u6559\u6388\u30022018\u5e74\u5728\u7f8e\u56fd\u9ebb\u7701\u7406\u5de5\u5b66\u9662\u83b7\u5f97\u535a\u58eb\u5b66\u4f4d\uff0c\u6307\u5bfc\u6559\u5e08\u4e3aStephen Buchwald\u6559\u6388\u30022019\u20132022\u5e74\u5728\u7f8e\u56fd\u666e\u6797\u65af\u987f\u5927\u5b66\u548c\u5eb7\u5948\u5c14\u5927\u5b66\u5f00\u5c55\u535a\u58eb\u540e\u7814\u7a76\uff0c\u5408\u4f5c\u6559\u5e08\u4e3aTodd Hyster\u6559\u6388\u3002\u53f6\u5b87\u8f69\u535a\u58eb\u4e8e2022\u5e749\u6708\u5168\u804c\u52a0\u5165\u897f\u6e56\u5927\u5b66\u7406\u5b66\u9662\u62c5\u4efb\u7279\u8058\u7814\u7a76\u5458\uff0c\u4e3b\u8981\u7814\u7a76\u65b9\u5411\u4e3a\u65b0\u578b\u9176\u50ac\u5316\u975e\u5929\u7136\u53cd\u5e94\u7684\u53d1\u5c55\u548c\u5e94\u7528\u3002\u53f6\u5b87\u8f69\u8bfe\u9898\u7ec4\u73b0\u9762\u5411\u56fd\u5185\u5916\u62db\u8058\u535a\u58eb\u7814\u7a76\u751f\u3001\u535a\u58eb\u540e\u3001\u79d1\u7814\u52a9\u7406\uff0c\u6b22\u8fce\u5bf9\u9176\u50ac\u5316\u611f\u5174\u8da3\u7684\u540c\u5b66\u7533\u8bf7\u3002\u8bfe\u9898\u7ec4\u4e3b\u9875\uff1a<\/strong>ye.lab.westlake.edu.cn<\/strong><\/a>\u3002<\/strong><\/p>\n

\n

\u672c\u6587\u7248\u6743\u5c5e\u4e8e\u00a0Chem-Station\u5316\u5b66\u7a7a\u95f4\uff0c \u6b22\u8fce\u70b9\u51fb\u6309\u94ae\u5206\u4eab\uff0c\u672a\u7ecf\u8bb8\u53ef\uff0c\u8c22\u7edd\u8f6c\u8f7d.<\/p>\n

\u5173\u6ce8Chem-Station\u6296\u97f3\u53f7\uff1a79473891841<\/p>\n

\u8bf7\u767b\u9646TCI\u8bd5\u5242\u5b98\u7f51\u67e5\u770b\u66f4\u591a\u5185\u5bb9<\/h5>\n
https:\/\/www.tcichemicals.com\/CN\/zh\/<\/h5>\n<\/div>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"\u4f5c\u8005\uff1a\u738b\u8f89 \u5bfc\u8bfb\uff1a \u8fd1\u65e5\uff0c\u897f\u6e56\u5927\u5b66\u53f6\u5b87\u8f69\u8bfe\u9898\u7ec4\u5728Nature Chemistry\u4e0a\u53d1\u8868\u4e86\u9898\u4e3a\u300cUnmasking the Reverse Catalytic Activity of \u2018Ene\u2019-Reductases for Asymmetric Carbonyl Desaturation\u300d\u7684\u7814\u7a76\u8bba\u6587\u3002\u8be5\u5c0f\u7ec4\u4ece\u4e00\u7c7b\u5e95\u7269\u8303\u56f4\u597d\u3001\u70ed\u7a33\u5b9a\u6027\u9ad8\u3001\u6613\u4e8e\u8868\u8fbe\u7684\u70ef\u8fd8\u539f\u9176\u51fa\u53d1\uff0c\u6210\u529f\u628a\u5176\u4ece\u8fd8\u539f\u9176\u6539\u9020\u6210\u53bb\u9971\u548c\u5316\u9176\uff0c\u5efa\u7acb\u4e86\u4e00\u4e2a\u901a\u7528\u7684\u9176\u50ac\u5316\u7fb0\u57fa\u53bb\u9971\u548c\u5316\u53cd\u5e94\u5e73\u53f0\uff0c\u5408\u6210\u4e86\u4e00\u7cfb\u5217\u542b\u6709\u8fdc\u7aef\u56db\u7ea7\u624b\u6027\u4e2d\u5fc3\u7684\u9ad8\u4ef7\u503c\u73af\u5df1\u70ef\u916e\u4ea7\u7269\u3002\u6b64\u9176\u50ac\u5316\u53cd\u5e94\u4f53\u7cfb\u6761\u4ef6\u6e29\u548c\u3001\u64cd\u4f5c\u7b80\u5355\u3001\u6613\u4e8e\u653e\u5927\uff0c\u5c55\u73b0\u51fa\u4e0e\u5df2\u6709\u7684\u5316\u5b66\u5408\u6210\u65b9\u6cd5\u4e92\u8865\u7684\u53cd\u5e94\u6027\u548c\u9009\u62e9\u6027 \u201cUnmasking the Reverse Catalytic Activity of \u2018Ene\u2019-Reductases for Asymmetric Carbonyl Desaturation Hui Wang, Bin Gao, Heli Cheng, Shixuan Cao, Xinyi Ma, Yinjuan Chen, Yuxuan Ye* Nat.Chem. 2024, ASAP. DOI: https:\/\/doi.org\/10.1038\/s41557-024-01671-1\u201d \u6b63\u6587\uff1a \u03b1,\u03b2-\u4e0d\u9971\u548c\u7fb0\u57fa\u5316\u5408\u7269\u5728\u5236\u836f\u3001\u519c\u836f\u548c\u6750\u6599\u7b49\u591a\u4e2a\u9886\u57df\u5177\u6709\u91cd\u8981\u7684\u4f5c\u7528\u3002\u5728\u9884\u5148\u6784\u5efa\u7684\u5206\u5b50\u6846\u67b6\u4e2d\u76f4\u63a5\u8131\u6c22\u5f15\u5165\u4e0e\u7fb0\u57fa\u76f8\u90bb\u7684C=C\u53cc\u952e\u662f\u6784\u5efa\u03b1,\u03b2-\u4e0d\u9971\u548c\u7fb0\u57fa\u5316\u5408\u7269\u7684\u7406\u60f3\u7b56\u7565\u4e4b\u4e00\u3002\u8fd1\u51e0\u5341\u5e74\u6765\uff0c\u5408\u6210\u5316\u5b66\u5bb6\u53d1\u5c55\u4e86\u8bb8\u591a\u50ac\u5316\u4f53\u7cfb\u6765\u5b9e\u73b0\u6b64\u8fc7\u7a0b\u3002\u4f8b\u5982\u7fb0\u57fa\u03b1\u4f4d\u5364\u5316-\u6d88\u9664\u7684\u4e32\u8054\u8fc7\u7a0b\uff1bSaegusa-Ito\u6c27\u5316\uff1b\u9ad8\u4ef7\u7898\u6c27\u5316\uff1b\u65b0\u578b\u6c27\u5316\u5242 (SeO2\u3001N-\u53d4\u4e01\u57fa\u82ef\u57fa\u4e9a\u78fa\u9170\u4e9a\u80fa\u6c2f\u3001IBX\u548cAZADO\u7b49) \u4ecb\u5bfc\u7684\u76f4\u63a5\u8131\u6c22\uff1b\u8fc7\u6e21\u91d1\u5c5e\u50ac\u5316\uff1b\u7535\u50ac\u5316\u7b49 (\u56fe1a)\u3002\u7136\u800c\uff0c\u76ee\u524d\u5df2\u6709\u7684\u65b9\u6cd5\u8981\u4e48\u9700\u8981\u591a\u6b65\u5408\u6210\uff0c\u8981\u4e48\u9700\u8981\u5728\u8f83\u5f3a\u6c27\u5316\u6027\u6761\u4ef6\u4e0b\u8fdb\u884c\uff0c\u6216\u4f7f\u7528\u8f83\u9ad8\u5f53\u91cf\u7684\u91d1\u5c5e\u50ac\u5316\u5242\u3002\u66f4\u91cd\u8981\u7684\u662f\uff0c\u76ee\u524d\u8fd8\u6ca1\u6709\u4e00\u4e2a\u666e\u9002\u7684\u50ac\u5316\u4f53\u7cfb\uff0c\u53ef\u4ee5\u5728\u53bb\u9971\u548c\u5316\u8fc7\u7a0b\u4e2d\u5b9e\u73b0\u7acb\u4f53\u5316\u5b66\u7684\u7cbe\u786e\u63a7\u5236\u3002\u8fd9\u4e9b\u5c40\u9650\u90fd\u9650\u5236\u4e86\u76f4\u63a5\u8131\u6c22\u53bb\u9971\u548c\u5316\u53cd\u5e94\u5728\u5408\u6210\u5316\u5b66\u4e2d\u66f4\u5e7f\u6cdb\u7684\u5e94\u7528\u3002 \u8fd1\u5e74\u6765\uff0c\u9176\u50ac\u5316\u5242\u56e0\u5176\u9009\u62e9\u6027\u63a7\u5236\u7cbe\u786e\u3001\u6613\u4e8e\u6539\u9020\u8fdb\u5316\u548c\u7eff\u8272\u73af\u4fdd\u7b49\u72ec\u7279\u4f18\u52bf\uff0c\u8d8a\u6765\u8d8a\u53d7\u5230\u5408\u6210\u5316\u5b66\u5bb6\u7684\u5173\u6ce8\u3002\u81ea\u7136\u754c\u4e2d\u5b58\u5728\u4e00\u4e9b\u5929\u7136\u50ac\u5316\u7fb0\u57fa\u53bb\u9971\u548c\u5316\u53cd\u5e94\u7684\u9176\uff0c\u4f46\u5b83\u4eec\u7684\u5e95\u7269\u666e\u9002\u6027\u5dee\uff0c\u96be\u4ee5\u4f5c\u4e3a\u50ac\u5316\u5242\u88ab\u5e7f\u6cdb\u5e94\u7528\u3002\u4e3a\u4e86\u514b\u670d\u8fd9\u4e9b\u5c40\u9650\uff0c\u897f\u6e56\u5927\u5b66\u53f6\u5b87\u8f69\u8bfe\u9898\u7ec4\u4ece\u4e00\u7c7b\u5e95\u7269\u8303\u56f4\u597d\u3001\u70ed\u7a33\u5b9a\u6027\u9ad8\u3001\u6613\u4e8e\u8868\u8fbe\u7684\u70ef\u8fd8\u539f\u9176\u51fa\u53d1\uff0c\u6210\u529f\u628a\u5176\u4ece\u8fd8\u539f\u9176\u6539\u9020\u6210\u53bb\u9971\u548c\u5316\u9176 (\u56fe1b)\uff0c\u5efa\u7acb\u4e86\u4e00\u4e2a\u901a\u7528\u7684\u9176\u50ac\u5316\u7fb0\u57fa\u53bb\u9971\u548c\u5316\u53cd\u5e94\u5e73\u53f0\uff0c\u5408\u6210\u4e86\u4e00\u7cfb\u5217\u542b\u6709\u8fdc\u7aef\u56db\u7ea7\u624b\u6027\u4e2d\u5fc3\u7684\u9ad8\u4ef7\u503c\u73af\u5df1\u70ef\u916e\u4ea7\u7269 (\u56fe1c)\u3002\u6b64\u9176\u50ac\u5316\u53cd\u5e94\u4f53\u7cfb\u6761\u4ef6\u6e29\u548c\u3001\u64cd\u4f5c\u7b80\u5355\u3001\u6613\u4e8e\u653e\u5927\uff0c\u5c55\u73b0\u51fa\u4e0e\u5df2\u6709\u7684\u5316\u5b66\u5408\u6210\u65b9\u6cd5\u4e92\u8865\u7684\u53cd\u5e94\u6027\u548c\u9009\u62e9\u6027\u3002\u76f8\u5173\u6210\u679c\u53d1\u8868\u4e8eNature Chemistry\u4e0a\u3002 \u56fe1. \u9176\u50ac\u5316\u7fb0\u57fa\u53bb\u9971\u548c\u5316\u53cd\u5e94\u3002\u56fe\u7247\u6765\u6e90\uff1aNat. Chem. \u4f5c\u8005\u9996\u5148\u5efa\u7acb\u4e86\u7ed3\u6784\u591a\u6837\u7684\u70ef\u8fd8\u539f\u9176\u5e93\uff0c\u4ee54-\u7532\u57fa-4-\u82ef\u57fa-\u73af\u5df1\u916e1\u4f5c\u4e3a\u6a21\u578b\u5e95\u7269\uff0c\u5728\u6c27\u6c14\u5b58\u5728\u7684\u6761\u4ef6\u4e0b\u5bf9ERED\u7684\u8131\u6c22\u53cd\u5e94\u6027\u8fdb\u884c\u8bc4\u4f30\uff0c\u53d1\u73b0CrS\u548cGkOYE\u8868\u73b0\u51fa\u8f83\u597d\u7684\u6d3b\u6027\u548c\u5bf9\u6620\u9009\u62e9\u6027\u63a7\u5236\u3002\u5f53\u6e29\u5ea6\u5347\u9ad8\u65f6\uff0c\u53bb\u9971\u548c\u5316\u53cd\u5e94\u6027\u63d0\u9ad8 (\u56fe2a)\u3002\u968f\u540e\u4f5c\u8005\u4ee5\u91ce\u751f\u578bCrS\u548cGkOYE\u4e3a\u8d77\u70b9\uff0c\u5229\u7528\u5b9a\u5411\u8fdb\u5316\u6280\u672f\u63d0\u9ad8\u9176\u50ac\u5316\u5242\u7684\u53cd\u5e94\u6548\u7387\u3002\u5355\u7a81\u53d8\u4f53CrS1 (Y27L)\u3001CrS2 (Y27C) \u548cCrS3 (Y27T) \u5206\u522b\u4ee579% (98:2 er), 88% (98:2 er)\u548c81% (97:2 er) \u5f97\u5230\u4ea7\u72692\uff1bGkOYE-WT\u7ecf\u8fc7\u4e09\u8f6e\u8fdb\u5316\u5f97\u5230\u4e00\u4e2a\u4e09\u91cd\u7a81\u53d8\u4f53GkOYE3 (D73G\u3001C26S\u3001Y28V)\uff0c\u53ef\u4ee5\u4ee5\u5f88\u9ad8\u7684\u6548\u7387\u50ac\u53162\u7684\u751f\u6210 (96%\uff0c>99:1 er) (\u56fe2b)\u3002 \u56fe2. \u53cd\u5e94\u4f18\u5316\u548c\u5b9a\u5411\u8fdb\u5316\u3002\u56fe\u7247\u6765\u6e90\uff1aNat. Chem. \u63a5\u7740\uff0c\u4f5c\u8005\u6536\u96c6\u4e86\u5728\u5b9a\u5411\u8fdb\u5316\u4e2d\u83b7\u5f97\u7684\u6709\u76ca\u7a81\u53d8\u4f53\uff0c\u5efa\u7acb\u4e86\u53bb\u9971\u548c\u5316\u9176\u5e93\uff0c\u5e76\u5bf9\u53cd\u5e94\u7684\u5e95\u7269\u8303\u56f4\u8fdb\u884c\u4e86\u63a2\u7d22\u3002\u4f5c\u8005\u9996\u5148\u6d4b\u8bd5\u4e86\u591a\u79cd4-\u70f7\u57fa-4-\u82b3\u57fa\u53d6\u4ee3\u7684\u73af\u5df1\u916e\u5e95\u7269\uff0c\u5747\u5177\u6709\u826f\u597d\u7684\u8010\u53d7\u6027 (\u56fe3)\u3002\u5f53\u4f7f\u7528\u542b2-\u6c28\u57fa\u82ef\u57fa\u7684\u73af\u5df1\u916e\u4f5c\u4e3a\u8d77\u59cb\u539f\u65998\u65f6\uff0c\u4ee599% (>99:1 er, >20:1 dr) \u7684\u6536\u7387\u5f97\u5230\u73af\u5316\u7684\u4ea7\u7269\u4e8c\u6c22\u5432\u54da\u916e9\u3002\u503c\u5f97\u6ce8\u610f\u7684\u662f\uff0c\u8fd9\u79cd\u751f\u7269\u50ac\u5316\u53bb\u9971\u548c\u5316\u65b9\u6cd5\u5bf9\u80fa\u548c\u786b\u919a\u7b49\u6c27\u5316\u654f\u611f\u5b98\u80fd\u56e2\u8868\u73b0\u51fa\u9ad8\u7684\u517c\u5bb9\u6027\u3002\u6b64\u5916\uff0c\u7f3a\u7535\u5b50\u548c\u5bcc\u7535\u5b50\u6742\u73af\u90fd\u662f\u80fd\u591f\u5728\u8be5\u53cd\u5e94\u6761\u4ef6\u4e0b\u88ab\u517c\u5bb9\u3002\u9664\u7532\u57fa\u5916\u7684\u5404\u79cd\u70f7\u57fa\u53d6\u4ee3\u57fa\u5e95\u7269\u4e5f\u53ef\u4ee5\u5b9e\u73b0\u9ad8\u7acb\u4f53\u9009\u62e9\u6027\u7684\u8131\u6c22\u3002\u4ece\u76f8\u5e94\u76843-\u7f9f\u4e19\u57fa\u5e95\u726918\u51fa\u53d1\u65f6\uff0c\u53ef\u4ee599%\u6536\u7387 (>99:1 er\uff0c>20:1 dr) \u5f97\u5230\u73af\u5316\u4ea7\u7269\u516b\u6c22\u8272\u70ef-7-\u916e19\u3002\u6b64\u5916\uff0c\u87ba\u73af\u5e95\u7269 (20–23) \u4e5f\u80fd\u5f88\u597d\u5730\u8f6c\u5316\u3002\u7136\u800c\uff0c4-\u7f9f\u57fa\u82ef\u57fa\u7684\u73af\u5df1\u916e24\u4e0d\u80fd\u88ab\u9176\u50ac\u5316\u4f53\u7cfb\u517c\u5bb9\uff0c\u53ef\u80fd\u662f\u7531\u4e8e\u8be5\u5e95\u7269\u662f\u9176\u7684\u6291\u5236\u5242\u3002 \u56fe3. 4-\u70f7\u57fa-4-\u82b3\u57fa\u73af\u5df1\u916e\u53bb\u5bf9\u79f0\u5316\u8131\u6c22\u53cd\u5e94\u3002\u56fe\u7247\u6765\u6e90\uff1aNat. 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Chem. \u6b64\u9176\u50ac\u5316\u53cd\u5e94\u4f53\u7cfb\u6761\u4ef6\u6e29\u548c\uff0c\u64cd\u4f5c\u7b80\u5355\uff0c\u53ef\u4ee5\u5f88\u5bb9\u6613\u8fdb\u884c\u653e\u5927\u3002\u4f5c\u8005\u9996\u5148\u5bf9\u6a21\u578b\u53cd\u5e94\u8fdb\u884c\u4e86\u514b\u7ea7\u89c4\u6a21\u653e\u5927\uff1a\u4f7f\u7528\u7ec6\u80de\u88c2\u89e3\u6db2\u53ef\u4ee588% \u7684\u4ea7\u7387\uff0c>99:1 er\u83b7\u5f97\u4ea7\u72692\uff0c\u4f7f\u7528\u51bb\u5e72\u88c2\u89e3\u6db2\u53ef\u4ee581% \u7684\u4ea7\u7387\u3001>99:1 er\u5b9e\u73b0\u53cd\u5e94 (\u56fe5a)\u3002\u6b64\u5916\uff0c\u4f5c\u8005\u4ee5\u514b\u7ea7\u89c4\u6a21\u9ad8\u6548\u5236\u5907\u4e86 (+)-Mesembrine\uff0c\u4e00\u79cd\u5177\u6709\u8840\u6e05\u7d20\u518d\u6444\u53d6\u6291\u5236\u6d3b\u6027\u7684\u751f\u7269\u78b1\u3002\u901a\u8fc7\u9176\u4fc3\u53bb\u9971\u548c\u5316\u8fc7\u7a0b\u5236\u5907\u7684\u73af\u5df1\u70ef\u916e\u4ea7\u72692\u662f\u5e94\u7528\u5e7f\u6cdb\u7684\u5408\u6210\u524d\u4f53\uff0c\u80fd\u591f\u9ad8\u7acb\u4f53\u7279\u5f02\u6027\u7684\u8f6c\u5316\u4e3a\u6709\u4ef7\u503c\u7684\u624b\u6027\u5316\u5408\u726962\uff0c\u6216\u662f\u7ecf\u5386\u5e95\u7269\u63a7\u5236\u7684\u975e\u5bf9\u6620\u9009\u62e9\u6027Diel-Alder\u53cd\u5e94\uff0c\u751f\u6210\u4e86\u5e26\u6709\u4e09\u4e2a\u76f8\u90bb\u7acb\u4f53\u4e2d\u5fc3\u7684\u516d\u6c22\u8418\u916e63 (\u56fe5c)\u3002 \u56fe5. \u514b\u7ea7\u89c4\u6a21\u653e\u5927\u548c\u884d\u751f\u5316\u3002\u56fe\u7247\u6765\u6e90\uff1aNat. 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