{"id":7364,"date":"2015-11-12T13:06:18","date_gmt":"2015-11-12T05:06:18","guid":{"rendered":"\/\/www.gsbet888.com\/?p=7364"},"modified":"2020-10-17T18:30:54","modified_gmt":"2020-10-17T10:30:54","slug":"post-7364","status":"publish","type":"post","link":"\/\/www.gsbet888.com\/chemists\/2015\/11\/post-7364.html","title":{"rendered":"\u8a79\u59ae\u5f17\u00b7\u675c\u5fb7\u7eb3 Jennifer Doudna"},"content":{"rendered":"<p>\u8a79\u59ae\u5f17\u00b7\u675c\u5fb7\u7eb3\uff08Jennifer Anne Doudna, 196x\u5e74xx\u6708xx\u65e5-\uff09\u662f\u7f8e\u56fd\u751f\u7269\u5316\u5b66\u5bb6\u3002\u52a0\u5dde\u5927\u5b66\u4f2f\u514b\u5229\u5206\u6821\u6559\u6388(<a href=\"http:\/\/rna.berkeley.edu\">\u7814\u7a76\u5ba4\u4e3b\u9875<\/a>\uff09\u3002<\/p>\n<h4>\u7ecf\u5386<\/h4>\n<p>1985 \u6ce2\u83ab\u7eb3\u5b66\u9662(Pomona College) \u6bd5\u4e1a<\/p>\n<p>1989 \u54c8\u4f5b\u5927\u5b66\u535a\u58eb\u5b66\u4f4d\uff08Jack W. Szostak\u6559\u6388\uff09<\/p>\n<p>1989 \u9ebb\u7701\u603b\u533b\u9662\u30fb\u54c8\u4f5b\u533b\u5b66\u9662\u535a\u58eb\u7814\u7a76\u5458(<a href=\"https:\/\/ja.wikipedia.org\/wiki\/%E3%82%B8%E3%83%A3%E3%83%83%E3%82%AF%E3%83%BBW%E3%83%BB%E3%82%B7%E3%83%A7%E3%82%B9%E3%82%BF%E3%82%AF\">Jack W. Szostak<\/a>\u6559\u6388\uff09<\/p>\n<p>1991 \u79d1\u7f57\u62c9\u591a\u5927\u5b66\u535a\u58eb\u7814\u7a76\u5458\uff08Lucille P. Markey Scholar\uff09(<a href=\"https:\/\/ja.wikipedia.org\/wiki\/%E3%83%88%E3%83%BC%E3%83%9E%E3%82%B9%E3%83%BB%E3%83%81%E3%82%A7%E3%83%83%E3%82%AF\">Thomas R. Cech<\/a>\u6559\u6388)<\/p>\n<p>1994 \u8036\u9c81\u5927\u5b66\u6559\u6388\uff08Henry Ford II Professor\uff09<\/p>\n<p>1997 \u970d\u534e\u5fb7\u00b7\u4f11\u65af\u533b\u5b66\u7814\u7a76\u6240\uff08HHMI\uff09\u7814\u7a76\u5458<\/p>\n<p>2003 \u52b3\u4f26\u65af\u4f2f\u514b\u5229\u56fd\u5bb6\u5b9e\u9a8c\u5ba4\u7814\u7a76\u5458<\/p>\n<p>2003 \u52a0\u5dde\u5927\u5b66\u4f2f\u514b\u5229\u5206\u6821\u6559\u6388<\/p>\n<p>&nbsp;<\/p>\n<h4>\u83b7\u5956\u7ecf\u5386<\/h4>\n<p>1996 A Searle Scholar 1996 Beckman Young Investigators Award 1999 NAS Award for Initiatives in Research 2000 Alan T. Waterman Award 2002 Elected to the National Academy of Sciences 2010 Elected to the Institute of Medicine 2014 Lurie Prize in Biomedical Sciences from the Foundation for the National Institutes of Health 2014 Dr. Paul Janssen Award for Biomedical Research and Breakthrough Prize in Life Sciences, (\u4e0eEmmanuelle Charpentier\u5171\u540c\u83b7\u5956\uff09 2015 Named one of Time Magazine\u2019s 100 most influential people in the world\uff08\u4e0eEmmanuelle Charpentier\u5171\u540c\u83b7\u5956\uff09 2015 Princess of Asturias Awards\uff08\u4e0eEmmanuelle Charpentier\u5171\u540c\u83b7\u5956\uff09 2015 Gruber Prize in Genetics\uff08\u4e0eEmmanuelle Charpentier\u5171\u540c\u83b7\u5956\uff09<\/p>\n<h4>\u7814\u7a76\u4e1a\u7ee9<\/h4>\n<p><strong>\u57fa\u56e0\u7ec4\u7f16\u8f91\u5de5\u5177CRISPR\/Cas9\u7684\u53d1\u73b0<\/strong> 2012\u5e74\u00a0Doudna\u4e0eCharpentier\u5171\u540c\u7814\u7a76\u5f00\u53d1\u4e86\u57fa\u56e0\u7ec4\u7f16\u8f91\u6280\u672fCISPR\/Cas9[1]\u3002\u662f\u7ee7ZFN\uff08Zinc Finger Nuclease\u3001\u3001TALEN\u3000(Transcription Activator-Like Effector Nuclease)\u540e\u7684\u7b2c\u4e09\u4ee3\u57fa\u56e0\u7ec4\u4fe1\u606f\u7684\u91cd\u5199\u6280\u672f\uff08\u53ef\u4efb\u610f\u6539\u53d8DNA\u78b1\u57fa\u5e8f\u5217\uff09\u8fd9\u4e00\u6280\u672f\u5907\u53d7\u77a9\u76ee\uff0c\u975e\u5e38\u53d7\u6b22\u8fce\u3002 <strong>CRISPR<\/strong>\u662fClustered Regularly Interspaced Short Palindromic Repeat\u7684\u7b80\u79f0\u30011987\u5e74\u5728\u5927\u962a\u5927\u5b66\u7684\u4e2d\u7530\u7be4\u7537\u540d\u8a89\u6559\u6388\u3001\u77f3\u91ce\u826f\u7d14\u6559\u6388\uff08\u73b0\u4e5d\u5dde\u5927\u5b66\u6559\u6388\uff09\u5728\u7814\u7a76\u5927\u80a0\u6746\u83cc\u7684DNA\u65f6\uff0c\u53d1\u73b0\u4e86\u4e00\u6bb5\u91cd\u590d\u6838\u82f7\u9178\u5e8f\u5217[2]\u3002\u5f53\u65f6\u5f97\u77e5\u8fd9\u4e2a\u5e8f\u5217\u662f\u5728\u539f\u6838\u751f\u7269\u4e2d\u4e00\u79cd\u5177\u6709\u514d\u75ab\u9632\u5fa1\u673a\u5236\u7684\u57fa\u56e0\u7ec4\uff0c\u6709\u5173\u5176\u8be6\u7ec6\u7684\u529f\u80fd\u5e76\u4e0d\u660e\u786e\u3002 Doudna\u4e0eCharpentier\u7684\u7814\u7a76\u7ec4\u6ce8\u610f\u5230\u4e86<strong>Cas9 (CRISPR associated protein 9)<\/strong>\u8fd9\u4e00\u79cd\u86cb\u767d\u8d28\u3002\u53d1\u73b0\u4e86\u5916\u6e90DNA\u901a\u8fc7Cas\u7ed3\u5408\u5728CRISPER\u3001\u4e0e\u5bfc\u5411RNA\u5f62\u6210\u7edc\u5408\u7269\uff0c\u5e76\u4e14\u5bfc\u5411\u6027RNA\u80fd\u591f\u5207\u65ad\u76f8\u5e94\u7684DNA\u53cc\u94fe\u8fd9\u4e00\u673a\u5236\u3002<\/p>\n<ol>\n<li>\u5bfc\u5411RNA\u4e0e\u76f8\u5e94\u7684DNA\u5f62\u6210\u4e8c\u91cd\u87ba\u65cb\uff0c\u539f\u6709\u7684DNA\u53cc\u94fe\u88ab\u89e3\u5f00\u3002<\/li>\n<li>Cas9\u5c06\u88ab\u89e3\u5f00\u7684\u4e24\u6761DNA\u94fe\u5207\u65ad<\/li>\n<li>\u57fa\u56e0\u7ec4DNA\u4e2d\u7684\u5207\u65ad\u90e8\u4f4d\u88ab\u4fee\u590d\u7684\u8fc7\u7a0b\u4e2d\u4f7f\u5f97DNA\u78b1\u57fa\u5e8f\u5217\u53d1\u751f\u53d8\u5316\u4ee5\u53ca\u57fa\u56e0\u7ec4\u4fe1\u606f\u91cd\u5199<\/li>\n<\/ol>\n<p>\u5c31\u662f\u8fd9\u6837\u7684\u4e00\u7cfb\u5217\u673a\u5236\uff0c\u53ef\u4ee5\u4f7f\u7528\u4e0d\u540c\u5bfc\u5411RNA\u3001\u5c06DNA\u7684\u4efb\u610f\u90e8\u4f4d\u5207\u65ad\u3002\u8fd8\u6709\u5c06\u8fd9\u4e2a\u65b9\u6cd5\u6539\u826f\u7684\u7814\u7a76\u4ee5\u53ca\u5229\u7528\u8fd9\u4e00\u673a\u5236\u7684\u7814\u7a76\u8fd1\u5e74\u6765\u975e\u5e38\u70ed\u95e8\u3001\u4e1a\u754c\u4eba\u58eb\u770b\u597d\u5b83\u6709\u53ef\u80fd\u4e00\u4e3e\u8363\u83b7\u8bfa\u8d1d\u5c14\u5956[3]\u3002 <a href=\"https:\/\/assets.www.gsbet888.com\/uploads\/2015\/11\/12.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-large wp-image-7420\" src=\"https:\/\/assets.www.gsbet888.com\/uploads\/2015\/11\/12-680x548.jpg\" alt=\"12\" width=\"680\" height=\"548\" srcset=\"https:\/\/assets.www.gsbet888.com\/uploads\/2015\/11\/12-680x548.jpg 680w, https:\/\/assets.www.gsbet888.com\/uploads\/2015\/11\/12-300x242.jpg 300w, https:\/\/assets.www.gsbet888.com\/uploads\/2015\/11\/12-150x120.jpg 150w, https:\/\/assets.www.gsbet888.com\/uploads\/2015\/11\/12.jpg 786w\" sizes=\"(max-width: 680px) 100vw, 680px\" \/><\/a><\/p>\n<h4>\u540d\u8a00\u96c6<\/h4>\n<p>&nbsp;<\/p>\n<h4>\u8bc4\u8bba\uff06\u5176\u4ed6<\/h4>\n<p>\u4eca\u5e74\u56e0\u4e3a\u5316\u5b66\u8bfa\u8d1d\u5c14\u5956\u9881\u7ed9DNA\u4fee\u590d\uff0c\u5316\u5b66\u5de5\u4f5c\u8005\u5e94\u8be5\u66f4\u4e86\u89e3\u751f\u547d\u5206\u5b50DNA\u7684\u57fa\u7840\u77e5\u8bc6\u548c\u524d\u6cbf\uff0c <a href=\"http:\/\/www.technologyreview.com\/featuredstory\/532796\/who-owns-the-biggest-biotech-discovery-of-the-century\/\">Who Owns the Biggest Biotech Discovery of the Century?<\/a> <a href=\"http:\/\/blog.sciencenet.cn\/blog-472747-723581.html\">CRISPR-Cas9\u57fa\u56e0\u6572\u9664\u6280\u672f\u4e86\u89e3\u5b66\u4e60<\/a> \u534e\u4eba\u9752\u5e74\u79d1\u5b66\u5bb6<a href=\"http:\/\/zlab.mit.edu\/index.html\">\u5f20\u950b<\/a>\u4e0eJennifer Doudna\u7b49\u4eba\u5728\u8be5\u9886\u57df\u7684\u53d1\u73b0\u6743\u4e89\u8bba<a href=\"http:\/\/www.zhihu.com\/question\/29956626\">\uff08\u77e5\u4e4e\uff09<\/a>\u00a0<a href=\"http:\/\/www.baike.com\/wiki\/\u5f20\u950b\">\u4e92\u52a8\u767e\u79d1<\/a><\/p>\n<p>&nbsp;<\/p>\n<h4>\u76f8\u5173\u52a8\u753b<\/h4>\n<p>Jennifer Doudna (UC Berkeley \/ HHMI): Genome Engineering with CRISPR-Cas9 <span class=\"embed-youtube\" style=\"text-align:center; display: block;\"><iframe loading=\"lazy\" class=\"youtube-player\" width=\"640\" height=\"360\" src=\"https:\/\/www.youtube.com\/embed\/SuAxDVBt7kQ?version=3&#038;rel=1&#038;showsearch=0&#038;showinfo=1&#038;iv_load_policy=1&#038;fs=1&#038;hl=en-US&#038;autohide=2&#038;wmode=transparent\" allowfullscreen=\"true\" style=\"border:0;\" sandbox=\"allow-scripts allow-same-origin allow-popups allow-presentation allow-popups-to-escape-sandbox\"><\/iframe><\/span> <span class=\"embed-youtube\" style=\"text-align:center; display: block;\"><iframe loading=\"lazy\" class=\"youtube-player\" width=\"640\" height=\"360\" src=\"https:\/\/www.youtube.com\/embed\/2pp17E4E-O8?version=3&#038;rel=1&#038;showsearch=0&#038;showinfo=1&#038;iv_load_policy=1&#038;fs=1&#038;hl=en-US&#038;autohide=2&#038;wmode=transparent\" allowfullscreen=\"true\" style=\"border:0;\" sandbox=\"allow-scripts allow-same-origin allow-popups allow-presentation allow-popups-to-escape-sandbox\"><\/iframe><\/span>\n<h4>\u76f8\u5173\u6587\u732e<\/h4>\n<ol>\n<li>Jinek, M.; Chylinski, K.; Fonfara, I.; Hauer, M.; Doudna, J. A.; Charpentier, E.;\u00a0<i>Science<\/i>\u00a0<b>2012<\/b>,\u00a0<i>337<\/i>, 816. DOI:\u00a0<a href=\"http:\/\/dx.doi.org\/10.1126\/science.1225829\">10.1126\/science.1225829<\/a><\/li>\n<li>Ishino, Y.; Shinagawa, H.; Makino, K.; Amemura, M.; Nakata, A.;<i>J. Bacteriol.<\/i>\u00a0<b>1987<\/b>,\u00a0<i>169<\/i>, 5429.<\/li>\n<li>Review: Doudna, J. A.; Charpentier, E.;<i>Science<\/i>\u00a0<b>2014<\/b>,\u00a0<i>346<\/i>, 6213. DOI:\u00a0<a href=\"http:\/\/dx.doi.org\/10.1126\/science.1258096\">10.1126\/science.1258096<\/a><\/li>\n<\/ol>\n<p><strong>\u672c\u6587\u7248\u6743\u5c5e\u4e8e\u00a0<\/strong><a href=\"\/\/www.gsbet888.com\/\"><strong>Chem-Station\u5316\u5b66\u7a7a\u95f4<\/strong><\/a><strong>\uff0c \u6b22\u8fce\u70b9\u51fb\u6309\u94ae\u5206\u4eab\uff0c\u672a\u7ecf\u8bb8\u53ef\uff0c\u8c22\u7edd\u8f6c\u8f7d<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"\u8a79\u59ae\u5f17\u00b7\u675c\u5fb7\u7eb3\uff08Jennifer Anne Doudna, 196x\u5e74xx\u6708xx\u65e5-\uff09\u662f\u7f8e\u56fd\u751f\u7269\u5316\u5b66\u5bb6\u3002\u52a0\u5dde\u5927\u5b66\u4f2f\u514b\u5229\u5206\u6821\u6559\u6388(\u7814\u7a76\u5ba4\u4e3b\u9875\uff09\u3002 \u7ecf\u5386 1985 \u6ce2\u83ab\u7eb3\u5b66\u9662(Pomona College) \u6bd5\u4e1a 1989 \u54c8\u4f5b\u5927\u5b66\u535a\u58eb\u5b66\u4f4d\uff08Jack W. Szostak\u6559\u6388\uff09 1989 \u9ebb\u7701\u603b\u533b\u9662\u30fb\u54c8\u4f5b\u533b\u5b66\u9662\u535a\u58eb\u7814\u7a76\u5458(Jack W. Szostak\u6559\u6388\uff09 1991 \u79d1\u7f57\u62c9\u591a\u5927\u5b66\u535a\u58eb\u7814\u7a76\u5458\uff08Lucille P. Markey Scholar\uff09(Thomas R. Cech\u6559\u6388) 1994 \u8036\u9c81\u5927\u5b66\u6559\u6388\uff08Henry Ford II Professor\uff09 1997 \u970d\u534e\u5fb7\u00b7\u4f11\u65af\u533b\u5b66\u7814\u7a76\u6240\uff08HHMI\uff09\u7814\u7a76\u5458 2003 \u52b3\u4f26\u65af\u4f2f\u514b\u5229\u56fd\u5bb6\u5b9e\u9a8c\u5ba4\u7814\u7a76\u5458 2003 \u52a0\u5dde\u5927\u5b66\u4f2f\u514b\u5229\u5206\u6821\u6559\u6388 &nbsp; \u83b7\u5956\u7ecf\u5386 1996 A Searle Scholar 1996 Beckman Young Investigators Award 1999 NAS Award for Initiatives in Research 2000 Alan T. Waterman Award 2002 Elected to the National Academy of Sciences 2010 Elected to the Institute of Medicine 2014 Lurie Prize in Biomedical Sciences from the Foundation for the National Institutes of Health 2014 Dr. Paul Janssen Award for Biomedical Research and Breakthrough Prize in Life Sciences, (\u4e0eEmmanuelle Charpentier\u5171\u540c\u83b7\u5956\uff09 2015 Named one of Time Magazine\u2019s [&hellip;]","protected":false},"author":3,"featured_media":7418,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","enabled":false},"version":2}},"categories":[212],"tags":[1116,1114,1115],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"https:\/\/assets.www.gsbet888.com\/uploads\/2015\/11\/2015-09-25_15-51-41.png","jetpack_shortlink":"https:\/\/wp.me\/p96op1-1UM","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/posts\/7364"}],"collection":[{"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/comments?post=7364"}],"version-history":[{"count":3,"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/posts\/7364\/revisions"}],"predecessor-version":[{"id":35448,"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/posts\/7364\/revisions\/35448"}],"wp:featuredmedia":[{"embeddable":true,"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/media\/7418"}],"wp:attachment":[{"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/media?parent=7364"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/categories?post=7364"},{"taxonomy":"post_tag","embeddable":true,"href":"\/\/www.gsbet888.com\/wp-json\/wp\/v2\/tags?post=7364"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}